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- Militär & ländliche Gesellschaft in der FNZ Kolloquium in Rostock (Programm) - Tagung der AG- FNZ, Essen (16.-18. September 1999) - Veröffentlichungen der AMG in der preußischen Armee(B. Käser) - „Vom Untertan zum Staatsbürger...“ (M. Plassmann) - Informationen zum Museum in Wittstock - Absolutistischer Staat und Heeresordnung. (Dr. M. Busch - „Kriegserfahrung und Religion ...1792-1815“ (PD Dr. H. Carl) - „Vom Partheygänger“ zum Partisann (M. Rink) - „Cuius exercitus, eius religio?“ (Dr. M. Kaiser) - „Mars mit Zopf? Aufstieg und Fall des Ernst v. Rüchel“ (1754-1823) (O. Jessen) - AMG-Sitzung auf dem Historikertag in Frankfurt a. M. - Beitrittserklärung
Aus dem Inhalt dieser Ausgabe: BEITRÄGE: Peter H. Wilson: British and american perspectives on early modern warfare Mikko Huhtamies: Kriegswesen und Gesellschaft in der frühen Neuzeit in der finnisch-schwedischen Geschichtsforschung INTERNET: Stephan Huck: Nutzungsmöglichkeiten des Internets Gundula Gahlen , Michael Herrmann, Torsten F. Reimer und Norbert Winnige: Militärgeschichte der Frühen Neuzeit im Internet Die Online-Angebote des AMG und sfn Markus Pöhlmann: Die Internetpräsenz des Arbeitskreises Militärgeschichte e.V. Josef Pauser: Das Online-Angebot des Arbeitskreises .Policey/Polizei im vormodernen Europa. Torsten F. Reimer: Eine Studie in Kriegführung. Eine Vorstellung des H-War Military History Network PROJEKTE: Antje Fuchs: Ein neuer Konfessionskrieg? Erfahrungen von Krieg und Okkupation im Kurfürstentum Hannover und benachbarten geistlichen Fürstentümern zur Zeit des Siebenjährigen Krieges 1756-1763 Sascha Möbius: Ergebnisse der Magisterarbeit .Psychologische Aspekte friederizianischer Taktik im Siebenjährigen Krieg. Wolfgang Heil: Die Gemeinen Soldaten. Das Sozialleben der militärischen Unterschicht im altpreußischen Heer und seine Stellung in der altständischen Gesellschaft. Rainer Jacobs: Militärische Dienstpflichten in der Frühen Neuzeit Michael Kaiser und Stefan Kroll: Forschungsprojekt Militär und Religiosität in der Frühen Neuzeit Ralf Blank: Die Schlosskanonen von Hohenlimburg Artilleriegeschütze mit einer wechselvollen Geschichte Gundula Gahlen und Olaf Gründel: Kataster zur Schlacht bei Wittstock von 1636 Jan Schlürmann: Die Schleswig-Holstein-Gottorfischen Auxiliairtruppen im Spanischen Erbfolgekrieg . Ergebnisse einer Untersuchung BERICHTE: Renko Geffarth: Die besetzte res publica. Zum Verhältnis von ziviler Obrigkeit und militärischer Herrschaft in besetzten Gebieten vom Spätmittelalter bis zum 18. Jahrhundert. Gundula Bavendamm: Operationsgeschichte und moderne Historiographie. Ein Widerspruch?
Myriapods (e. g., centipedes and millipedes) display a simple homonomous body plan relative to other arthropods. All members of the class are terrestrial, but they attained terrestriality independently of insects. Myriapoda is the only arthropod class not represented by a sequenced genome. We present an analysis of the genome of the centipede Strigamia maritima. It retains a compact genome that has undergone less gene loss and shuffling than previously sequenced arthropods, and many orthologues of genes conserved from the bilaterian ancestor that have been lost in insects. Our analysis locates many genes in conserved macro-synteny contexts, and many small-scale examples of gene clustering. We describe several examples where S. maritima shows different solutions from insects to similar problems. The insect olfactory receptor gene family is absent from S. maritima, and olfaction in air is likely effected by expansion of other receptor gene families. For some genes S. maritima has evolved paralogues to generate coding sequence diversity, where insects use alternate splicing. This is most striking for the Dscam gene, which in Drosophila generates more than 100,000 alternate splice forms, but in S. maritima is encoded by over 100 paralogues. We see an intriguing linkage between the absence of any known photosensory proteins in a blind organism and the additional absence of canonical circadian clock genes. The phylogenetic position of myriapods allows us to identify where in arthropod phylogeny several particular molecular mechanisms and traits emerged. For example, we conclude that juvenile hormone signalling evolved with the emergence of the exoskeleton in the arthropods and that RR-1 containing cuticle proteins evolved in the lineage leading to Mandibulata. We also identify when various gene expansions and losses occurred. The genome of S. maritima offers us a unique glimpse into the ancestral arthropod genome, while also displaying many adaptations to its specific life history.
