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Foster, William J. ; Garvie, Christopher L. ; Weiss, Anna M. ; Muscente, A. Drew ; Aberhan, Martin ; Counts, John W. ; Martindale, Rowan C.
The hyperthermal events of the Cenozoic, including the Paleocene-Eocene Thermal Maximum, provide an opportunity to investigate the potential effects of climate warming on marine ecosystems. Here, we examine the shallow benthic marine communities preserved in the late Cretaceous to Eocene strata on the Gulf Coastal Plain (United States). In stark contrast to the ecological shifts following the end-Cretaceous mass extinction, our data show that the early Cenozoic hyperthermals did not have a long-term impact on the generic diversity nor composition of the Gulf Coastal Plain molluscan communities. We propose that these communities were resilient to climate change because molluscs are better adapted to high temperatures than other taxa, as demonstrated by their physiology and evolutionary history. In terms of resilience, these communities differ from other shallow-water carbonate ecosystems, such as reef communities, which record significant changes during the early Cenozoic hyperthermals. These data highlight the strikingly different responses of community types, i.e., the almost imperceptible response of molluscs versus the marked turnover of foraminifera and reef faunas. The impact on molluscan communities may have been low because detrimental conditions did not devastate the entire Gulf Coastal Plain, allowing molluscs to rapidly recolonise vacated areas once harsh environmental conditions ameliorated.
Foster, William J. ; Garvie, Christopher L. ; Weiss, Anna M. ; Muscente, A. Drew ; Aberhan, Martin ; Counts, John W. ; Martindale, Rowan C.
The hyperthermal events of the Cenozoic, including the Paleocene-Eocene Thermal Maximum, provide an opportunity to investigate the potential effects of climate warming on marine ecosystems. Here, we examine the shallow benthic marine communities preserved in the late Cretaceous to Eocene strata on the Gulf Coastal Plain (United States). In stark contrast to the ecological shifts following the end-Cretaceous mass extinction, our data show that the early Cenozoic hyperthermals did not have a long-term impact on the generic diversity nor composition of the Gulf Coastal Plain molluscan communities. We propose that these communities were resilient to climate change because molluscs are better adapted to high temperatures than other taxa, as demonstrated by their physiology and evolutionary history. In terms of resilience, these communities differ from other shallow-water carbonate ecosystems, such as reef communities, which record significant changes during the early Cenozoic hyperthermals. These data highlight the strikingly different responses of community types, i.e., the almost imperceptible response of molluscs versus the marked turnover of foraminifera and reef faunas. The impact on molluscan communities may have been low because detrimental conditions did not devastate the entire Gulf Coastal Plain, allowing molluscs to rapidly recolonise vacated areas once harsh environmental conditions ameliorated.
Muiva-Mutisya, Lois M. ; Atilaw, Yoseph ; Heydenreich, Matthias ; Koch, Andreas ; Akala, Hoseah M. ; Cheruiyot, Agnes C. ; Brown, Matthew L. ; Irungu, Beatrice ; Okalebo, Faith A. ; Derese, Solomon ; Mutai, Charles ; Yenesew, Abiy
The CH2Cl2/MeOH (1:1) extract of the aerial parts of Tephrosia subtriflora afforded a new flavanonol, named subtriflavanonol (1), along with the known flavanone spinoflavanone B, and the known flavanonols MS-II (2) and mundulinol. The structures were elucidated by the use of NMR spectroscopy and mass spectrometry. The absolute configuration of the flavanonols was determined based on quantum chemical ECD calculations. In the antiplasmodial assay, compound 2 showed the highest activity against chloroquine-sensitive Plasmodiumfalciparum reference clones (D6 and 3D7), artemisinin-sensitive isolate (F32-TEM) as well as field isolate (KSM 009) with IC50 values 1.4-4.6M without significant cytotoxicity against Vero and HEp2 cell lines (IC50>100M). The new compound (1) showed weak antiplasmodial activity, IC50 12.5-24.2M, but also showed selective anticancer activity against HEp2 cell line (CC50 16.9M). [GRAPHICS] .
