Side-directed transfer and presystemic metabolism of selenoneine in a human intestinal barrier model
- Scope: Selenoneine, a recently discovered selenium (Se) species mainly present in marine fish, is the Se analogue of ergothioneine, a sulfur-containing purported antioxidant. Although similar properties have been proposed for selenoneine, data on its relevance to human health are yet scarce. Here, the transfer and presystemic metabolism of selenoneine in an in vitro model of the human intestinal barrier are investigated. Methods and results: Selenoneine and the reference species Se-methylselenocysteine (MeSeCys) and selenite are applied to the Caco-2 intestinal barrier model. Selenoneine is transferred in higher amounts, but with similar kinetics as selenite, while MeSeCys shows the highest permeability. In contrast to the reference species, transfer of selenoneine is directed toward the blood side. Cellular Se contents demonstrate that selenoneine is efficiently taken up by Caco-2 cells. Moreover, HPLC/MS-based Se speciation studies reveal a partial metabolism to Se-methylselenoneine, a metabolite previously detected in human bloodScope: Selenoneine, a recently discovered selenium (Se) species mainly present in marine fish, is the Se analogue of ergothioneine, a sulfur-containing purported antioxidant. Although similar properties have been proposed for selenoneine, data on its relevance to human health are yet scarce. Here, the transfer and presystemic metabolism of selenoneine in an in vitro model of the human intestinal barrier are investigated. Methods and results: Selenoneine and the reference species Se-methylselenocysteine (MeSeCys) and selenite are applied to the Caco-2 intestinal barrier model. Selenoneine is transferred in higher amounts, but with similar kinetics as selenite, while MeSeCys shows the highest permeability. In contrast to the reference species, transfer of selenoneine is directed toward the blood side. Cellular Se contents demonstrate that selenoneine is efficiently taken up by Caco-2 cells. Moreover, HPLC/MS-based Se speciation studies reveal a partial metabolism to Se-methylselenoneine, a metabolite previously detected in human blood and urine. Conclusions: Selenoneine is likely to pass the intestinal barrier via transcellular, carrier-mediated transport, is highly bioavailable to Caco-2 cells and undergoes metabolic transformations. Therefore, further studies are needed to elucidate its possible health effects and to characterize the metabolism of selenoneine in humans.…
Author details: | Isabelle RohnGND, Nina KroepflORCiD, Julia BornhorstORCiDGND, Doris KühneltORCiD, Tanja SchwerdtleORCiDGND |
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DOI: | https://doi.org/10.1002/mnfr.201900080 |
ISSN: | 1613-4125 |
ISSN: | 1613-4133 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/30939220 |
Title of parent work (English): | Molecular nutrition & food research : bioactivity, chemistry, immunology, microbiology, safety, technology |
Publisher: | Wiley |
Place of publishing: | Hoboken |
Publication type: | Article |
Language: | English |
Date of first publication: | 2019/04/02 |
Publication year: | 2019 |
Release date: | 2021/01/28 |
Tag: | Caco-2 intestinal barrier model; Se-methylselenoneine; bioavailability; presystemic metabolism; selenoneine |
Volume: | 63 |
Issue: | 12 |
Number of pages: | 11 |
Funding institution: | German Research Foundation (DFG)German Research Foundation (DFG) [SCHW 903/9-1]; Austrian Science Fund (FWF)Austrian Science Fund (FWF) [I 2262-N28]; DFGGerman Research Foundation (DFG) [FOR 2558] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft |
DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer review: | Referiert |