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Cavitation at the solid surface normally begins with nucleation, in which defects or assembled molecules located at a liquid-solid interface act as nucleation centers and are actively involved in the evolution of cavitation bubbles. Here, we propose a simple approach to evaluate the behavior of cavitation bubbles formed under high intensity ultrasound (20 kHz, 51.3 W cm (2)) at solid surfaces, based on sonication of patterned substrates with a small roughness (less than 3 nm) and controllable surface energy. A mixture of octadecylphosphonic acid (ODTA) and octadecanethiol (ODT) was stamped on the Si wafer coated with different thicknesses of an aluminium layer (20-500 nm). We investigated the growth mechanism of cavitation bubble nuclei and the evolution of individual pits (defects) formed under sonication on the modified surface. A new activation behavior as a function of Al thickness, sonication time, ultrasonic power and temperature is reported. In this process cooperativity is introduced, as initially formed pits further reduce the energy to form bubbles. Furthermore, cavitation on the patterns is a controllable process, where up to 40-50 min of sonication time only the hydrophobic areas are active nucleation sites. This study provides a convincing proof of our theoretical approach on nucleation.
Identification of a novel heteroglycan-interacting protein, HIP 1.3, from Arabidopsis thaliana
(2011)
Plastidial degradation of transitory starch yields mainly maltose and glucose. Following the export into the cytosol, maltose acts as donor for a glucosyl transfer to cytosolic heteroglycans as mediated by a cytosolic transglucosidase (DPE2; EC 2.4.1.25) and the second glucosyl residue is liberated as glucose. The cytosolic phosphorylase (Pho2/PHS2; EC 2.4.1.1) also interacts with heteroglycans using the same intramolecular sites as DPE2. Thus, the two glucosyl transferases interconnect the cytosolic pools of glucose and glucose 1-phosphate. Due to the complex monosaccharide pattern, other heteroglycan-interacting proteins (Hips) are expected to exist.
Identification of those proteins was approached by using two types of affinity chromatography. Heteroglycans from leaves of Arabidopsis thaliana (Col-0) covalently bound to Sepharose served as ligands that were reacted with a complex mixture of buffer-soluble proteins from Arabidopsis leaves. Binding proteins were eluted by sodium chloride. For identification, SDS-PAGE, tryptic digestion and MALDI-TOF analyses were applied. A strongly interacting polypeptide (approximately 40 kDa; designated as HIP1.3) was observed as product of locus At1g09340. Arabidopsis mutants deficient in HIP1.3 were reduced in growth and contained heteroglycans displaying an altered monosaccharide pattern. Wild type plants express HIP1.3 most strongly in leaves. As revealed by immuno fluorescence, HIP1.3 is located in the cytosol of mesophyll cells but mostly associated with the cytosolic surface of the chloroplast envelope membranes. In an HIP1.3-deficient mutant the immunosignal was undetectable. Metabolic profiles from leaves of this mutant and wild type plants as well were determined by GC-MS. As compared to the wild type control, more than ten metabolites, such as ascorbic acid, fructose, fructose bisphosphate, glucose, glycine, were elevated in darkness but decreased in the light. Although the biochemical function of HIP1.3 has not yet been elucidated, it is likely to possess an important function in the central carbon metabolism of higher plants.
Maturation of fleshy fruits such as tomato (Solanum lycopersicum) is subject to tight genetic control. Here we describe the development of a quantitative real-time PCR platform that allows accurate quantification of the expression level of approximately 1000 tomato transcription factors. In addition to utilizing this novel approach, we performed cDNA microarray analysis and metabolite profiling of primary and secondary metabolites using GC-MS and LC-MS, respectively. We applied these platforms to pericarp material harvested throughout fruit development, studying both wild-type Solanum lycopersicum cv. Ailsa Craig and the hp1 mutant. This mutant is functionally deficient in the tomato homologue of the negative regulator of the light signal transduction gene DDB1 from Arabidopsis, and is furthermore characterized by dramatically increased pigment and phenolic contents. We choose this particular mutant as it had previously been shown to have dramatic alterations in the content of several important fruit metabolites but relatively little impact on other ripening phenotypes. The combined dataset was mined in order to identify metabolites that were under the control of these transcription factors, and, where possible, the respective transcriptional regulation underlying this control. The results are discussed in terms of both programmed fruit ripening and development and the transcriptional and metabolic shifts that occur in parallel during these processes.