Kerubo, Leonidah Omosa ; Midiwo, Jacob Ogweno ; Derese, Solomon ; Langat, Moses K. ; Akala, Hoseah M. ; Waters, Norman C. ; Peter, Martin ; Heydenreich, Matthias
From the surface exudates of Senecio roseiflorus fourteen known methylated flavonoids and one phenol were isolated and characterized. The structures of these compounds were determined on the basis of their spectroscopic analysis. The surface exudate and the flavonoids isolated showed moderate to good antiplasmodial activity with 5,4'-dihydroxy-7-dimethoxyflavanone having the highest activity against chloroquine-sensitive (D6) and resistant (W2) strains of Plasmodium falciparum, with IC50 values of 3.2 +/- 0.8 and 4.4 +/- 0.01 mu g/mL respectively.
Andayi, Andrew W. ; Yenesew, Abiy ; Derese, Solomon ; Midiwo, Jacob O. ; Gitu, Peter M. ; Jondiko, Ogoche J. I. ; Akala, Hoseah M. ; Liyala, Pamela ; Wangui, Julia ; Waters, Norman C. ; Heydenreich, Matthias ; Peter, Martin G.
The acetone extracts of the root bark and stem bark of Erythrina sacleuxii showed antiplasmodial activities against the chloroquine-sensitive (D6) and chloroquine-resistant (W2) strains of Plasmodium falciparum. Chromatographic separation of the acetone extract of the root bark afforded a new isoflavone, 7-hydroxy-4 -methoxy-3'- prenylisoflavone (trivial name 5-deoxy-3' - prenylbiochanin A) along with known isoflavonoids as the antiplasmodial principles. Flavonoids and isoflavonoids isolated from the stem bark of E. sucleuxii were also tested and showed antiplasmodial activities. The structures were determined on the basis of spectroscopic evidence
Peter, Martin G. ; Yenesew, Abiy ; Twinomuhwezi, Hannington ; Kabaru, Jacques M. ; Akala, Hoseah M. ; Kiremire, Bernard T. ; Heydenreich, Matthias ; Eyase, Fredrick ; Waters, Norman C. ; Walsh, Douglas S.
From the dichloromethane-methanol (1:1) extract of the seed pods of Derris trifoliata, a new flavanone derivative (S)-lupinifolin 4´-methyl ether was isolated. In addition, the known flavonoids lupinifolin and rotenone were identified. The structures were determined on the basis of spectroscopic evidence. Lupinfolin showed moderate in vitro antiplasmodial activity against the D6 (chloroquine-sensitive) and W2 (chloroquineresistant) strains of Plasmodium falciparum. The different parts of this plant showed larvicidal activities against Aedes aegypti and rotenoids were identified as the active principles.
Peter, Martin G. ; Muiva, Lois M. ; Yenesew, Abiy ; Derese, Solomon ; Heydenreich, Matthias ; Akala, Hoseah M. ; Eyase, Fredrick ; Waters, Norman C. ; Mutai, Charles ; Keriko, Joseph M. ; Walsh, Douglas S.
From the seedpods of Tephrosia elata, a new β-hydroxydihydrochalcone named (S)-elatadihydrochalcone was isolated. In addition, the known flavonoids obovatachalcone, obovatin, obovatin methyl ether and deguelin were identified. The structures were determined on the basis of spectroscopic evidence. The crude extract and the flavonoids obtained from the seedpods of this plant showed antiplasmodial activities. The literature NMR data on β-hydroxydihydrochalcones is reviewed and the identity of some of the compounds assigned β-hydroxydihydrochalcone skeleton is questioned.
The Atlantic subpolar gyre (SPG) is one of the main drivers of decadal climate variability in the North Atlantic. Here we analyze its dynamics in pre-industrial control simulations of 19 different comprehensive coupled climate models. The analysis is based on a recently proposed description of the SPG dynamics that found the circulation to be potentially bistable due to a positive feedback mechanism including salt transport and enhanced deep convection in the SPG center. We employ a statistical method to identify multiple equilibria in time series that are subject to strong noise and analyze composite fields to assess whether the bistability results from the hypothesized feedback mechanism. Because noise dominates the time series in most models, multiple circulation modes can unambiguously be detected in only six models. Four of these six models confirm that the intensification is caused by the positive feedback mechanism.
