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Saccharomyces cerevisiae possesses two glycogenin isoforms (designated as Glg1p and Glg2p) that both contain a conserved tyrosine residue, Tyr232. However, Glg2p possesses an additional tyrosine residue, Tyr230 and therefore two potential autoglucosylation sites. Glucosylation of Glg2p was studied using both matrix-assisted laser desorption ionization and electrospray quadrupole time of flight mass spectrometry. Glg2p, carrying a C-terminal (His(6)) tag, was produced in Escherichia coli and purified. By tryptic digestion and reversed phase chromatography a peptide (residues 219-246 of the complete Glg2p sequence) was isolated that contained 4-25 glucosyl residues. Following incubation of Glg2p with UDPglucose, more than 36 glucosyl residues were covalently bound to this peptide. Using a combination of cyanogen bromide cleavage of the protein backbone, enzymatic hydrolysis of glycosidic bonds and reversed phase chromatography, mono- and diglucosylated peptides having the sequence PNYGYQSSPAM were generated. MS/MS spectra revealed that glucosyl residues were attached to both Tyr232 and Tyr230 within the same peptide. The formation of the highly glucosylated eukaryotic Glg2p did not favour the bacterial glycogen accumulation. Under various experimental conditions Glg2p-producing cells accumulated approximately 30% less glycogen than a control transformed with a Glg2p lacking plasmid. The size distribution of the glycogen and extractable activities of several glycogen-related enzymes were essentially unchanged. As revealed by high performance anion exchange chromatography, the intracellular maltooligosaccharide pattern of the bacterial cells expressing the functional eukaryotic transgene was significantly altered. Thus, the eukaryotic glycogenin appears to be incompatible with the bacterial initiation of glycogen biosynthesis
Writing travel, writing life
(2022)
The book compares the texts of three Swiss authors: Ella Maillart, Annemarie Schwarzenbach and Nicolas Bouvier. The focus is on their trip from Genève to Kabul that Ella Maillart and Annemarie Schwarzenbach made together in 1939/1940 and Nicolas Bouvier 1953/1954 with the artist Thierry Vernet. The comparison shows the strong connection between the journey and life and between ars vivendi and travel literature.
This book also gives an overview of and organises the numerous terms, genres, and categories that already exist to describe various travel texts and proposes the new term travelling narration. The travelling narration looks at the text from a narratological perspective that distinguishes the author, narrator, and protagonist within the narration.
In the examination, ten motifs could be found to characterise the travelling narration: Culture, Crossing Borders, Freedom, Time and Space, the Aesthetics of Landscapes, Writing and Reading, the Self and/as the Other, Home, Religion and Spirituality as well as the Journey. The importance of each individual motif does not only apply in the 1930s or 1950s but also transmits important findings for living together today and in the future.
Would the world be a better place if one were to adopt a European approach to state immunity?
(2021)
This chapter argues not only that there is no European Sonderweg (or ‘special way’) when it comes to the law of state immunity but that there ought not to be one. Debates within The Hague Conference on Private International Law in the late 1990s and those leading to the adoption of the 2002 UN Convention on Jurisdictional Immunities of States, as well as the development of the EU Brussels Regulation on Jurisdiction and Enforcement, as amended in 2015, all demonstrate that state immunity was not meant to be limited by such treaties but ‘safeguarded’. Likewise, there is no proof that regional European customary law limits state immunity when it comes to ius cogens violations, as Italy and (partly) Greece are the only European states denying state immunity in such cases while the European Court of Human Rights has, time and again, upheld a broad concept of state immunity. It therefore seems unlikely that in the foreseeable future a specific European customary law norm on state immunity will develop, especially given the lack of participation in such practice by those states most concerned by the matter, including Germany. This chapter considers the possible legal implications of the jurisprudence of the Italian Constitutional Court for European military operations (if such operations went beyond peacekeeping). These implications would mainly depend on the question of attribution: if one where to assume that acts undertaken within the framework of military operations led by the EU were to be, at least also, attributable to the troop-contributing member states, the respective troop-contributing state would be entitled to enjoy state immunity exactly to the same degree as in any kind of unilateral military operations. Additionally, some possible perspectives beyond Sentenza 238/2014 are examined, in particular concerning the redress awarded by domestic courts ‘as long as’ neither the German nor the international system grant equivalent protection to the victims of serious violations of international humanitarian law committed during World War II. In the author’s opinion, strengthening the jurisdiction of international courts and tribunals, bringing interstate cases for damages before the International Court of Justice, as well as providing for claims commissions where individual compensation might be sought for violations of international humanitarian law would be more useful and appropriate mechanisms than denying state immunity.