L-edge spectroscopy of 3d transition metals provides important electronic structure information and has been used in many fields. However, the use of this method for studying dilute aqueous systems, such as metalloenzymes, has not been prevalent because of severe radiation damage and the lack of suitable detection systems. Here we present spectra from a dilute Mn aqueous solution using a high-transmission zone-plate spectrometer at the Linac Coherent Light Source (LCLS). The spectrometer has been optimized for discriminating the Mn L-edge signal from the overwhelming 0 K-edge background that arises from water and protein itself, and the ultrashort LCLS X-ray pulses can outrun X-ray induced damage. We show that the deviations of the partial-fluorescence yield-detected spectra from the true absorption can be well modeled using the state-dependence of the fluorescence yield, and discuss implications for the application of our concept to biological samples.
BAIER, D.; SOYEZ, K.: Dekontamination von Altholz ; BAIER, D. et al.: Untersuchungen zur Kompostierbarkeit von paraffinbeschichteten Verpackungsmaterialien ; KAISER, J.; SOYEZ, K.: Zum Wasser- und Wärmehaushalt des Intensivrotteprozesses der mechanisch-biologischen Abfallbehandlung ; KAMM, B. et al.: Green Biorefinery - European Network for the Implementation of Biorefineries (NIB) ; KAMM, B. et al.: Grüne Bioraffinerie Brandenburg ; KOLLER, M.; HERMANN, T.: Entscheidungshilfen für die ökologische Optimierung der Entsorgung häuslicher Restabfälle ; SCHMEER, E.: Solarthermie 2000 ; SCHMEER, E.: Forschungs- und Demonstrationsanlage zur Photovoltaik ; SOYEZ, K. et al.: Verbundvorhaben "Mechanisch-biologische Restabfallbehandlung" ; STARKE, I. et al.: Carbohydrates as raw material from a Green BioRefinery
This study aimed to build on the relationship of well-established self-report and behavioral assessments to the latent constructs positive (PVS) and negative valence systems (NVS), cognitive systems (CS), and social processes (SP) of the Research Domain Criteria (RDoC) framework in a large transnosological population which cuts across DSM/ICD-10 disorder criteria categories. One thousand four hundred and thirty one participants (42.1% suffering from anxiety/fear-related, 18.2% from depressive, 7.9% from schizophrenia spectrum, 7.5% from bipolar, 3.4% from autism spectrum, 2.2% from other disorders, 18.4% healthy controls, and 0.2% with no diagnosis specified) recruited in studies within the German research network for mental disorders for the Phenotypic, Diagnostic and Clinical Domain Assessment Network Germany (PD-CAN) were examined with a Mini-RDoC-Assessment including behavioral and self-report measures. The respective data was analyzed with confirmatory factor analysis (CFA) to delineate the underlying latent RDoC-structure. A revised four-factor model reflecting the core domains positive and negative valence systems as well as cognitive systems and social processes showed a good fit across this sample and showed significantly better fit compared to a one factor solution. The connections between the domains PVS, NVS and SP could be substantiated, indicating a universal latent structure spanning across known nosological entities. This study is the first to give an impression on the latent structure and intercorrelations between four core Research Domain Criteria in a transnosological sample. We emphasize the possibility of using already existing and well validated self-report and behavioral measurements to capture aspects of the latent structure informed by the RDoC matrix.