Making confident statements about the evolution of an ice-sheet shelf system with a numerical model requires the capability to reproduce the migration of the grounding line. Here we show that the shallow-ice approximation/shallow-shelf approximation hybrid-type Parallel Ice Sheet Model (PISM), with its recent improvements, is capable of modeling the grounding line motion in a perturbed ice-sheet shelf system. The model is set up according to the three-dimensional Marine Ice-Sheet Model Intercomparison Project (MISMIP3d), and simulations are carried out across a broad range of spatial resolutions. Using (1) a linear interpolation of the grounding line with locally interpolated basal friction and (2) an improved driving-stress computation across the grounding line, the reversibility of the grounding line (i.e. its retreat after an advance forced by a local perturbation of basal resistance) is captured by the model even at medium and low resolutions (Delta x > 10 km). The transient model response is qualitatively similar to that of higher-order models but reveals a higher initial sensitivity to perturbations on very short timescales. Our findings support the application of PISM to the Antarctic ice sheet from regional up to continental scales and on relatively low spatial resolutions.
Ein Dach über Europa
(2022)
Wo ist Deutschlands Raketenabwehr? Diese Frage rückte nach der völkerrechtswidrigen Annexion der Krim durch Russland 2014 in den Fokus der Presseberichterstattung. Für die Abwehr von ballistischen Raketen ist die Flugabwehrraketentruppe der Luftwaffe zuständig. Im Ost-West-Konflikt schützten rund 18.600 deutsche Soldaten im Rahmen der Integrierten NATO-Luftverteidigung die westliche Allianz vor Luftangriffen durch den Warschauer Pakt. Nach der Wiedervereinigung befand sich der Luftverteidigungsgürtel des Bündnisses nicht nur in einer geografisch wirkungslosen Position, sondern ihm fehlte auch die Daseinsberechtigung. Mit seiner Auflösung ging ein erheblicher Abbau von Personal und Material der Flugabwehrraketenverbände einher. Nach der Neuausrichtung der Bundeswehr 2012 blieb diesem Dienstbereich der Luftwaffe nur noch ein Geschwader mit rund 2.300 Dienstposten. Der alte Feind war weg – und Deutschland nach 1989/90 umgeben von Freunden und Verbündeten. Warum also sollte die Regierung in eine Fähigkeit investieren, die Deutschland für sich selbst nicht brauchte?
E-Learning Symposium 2018
(2018)
Keil, Reinhard ; Konert, Johannes ; Damnik, Gregor ; Gierl, Mark J. ; Proske, Antje ; Körndle, Hermann ; Narciss, Susanne ; Wahl, Marina ; Hölscher, Michael ; Mariani, Ennio ; Jaisli, Isabel ; Tscherejkina, Anna ; Morgiel, Anna ; Moebert, Tobias ; Herbstreit, Stephanie ; Mäker, Daniela ; Szalai, Cynthia ; Braun, Iris ; Kapp, Felix ; Hara, Tenshi C. ; Kubica, Tommy ; Stumpf, Sarah
In den vergangenen Jahren sind viele E-Learning-Innovationen entstanden. Einige davon wurden auf den vergangenen E-Learning Symposien der Universität Potsdam präsentiert: Das erste E-Learning Symposium im Jahr 2012 konzentrierte sich auf unterschiedliche Möglichkeiten der Studierendenaktivierung und Lehrgestaltung. Das Symposium 2014 rückte vor allem die Studierenden ins Zentrum der Aufmerksamkeit. Im Jahr 2016 kam es durch das Zusammengehen des Symposiums mit der DeLFI-Tagung zu einer Fokussierung auf technische Innovationen. Doch was ist aus den Leuchttürmen von gestern geworden, und brauchen wir überhaupt noch neue Leuchttürme? Das Symposium setzt sich in diesem Jahr unter dem Motto „Innovation und Nachhaltigkeit – (k)ein Gegensatz?“ mit mediengestützten Lehr- und Lernprozessen im universitären Kontext auseinander und reflektiert aktuelle technische sowie didaktische Entwicklungen mit Blick auf deren mittel- bis langfristigen Einsatz in der Praxis.