Workplace friendships
(2023)
Workplace friendships, i.e., when work colleagues are also friends, are a widespread phenomenon in organizations which has attracted increasing research interest in recent decades. Numerous studies have investigated consequences of workplace friendships and found positive outcomes, such as increased employee job satisfaction or organizational performance, as well as negative outcomes, such as decreased knowledge-sharing between different friendship cliques. Other studies have examined what shapes workplace friendships, focusing on determinants such as personality or the spatial composition of organizations. Finally, an increasing number of studies focus on multiplex workplace friendships, where employees who are friends are also linked by a specific work-focused relationship. In this chapter, we first take stock of the literature on workplace friendships by providing an overview of their antecedents and consequences at the individual, the group, and the organizational level, and review the smaller body of research on multiplex workplace friendships. Second, we critically discuss practical implications of workplace friendships, focusing on their relevance to three current challenges for employees and organizations: the increase in virtual work, social inequalities in organizations, and the increased overlap of professional and private life. Finally, we provide recommendations for organizations on how to address these challenges and effectively manage workplace friendships.
The great Old English epic 'Beowulf' has been dated to practically every century between the 6th and the 11th century, depending on the criteria of dating adopted and the approaches advocated by the respective scholars. As the text successfully avoids to provide definite cues or evidence for a definitive date, these scholarly attempts reveal more about the respective scholars' research interests than offering uncontroversial dates. The point of dating 'Beowulf' then seems to provide scholars with the opportunity to anchor their own personal understanding of the poem within the century of their own personal predilection.
Disinformation campaigns spread rapidly through social media and can cause serious harm, especially in crisis situations, ranging from confusion about how to act to a loss of trust in government institutions. Therefore, the prevention of digital disinformation campaigns represents an important research topic. However, previous research in the field of information systems focused on the technical possibilities to detect and combat disinformation, while ethical and legal perspectives have been neglected so far. In this article, we synthesize previous information systems literature on disinformation prevention measures and discuss these measures from an ethical and legal perspective. We conclude by proposing questions for future research on the prevention of disinformation campaigns from an IS, ethical, and legal perspective. In doing so, we contribute to a balanced discussion on the prevention of digital disinformation campaigns that equally considers technical, ethical, and legal issues, and encourage increased interdisciplinary collaboration in future research.
We and AI
(2021)
Using novel longitudinal data, this paper studies the short- and medium-term effects of Russia's invasion of Ukraine on February 24, 2022 on social trust of adolescents in Germany. Comparing adolescents who responded to our survey shortly before the start of the war with those who responded shortly after the conflict began and applying difference-in-differences (DiD) models over time, we find a significant decline in the outcome after the war started. These findings provide new evidence on how armed conflicts influence social trust and well-being among young people in a country not directly involved in the war.
Voided space-charge electrets : piezoelectric transducer materials for electro-acoustic applications
(2004)
Visual Social Networking Sites (SNSs) enable users to present themselves favorably to gain likes and the attention of others. Especially, Instagram is known for its focus on beauty, fitness, fashion, and dietary topics. Although a large body of research reports negative weight-related outcomes of SNS usage (e.g., body dissatisfaction, body image concerns), studies examining how SNS usage relates to these outcomes are scarce. Based on the visual normalization theory, we argue that SNS content facilitates normalization of so-called thin- and fit-ideals, thereby leading to biased perceptions of the average body weight in society. Therefore, this study tests whether Instagram use is associated with perceiving that the average person weighs less. Responses of 181 survey participants confirm that Instagram use is negatively related to average weight perception of both women and men. These findings contribute to the growing body of research on how SNS use relates to negative weight-related outcomes.
Visual Culture
(2004)
The management of knowledge in organizations considers both established long-term
processes and cooperation in agile project teams. Since knowledge can be both tacit and explicit, its transfer from the individual to the organizational knowledge base poses a challenge in organizations. This challenge increases when the fluctuation of knowledge carriers is exceptionally high. Especially in large projects in which external consultants are involved, there is a risk that critical, company-relevant knowledge generated in the project will leave the company with the external knowledge carrier and thus be lost. In this paper, we show the advantages of an early warning system for knowledge management to avoid this loss. In particular, the potential of visual analytics in the context of knowledge management systems is presented and discussed. We present a project for the development of a business-critical software system and discuss the first implementations and results.
