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We compute seismic velocity profiles by a combined inversion of surface-wave phase-velocity dispersion curves together with the full spectrum of the microtremor horizontal-to-vertical (H/V) spectral ratio at two sediment-covered sites in Germany. The sediment deposits are approximately 100 m thick at the first test site and approximately 400 m thick at the second test site. We have used an extended physical model based on the diffuse wavefield assumption for the interpretation of the observed microtremor H/V spectral ratio. The extension includes the interpretation of the microtremor H/V spectral ratio observed at depth (in boreholes). This full-wavefield approach accounts for the energy contribution from the body and surface waves, and thus it allows for inverting the properties of the shallow subsurface. We have obtained the multimode phase velocity dispersion curves from an independent study, and a description of the extracted branches and their interpretation was developed. The inversion results indicate that the combined approach using seismic ambient noise and actively generated surface-wave data will improve the accuracy of the reconstructed near-surface velocity model, a key step in microzonation, geotechnical engineering, seismic statics corrections, and reservoir imaging.
MALDI-TOF-MS-based identification of monoclonal murine Anti-SARS-CoV-2 antibodies within one hour
(2022)
During the SARS-CoV-2 pandemic, many virus-binding monoclonal antibodies have been developed for clinical and diagnostic purposes. This underlines the importance of antibodies as universal bioanalytical reagents. However, little attention is given to the reproducibility crisis that scientific studies are still facing to date. In a recent study, not even half of all research antibodies mentioned in publications could be identified at all. This should spark more efforts in the search for practical solutions for the traceability of antibodies. For this purpose, we used 35 monoclonal antibodies against SARS-CoV-2 to demonstrate how sequence-independent antibody identification can be achieved by simple means applied to the protein. First, we examined the intact and light chain masses of the antibodies relative to the reference material NIST-mAb 8671. Already half of the antibodies could be identified based solely on these two parameters. In addition, we developed two complementary peptide mass fingerprinting methods with MALDI-TOF-MS that can be performed in 60 min and had a combined sequence coverage of over 80%. One method is based on the partial acidic hydrolysis of the protein by 5 mM of sulfuric acid at 99 degrees C. Furthermore, we established a fast way for a tryptic digest without an alkylation step. We were able to show that the distinction of clones is possible simply by a brief visual comparison of the mass spectra. In this work, two clones originating from the same immunization gave the same fingerprints. Later, a hybridoma sequencing confirmed the sequence identity of these sister clones. In order to automate the spectral comparison for larger libraries of antibodies, we developed the online software ABID 2.0. This open-source software determines the number of matching peptides in the fingerprint spectra. We propose that publications and other documents critically relying on monoclonal antibodies with unknown amino acid sequences should include at least one antibody fingerprint. By fingerprinting an antibody in question, its identity can be confirmed by comparison with a library spectrum at any time and context.