Linear mixed models (LMMs) provide a still underused methodological perspective on combining experimental and individual-differences research. Here we illustrate this approach with two-rectangle cueing in visual attention (Egly et al., 1994). We replicated previous experimental cue-validity effects relating to a spatial shift of attention within an object (spatial effect), to attention switch between objects (object effect), and to the attraction of attention toward the display centroid (attraction effect), also taking into account the design-inherent imbalance of valid and other trials. We simultaneously estimated variance/covariance components of subject-related random effects for these spatial, object, and attraction effects in addition to their mean reaction times (RTs). The spatial effect showed a strong positive correlation with mean RT and a strong negative correlation with the attraction effect. The analysis of individual differences suggests that slow subjects engage attention more strongly at the cued location than fast subjects. We compare this joint LMM analysis of experimental effects and associated subject-related variances and correlations with two frequently used alternative statistical procedures.
There has been a substantial increase in the percentage for publications with co-authors located in departments from different countries in 12 major journals of psychology. The results are evidence for a remarkable internationalization of psychological research, starting in the mid 1970s and increasing in rate at the beginning of the 1990s. This growth occurs against a constant number of articles with authors from the same country; it is not due to a concomitant increase in the number of co-authors per article. Thus, international collaboration in psychology is obviously on the rise.
Mit der lexikalischen Datenbank dlexDB stellen wir der psychologischen und linguistischen Forschung im World Wide Web online statistische Kennwerte für eine Vielzahl von verarbeitungsrelevanten Merkmalen von Wörtern zur Verfügung. Diese Kennwerte umfassen die durch CELEX (Baayen, Piepenbrock und Gulikers, 1995) bekannten Variablen der Häufigkeiten von Wortformen und Lemmata in Texten geschriebener Sprache. Darüber hinaus berechnen wir eine Reihe neuer Kennwerte wie die Häufigkeiten von Silben, Morphemen, Zeichenfolgen und Mehrwortverbindungen sowie Wortähnlichkeitsmaße. Die Datengrundlage bildet das Kernkorpus des Digitalen Wörterbuchs der deutschen Sprache (DWDS) mit über 100 Millionen laufenden Wörtern. Wir illustrieren die Validität dieser Kennwerte mit neuen Ergebnissen zu ihrem Einfluss auf Fixationsdauern beim Lesen von Sätzen.
Following up on research suggesting an age-related reduction in the rightward extent of the perceptual span during reading (Rayner, Castelhano, & Yang, 2009), we compared old and young adults in an N + 2-boundary paradigm in which a nonword preview of word N + 2 or word N + 2 itself is replaced by the target word once the eyes cross an invisible boundary located after word N. The intermediate word N + 1 was always three letters long. Gaze durations on word N + 2 were significantly shorter for identical than nonword N + 2 preview both for young and for old adults, with no significant difference in this preview benefit. Young adults, however, did modulate their gaze duration on word N more strongly than old adults in response to the difficulty of the parafoveal word N + 1. Taken together, the results suggest a dissociation of preview benefit and parafoveal-on-foveal effect. Results are discussed in terms of age-related decline in resilience towards distributed processing while simultaneously preserving the ability to integrate parafoveal information into foveal processing. As such, the present results relate to proposals of regulatory compensation strategies older adults use to secure an overall reading speed very similar to that of young adults. (PsycINFO Database Record (c) 2011 APA, all rights reserved)
Eye fixation durations during normal reading correlate with processing difficulty, but the specific cognitive mechanisms reflected in these measures are not well understood. This study finds support in German readers' eye fixations for two distinct difficulty metrics: surprisal, which reflects the change in probabilities across syntactic analyses as new words are integrated; and retrieval, which quantifies comprehension difficulty in terms of working memory constraints. We examine the predictions of both metrics using a family of dependency parsers indexed by an upper limit on the number of candidate syntactic analyses they retain at successive words. Surprisal models all fixation measures and regression probability. By contrast, retrieval does not model any measure in serial processing. As more candidate analyses are considered in parallel at each word, retrieval can account for the same measures as surprisal. This pattern suggests an important role for ranked parallelism in theories of sentence comprehension.