In the last two centuries BC, with the Republic limping towards its end, the cultivated ruling elite began to lose its moral and political authority.1 Its members not only held themselves responsible for the so-called crisis of tradition, but at the same time also conveyed the impression of a loss of memory, as if all Romans were suffering from some kind of amnesia or identity crisis.2 In particular, institutional figures such as pontiffs and augurs, who had preserved Rome’s memory throughout its history, were accused of neglecting their duties and, by extension, of allowing ancient practices and values to slowly disappear.3 Accordingly, Cicero and Varro, both perfect representatives of this elite, employed recurrent terms such as neglect (neglegentia/neglegere), involuntary abandon (amittere), oblivion (oblivio), vanishing of institutions (evanescere), and ignorance (ignoratio/ignorare) to describe this critical loss of information; they depicted the citizenry of Rome (civitas) as disoriented and estranged, incapable of sharing any common knowledge or values.
The contribution explores how an understanding of neoliberal subjectification in socio-economic education can serve to counteract the trend marketisation of democracy. Drawing on Foucault’s lectures on biopolitics and Brown’s current analysis of neoliberalism, it lays out a sociological explanation that treats the idea of homo economicus as a structuring element of our society and outlines the threat this poses to the liberal democratic order. The second part of the contribution outlines – through immanent critique – an ideology-critical analytical competence that uses key problems to illuminate socially critical perspectives on social reality. The objective is to challenge some of the foundations of social order (Salomon, D. Kritische politische Bildung. Ein Versuch. In B. Widmaier & Overwien, B. (Hrsg.), Was heißt heute kritische politische Bildung? (S. 232–239). Wochenschau, 2013) in pursuit of the ultimate objective of an educated and assertive citizenry.
Like conventional software projects, projects in model-driven software engineering require adequate management of multiple versions of development artifacts, importantly allowing living with temporary inconsistencies. In the case of model-driven software engineering, employed versioning approaches also have to handle situations where different artifacts, that is, different models, are linked via automatic model transformations.
In this report, we propose a technique for jointly handling the transformation of multiple versions of a source model into corresponding versions of a target model, which enables the use of a more compact representation that may afford improved execution time of both the transformation and further analysis operations. Our approach is based on the well-known formalism of triple graph grammars and a previously introduced encoding of model version histories called multi-version models. In addition to showing the correctness of our approach with respect to the standard semantics of triple graph grammars, we conduct an empirical evaluation that demonstrates the potential benefit regarding execution time performance.
International law is constantly navigating the tension between preserving the status quo and adapting to new exigencies. But when and how do such adaptation processes give way to a more profound transformation, if not a crisis of international law? To address the question of how attacks on the international legal order are changing the value orientation of international law, this book brings together scholars of international law and international relations. By combining theoretical and methodological analyses with individual case studies, this book offers readers conceptualizations and tools to systematically examine value change and explore the drivers and mechanisms of these processes. These case studies scrutinize value change in the foundational norms of the post-1945 order and in norms representing the rise of the international legal order post-1990. They cover diverse issues: the prohibition of torture, the protection of women’s rights, the prohibition of the use of force, the non-proliferation of nuclear weapons, sustainability norms, and accountability for core international crimes. The challenges to each norm, the reactions by norm defenders, and the fate of each norm are also studied. Combined, the analyses show that while a few norms have remained surprisingly robust, several are changing, either in substance or in legal or social validity. The book concludes by integrating the conceptual and empirical insights from this interdisciplinary exchange to assess and explain the ambiguous nature of value change in international law beyond the extremes of mere progress or decline.
Artificial intelligence (AI)-based technologies can increasingly perform knowledge work tasks, such as medical diagnosis. Thereby, it is expected that humans will not be replaced by AI but work closely with AI-based technology (“augmentation”). Augmentation has ethical implications for humans (e.g., impact on autonomy, opportunities to flourish through work), thus, developers and managers of AI-based technology have a responsibility to anticipate and mitigate risks to human workers. However, doing so can be difficult as AI encompasses a wide range of technologies, some of which enable fundamentally new forms of interaction. In this research-in-progress paper, we propose the development of a taxonomy to categorize unique characteristics of AI-based technology that influence the interaction and have ethical implications for human workers. The completed taxonomy will support researchers in forming cumulative knowledge on the ethical implications of augmentation and assist practitioners in the ethical design and management of AI-based technology in knowledge work.
