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Pancreatic secretory zymogen-granule membrane glycoprotein 2 (GP2) has been identified to be a major autoantigenic target in Crohn’s disease patients. It was discussed recently that a long and a short isoform of GP2 exists whereas the short isoform is often detected by GP2-specific autoantibodies. In the outcome of inflammatory bowel diseases, these GP2-specific autoantibodies are discussed as new serological markers for diagnosis and therapeutic monitoring. To investigate this further, camelid nanobodies were generated by phage display and selected against the short isoform of GP2 in order to isolate specific tools for the discrimination of both isoforms. Nanobodies are single domain antibodies derived from camelid heavy chain only antibodies and characterized by a high stability and solubility. The selected candidates were expressed, purified and validated regarding their binding properties in different enzyme-linked immunosorbent assays formats, immunofluorescence, immunohistochemistry and surface plasmon resonance spectroscopy. Four different nanobodies could be selected whereof three recognize the short isoform of GP2 very specifically and one nanobody showed a high binding capacity for both isoforms. The KD values measured for all nanobodies were between 1.3 nM and 2.3 pM indicating highly specific binders suitable for the application as diagnostic tool in inflammatory bowel disease.
The geothermal potential in Tarutung is controlled by both the Sumatra Fault system and young arc volcanism. In this study we use the spatial distribution of seismic attenuation, calculated from local earthquake recordings, to image the 3-D seismic attenuation of the area and relate it with the temperature anomalies and the fluid distribution of the subsurface. A temporary seismic network of 42 stations was deployed around Tarutung and Sarulla (south of Tarutung) for a period of 10 months starting in 2011 May. Within this period, the network recorded 2586 local events. A high-quality subset of 229 events recorded by at least 10 stations was used for the attenuation inversion (tomography). Path-average attenuation (t(p)*) was calculated by using a spectral inversion method. The spread function, the contour lines of the model resolution matrix and the recovery test results show that our 3-D attenuation model (Q(p)) has good resolution around the Tarutung Basin and along the Sarulla graben. High attenuation (low Q(p)) related to the geothermal system is found in the northeast of the Tarutung Basin suggesting fluid pathways from below the Sumatra Fault. The upper part of the studied geothermal system in the Tarutung district seems to be mainly controlled by the fault structure rather than by magmatic activities. In the southwest of the Tarutung Basin, the high attenuation zone is associated with the Martimbang volcano. In the Sarulla region, a low-Q(p) anomaly is found along the graben within the vicinity of the Hopong caldera.