Label-free electrical detection of DNA by means of field-effect nanoplate capacitors experiments and modeling
- Label-free electrical detection of consecutive deoxyribonucleic acid (DNA) hybridization/denaturation by means of an array of individually addressable field-effect-based nanoplate silicon-on-insulator (SOI) capacitors modified with gold nanoparticles (Au-NP) is investigated. The proposed device detects charge changes on Au-NP/DNA hybrids induced by the hybridization or denaturation event. DNA hybridization was performed in a high ionic-strength solution to provide a high hybridization efficiency. On the other hand, to reduce the screening of the DNA charge by counter ions and to achieve a high sensitivity, the sensor signal induced by the hybridization and denaturation events was measured in a low ionic-strength solution. High sensor signals of about 120, 90, and 80 mV were registered after the DNA hybridization, denaturation, and re-hybridization events, respectively. Fluorescence microscopy has been applied as reference method to verify the DNA immobilization, hybridization, and denaturation processes. An electrostatic charge-planeLabel-free electrical detection of consecutive deoxyribonucleic acid (DNA) hybridization/denaturation by means of an array of individually addressable field-effect-based nanoplate silicon-on-insulator (SOI) capacitors modified with gold nanoparticles (Au-NP) is investigated. The proposed device detects charge changes on Au-NP/DNA hybrids induced by the hybridization or denaturation event. DNA hybridization was performed in a high ionic-strength solution to provide a high hybridization efficiency. On the other hand, to reduce the screening of the DNA charge by counter ions and to achieve a high sensitivity, the sensor signal induced by the hybridization and denaturation events was measured in a low ionic-strength solution. High sensor signals of about 120, 90, and 80 mV were registered after the DNA hybridization, denaturation, and re-hybridization events, respectively. Fluorescence microscopy has been applied as reference method to verify the DNA immobilization, hybridization, and denaturation processes. An electrostatic charge-plane model for potential changes at the gate surface of a nanoplate field-effect sensor induced by the DNA hybridization has been developed taking into account both the Debye length and the distance of the DNA charge from the gate surface.…
Verfasserangaben: | Maryam H. Abouzar, Arshak Poghossian, Andrey G. CherstvyORCiD, Angela M. Pedraza, Sven Ingebrandt, Michael J. Schöning |
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DOI: | https://doi.org/10.1002/pssa.201100710 |
ISSN: | 1862-6300 |
Titel des übergeordneten Werks (Englisch): | Physica status solidi : A, Applications and materials science |
Verlag: | Wiley-VCH |
Verlagsort: | Weinheim |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2012 |
Erscheinungsjahr: | 2012 |
Datum der Freischaltung: | 26.03.2017 |
Freies Schlagwort / Tag: | DNA; field-effect; gold nanoparticle; label-free detection; nanoplate capacitor |
Band: | 209 |
Ausgabe: | 5 |
Seitenanzahl: | 10 |
Erste Seite: | 925 |
Letzte Seite: | 934 |
Fördernde Institution: | Deutsche Forschungsgemeinschaft (DFG) [CH 707/5-1] |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Physik und Astronomie |
Peer Review: | Referiert |