Separation of coarse organic particles from bulk surface soil samples by electrostatic attraction
(2009)
Different separation procedures are suggested for studying the stability and functionality of sod organic matter (OM). Density fractionation procedures using high-molarity, water-based salt solutions to separate organic particles may cause losses or transfers of C between particle and soluble OM fractions during separation, which may be a result of solution processes. The objective of this study was to separate coarse organic particles (>0.315 mm) from air- dried surface soil samples to avoid such solution processes as far as possible. Air-dried surface soil samples (<2 mm) from nine adjacent arable and forest sites were sieved into five soil particle size fractions (2-1.25, 1.25-0.8, 0.8- 0.5, 0.5-0.4, and 0.4-0.315 mm). Coarse organic particles were separated from each of these fractions using electrostatic attraction by a charged glass surface. The sum of the total dry matter content of the electrostatically separated coarse organic particles ranged from 0.05 to 140 g kg(-1). Scanning electron microscopy images and organic C (OC) analyses indicated, however, that the coarse organic particle fractions were also composed of 20 to 76% mineral particles (i.e., 200-760 g mineral kg(-1) fraction). The repeatability of the electrostatic attraction procedure falls within a range similar to that of accepted density fractionation methods using high-molarity salt solutions. Based on the similarity in repeatability, we suggest that the electrostatic attraction procedure will successfully remove coarse organic particles (>0.315 mm) from air-dried surface soil samples. Because aqueous solutions are not used, the electrostatic attraction procedure to separate coarse organic particles avoids C losses and transfers associated with solution-dependent techniques. Therefore, this method can be used as a pretreatment for subsequent density- or solubility-based soil OM fractionation procedures.
Questions remain about the exact ultrasonic energy level that is required to effectively disperse soil aggregates and to what extent this is accompanied by physical damage to individual soil particles. We found maximum aggregate dispersion at energy levels of 1500 J?cm3 and no evidence for the disintegration of particles < 20 mu m even at that energy level. Our findings suggest that sonication at energies much greater than those applied conventionally can disperse aggregates of high mechanical stability.
Identifying the chemical mechanisms behind soil carbon bound in organo-mineral complexes is necessary to determine the degree to which soil organic carbon is stabilized belowground. Analysis of delta C-13 and delta N-15 isotopic signatures of stabilized OM fractions along with soil mineral characteristics may yield important information about OM-mineral associations and their processing history. We anlayzed the delta C-13 and delta N-15 isotopic signatures from two organic matter (OM) fractions along with soil mineral proxies to identify the likely binding mechanisms involved. We analyzed OM fractions hypothesized to contain carbon stabilized through organo-mineral complexes: (1) OM separated chemically with sodium pyrophosphate (OM(PY)) and (2) OM occluded in micro-structures found in the chemical extraction residue (OM(ER)). Because the OM fractions were separated from five different soils with paired forest and arable land use histories, we could address the impact of land use change on carbon binding and processing mechanisms. We used partial least squares regression to analyze patterns in the isotopic signature of OM with established mineral and chemical proxies indicative for certain binding mechanisms. We found different mechanisms predominate in each land use type. For arable soils, the formation of OM(PY)-Ca-mineral associations was identified as an important OM binding mechanism. Therefore, we hypothesize an increased stabilization of microbial processed OM(PY) through Ca2+ interactions. In general, we found the forest soils to contain on average 10% more stabilized carbon relative to total carbon stocks, than the agricultural counter part. In forest soils, we found a positive relationship between isotopic signatures of OM(PY) and the ratio of soil organic carbon content to soil surface area (SOC/SSA). This indicates that the OM(PY) fractions of forest soils represent layers of slower exchange not directly attached to mineral surfaces. From the isotopic composition of the OM(ER) fraction, we conclude that the OM in this fraction from both land use types have undergone a different pathway to stabilization that does not involve microbial processing, which may include OM which is highly protected within soil micro-structures.
Land use and mineral characteristics affect the ability of surface as well as subsurface soils to sequester organic carbon and their contribution to mitigation of the greenhouse effect. There is less information about the effects of land use and soil properties on the amount and composition of organic matter (OM) for subsurface soils as compared with surface soils. Here we aimed to analyse the long-term (>= 100 years) impact of arable and forest land use and soil mineral characteristics on subsurface soil organic carbon (SOC) contents, as well as on amount and composition of OM sequentially separated by Na pyrophosphate solution (OM(PY)) from subsurface soil samples. Seven soils with different mineral characteristics (Albic and Haplic Luvisol, Colluvic and Haplic Regosol, Haplic and Vertic Cambisol, Haplic Stagnosol) were selected from within Germany. Soil samples were taken from subsurface horizons of forest and adjacent arable sites continuously used for > 100 years. The OM(PY) fractions were analysed for their OC content (OC(PY)) and characterized by Fourier transform infrared spectroscopy. Multiple regression analyses for the arable subsurface soils indicated significant positive relationships between the SOC contents and combined effects of the (i) exchangeable Ca (Ca(ex)) and oxalate-soluble Fe (Fe(ox)) and (ii) the Ca(ex) and Al(ox) contents. For these soils the increase in OC (OC(PY) multiplied by the relative C=O content of OM(PY)) and increasing contents of Ca(ex) indicated that OM(PY) mainly interacts with Ca2+. For the forest subsurface soils (pH < 5), the OC(PY) contents were related to the contents of Na-pyrophosphate-soluble Fe and Al. The long-term arable and forest land use seems to result in different OM(PY)-mineral interactions in subsurface soils. On the basis of this, we hypothesize that a long-term land-use change from arable to forest may lead to a shift from mainly OM(PY)-Ca2+ to mainly OM(PY)-Fe3+ and -Al3+ interactions if the pH of subsurface soils significantly decreases to < 5.
