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I. Staat und Kommunen: Ein Weg oder viele in Europa? II. Ausgangspunkte: Spielarten kommunaler Selbstverwaltung in Europa III. Reform der intergouvernementalen Beziehungen zwischen Dezentralisierung und Zentralisierung, Verstaatlichung und lokaler Autonomie IV. Entwicklungslinien: Reformen der intergouvernementalen Beziehungen in Deutschland, Frankreich und Großbritannien V. Europäische Kommunen zwischen Konvergenz und nationaler Eigenlogik. Und schließlich doch: Welche Rolle spielt die EU?
I. Reformbedarf II. Konsequenzen III. Funktionalreformen IV. Bausteine einer umfassenden Verwaltungsmodernisierung V. Das Leitbild „Gemeinde der Zukunft“ VI. Freiwilligkeitsphase VII. Zukunftsvertrag zwischen Land und Kommunen VIII. Zur FAG-Änderung 2012 IX. Haushaltskonsolidierung und Kommunaler Haushaltskonsolidierungsfonds X. Kofinanzierungsfonds XI. Fazit
GEOPHILUS ELECTRICUS (nickname GEOPHILUS) is a novel system for mapping the complex electrical bulk resistivity of soils. Rolling electrodes simultaneously measure amplitude and phase data at frequencies ranging from 1 mHz to 1 kHz. The sensor's design and technical specifications allow for measuring these parameters at five depths of up to ca. 1.5 m. Data inversion techniques can be employed to determine resistivity models instead of apparent values and to image soil layers and their geometry with depth. When used in combination with a global positioning system (GPS) and a suitable cross-country vehicle, it is possible to map about 100 ha/day (assuming 1 data point is recorded per second and the line spacing is 18 m). The applicability of the GEOPHILUS system has been demonstrated on several sites, where soils show variations in texture, stratification, and thus electrical characteristics. The data quality has been studied by comparison with 'static' electrodes, by repeated measurements, and by comparison with other mobile conductivity mapping devices (VERIS3100 and EM38). The high quality of the conductivity data produced by the GEOPHILUS system is evident and demonstrated by the overall consistency of the individual maps, and in the clear stratification also confirmed by independent data.
The GEOPHILUS system measures complex values of electrical resistivity in terms of amplitude and phase. Whereas electrical conductivity data (amplitude) are well established in soil science, the interpretation of phase data is a topic of current research. Whether phase data are able to provide additional information depends on the site-specific settings. Here, we present examples, where phase data provide complementary information on man-made structures such as metal pipes and soil compaction.
Background: DNA fragments carrying internal recognition sites for the restriction endonucleases intended for cloning into a target plasmid pose a challenge for conventional cloning.
Results: A method for directional insertion of DNA fragments into plasmid vectors has been developed. The target sequence is amplified from a template DNA sample by PCR using two oligonucleotides each containing a single deoxyinosine base at the third position from the 5' end. Treatment of such PCR products with endonuclease V generates 3' protruding ends suitable for ligation with vector fragments created by conventional restriction endonuclease reactions.
Conclusions: The developed approach generates terminal cohesive ends without the use of Type II restriction endonucleases, and is thus independent from the DNA sequence. Due to PCR amplification, minimal amounts of template DNA are required. Using the robust Taq enzyme or a proofreading Pfu DNA polymerase mutant, the method is applicable to a broad range of insert sequences. Appropriate primer design enables direct incorporation of terminal DNA sequence modifications such as tag addition, insertions, deletions and mutations into the cloning strategy. Further, the restriction sites of the target plasmid can be either retained or removed.
Background: The linear noise approximation (LNA) is commonly used to predict how noise is regulated and exploited at the cellular level. These predictions are exact for reaction networks composed exclusively of first order reactions or for networks involving bimolecular reactions and large numbers of molecules. It is however well known that gene regulation involves bimolecular interactions with molecule numbers as small as a single copy of a particular gene. It is therefore questionable how reliable are the LNA predictions for these systems.
Results: We implement in the software package intrinsic Noise Analyzer (iNA), a system size expansion based method which calculates the mean concentrations and the variances of the fluctuations to an order of accuracy higher than the LNA. We then use iNA to explore the parametric dependence of the Fano factors and of the coefficients of variation of the mRNA and protein fluctuations in models of genetic networks involving nonlinear protein degradation, post-transcriptional, post-translational and negative feedback regulation. We find that the LNA can significantly underestimate the amplitude and period of noise-induced oscillations in genetic oscillators. We also identify cases where the LNA predicts that noise levels can be optimized by tuning a bimolecular rate constant whereas our method shows that no such regulation is possible. All our results are confirmed by stochastic simulations.
Conclusion: The software iNA allows the investigation of parameter regimes where the LNA fares well and where it does not. We have shown that the parametric dependence of the coefficients of variation and Fano factors for common gene regulatory networks is better described by including terms of higher order than LNA in the system size expansion. This analysis is considerably faster than stochastic simulations due to the extensive ensemble averaging needed to obtain statistically meaningful results. Hence iNA is well suited for performing computationally efficient and quantitative studies of intrinsic noise in gene regulatory networks.
Small and medium-sized enterprises (SME) contribute to innovation and economic growth, despite their resource shortages and lack of professional intellectual property (IP) management practices. Drawing on social practice theory and combining insights from recent scholarship on IP strategies and its management, this paper examines the cases of three pharmaceutical SME providing insights into how they appropriate returns on research and development (R&D) investments. It discusses their IP strategies and management practices, examining how the IP management practices are embedded in the firm's organisational structure. Moreover, this paper develops recommendations for SME regarding the professionalisation of their IP management practices.
How preview space/time translates into preview cost/benefit for fixation durations during reading
(2013)
Eye-movement control during reading depends on foveal and parafoveal information. If the parafoveal preview of the next word is suppressed, reading is less efficient. A linear mixed model (LMM) reanalysis of McDonald (2006) confirmed his observation that preview benefit may be limited to parafoveal words that have been selected as the saccade target. Going beyond the original analyses, in the same LMM, we examined how the preview effect (i.e., the difference in single-fixation duration, SFD, between random-letter and identical preview) depends on the gaze duration on the pretarget word and on the amplitude of the saccade moving the eye onto the target word. There were two key results: (a) The shorter the saccade amplitude (i.e., the larger preview space), the shorter a subsequent SFD with an identical preview; this association was not observed with a random-letter preview. (b) However, the longer the gaze duration on the pretarget word, the longer the subsequent SFD on the target, with the difference between random-letter string and identical previews increasing with preview time. A third patternincreasing cost of a random-letter string in the parafovea associated with shorter saccade amplitudeswas observed for target gaze durations. Thus, LMMs revealed that preview effects, which are typically summarized under preview benefit, are a complex mixture of preview cost and preview benefit and vary with preview space and preview time. The consequence for reading is that parafoveal preview may not only facilitate, but also interfere with lexical access.
In this paper, we determine necessary and sufficient conditions for Bruck-Reilly and generalized Bruck-Reilly ∗-extensions of arbitrary monoids to be regular, coregular and strongly π-inverse. These semigroup classes have applications in various field of mathematics, such as matrix theory, discrete mathematics and p-adic analysis (especially in operator theory). In addition, while regularity and coregularity have so many applications in the meaning of boundaries (again in operator theory), inverse monoids and Bruck-Reilly extensions contain a mixture fixed-point results of algebra, topology and geometry within the purposes of this journal.