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Keywords
- Vitamin A (6)
- HPLC (5)
- vitamin A (5)
- retinol (4)
- RBP4 (3)
- iCheck (3)
- retinol-binding protein 4 (3)
- Bioavailability (2)
- Biomarker (2)
- Dogs (2)
Effect of ß-carotene feeding on vitamin A and retinol-binding protein in the uterine fluid of pigs
(1997)
Verteilung von Vitamin A und Retinol-Bindungs-Protein (RBP) in verschiedenen Geweben von Caniden
(1997)
Effect of age on the levels of retinol and retinyl ester in blood plasma, liver and kidney of dogs
(1998)
Vitamin A excreted in the urine of canines is associated with a Tamm-Horsfall-like Glycoprotein
(1998)
Effect of parturition on levels of vitamins A and E and of ß-carotene in plasma and milk of mares
(1999)
Physiologie der Laktation
(2000)
Vitamine
(2000)
Untersuchungen zum Transfer von Carotinoiden aus dem Plasma in die Follikelflüssigkeit der Frau
(2000)
Veränderung der Carotinoidkonzentration im Serum und in der Milch im Verlauf der Laktation der Frau
(2000)
Transfer of carotenoids, alfa-tocopherol and retinol from plasma into follicular fluid in women
(2000)
Retinoid- and carotenoid-enriched diets influence the ontogenesis of the immune system in mice
(2003)
Carotinoide
(2003)
In this study, a method for partly automated sample preparation and fully automated solid-phase extraction method for plasma, kidney and liver samples for various retinoids like all-trans-4-oxo-retinoic acid, 13-cis-4-oxo- retinoic acid, 13-cis-retinoic acid, 9-cis-retinoic acid, all-trans-retinoic acid, retinol and retinyl palmitate was established. Plasma, embryo-, kidney-and liver-homogenates were automatically mixed and extracted on multiple usage solid-phase (C2) extraction cartridges immediately before HPLC analysis. Automated cleaning, preconditioning and incorporation of the loaded cartridge to fully automated HPLC separation and quantification of the various retinoids in a single HPLC run was established. The recovery of the retinoids was generally between 80 and 90%. Intra-day repeatability was <11.7%. As little as 1.2 ng/ml could be quantified in lipid-mixture standard samples. This method allows a highly automated sample preparation and a fully automated solid-phase extraction with good selectivity for the study of endogenous retinoids and retinoids after nutritional supplementations and pharmacological applications in several biological samples. (C) 2003 Elsevier B.V. All rights reserved