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Brodeur, Abel ; Mikola, Derek ; Cook, Nikolai ; Brailey, Thomas ; Briggs, Ryan ; Gendre, Alexandra de ; Dupraz, Yannick ; Fiala, Lenka ; Gabani, Jacopo ; Gauriot, Romain ; Haddad, Joanne ; Lima, Goncalo ; Ankel-Peters, Jörg ; Dreber, Anna ; Campbell, Douglas ; Kattan, Lamis ; Fages, Diego Marino ; Mierisch, Fabian ; Sun, Pu ; Wright, Taylor ; Connolly, Marie ; Hoces de la Guardia, Fernando ; Johannesson, Magnus ; Miguel, Edward ; Vilhuber, Lars ; Abarca, Alejandro ; Acharya, Mahesh ; Adjisse, Sossou Simplice ; Akhtar, Ahwaz ; Lizardi, Eduardo Alberto Ramirez ; Albrecht, Sabina ; Andersen, Synve Nygaard ; Andlib, Zubaria ; Arrora, Falak ; Ash, Thomas ; Bacher, Etienne ; Bachler, Sebastian ; Bacon, Félix ; Bagues, Manuel ; Balogh, Timea ; Batmanov, Alisher ; Barschkett, Mara ; Basdil, B. Kaan ; Dower, Jaromneda ; Castek, Ondrej ; Caviglia-Harris, Jill ; Strand, Gabriella Chauca ; Chen, Shi ; Chzhen, Asya ; Chung, Jong ; Collins, Jason ; Coppock, Alexander ; Cordeau, Hugo ; Couillard, Ben ; Crechet, Jonathan ; Crippa, Lorenzo ; Cui, Jeanne ; Czymara, Christian ; Daarstad, Haley ; Dao, Danh Chi ; Dao, Dong ; Schmandt, Marco David ; Linde, Astrid de ; Melo, Lucas De ; Deer, Lachlan ; Vera, Micole De ; Dimitrova, Velichka ; Dollbaum, Jan Fabian ; Dollbaum, Jan Matti ; Donnelly, Michael ; Huynh, Luu Duc Toan ; Dumbalska, Tsvetomira ; Duncan, Jamie ; Duong, Kiet Tuan ; Duprey, Thibaut ; Dworschak, Christoph ; Ellingsrud, Sigmund ; Elminejad, Ali ; Eissa, Yasmine ; Erhart, Andrea ; Etingin-Frati, Giulian ; Fatemi-Pour, Elaheh ; Federice, Alexa ; Feld, Jan ; Fenig, Guidon ; Firouzjaeiangalougah, Mojtaba ; Fleisje, Erlend ; Fortier-Chouinard, Alexandre ; Engel, Julia Francesca ; Fries, Tilman ; Fortier, Reid ; Fréchet, Nadjim ; Galipeau, Thomas ; Gallegos, Sebastián ; Gangji, Areez ; Gao, Xiaoying ; Garnache, Cloé ; Gáspár, Attila ; Gavrilova, Evelina ; Ghosh, Arijit ; Gibney, Garreth ; Gibson, Grant ; Godager, Geir ; Goff, Leonard ; Gong, Da ; González, Javier ; Gretton, Jeremy ; Griffa, Cristina ; Grigoryeva, Idaliya ; Grtting, Maja ; Guntermann, Eric ; Guo, Jiaqi ; Gugushvili, Alexi ; Habibnia, Hooman ; Häffner, Sonja ; Hall, Jonathan D. ; Hammar, Olle ; Kordt, Amund Hanson ; Hashimoto, Barry ; Hartley, Jonathan S. ; Hausladen, Carina I. ; Havránek, Tomáš ; Hazen, Jacob ; He, Harry ; Hepplewhite, Matthew ; Herrera-Rodriguez, Mario ; Heuer, Felix ; Heyes, Anthony ; Ho, Anson T. Y. ; Holmes, Jonathan ; Holzknecht, Armando ; Hsu, Yu-Hsiang Dexter ; Hu, Shiang-Hung ; Huang, Yu-Shiuan ; Huebener, Mathias ; Huber, Christoph ; Huynh, Kim P. ; Irsova, Zuzana ; Isler, Ozan ; Jakobsson, Niklas ; Frith, Michael James ; Jananji, Raphaël ; Jayalath, Tharaka A. ; Jetter, Michael ; John, Jenny ; Forshaw, Rachel Joy ; Juan, Felipe ; Kadriu, Valon ; Karim, Sunny ; Kelly, Edmund ; Dang, Duy Khanh Hoang ; Khushboo, Tazia ; Kim, Jin ; Kjellsson, Gustav ; Kjelsrud, Anders ; Kotsadam, Andreas ; Korpershoek, Jori ; Krashinsky, Lewis ; Kundu, Suranjana ; Kustov, Alexander ; Lalayev, Nurlan ; Langlois, Audrée ; Laufer, Jill ; Lee-Whiting, Blake ; Leibing, Andreas ; Lenz, Gabriel ; Levin, Joel ; Li, Peng ; Li, Tongzhe ; Lin, Yuchen ; Listo, Ariel ; Liu, Dan ; Lu, Xuewen ; Lukmanova, Elvina ; Luscombe, Alex ; Lusher, Lester R. ; Lyu, Ke ; Ma, Hai ; Mäder, Nicolas ; Makate, Clifton ; Malmberg, Alice ; Maitra, Adit ; Mandas, Marco ; Marcus, Jan ; Margaryan, Shushanik ; Márk, Lili ; Martignano, Andres ; Marsh, Abigail ; Masetto, Isabella ; McCanny, Anthony ; McManus, Emma ; McWay, Ryan ; Metson, Lennard ; Kinge, Jonas Minet ; Mishra, Sumit ; Mohnen, Myra ; Möller, Jakob ; Montambeault, Rosalie ; Montpetit, Sébastien ; Morin, Louis-Philippe ; Morris, Todd ; Moser, Scott ; Motoki, Fabio ; Muehlenbachs, Lucija ; Musulan, Andreea ; Musumeci, Marco ; Nabin, Munirul ; Nchare, Karim ; Neubauer, Florian ; Nguyen, Quan M. P. ; Nguyen, Tuan ; Nguyen-Tien, Viet ; Niazi, Ali ; Nikolaishvili, Giorgi ; Nordstrom, Ardyn ; Nü, Patrick ; Odermatt, Angela ; Olson, Matt ; ien, Henning ; Ölkers, Tim ; Vert, Miquel Oliver i. ; Oral, Emre ; Oswald, Christian ; Ousman, Ali ; Özak, Ömer ; Pandey, Shubham ; Pavlov, Alexandre ; Pelli, Martino ; Penheiro, Romeo ; Park, RyuGyung ; Martel, Eva Pérez ; Petrovičová, Tereza ; Phan, Linh ; Prettyman, Alexa ; Procházka, Jakub ; Putri, Aqila ; Quandt, Julian ; Qiu, Kangyu ; Nguyen, Loan Quynh Thi ; Rahman, Andaleeb ; Rea, Carson H. ; Reiremo, Adam ; Renée, Laëtitia ; Richardson, Joseph ; Rivers, Nicholas ; Rodrigues, Bruno ; Roelofs, William ; Roemer, Tobias ; Rogeberg, Ole ; Rose, Julian ; Roskos-Ewoldsen, Andrew ; Rosmer, Paul ; Sabada, Barbara ; Saberian, Soodeh ; Salamanca, Nicolas ; Sator, Georg ; Sawyer, Antoine ; Scates, Daniel ; Schlüter, Elmar ; Sells, Cameron ; Sen, Sharmi ; Sethi, Ritika ; Shcherbiak, Anna ; Sogaolu, Moyosore ; Soosalu, Matt ; Srensen, Erik ; Sovani, Manali ; Spencer, Noah ; Staubli, Stefan ; Stans, Renske ; Stewart, Anya ; Stips, Felix ; Stockley, Kieran ; Strobel, Stephenson ; Struby, Ethan ; Tang, John ; Tanrisever, Idil ; Yang, Thomas Tao ; Tastan, Ipek ; Tatić, Dejan ; Tatlow, Benjamin ; Seuyong, Féraud Tchuisseu ; Thériault, Rémi ; Thivierge, Vincent ; Tian, Wenjie ; Toma, Filip-Mihai ; Totarelli, Maddalena ; Tran, Van-Anh ; Truong, Hung ; Tsoy, Nikita ; Tuzcuoglu, Kerem ; Ubfal, Diego ; Villalobos, Laura ; Walterskirchen, Julian ; Wang, Joseph Taoyi ; Wattal, Vasudha ; Webb, Matthew D. ; Weber, Bryan ; Weisser, Reinhard ; Weng, Wei-Chien ; Westheide, Christian ; White, Kimberly ; Winter, Jacob ; Wochner, Timo ; Woerman, Matt ; Wong, Jared ; Woodard, Ritchie ; Wroński, Marcin ; Yazbeck, Myra ; Yang, Gustav Chung ; Yap, Luther ; Yassin, Kareman ; Ye, Hao ; Yoon, Jin Young ; Yurris, Chris ; Zahra, Tahreen ; Zaneva, Mirela ; Zayat, Aline ; Zhang, Jonathan ; Zhao, Ziwei ; Yaolang, Zhong