Mineral topsoils possess large organic carbon (OC) contents but there is only limited knowledge on the mechanisms controlling the preservation of organic matter (OM) against microbial decay. Samples were taken from the uppermost mineral topsoil horizon (0 to 5 cm) of seven sites under mature deciduous forest showing OC contents between 69 and 164 g kg(-1) and a wide range in mineral characteristics. At first, organic particles and the water-extractable OM were removed from the soil samples. Thereafter, Na-pyrophosphate extractable organic matter (OM(PY)), assumed to be indicative for OM bound via cation mediated interactions, and the OM remaining in the extraction residue (OM(ER)), supposed to be indicative for OM occluded in mechanically highly stable micro-aggregates, were sequentially separated and quantified. The composition of OM(PY) and OM(ER) was analyzed by FTIR and their stability by C-14 measurements. The OC remaining in the extraction residues accounted for 38 to 59% of the bulk soil OC (SOC) suggesting a much larger relevance of OM(ER) for the OM dynamic in the analyzed soils as compared with OM(PY) that accounted for 1.6 to 7.5% of the SOC. The FUR analyses revealed a lower relative proportion of C=O groups in OM(ER) compared to OM(PY) indicating differences in the degree of microbial processing between these fractions. Correlation analyses suggest an increase in the stability of OM(PY) with the soil pH and contents of Na-pyrophosphate soluble Fe, Al, and Mg and an increase in the stability of OM(ER) with the soil pH and the contents of clay and oxalate-soluble Fe and Al. Despite the detected influence of soil mineral characteristics on the turnover of OM(PY) and OM(ER), the Delta C-14 signatures indicated mean residence times less than 100 years. The presence of less stabilized OM in these fractions can be derived from methodological uncertainties and/or the fast cycling compartment of mineral-associated OM. (C) 2015 Elsevier B.V. All rights reserved.
A submerged pine forest from the early Holocene in the Mecklenburg Lake District, northern Germany
(2018)
For the first time, evidence of a submerged pine forest from the early Holocene can be documented in a central European lake. Subaquatic tree stumps were discovered in Lake Giesenschlagsee at a depth of between 2 and 5m using scuba divers, side-scan sonar and a remotely operated vehicle. Several erect stumps, anchored to the ground by roots, represent an insitu record of this former forest. Botanical determination revealed the stumps to be Scots pine (Pinus sylvestris) with an individual tree age of about 80years. The trees could not be dated by means of dendrochronology, as they are older than the regional reference chronology for pine. Radiocarbon ages from the wood range from 10880 +/- 210 to 10370 +/- 130cal. a BP, which is equivalent to the mid-Preboreal to early Boreal biozones. The trees are rooted in sedge peat, which can be dated to this period as well, using pollen stratigraphical analysis. Tilting of the peat bed by 4m indicates subsidence of the ground due to local dead ice melting, causing the trees to become submerged and preserved for millennia. Together with recently detected Lateglacial insitu tree occurrences in nearby lakes, the submerged pine forest at Giesenschlagsee represents a new and highly promising type of geo-bio-archive for the wider region. Comparable insitu pine remnants occur at some terrestrial (buried setting) and marine (submerged setting) sites in northern central Europe and beyond, but they partly differ in age. In general, the insitu pine finds document shifts of the zonal boreal forest ecosystem during the late Quaternary.