Dieser Tagungsband zum E-Learning Symposium 2018 an der Universität Potsdam beinhaltet eine Mischung von Forschungs- und Praxisbeiträgen aus verschiedenen Fachdisziplinen und eröffnet vielschichtige Perspektiven auf das Thema E-Learning. Dabei werden die Vielfalt der didaktischen Einsatzszenarien als auch die Potentiale von Werk-zeugen und Methoden der Informatik in ihrem Zusammenspiel beleuchtet.
In seiner Keynote widmet sich Reinhard Keil dem Motto des Symposiums und geht der Nachhaltigkeit bei E-Learning-Projekten auf den Grund. Dabei analysiert und beleuchtet er anhand seiner über 15-jährigen Forschungspraxis die wichtigsten Wirkfaktoren und formuliert Empfehlungen zur Konzeption von E-Learning-Projekten. Im Gegensatz zu rein auf Kostenersparnis ausgerichteten (hochschul-)politischen Forderungen proklamiert er den Ansatz der hypothesengeleiteten Technikgestaltung, in der Nachhaltigkeit als Leitfrage oder Forschungsstrategie verstanden werden kann. In eine ähnliche Richtung geht der Beitrag von Iris Braun et al., die über Erfolgsfaktoren beim Einsatz von Audience Response Systemen in der universitären Lehre berichten.
Ein weiteres aktuelles Thema, sowohl für die Bildungstechnologie als auch in den Bildungswissenschaften allgemein, ist die Kompetenzorientierung und –modellierung. Hier geht es darum (Problemlöse-)Fähigkeiten gezielt zu beschreiben und in den Mittelpunkt der Lehre zu stellen. Johannes Konert stellt in einem eingeladenen Vortrag zwei Projekte vor, die den Prozess beginnend bei der Definition von Kompetenzen, deren Modellierung in einem semantischen maschinenlesbaren Format bis hin zur Erarbeitung von Methoden zur Kompetenzmessung und der elektronischen Zertifizierung aufzeigen. Dabei geht er auf technische Möglichkeiten, aber auch Grenzen ein. Auf einer spezifischeren Ebene beschäftigt sich Sarah Stumpf mit digitalen bzw. mediendidaktischen Kompetenzen im Lehramtsstudium und stellt ein Framework für die Förderung ebensolcher Kompetenzen bei angehenden Lehrkräften vor.
Der Einsatz von E-Learning birgt noch einige Herausforderungen. Dabei geht es oft um die Verbindung von Didaktik und Technik, den Erhalt von Aufmerksamkeit oder den Aufwand für das Erstellen von interaktiven Lehr- und Lerninhalten. Drei Beiträge in diesem Tagungsband beschäftigen sich mit dieser Thematik in unterschiedlichen Kontexten und zeigen Best-Practices und Lösungsansätze auf: Der Beitrag von Martina Wahl und Michael Hölscher behandelt den besonderen Kontext von Blended Learning-Szenarien in berufsbegleitenden Studiengängen. Um die Veröffentlichung eines global frei verfügbaren Onlinekurses abseits der großen MOOC Plattformen und den didaktischen Herausforderungen auch hinsichtlich der Motivation geht es im Beitrag von Ennio Marani und Isabel Jaisli. Schließlich schlagen Gregor Damnik et al. die automatische Erzeugung von Aufgaben zur Erhöhung von Interaktivität und Adaptivität in digitalen Lernressourcen vor, um den teilweise erheblichen Erstellungsaufwand zu reduzieren.
Zum Thema E-Learning zählen auch immer mobile Apps bzw. Spiele. Gleich zwei Beiträge beschäftigen sich mit dem Einsatz von E-Learning-Tools im Gesundheitskontext: Anna Tscherejkina und Anna Morgiel stellen in ihrem Beitrag Minispiele zum Training von sozio-emotionalen Kompetenzen für Menschen mit Autismus vor, und Stephanie Herbstreit et al. berichten vom Einsatz einer mobilen Lern-App zur Verbesserung von klinisch-praktischem Unterricht.
Troll, K. ; Kulkarni, Amit ; Wang, W. ; Darko, C. ; Koumba, A. M. Bivigou ; Laschewsky, André ; Müller-Buschbaum, Peter ; Papadakis, Christine M.
Cordeiro, Andre M. ; Andrade, Luis ; Monteiro, Catarina C. ; Leitao, Guilherme ; Wigge, Philip Anthony ; Saibo, Nelson J. M.
