Co-delivery of genes can be confounded by bicistronic vector design
- Maximizing the efficiency of nanocarrier-mediated co-delivery of genes for co-expression in the same cell is critical for many applications. Strategies to maximize co-delivery of nucleic acids (NA) focused largely on carrier systems, with little attention towards payload composition itself. Here, we investigated the effects of different payload designs: co-delivery of two individual "monocistronic" NAs versus a single bicistronic NA comprising two genes separated by a 2A self-cleavage site. Unexpectedly, co-delivery via the monocistronic design resulted in a higher percentage of co-expressing cells, while predictive co-expression via the bicistronic design remained elusive. Our results will aid the application-dependent selection of the optimal methodology for co-delivery of genes.
Verfasserangaben: | Hanieh MoradianORCiDGND, Manfred GossenORCiDGND, Andreas LendleinORCiDGND |
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DOI: | https://doi.org/10.1557/s43579-021-00128-7 |
ISSN: | 2159-6859 |
ISSN: | 2159-6867 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/35223145 |
Titel des übergeordneten Werks (Englisch): | MRS Communications |
Verlag: | Springer |
Verlagsort: | Heidelberg |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Erstveröffentlichung: | 18.02.2022 |
Erscheinungsjahr: | 2022 |
Datum der Freischaltung: | 08.06.2023 |
Freies Schlagwort / Tag: | Molecular; Packaging; Protein |
Band: | 12 |
Ausgabe: | 2 |
Seitenanzahl: | 9 |
Erste Seite: | 145 |
Letzte Seite: | 153 |
Fördernde Institution: | Helmholtz Association; Helmholtz Graduate School of Macromolecular; Bioscience (MacroBio) [VH-GS-503]; German Federal Ministry of Education; and Research through the Program Health Research [13GW0098] |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Peer Review: | Referiert |
Publikationsweg: | Open Access / Hybrid Open-Access |
Lizenz (Deutsch): | CC-BY - Namensnennung 4.0 International |