Ziel dieser Arbeit ist es, ein sequentielles Extraktionsverfahren zur Erfassung unterschiedlich stabiler Anteile der orgnischen Bodensubstanz (OBS) zu entwickeln und zu klären, ob ein Zusammenhang zwischen Löslichkeit und Stabilität besteht. Darüber hinaus sollen der Einfluss von Bewirtschaftungsmaßnahmen auf Menge und Zusammensetzung dieser OBS-Anteile und Zusammenhänge zwischen Fourier Transform Infrarot (FT-IR)-Spektroskopiedaten und der Kationenaustauschkapazität (KAK) der OBS analysiert werden. Für die Untersuchungen wurden Böden der Langzeitfeldexperimente (LFE) in Halle, Bad Lauchstädt und Rotthalmünster beprobt. Zur Erfassung unterschiedlicher OBS-Fraktionen wurden im ersten Schritt die wasserlöslichen OBS-Anteile (OBS(W)-Fraktion) aus den Böden isoliert. Im zweiten Schritt wurden aus den Extraktionsrückständen der Wasserextraktion OBS-Anteile mit einer Natrium (Na)-Pyrophosphatlösung extrahiert (OBS(PY)-Fraktion). Die Stabilität der OBS-Fraktionen wurde anhand von δ13C-Bestimmungen und 14C-Messungen untersucht. Die Charakterisierung der Zusammensetzung der OBS-Fraktionen erfolgte mittels FT-IR Spektroskopie. Generell wird mit der OBS(PY)-Fraktion ein größerer Anteil am organischen Kohlenstoffgehalt der Böden erfasst als mit der OBS(W)-Fraktion. Die δ13C- und 14C-Daten zeigen, dass die OBS(W)-Fraktion höhere Anteile jungen organischen Materials als die OBS(PY)-Fraktion enthält. Das entwickelte sequentielle Extraktionsverfahren ist also prinzipiell geeignet unterschiedlich stabile OBS-Anteile anhand ihrer Löslichkeit zu isolieren. Mittels FT-IR spektroskopischer Untersuchungen wird festgestellt, dass Bewirtschaftungsmaßnahmen, wie die Düngung, sowie Standorteigenschaften die Zusammensetzung der OBS-Fraktionen beeinflussen. Für die OBS(PY)-Fraktion ist dies stärker ausgeprägt als für die OBS(W)-Fraktion. Die KAK der OBS(PY)-Fraktion aus den Böden der LFE in Halle und Bad Lauchstädt ist positiv mit der Absorptionsintensität der C=O-Bande in den FT-IR Spektren dieser OBS-Fraktion korreliert.
Intensive land use is a major cause of biodiversity loss, but most studies comparing the response of multiple taxa rely on simple diversity measures while analyses of other community attributes are only recently gaining attention. Species-abundance distributions (SADs) are a community attribute that can be used to study changes in the overall abundance structure of species groups, and whether these changes are driven by abundant or rare species. We evaluated the effect of grassland management intensity for three land-use modes (fertilization, mowing, grazing) and their combination on species richness and SADs for three belowground (arbuscular mycorrhizal fungi, prokaryotes and insect larvae) and seven aboveground groups (vascular plants, bryophytes and lichens; arthropod herbivores; arthropod pollinators; bats and birds). Three descriptors of SADs were evaluated: general shape (abundance decay rate), proportion of rare species (rarity) and proportional abundance of the commonest species (dominance). Across groups, taxonomic richness was largely unaffected by land-use intensity and only decreased with increasing mowing intensity. Of the three SAD descriptors, abundance decay rate became steeper with increasing combined land-use intensity across groups. This reflected a decrease in rarity among plants, herbivores and vertebrates. Effects of fertilization on the three descriptors were similar to the combined land-use intensity effects. Mowing intensity only affected the SAD descriptors of insect larvae and vertebrates, while grazing intensity produced a range of effects on different descriptors in distinct groups. Overall, belowground groups had more even abundance distribtitions than aboveground groups. Strong differences among aboveground groups and between above- and belowground groups indicate that no single taxonomic group can serve as an indicator for effects in other groups. In the past, the use of SADs has been hampered by concerns over theoretical models underlying specific forms of SADs. Our study shows that SAD descriptors that are not connected to a particular model are suitable to assess the effect of land use on community structure.
X-ray absorption spectroscopy at the L-edge of 3d transition metals provides unique information on the local metal charge and spin states by directly probing 3d-derived molecular orbitals through 2p-3d transitions. However, this soft x-ray technique has been rarely used at synchrotron facilities for mechanistic studies of metalloenzymes due to the difficulties of x-ray-induced sample damage and strong background signals from light elements that can dominate the low metal signal. Here, we combine femtosecond soft x-ray pulses from a free-electron laser with a novel x-ray fluorescence-yield spectrometer to overcome these difficulties. We present L-edge absorption spectra of inorganic high-valent Mn complexes (Mn similar to 6-15 mmol/l) with no visible effects of radiation damage. We also present the first L-edge absorption spectra of the oxygen evolving complex (Mn4CaO5) in Photosystem II (Mn < 1 mmol/l) at room temperature, measured under similar conditions. Our approach opens new ways to study metalloenzymes under functional conditions. (C) 2017 Author(s).