We examine how the size of saccadic under-/overshoot and target eccentricity influence the latency, amplitude and orientation of secondary (micro-)saccades. In our experiment, a target appeared at an eccentricity of either 6 degrees or 14 degrees of visual angle. Subjects were instructed to direct their gaze as quickly as possible to the target and hold fixation at the new location until the end of the trial. Typically, increasing saccadic error is associated with faster and larger secondary saccades. We show that secondary saccades at distant in contrast to close targets have in a specific error range a shorter latency, larger amplitude, and follow more often the direction of the primary saccade. Finally, we demonstrate that an undershooting primary saccade is followed almost exclusively by secondary saccades into the same direction while overshooting primary saccades are followed by secondary saccades into both directions. This supports the notion that under- and overshooting imply different consequences for postsaccadic oculomotor processing. Results are discussed using a model, introduced by Rolfs, Kliegl, and Engbert (2008), to account for the generation of microsaccades. We argue that the dynamic interplay of target eccentricity and the magnitude of the saccadic under-/overshoot can be explained by a different strength of activation in the two hemispheres of the saccadic motor map in this model.
There has been a substantial increase in the percentage for publications with co-authors located in departments from different countries in 12 major journals of psychology. The results are evidence for a remarkable internationalization of psychological research, starting in the mid 1970s and increasing in rate at the beginning of the 1990s. This growth occurs against a constant number of articles with authors from the same country; it is not due to a concomitant increase in the number of co-authors per article. Thus, international collaboration in psychology is obviously on the rise.
In alphabetic writing systems, saccade amplitude (a close correlate of reading speed) is independent of font size, presumably because an increase in the angular size of letters is compensated for by a decrease of visual acuity with eccentricity. We propose that this invariance may (also) be due to the presence of spaces between words, guiding the eyes across a large range of font sizes. Here, we test whether saccade amplitude is also invariant against manipulations of font size during reading Chinese, a character-based writing system without spaces as explicit word boundaries for saccade-target selection. In contrast to word-spaced alphabetic writing systems, saccade amplitude decreased significantly with increased font size, leading to an increase in the number of fixations at the beginning of words and in the number of refixations. These results are consistent with a model which assumes that word beginning (rather than word center) is the default saccade target if the length of the parafoveal word is not available.
The lexical database dlexDB supplies in form of an online database frequency-based norms of numerous process-related word properties for psychological and linguistic research. These values include well known variables such as printed frequency of word form and lemma as documented also in CELEX (Baayen, Piepenbrock und Gulikers, 1995). In addition, we compute new values like frequencies based on syllables, and morphemes as well as frequencies of character chains, and multiple word combinations. The statistics are based on the Kernkorpus des Digitalen Wrterbuchs der deutschen Sprache (DWDS) with over 100 million running words. We illustrate the validity of these norms with new results about fixation durations in sentence reading.
Eye fixation durations during normal reading correlate with processing difficulty, but the specific cognitive mechanisms reflected in these measures are not well understood. This study finds support in German readers' eye fixations for two distinct difficulty metrics: surprisal, which reflects the change in probabilities across syntactic analyses as new words are integrated; and retrieval, which quantifies comprehension difficulty in terms of working memory constraints. We examine the predictions of both metrics using a family of dependency parsers indexed by an upper limit on the number of candidate syntactic analyses they retain at successive words. Surprisal models all fixation measures and regression probability. By contrast, retrieval does not model any measure in serial processing. As more candidate analyses are considered in parallel at each word, retrieval can account for the same measures as surprisal. This pattern suggests an important role for ranked parallelism in theories of sentence comprehension.
We determined experimentally the complex transient optical dielectric function of a well-characterized polyelectrolyte/gold-nanoparticle composite system over a broad spectral range upon short pulse laser excitation by simultaneously measuring the time-dependent reflectance and transmittance of white light pulses with femtosecond pump-probe spectroscopy. We extracted directly the ultrafast changes in the real and imaginary parts of the effective dielectric function, epsilon(eff)(r) (omega,t)and epsilon(eff)(i) (omega,t), from the experiment. This complete experimental set of information on the time-dependent complex dielectric function challenges theories modeling the transient dielectric function of gold particles and the effective medium.
