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Ferns are among the most popular groups of plants in the tropics and subtropics, and their role as carbon sequestrators has been widely recognized. However, there is little understanding of the silicaceous structures (phytoliths) of ferns, rate of phytolith turnover, the consequences for organic matter sequestered in phytoliths and consequences for other soil properties. In the study reported here, high-resolution X-ray tomographic microscopy and chemical characterization were applied to examine the traits of phytoliths of the fern Dicranopteris linearis (Burm.f.) Underw. (D. linearis), with a focus on their dissolution properties and accumulation in northern Vietnamese soils in relation to soil properties. Tomographic images revealed an inter-embedding structure of silica and organic matter, especially in leaf-derived material. We propose that organic matter and silica can preserve each other against decomposition. In batch experiments, there was a relatively small rate of dissolution of phytoliths with dry ashing and subsequent H2O2 treatment. Silicon (Si) dissolution for D. linearis phytolith samples was much less than that for rice phytoliths. Despite the fact that the aluminum (Al) content was large in D. linearis leaves, batch dissolution data did not confirm a relation between Al and the slow rate of phytolith dissolution. The soil phytolith content varied from 0.9 to 7.5 g kg(-1) in the topsoil across the mountainous areas in northern Vietnam, whereas it tended to be smaller in the subsoil. The data indicate a relation between phytolith and soil organic matter, clay content, oxalate-soluble Al and electrical conductivity, suggesting that these soil properties are among the important factors affecting the size of the soil phytolith Si pool. Highlights
Background
The members of the genus Muntiacus are of particular interest to evolutionary biologists due to their extreme chromosomal rearrangements and the ongoing discussions about the number of living species. Red muntjacs have the largest distribution of all muntjacs and were formerly considered as one species. Karyotype differences led to the provisional split between the Southern Red Muntjac (Muntiacus muntjak) and the Northern Red Muntjac (M. vaginalis), but uncertainties remain as, so far, no phylogenetic study has been conducted. Here, we analysed whole mitochondrial genomes of 59 archival and 16 contemporaneous samples to resolve uncertainties about their taxonomy and used red muntjacs as model for understanding the evolutionary history of other species in Southeast Asia.
Results
We found three distinct matrilineal groups of red muntjacs: Sri Lankan red muntjacs (including the Western Ghats) diverged first from other muntjacs about 1.5 Mya; later northern red muntjacs (including North India and Indochina) and southern red muntjacs (Sundaland) split around 1.12 Mya. The diversification of red muntjacs into these three main lineages was likely promoted by two Pleistocene barriers: one through the Indian subcontinent and one separating the Indochinese and Sundaic red muntjacs. Interestingly, we found a high level of gene flow within the populations of northern and southern red muntjacs, indicating gene flow between populations in Indochina and dispersal of red muntjacs over the exposed Sunda Shelf during the Last Glacial Maximum.
Conclusions
Our results provide new insights into the evolution of species in South and Southeast Asia as we found clear genetic differentiation in a widespread and generalist species, corresponding to two known biogeographical barriers: The Isthmus of Kra and the central Indian dry zone. In addition, our molecular data support either the delineation of three monotypic species or three subspecies, but more importantly these data highlight the conservation importance of the Sri Lankan/South Indian red muntjac.
Background: The members of the genus Muntiacus are of particular interest to evolutionary biologists due to their extreme chromosomal rearrangements and the ongoing discussions about the number of living species. Red muntjacs have the largest distribution of all muntjacs and were formerly considered as one species. Karyotype differences led to the provisional split between the Southern Red Muntjac (Muntiacus muntjak) and the Northern Red Muntjac (M. vaginalis), but uncertainties remain as, so far, no phylogenetic study has been conducted. Here, we analysed whole mitochondrial genomes of 59 archival and 16 contemporaneous samples to resolve uncertainties about their taxonomy and used red muntjacs as model for understanding the evolutionary history of other species in Southeast Asia. Results: We found three distinct matrilineal groups of red muntjacs: Sri Lankan red muntjacs (including the Western Ghats) diverged first from other muntjacs about 1.5 Mya; later northern red muntjacs (including North India and Indochina) and southern red muntjacs (Sundaland) split around 1.12 Mya. The diversification of red muntjacs into these three main lineages was likely promoted by two Pleistocene barriers: one through the Indian subcontinent and one separating the Indochinese and Sundaic red muntjacs. Interestingly, we found a high level of gene flow within the populations of northern and southern red muntjacs, indicating gene flow between populations in Indochina and dispersal of red muntjacs over the exposed Sunda Shelf during the Last Glacial Maximum. Conclusions: Our results provide new insights into the evolution of species in South and Southeast Asia as we found clear genetic differentiation in a widespread and generalist species, corresponding to two known biogeographical barriers: The Isthmus of Kra and the central Indian dry zone. In addition, our molecular data support either the delineation of three monotypic species or three subspecies, but more importantly these data highlight the conservation importance of the Sri Lankan/South Indian red muntjac.