This study aims to bring together scattered research findings on user satisfaction with mobile government apps into a unified framework. The researchers analyzed 70 high-quality papers from leading journals and conferences and systematically integrated different frameworks and case studies to reflect the importance of the field over time while also highlighting methodological and geographical research gaps. The study achieved a significant methodological advance by developing codebooks for empirical analysis utilizing the App Store. This approach validated the framework’s dimensions on 8,524 reviews, demonstrating the framework’s applicability to platform-based apps and identifying critical areas for future research. Combining academic insights with practical findings, this research provides comprehensive guidance for developing and evaluating user-centered mobile government apps, facilitating improved service delivery and alignment with user expectations.
Scrollytellings are an innovative form of web content. Combining the benefits of books, images, movies, and video games, they are a tool to tell compelling stories and provide excellent learning opportunities. Due to their multi-modality, creating high-quality scrollytellings is not an easy task. Different professions, such as content designers, graphics designers, and developers, need to collaborate to get the best out of the possibilities the scrollytelling format provides. Collaboration unlocks great potential. However, content designers cannot create scrollytellings directly and always need to consult with developers to implement their vision. This can result in misunderstandings. Often, the resulting scrollytelling will not match the designer’s vision sufficiently, causing unnecessary iterations. Our project partner Typeshift specializes in the creation of individualized scrollytellings for their clients. Examined existing solutions for authoring interactive content are not optimally suited for creating highly customized scrollytellings while still being able to manipulate all their elements programmatically. Based on their experience and expertise, we developed an editor to author scrollytellings in the lively.next live-programming environment. In this environment, a graphical user interface for content design is combined with powerful possibilities for programming behavior with the morphic system. The editor allows content designers to take on large parts of the creation process of scrollytellings on their own, such as creating the visible elements, animating content, and fine-tuning the scrollytelling. Hence, developers can focus on interactive elements such as simulations and games. Together with Typeshift, we evaluated the tool by recreating an existing scrollytelling and identified possible future enhancements. Our editor streamlines the creation process of scrollytellings. Content designers and developers can now both work on the same scrollytelling. Due to the editor inside of the lively.next environment, they can both work with a set of tools familiar to them and their traits. Thus, we mitigate unnecessary iterations and misunderstandings by enabling content designers to realize large parts of their vision of a scrollytelling on their own. Developers can add advanced and individual behavior. Thus, developers and content designers benefit from a clearer distribution of tasks while keeping the benefits of collaboration.
Tieken-Boon van Ostade, I. (Hrsg.), Two Hundred Years of Lindley Murray; Münster, Nodus, 1996
(1998)
Agricultural production worldwide has been increasing in the last decades at a very fast pace and with it the waste generation. Livestock activities are one of the largest producers of residues in the agricultural sector and contribute greatly to climate change. The present chapter gives an introduction and an in-depth analysis of the waste management of livestock for the conversion in a circular agriculture and economy based on research and experience in the sector conducted in the last decades. The conversion of animal waste into energy generation is an opportunity for farmers to obtain additional economic benefits, while contributing to the environment by preventing the release of GHGs into the atmosphere. The use of animal waste for energy generation through anaerobic digestion is a progressive technique and is being widely accepted in Europe, where Germany is the leading country in the use of biogas plants for energy production among others in the European Union. Economically speaking, the livestock industry faces the challenge of converting its production into a clean and more profitable production. The goal of this chapter is to analyze the economic benefit as well as the environmental contribution and future challenges of the use of livestock waste in the biorefineries sector from different perspectives, based on an intensive literature review. This review is accompanied by a geospatial analysis component, mapping biogas reactor hotspots and clusters in Germany, by means of methods of spatial statistics as analysis methods as kernel density estimations (KDE) and K-means clustering, based on volunteer geographic data. The applied methods easily can be transferred to other regions and allow a quick macroscopic overview over existing biogas reactors; furthermore, an identification of cluster and hotspots with a high biogas potential, that in a subsequent step can be analyzed in depth in larger scales.