Reflexion ist eine Schlüsselkategorie für die professionelle Entwicklung von Lehrkräften, welche als Ausbildungsziel in den Bildungsstandards für die Lehrkräftebildung verankert ist. Eine Verstetigung universitär geprägter Forschung und Modellierung in der praxisnahen Anwendung im schulischen Kontext bietet Potentiale nachhaltiger Professionalisierung. Die Stärkung reflexionsbezogener Kompetenzen durch Empirie und Anwendung scheint eine phasenübergreifende Herausforderung der Lehrkräftebildung zu sein, die es zu bewältigen gilt. Ziele des Tagungsbandes Reflexion in der Lehrkräftebildung sind eine theoretische Schärfung des Konzeptes „Reflexive Professionalisierung“ und der Austausch über Fragen der Einbettung wirksamer reflexionsbezogener Lerngelegenheiten in die Lehrkräftebildung. Forschende und Lehrende der‚ drei Phasen (Studium, Referendariat sowie Fort- und Weiterbildung) der Lehrkräftebildung stellen Lehrkonzepte und Forschungsprojekte zum Thema Reflexion in der Lehrkräftebildung vor und diskutieren diese. Gemeinsam mit Teilnehmenden aller Phasen und von verschiedenen Standorten der Lehrkräftebildung werden zukünftige Herausforderungen identifiziert und Lösungsansätze herausgearbeitet.
MALDI-TOF-MS-based identification of monoclonal murine Anti-SARS-CoV-2 antibodies within one hour
(2022)
During the SARS-CoV-2 pandemic, many virus-binding monoclonal antibodies have been developed for clinical and diagnostic purposes. This underlines the importance of antibodies as universal bioanalytical reagents. However, little attention is given to the reproducibility crisis that scientific studies are still facing to date. In a recent study, not even half of all research antibodies mentioned in publications could be identified at all. This should spark more efforts in the search for practical solutions for the traceability of antibodies. For this purpose, we used 35 monoclonal antibodies against SARS-CoV-2 to demonstrate how sequence-independent antibody identification can be achieved by simple means applied to the protein. First, we examined the intact and light chain masses of the antibodies relative to the reference material NIST-mAb 8671. Already half of the antibodies could be identified based solely on these two parameters. In addition, we developed two complementary peptide mass fingerprinting methods with MALDI-TOF-MS that can be performed in 60 min and had a combined sequence coverage of over 80%. One method is based on the partial acidic hydrolysis of the protein by 5 mM of sulfuric acid at 99 degrees C. Furthermore, we established a fast way for a tryptic digest without an alkylation step. We were able to show that the distinction of clones is possible simply by a brief visual comparison of the mass spectra. In this work, two clones originating from the same immunization gave the same fingerprints. Later, a hybridoma sequencing confirmed the sequence identity of these sister clones. In order to automate the spectral comparison for larger libraries of antibodies, we developed the online software ABID 2.0. This open-source software determines the number of matching peptides in the fingerprint spectra. We propose that publications and other documents critically relying on monoclonal antibodies with unknown amino acid sequences should include at least one antibody fingerprint. By fingerprinting an antibody in question, its identity can be confirmed by comparison with a library spectrum at any time and context.
Instrumental data show that the groundwater and lake levels in Northeast Germany have decreased over the past decades, and this process has accelerated over the past few years. In addition to global warming, the direct influence of humans on the local water balance is suspected to be the cause. Since the instrumental data usually go back only a few decades, little is known about the multidecadal to centennial-scale trend, which also takes long-term climate variation and the long-term influence by humans on the water balance into account. This study aims to quantitatively reconstruct the surface water areas in the Lower Havel Inner Delta and of adjacent Lake Gulpe in Brandenburg. The analysis includes the calculation of surface water areas from historical and modern maps from 1797 to 2020. The major finding is that surface water areas have decreased by approximately 30% since the pre-industrial period, with the decline being continuous. Our data show that the comprehensive measures in Lower Havel hydro-engineering correspond with groundwater lowering that started before recent global warming. Further, large-scale melioration measures with increasing water demands in the upstream wetlands beginning from the 1960s to the 1980s may have amplified the decline in downstream surface water areas.