Identifying the chemical mechanisms behind soil carbon bound in organo-mineral complexes is necessary to determine the degree to which soil organic carbon is stabilized belowground. Analysis of delta C-13 and delta N-15 isotopic signatures of stabilized OM fractions along with soil mineral characteristics may yield important information about OM-mineral associations and their processing history. We anlayzed the delta C-13 and delta N-15 isotopic signatures from two organic matter (OM) fractions along with soil mineral proxies to identify the likely binding mechanisms involved. We analyzed OM fractions hypothesized to contain carbon stabilized through organo-mineral complexes: (1) OM separated chemically with sodium pyrophosphate (OM(PY)) and (2) OM occluded in micro-structures found in the chemical extraction residue (OM(ER)). Because the OM fractions were separated from five different soils with paired forest and arable land use histories, we could address the impact of land use change on carbon binding and processing mechanisms. We used partial least squares regression to analyze patterns in the isotopic signature of OM with established mineral and chemical proxies indicative for certain binding mechanisms. We found different mechanisms predominate in each land use type. For arable soils, the formation of OM(PY)-Ca-mineral associations was identified as an important OM binding mechanism. Therefore, we hypothesize an increased stabilization of microbial processed OM(PY) through Ca2+ interactions. In general, we found the forest soils to contain on average 10% more stabilized carbon relative to total carbon stocks, than the agricultural counter part. In forest soils, we found a positive relationship between isotopic signatures of OM(PY) and the ratio of soil organic carbon content to soil surface area (SOC/SSA). This indicates that the OM(PY) fractions of forest soils represent layers of slower exchange not directly attached to mineral surfaces. From the isotopic composition of the OM(ER) fraction, we conclude that the OM in this fraction from both land use types have undergone a different pathway to stabilization that does not involve microbial processing, which may include OM which is highly protected within soil micro-structures.
Ground-based gamma-ray astronomy has had a major breakthrough with the impressive results obtained using systems of imaging atmospheric Cherenkov telescopes. Ground-based gamma-ray astronomy has a huge potential in astrophysics, particle physics and cosmology. CTA is an international initiative to build the next generation instrument, with a factor of 5-10 improvement in sensitivity in the 100 GeV-10 TeV range and the extension to energies well below 100 GeV and above 100 TeV. CTA will consist of two arrays (one in the north, one in the south) for full sky coverage and will be operated as open observatory. The design of CTA is based on currently available technology. This document reports on the status and presents the major design concepts of CTA.
Editorial
(2011)
Inhalt: Editorial (Deutsch) Editorial (Englisch)
Vom Raum zur Geschichte
(2011)
Der Begriff des Ghettos hat eine lange Forschungsgeschichte. Dieser Beitrag diskutiert ihn im Kontext der Historisierung der Lebenswelten des 19. Jahrhunderts. Der Terminus wird im 19. Jahrhundert zum Inbegriff der frühneuzeitlichen Tradition. Im Folgenden werden einige Aspekte der Entstehungsgeschichte dieses Bedeutungswandels analysiert, der auf einer Zivilisierungsdynamik gegenüber den Juden im ausgehenden 18. Jahrhundert wie der Historisierung der Hebräischen Bibel in der Aufklärung basierte.
Lebenswelt Ghetto
(2011)
Der Aufsatz zeigt, inwiefern raumtheoretische Ansätze mit dem interpretativen Paradigma verbunden werden können und so einen Zugang zu den durch die nationalsozialistische Ghettoisierung hervorgerufenen Veränderungen im sozialen Handeln der Ghettobewohner ermöglichen. Es wird argumentiert, dass die Raumtheorie hier zu kurz greift und durch interpretative Ansätze ergänzt werden muss, um den sozialen Phänomenen im Ghetto gerecht zu werden. Die beiden Ansätze werden in ihrer Bedeutung für die Erforschung der Ghettogesellschaften dargestellt und an zwei Beispielen, „Kriminalität“ und Bildung, in ihrer Anwendbarkeit vorgeführt.