David J. Poxson, Michal Karady, Roger Gabrielsson, Aziz Y. Alkattan, Anna Gustavsson, Siamsa M. Doyle, Stephanie Robert, Karin Ljung, Markus Grebe, Daniel T. Simon, Magnus Berggren
- The organic electronic ion pump (OEIP) provides flow-free and accurate delivery of small signaling compounds at high spatio-temporal resolution. To date, the application of OEIPs has been limited to delivery of nonaromatic molecules to mammalian systems, particularly for neuroscience applications. However, many long-standing questions in plant biology remain unanswered due to a lack of technology that precisely delivers plant hormones, based on cyclic alkanes or aromatic structures, to regulate plant physiology. Here, we report the employment of OEIPs for the delivery of the plant hormone auxin to induce differential concentration gradients and modulate plant physiology. We fabricated OEIP devices based on a synthesized dendritic polyelectrolyte that enables electrophoretic transport of aromatic substances. Delivery of auxin to transgenic Arabidopsis thaliana seedlings in vivo was monitored in real time via dynamic fluorescent auxin-response reporters and induced physiological responses in roots. Our results provide a starting pointThe organic electronic ion pump (OEIP) provides flow-free and accurate delivery of small signaling compounds at high spatio-temporal resolution. To date, the application of OEIPs has been limited to delivery of nonaromatic molecules to mammalian systems, particularly for neuroscience applications. However, many long-standing questions in plant biology remain unanswered due to a lack of technology that precisely delivers plant hormones, based on cyclic alkanes or aromatic structures, to regulate plant physiology. Here, we report the employment of OEIPs for the delivery of the plant hormone auxin to induce differential concentration gradients and modulate plant physiology. We fabricated OEIP devices based on a synthesized dendritic polyelectrolyte that enables electrophoretic transport of aromatic substances. Delivery of auxin to transgenic Arabidopsis thaliana seedlings in vivo was monitored in real time via dynamic fluorescent auxin-response reporters and induced physiological responses in roots. Our results provide a starting point for technologies enabling direct, rapid, and dynamic electronic interaction with the biochemical regulation systems of plants.…
MetadatenAuthor details: | David J. Poxson, Michal Karady, Roger GabrielssonORCiD, Aziz Y. Alkattan, Anna Gustavsson, Siamsa M. Doyle, Stephanie Robert, Karin LjungORCiD, Markus GrebeORCiDGND, Daniel T. Simon, Magnus BerggrenORCiD |
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DOI: | https://doi.org/10.1073/pnas.1617758114 |
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ISSN: | 0027-8424 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/28420793 |
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Title of parent work (English): | Proceedings of the National Academy of Sciences of the United States of America |
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Publisher: | National Acad. of Sciences |
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Place of publishing: | Washington |
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Publication type: | Article |
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Language: | English |
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Year of first publication: | 2017 |
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Publication year: | 2017 |
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Release date: | 2020/04/20 |
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Tag: | Arabidopsis thaliana; auxin; bioelectronics; dendritic polymer; polyelectrolyte |
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Volume: | 114 |
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Number of pages: | 6 |
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First page: | 4597 |
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Last Page: | 4602 |
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Funding institution: | Knut and Alice Wallenberg Foundation ShapeSystems project [KAW 2012.0050]; Swedish Foundation for Strategic Research [RMA-11:0104] |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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Peer review: | Referiert |
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