This study pushes our understanding of research reliability by reproducing and replicating claims from 110 papers in leading economic and political science journals. The analysis involves computational reproducibility checks and robustness assessments. It reveals several patterns. First, we uncover a high rate of fully computationally reproducible results (over 85%). Second, excluding minor issues like missing packages or broken pathways, we uncover coding errors for about 25% of studies, with some studies containing multiple errors. Third, we test the robustness of the results to 5,511 re-analyses. We find a robustness reproducibility of about 70%. Robustness reproducibility rates are relatively higher for re-analyses that introduce new data and lower for re-analyses that change the sample or the definition of the dependent variable. Fourth, 52% of re-analysis effect size estimates are smaller than the original published estimates and the average statistical significance of a re-analysis is 77% of the original. Lastly, we rely on six teams of researchers working independently to answer eight additional research questions on the determinants of robustness reproducibility. Most teams find a negative relationship between replicators' experience and reproducibility, while finding no relationship between reproducibility and the provision of intermediate or even raw data combined with the necessary cleaning codes.
Read, Betsy A. ; Kegel, Jessica ; Klute, Mary J. ; Kuo, Alan ; Lefebvre, Stephane C. ; Maumus, Florian ; Mayer, Christoph ; Miller, John ; Monier, Adam ; Salamov, Asaf ; Young, Jeremy ; Aguilar, Maria ; Claverie, Jean-Michel ; Frickenhaus, Stephan ; Gonzalez, Karina ; Herman, Emily K. ; Lin, Yao-Cheng ; Napier, Johnathan ; Ogata, Hiroyuki ; Sarno, Analissa F. ; Shmutz, Jeremy ; Schroeder, Declan ; de Vargas, Colomban ; Verret, Frederic ; von Dassow, Peter ; Valentin, Klaus ; Van de Peer, Yves ; Wheeler, Glen ; Dacks, Joel B. ; Delwiche, Charles F. ; Dyhrman, Sonya T. ; Glöckner, Gernot ; John, Uwe ; Richards, Thomas ; Worden, Alexandra Z. ; Zhang, Xiaoyu ; Grigoriev, Igor V. ; Allen, Andrew E. ; Bidle, Kay ; Borodovsky, M. ; Bowler, C. ; Brownlee, Colin ; Cock, J. Mark ; Elias, Marek ; Gladyshev, Vadim N. ; Groth, Marco ; Guda, Chittibabu ; Hadaegh, Ahmad ; Iglesias-Rodriguez, Maria Debora ; Jenkins, J. ; Jones, Bethan M. ; Lawson, Tracy ; Leese, Florian ; Lindquist, Erika ; Lobanov, Alexei ; Lomsadze, Alexandre ; Malik, Shehre-Banoo ; Marsh, Mary E. ; Mackinder, Luke ; Mock, Thomas ; Müller-Röber, Bernd ; Pagarete, Antonio ; Parker, Micaela ; Probert, Ian ; Quesneville, Hadi ; Raines, Christine ; Rensing, Stefan A. ; Riano-Pachon, Diego Mauricio ; Richier, Sophie ; Rokitta, Sebastian ; Shiraiwa, Yoshihiro ; Soanes, Darren M. ; van der Giezen, Mark ; Wahlund, Thomas M. ; Williams, Bryony ; Wilson, Willie ; Wolfe, Gordon ; Wurch, Louie L.
