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The haemolymph of the adult Colorado potato beetle, Lepinotarsa decemlineata Say, contains a high molecular weight (MW > 200,000) JH-III specific binding protein. The Kd value of the protein for racemic JH-III is 1.3 ± 0.2 × 10−7 M. It has a lower affinity for racemic JH-I and it does not bind JH-III-diol or JH-III-acid. The binding protein does discriminate between the enantiomers of synthetic, racemic JH-III as was determined by stereochemical anaysis of the bound and the free JH-III. Incubation of racemic JH-III with crude haemolymph results in preferential formation of (10S)-JH-III-acid, the unnatural configuration. The JH-esterase present in L. decemlineata haemolymph is not enantioselective. It is concluded that the most important function of the binding protein is that of a specific carrier, protecting the natural hormone against degradation by esterases. The carrier does not protect JH-I as efficiently as the lower homologue.
We discuss our most recent findings on the diffuse X-ray emission within Wolf-Rayet (WR) nebulae. The best-quality X-ray observations of these objects are those performed by XMM- Newton and Chandra towards S 308, NGC 2359, and NGC 6888. Even though these three WR nebulae might have different formation scenarios, they all share similar characteristics: i) the main plasma temperatures of the X-ray-emitting gas is found to be T =[1–2]×^K, ii) the diffuse X-ray emission is confined inside the [O iii] shell, and iii) their X-ray luminosities and electron densities in the 0.3–2.0 keV energy range are LX ≈10^33–10^34 erg s-1 and ne ≈0.1–1 cm^-3 . These properties and the nebular-like abundances of the hot gas suggest mixing and/or thermal conduction is taking an important rôle reducing the temperature of the hot bubble.
Two types of X-ray sources are mostly found in planetary nebulae (PNe): point sources at their central stars and diffuse emission inside hot bubbles. Here we describe these two types of sources based on the most recent observations obtained in the framework of the Chandra Planetary Nebula Survey, ChanPlaNS, an X-ray survey targeting a volume-limited sample of PNe. Diffuse X-ray emission is found preferentially in young PNe with sharp, closed inner
shells. Point sources of X-ray emission at the central stars reveal magnetically active binary companions and shock-in stellar winds.