Review exploring the regulation of PHYTOCHROME-INTERACTING FACTORS by light, their role in abiotic stress tolerance and plant architecture, and their influence on crop productivity.
Light is a key determinant for plant growth, development, and ultimately yield. Phytochromes, red/far-red photoreceptors, play an important role in plant architecture, stress tolerance, and productivity. In the model plant Arabidopsis, it has been shown that PHYTOCHROME-INTERACTING FACTORS (PIFs; bHLH transcription factors) act as central hubs in the integration of external stimuli to regulate plant development. Recent studies have unveiled the importance of PIFs in crops. They are involved in the modulation of plant architecture and productivity through the regulation of cell division and elongation in response to different environmental cues. These studies show that different PIFs have overlapping but also distinct functions in the regulation of plant growth. Therefore, understanding the molecular mechanisms by which PIFs regulate plant development is crucial to improve crop productivity under both optimal and adverse environmental conditions. In this review, we discuss current knowledge of PIFs acting as integrators of light and other signals in different crops, with particular focus on the role of PIFs in responding to different environmental conditions and how this can be used to improve crop productivity.
Sommer, C. ; Garbusow, Maria ; Juenger, E. ; Pooseh, S. ; Bernhardt, Nadine ; Birkenstock, J. ; Schad, Daniel ; Jabs, B. ; Gloeckler, T. ; Huys, Quentin J. M. ; Heinz, A. ; Smolka, Michael N. ; Zimmermann, Ulrich S.
Alcohol-related cues acquire incentive salience through Pavlovian conditioning and then can markedly affect instrumental behavior of alcohol-dependent patients to promote relapse. However, it is unclear whether similar effects occur with alcohol-unrelated cues. We tested 116 early-abstinent alcohol-dependent patients and 91 healthy controls who completed a delay discounting task to assess choice impulsivity, and a Pavlovian-to-instrumental transfer (PIT) paradigm employing both alcohol-unrelated and alcohol-related stimuli. To modify instrumental choice behavior, we tiled the background of the computer screen either with conditioned stimuli (CS) previously generated by pairing abstract pictures with pictures indicating monetary gains or losses, or with pictures displaying alcohol or water beverages. CS paired to money gains and losses affected instrumental choices differently. This PIT effect was significantly more pronounced in patients compared to controls, and the group difference was mainly driven by highly impulsive patients. The PIT effect was particularly strong in trials in which the instrumental stimulus required inhibition of instrumental response behavior and the background CS was associated to monetary gains. Under that condition, patients performed inappropriate approach behavior, contrary to their previously formed behavioral intention. Surprisingly, the effect of alcohol and water pictures as background stimuli resembled that of aversive and appetitive CS, respectively. These findings suggest that positively valenced background CS can provoke dysfunctional instrumental approach behavior in impulsive alcohol-dependent patients. Consequently, in real life they might be easily seduced by environmental cues to engage in actions thwarting their long-term goals. Such behaviors may include, but are not limited to, approaching alcohol.
Tadjoung Waffo, Armel Franklin ; Yesildag, Cigdem ; Caserta, Giorgio ; Katz, Sagie ; Zebger, Ingo ; Lensen, Marga C. ; Wollenberger, Ulla ; Scheller, Frieder W. ; Altintas, Zeynep
This study aims to develop a rapid, sensitive and cost-effective biomimetic electrochemical sensor for artemisinin determination in plant extracts and for pharmacokinetic studies. A novel molecularly imprinted polymer (MIP)based electrochemical sensor was developed by electropolymerization of o-phenylenediamine (o-PD) in the presence of artemisinin on gold wire surface for sensitive detection of artemisinin. The experimental parameters, including selection of functional monomer, polymerization conditions, template extraction after polymerization, influence of pH and buffer were all optimized. Every step of imprinted film synthesis were evaluated by employing voltammetry techniques, surface-enhanced infrared absorption spectroscopy (SEIRAS) and atomic force microscopy (AFM). The specificity was further evaluated by investigating non-specific artemisinin binding on non-imprinted polymer (NIP) surfaces and an imprinting factor of 6.8 was achieved. The artemisinin imprinted polymers using o-PD as functional monomer have provided highly stable and effective binding cavities for artemisinin. Cross-reactivity studies with drug molecules showed that the MIPs are highly specific for artemisinin. The influence of matrix effect was further investigated both in artificial plant matrix and diluted human serum. The results revealed a high affinity of artemisinin-MIP with dissociation constant of 7.3 x 10(-9) M and with a detection limit of 0.01 mu M and 0.02 mu M in buffer and plant matrix, respectively.