Coccolithophores have influenced the global climate for over 200 million years(1). These marine phytoplankton can account for 20 per cent of total carbon fixation in some systems(2). They form blooms that can occupy hundreds of thousands of square kilometres and are distinguished by their elegantly sculpted calcium carbonate exoskeletons (coccoliths), rendering them visible from space(3). Although coccolithophores export carbon in the form of organic matter and calcite to the sea floor, they also release CO2 in the calcification process. Hence, they have a complex influence on the carbon cycle, driving either CO2 production or uptake, sequestration and export to the deep ocean(4). Here we report the first haptophyte reference genome, from the coccolithophore Emiliania huxleyi strain CCMP1516, and sequences from 13 additional isolates. Our analyses reveal a pan genome (core genes plus genes distributed variably between strains) probably supported by an atypical complement of repetitive sequence in the genome. Comparisons across strains demonstrate that E. huxleyi, which has long been considered a single species, harbours extensive genome variability reflected in different metabolic repertoires. Genome variability within this species complex seems to underpin its capacity both to thrive in habitats ranging from the equator to the subarctic and to form large-scale episodic blooms under a wide variety of environmental conditions.
Overduin, Pier Paul ; Westermann, Sebastian ; Yoshikawa, Kenji ; Haberlau, Thomas ; Romanovsky, Vladimir E. ; Wetterich, Sebastian
Submarine permafrost degradation rates may be determined by a number of interacting processes, including rates of sea level rise and coastal erosion, sea bottom temperature and salinity regimes, geothermal heat flux and heat and mass diffusion within the sediment column. Observations of ice-bearing permafrost in shelf sediments are necessary in order to determine its spatial distribution and to quantify its degradation rate. We tested the use of direct current electrical resistivity to ice-bearing permafrost in Elson Lagoon northeast of Barrow, Alaska (Beaufort Sea). A sharp increase in electrical resistivity was observed in profiles collected perpendicular to and along the coastline and is interpreted to be the boundary between ice-free sediment and underlying ice-bearing submarine permafrost. The depth to the interpreted ice-bearing permafrost increases from <2 m below sea level to over 12 m below sea level with increasing distance from the coastline. The dependence of the saline sediment electrical resistivity on temperature and freezing was measured in the laboratory to provide validation for the field measurements. Electrical resistivity was shown to be effective for detection of shallow ice-bearing permafrost in the coastal zone. Historical coastal retreat rates were combined with the inclination of the top of the ice-bearing permafrost to calculate mean vertical permafrost degradation rates of 1 to 4 cm yr(-1).
Kuhlicke, Christian ; Seebauer, Sebastian ; Hudson, Paul ; Begg, Chloe ; Bubeck, Philip ; Dittmer, Cordula ; Grothmann, Torsten ; Heidenreich, Anna ; Kreibich, Heidi ; Lorenz, Daniel F. ; Masson, Torsten ; Reiter, Jessica ; Thaler, Thomas ; Thieken, Annegret ; Bamberg, Sebastian
Recent policy changes highlight the need for citizens to take adaptive actions to reduce flood-related impacts. Here, we argue that these changes represent a wider behavioral turn in flood risk management (FRM). The behavioral turn is based on three fundamental assumptions: first, that the motivations of citizens to take adaptive actions can be well understood so that these motivations can be targeted in the practice of FRM; second, that private adaptive measures and actions are effective in reducing flood risk; and third, that individuals have the capacities to implement such measures. We assess the extent to which the assumptions can be supported by empirical evidence. We do this by engaging with three intellectual catchments. We turn to research by psychologists and other behavioral scientists which focus on the sociopsychological factors which influence individual motivations (Assumption 1). We engage with economists, engineers, and quantitative risk analysts who explore the extent to which individuals can reduce flood related impacts by quantifying the effectiveness and efficiency of household-level adaptive measures (Assumption 2). We converse with human geographers and sociologists who explore the types of capacities households require to adapt to and cope with threatening events (Assumption 3). We believe that an investigation of the behavioral turn is important because if the outlined assumptions do not hold, there is a risk of creating and strengthening inequalities in FRM. Therefore, we outline the current intellectual and empirical knowledge as well as future research needs. Generally, we argue that more collaboration across intellectual catchments is needed, that future research should be more theoretically grounded and become methodologically more rigorous and at the same time focus more explicitly on the normative underpinnings of the behavioral turn.
