Wiring of the aldehyde oxidoreductase PaoABC to electrode surfaces via entrapment in low potential phenothiazine-modified redox polymers
- Phenothiazine-modified redox hydrogels were synthesized and used for the wiring of the aldehyde oxidoreductase PaoABC to electrode surfaces. The effects of the pH value and electrode surface modification on the biocatalytic activity of the layers were studied in the presence of vanillin as the substrate. The enzyme electrodes were successfully employed as bioanodes in vanillin/O-2 biofuel cells in combination with a high potential bilirubin oxidase biocathode. Open circuit voltages of around 700 mV could be obtained in a two compartment biofuel cell setup. Moreover, the use of a rather hydrophobic polymer with a high degree of crosslinking sites ensures the formation of stable polymer/enzyme films which were successfully used as bioanode in membrane-less biofuel cells. (C) 2015 Elsevier B.V. All rights reserved.
Author details: | Piyanut Pinyou, Adrian Ruff, Sascha Poeller, Sabine Alsaoub, Silke LeimkühlerORCiDGND, Ursula WollenbergerORCiDGND, Wolfgang Schuhmann |
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DOI: | https://doi.org/10.1016/j.bioelechem.2015.12.005 |
ISSN: | 1567-5394 |
ISSN: | 1878-562X |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/26775204 |
Title of parent work (English): | Bioelectrochemistry : an international journal devoted to electrochemical aspects of biology and biological aspects of electrochemistry ; official journal of the Bioelectrochemical Society |
Publisher: | Elsevier |
Place of publishing: | Lausanne |
Publication type: | Article |
Language: | English |
Year of first publication: | 2016 |
Publication year: | 2016 |
Release date: | 2020/03/22 |
Tag: | Aldehyde oxidoreductase; Biofuel cell; Biosensor; Enzyme electrode; Phenothiazine; Redox polymer |
Volume: | 109 |
Number of pages: | 7 |
First page: | 24 |
Last Page: | 30 |
Funding institution: | Deutsche Forschungsgemeinschaft [EXC 1069, EXC 314]; BMBF [03IS2201F]; Deutscher Akademischer Austauschdienst (DAAD) |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
Peer review: | Referiert |