McNulty, Margaret A. ; Goupil, Brad A. ; Albarado, Diana C. ; Castaño-Martinez, Teresa ; Ambrosi, Thomas H. ; Puh, Spela ; Schulz, Tim Julius ; Schürmann, Annette ; Morrison, Christopher D. ; Laeger, Thomas
Background: Dietary protein restriction is emerging as an alternative approach to treat obesity and glucose intolerance because it markedly increases plasma fibroblast growth factor 21 (FGF21) concentrations. Similarly, dietary restriction of methionine is known to mimic metabolic effects of energy and protein restriction with FGF21 as a required mechanism. However, dietary protein has been shown to be required for normal bone growth, though there is conflicting evidence as to the influence of dietary protein restriction on bone remodeling. The purpose of the current study was to evaluate the effect of dietary protein and methionine restriction on bone in lean and obese mice, and clarify whether FGF21 and general control nonderepressible 2 (GCN2) kinase, that are part of a novel endocrine pathway implicated in the detection of protein restriction, influence the effect of dietary protein restriction on bone.
Methods: Adult wild-type (WT) or Fgf21 KO mice were fed a normal protein (18 kcal%; CON) or low protein (4 kcal%; LP) diet for 2 or 27 weeks. In addition, adult WT or Gcn2 KO mice were fed a CON or LP diet for 27 weeks. Young New Zealand obese (NZO) mice were placed on high-fat diets that provided protein at control (16 kcal%; CON), low levels (4 kcal%) in a high-carbohydrate (LP/HC) or high-fat (LP/HF) regimen, or on high-fat diets (protein, 16 kcal%) that provided methionine at control (0.86%; CON-MR) or low levels (0.17%; MR) for up to 9 weeks. Long bones from the hind limbs of these mice were collected and evaluated with micro-computed tomography (mu CT) for changes in trabecular and cortical architecture and mass.
Results: In WT mice the 27-week LP diet significantly reduced cortical bone, and this effect was enhanced by deletion of Fgf21 but not Gcn2. This decrease in bone did not appear after 2 weeks on the LP diet. In addition, Fgf21 KO mice had significantly less bone than their WT counterparts. In obese NZO mice dietary protein and methionine restriction altered bone architecture. The changes were mediated by FGF21 due to methionine restriction in the presence of cystine, which did not increase plasma FGF21 levels and did not affect bone architecture.
Conclusions: This study provides direct evidence of a reduction in bone following long-term dietary protein restriction in a mouse model, effects that appear to be mediated by FGF21.
Estimating global mean sea-level rise and its uncertainties by 2100 and 2300 from an expert survey
(2020)
Horton, Benjamin P. ; Khan, Nicole S. ; Cahill, Niamh ; Lee, Janice S. H. ; Shaw, Timothy A. ; Garner, Andra J. ; Kemp, Andrew C. ; Engelhart, Simon E. ; Rahmstorf, Stefan
Sea-level rise projections and knowledge of their uncertainties are vital to make informed mitigation and adaptation decisions. To elicit projections from members of the scientific community regarding future global mean sea-level (GMSL) rise, we repeated a survey originally conducted five years ago. Under Representative Concentration Pathway (RCP) 2.6, 106 experts projected a likely (central 66% probability) GMSL rise of 0.30-0.65 m by 2100, and 0.54-2.15 m by 2300, relative to 1986-2005. Under RCP 8.5, the same experts projected a likely GMSL rise of 0.63-1.32 m by 2100, and 1.67-5.61 m by 2300. Expert projections for 2100 are similar to those from the original survey, although the projection for 2300 has extended tails and is higher than the original survey. Experts give a likelihood of 42% (original survey) and 45% (current survey) that under the high-emissions scenario GMSL rise will exceed the upper bound (0.98 m) of the likely range estimated by the Fifth Assessment Report of the Intergovernmental Panel on Climate Change, which is considered to have an exceedance likelihood of 17%. Responses to open-ended questions suggest that the increases in upper-end estimates and uncertainties arose from recent influential studies about the impact of marine ice cliff instability on the meltwater contribution to GMSL rise from the Antarctic Ice Sheet.