Kuhlicke, Christian ; Seebauer, Sebastian ; Hudson, Paul ; Begg, Chloe ; Bubeck, Philip ; Dittmer, Cordula ; Grothmann, Torsten ; Heidenreich, Anna ; Kreibich, Heidi ; Lorenz, Daniel F. ; Masson, Torsten ; Reiter, Jessica ; Thaler, Thomas ; Thieken, Annegret ; Bamberg, Sebastian
Recent policy changes highlight the need for citizens to take adaptive actions to reduce flood-related impacts. Here, we argue that these changes represent a wider behavioral turn in flood risk management (FRM). The behavioral turn is based on three fundamental assumptions: first, that the motivations of citizens to take adaptive actions can be well understood so that these motivations can be targeted in the practice of FRM; second, that private adaptive measures and actions are effective in reducing flood risk; and third, that individuals have the capacities to implement such measures. We assess the extent to which the assumptions can be supported by empirical evidence. We do this by engaging with three intellectual catchments. We turn to research by psychologists and other behavioral scientists which focus on the sociopsychological factors which influence individual motivations (Assumption 1). We engage with economists, engineers, and quantitative risk analysts who explore the extent to which individuals can reduce flood related impacts by quantifying the effectiveness and efficiency of household-level adaptive measures (Assumption 2). We converse with human geographers and sociologists who explore the types of capacities households require to adapt to and cope with threatening events (Assumption 3). We believe that an investigation of the behavioral turn is important because if the outlined assumptions do not hold, there is a risk of creating and strengthening inequalities in FRM. Therefore, we outline the current intellectual and empirical knowledge as well as future research needs. Generally, we argue that more collaboration across intellectual catchments is needed, that future research should be more theoretically grounded and become methodologically more rigorous and at the same time focus more explicitly on the normative underpinnings of the behavioral turn.
Flóvenz, Ólafur G. ; Wang, Rongjiang ; Hersir, Gylfi Páll ; Dahm, Torsten ; Hainzl, Sebastian ; Vassileva, Magdalena ; Drouin, Vincent ; Heimann, Sebastian ; Isken, Marius Paul ; Gudnason, Egill Á. ; Ágústsson, Kristján ; Ágústsdóttir, Thorbjörg ; Horálek, Josef ; Motagh, Mahdi ; Walter, Thomas R. ; Rivalta, Eleonora ; Jousset, Philippe ; Krawczyk, Charlotte M. ; Milkereit, Claus
Understanding and constraining the source of geodetic deformation in volcanic areas is an important component of hazard assessment. Here, we analyse deformation and seismicity for one year before the March 2021 Fagradalsfjall eruption in Iceland. We generate a high-resolution catalogue of 39,500 earthquakes using optical cable recordings and develop a poroelastic model to describe three pre-eruptional uplift and subsidence cycles at the Svartsengi geothermal field, 8 km west of the eruption site. We find the observed deformation is best explained by cyclic intrusions into a permeable aquifer by a fluid injected at 4 km depth below the geothermal field, with a total volume of 0.11 ± 0.05 km3 and a density of 850 ± 350 kg m–3. We therefore suggest that ingression of magmatic CO2 can explain the geodetic, gravity and seismic data, although some contribution of magma cannot be excluded.