Although mothers and fathers in almost all rich democracies are entitled to some form of paid parenting leave, fathers in particular do not take all the leave available to them. As employers play an important role in the implementation of parenting leave policies, this chapter investigates which workplace characteristics influence mothers' and fathers' uptake of their statutory leave entitlements. In Part 1, we estimate the size of the gap between statutory leave entitlement and leave uptake across genders and countries by combining data from the OECD and the European Labor Force Survey. In Parts 2 and 3, we review the literature on structural, cultural, and normative explanations for the gap in parenting leave uptake. We conclude the chapter with suggestions for further research, including the need for reliable data on the size of the implementation gap and research on non-European countries.
The power of opposition
(2022)
Proposing a novel way to look at the consolidation of democratic regimes, this book presents important theoretical and empirical contributions to the study of democratic consolidation, legislative organization, and public opinion.
Theoretically, Simone Wegmann brings legislatures into focus as the main body representing both winners and losers of democratic elections. Empirically, Wegmann shows that the degree of policy-making power of opposition players varies considerably between countries. Using survey data from the CSES, the ESS, and the LAPOP and systematically analyzing more than 50 legislatures across the world and the specific rights they grant to opposition players during the policy-making process, Wegmann demonstrates that neglecting the curial role of the legislature in a democratic setting can only lead to an incomplete assessment of the importance of institutions for democratic consolidation.
The Power of Opposition will be of great interest to scholars of comparative politics, especially those working on questions related to legislative organization, democratic consolidation, and/or public opinion.
The politics of fear
(2022)
Administrative reforms refer to conscious decisions about institution building and institutional change that are taken at the end of political processes and can be conceived as the attempt by politico-administrative actors to change the institutional order (polity) within which they make and implement decisions. In this paper we proceed from the assumption that the role of politics, the constellation of political actors and arenas vary according to the scope and objectives of administrative reforms. Depending on whether they refer to changes between organizational units/levels/sectors ('external institutional policy') or to an internal reorganization ('internal institutional policy'), different actor strategies, patterns of conflict and power constellations can be expected. As external administrative reforms are aimed at changing functional and/or territorial jurisdictions and thus always involve external actors, larger resistance, heavier political conflicts and generally more politicization are likely to occur than in the case of internal administrative reforms. Yet, for internal reforms, too, actor coalitions which support or block institutional changes, promotors, leaders, and moderators have revealed to shape processes and outcomes. Against this background, this chapter examines the influence of politics on various types of administrative reforms making a distinction between external and internal institutional policies. We analyse the role of politico-administrative actors, their strategies and influence on the formulation, trajectories and outcomes of administrative reforms. Our major focus will be on reforms in the multi-level system on the one hand and on (Post-) NPM reforms on the other as two major international trends. Drawing on reform experiences in different European countries, the chapter will reveal to what extent actors' interests and influences have triggered and shaped administrative reforms and which difference these have made for the reform outcome.
The perceived risks and benefits of genetically modified food products : Experts versus consumers
(1999)
The increasing prevalence and ubiquity of digital technologies is changing the needs and expectations of patients towards healthcare services. As a result, a plethora of patient-centered services edges into the healthcare market. Since digital technologies bear the potential to surmount barriers in time and space, patients increasingly demand real-time or near-time healthcare services. Amongst a cloud of related concepts in the context of digital health, one term increasingly typifies this impulse: on-demand healthcare. While this term can be noticeably found in practice, there is hardly some theoretical foundation so far. Against this background, the aim of this paper is to address this research gap and to explore the phenomenon of on-demand healthcare. Based on a design-science approach including a literature review and analysis of in-depth interviews and empirical cases, the outcome of this paper is twofold: (1) a conceptual framework and (2) a proposal for a definition of on-demand healthcare.
The EU and its member countries have been laggards in using forest carbon to reduce EU emissions. The European Green Deal aims to change this. As part of its long-term emissions reductions, the EU aims to offset this by creating land-based carbon sinks, especially forest carbon sinks as well as carbon capture and storage. This chapter focuses on the role of forest carbon as part of the EU's climate policies towards achieving net-zero greenhouse gas emissions by 2050. It furthermore examines the European Commission's proposed forest strategy and its proposal for a revised LULUCF Regulation. The chapter shows that the logic of appropriateness dominates the European Commission's forest policies. Finally, the chapter makes policy recommendations on how the EU could credibly use long-term carbon sinks to achieve climate neutrality.