Hoke, Gregory D. ; Garzione, Carmala N. ; Araneo, Diego C. ; Latorre, Claudio ; Strecker, Manfred ; Williams, Kendra J.
Stable isotope altimetry is a useful tool for estimating paleoelevation in sedimentary records. Yet questions remain regarding how source moisture, climate, and local topography can influence these estimates. Here we present stable isotope altimetry results on late Quaternary pedogenic carbonates of known elevation on both flanks of the Andean orogen at 33 degrees S. We measured delta O-18 values of pedogenic carbonates and river water samples from small drainages at regular elevation increments within the Rio Aconcagua (Chile) and Rio Mendoza (Argentina) catchments. The delta O-18 values of river waters correlate well with elevation and show similar isotopic gradients between the Chilean (-3.7 parts per thousand/km) and Argentine (-4.8 parts per thousand/km) sides of the range. Uncertainties associated with scatter in the river water data and assumptions about the temperature of carbonate formation indicate that elevation estimates have 1 sigma errors of 350-450 m. We estimate the isotopic composition of soil water from pedogenic carbonates on both sides of the range by assuming mean annual temperatures based the modern temperature lapse rate from meteorological station data. Combined, our data show that stable isotope altimetry produces reasonable estimates of modern elevation, with the majority of our samples (60%) within the 1 sigma uncertainties and 77% within 2 sigma.
McNulty, Margaret A. ; Goupil, Brad A. ; Albarado, Diana C. ; Castaño-Martinez, Teresa ; Ambrosi, Thomas H. ; Puh, Spela ; Schulz, Tim Julius ; Schürmann, Annette ; Morrison, Christopher D. ; Laeger, Thomas
Background: Dietary protein restriction is emerging as an alternative approach to treat obesity and glucose intolerance because it markedly increases plasma fibroblast growth factor 21 (FGF21) concentrations. Similarly, dietary restriction of methionine is known to mimic metabolic effects of energy and protein restriction with FGF21 as a required mechanism. However, dietary protein has been shown to be required for normal bone growth, though there is conflicting evidence as to the influence of dietary protein restriction on bone remodeling. The purpose of the current study was to evaluate the effect of dietary protein and methionine restriction on bone in lean and obese mice, and clarify whether FGF21 and general control nonderepressible 2 (GCN2) kinase, that are part of a novel endocrine pathway implicated in the detection of protein restriction, influence the effect of dietary protein restriction on bone.
Methods: Adult wild-type (WT) or Fgf21 KO mice were fed a normal protein (18 kcal%; CON) or low protein (4 kcal%; LP) diet for 2 or 27 weeks. In addition, adult WT or Gcn2 KO mice were fed a CON or LP diet for 27 weeks. Young New Zealand obese (NZO) mice were placed on high-fat diets that provided protein at control (16 kcal%; CON), low levels (4 kcal%) in a high-carbohydrate (LP/HC) or high-fat (LP/HF) regimen, or on high-fat diets (protein, 16 kcal%) that provided methionine at control (0.86%; CON-MR) or low levels (0.17%; MR) for up to 9 weeks. Long bones from the hind limbs of these mice were collected and evaluated with micro-computed tomography (mu CT) for changes in trabecular and cortical architecture and mass.
Results: In WT mice the 27-week LP diet significantly reduced cortical bone, and this effect was enhanced by deletion of Fgf21 but not Gcn2. This decrease in bone did not appear after 2 weeks on the LP diet. In addition, Fgf21 KO mice had significantly less bone than their WT counterparts. In obese NZO mice dietary protein and methionine restriction altered bone architecture. The changes were mediated by FGF21 due to methionine restriction in the presence of cystine, which did not increase plasma FGF21 levels and did not affect bone architecture.
Conclusions: This study provides direct evidence of a reduction in bone following long-term dietary protein restriction in a mouse model, effects that appear to be mediated by FGF21.