Sharkovska, Yuliya ; Reichetzeder, Christoph ; Alter, Markus L. ; Tsuprykov, Oleg ; Bachmann, Sebastian ; Secher, Thomas ; Klein, Thomas ; Hocher, Berthold
Background: Despite the beneficial effects of type 4 dipeptidyl peptidase (DPP-4) inhibitors on glucose levels, its effects on diabetic nephropathy remain unclear.
Method: This study examined the long-term renoprotective effects of DPP-4 inhibitor linagliptin in db/db mice, a model of type 2 diabetes.
Results were compared with the known beneficial effects of renin-angiotensin system blockade by enalapril. Ten-week-old male diabetic db/db mice were treated for 3 months with either vehicle (n = 10), 3 mg linagliptin/kg per day (n = 8), or 20 mg enalapril/kg per day (n = 10). Heterozygous db/m mice treated with vehicle served as healthy controls (n = 8). Results: Neither linagliptin nor enalapril had significant effects on the parameters of glucose metabolism or blood pressure in diabetic db/db mice. However, linagliptin treatment reduced albuminuria and attenuated kidney injury. In addition, expression of podocyte marker podocalyxin was normalized. We also analysed DPP-4 expression by immunofluorescence in human kidney biopsies and detected upregulation of DPP-4 in the glomeruli of patients with diabetic nephropathy, suggesting that our findings might be of relevance for human kidney disease as well.
Conclusion: Treatment with DPP-4 inhibitor linagliptin delays the progression of diabetic nephropathy damage in a glucose-independent and blood-pressure-independent manner. The observed effects may be because of the attenuation of podocyte injury and inhibition of myofibroblast transformation.
Frescatada-Rosa, Marcia ; Stanislas, Thomas ; Backues, Steven K. ; Reichardt, Ilka ; Men, Shuzhen ; Boutte, Yohann ; Juergens, Gerd ; Moritz, Thomas ; Bednarek, Sebastian York ; Grebe, Markus
Membranes of eukaryotic cells contain high lipid-order sterol-rich domains that are thought to mediate temporal and spatial organization of cellular processes. Sterols are crucial for execution of cytokinesis, the last stage of cell division, in diverse eukaryotes. The cell plate of higher-plant cells is the membrane structure that separates daughter cells during somatic cytokinesis. Cell-plate formation in Arabidopsis relies on sterol- and DYNAMIN-RELATED PROTEIN1A (DRP1A)-dependent endocytosis. However, functional relationships between lipid membrane order or lipid packing and endocytic machinery components during eukaryotic cytokinesis have not been elucidated. Using ratiometric live imaging of lipid order-sensitive fluorescent probes, we show that the cell plate of Arabidopsis thaliana represents a dynamic, high lipid-order membrane domain. The cell-plate lipid order was found to be sensitive to pharmacological and genetic alterations of sterol composition. Sterols co-localize with DRP1A at the cell plate, and DRP1A accumulates in detergent-resistant membrane fractions. Modifications of sterol concentration or composition reduce cell-plate membrane order and affect DRP1A localization. Strikingly, DRP1A function itself is essential for high lipid order at the cell plate. Our findings provide evidence that the cell plate represents a high lipid-order domain, and pave the way to explore potential feedback between lipid order and function of dynamin-related proteins during cytokinesis.
Staubitz, Thomas ; Serth, Sebastian ; Thomas, Max ; Ebner, Martin ; Koschutnig-Ebner, Markus ; Rampelt, Florian ; von Stetten, Alexander ; Wittke, Andreas
The MOOChub is a joined web-based catalog of all relevant German and Austrian MOOC platforms that lists well over 750 Massive Open Online Courses (MOOCs). Automatically building such a catalog requires that all partners describe and publicly offer the metadata of their courses in the same way. The paper at hand presents the genesis of the idea to establish a common metadata standard and the story of its subsequent development. The result of this effort is, first, an open-licensed de-facto-standard, which is based on existing commonly used standards and second, a first prototypical platform that is using this standard: the MOOChub, which lists all courses of the involved partners. This catalog is searchable and provides a more comprehensive overview of basically all MOOCs that are offered by German and Austrian MOOC platforms. Finally, the upcoming developments to further optimize the catalog and the metadata standard are reported.