Refine
Has Fulltext
- no (655) (remove)
Year of publication
- 2024 (3)
- 2023 (28)
- 2022 (16)
- 2021 (12)
- 2020 (14)
- 2019 (32)
- 2018 (20)
- 2017 (38)
- 2016 (45)
- 2015 (37)
- 2014 (22)
- 2013 (36)
- 2012 (41)
- 2011 (35)
- 2010 (31)
- 2009 (37)
- 2008 (42)
- 2007 (35)
- 2006 (17)
- 2005 (19)
- 2004 (6)
- 2003 (13)
- 2002 (7)
- 2001 (17)
- 2000 (11)
- 1999 (8)
- 1998 (11)
- 1997 (4)
- 1996 (1)
- 1995 (1)
- 1994 (3)
- 1993 (3)
- 1992 (3)
Document Type
- Doctoral Thesis (655) (remove)
Keywords
- photosynthesis (5)
- Arabidopsis thaliana (4)
- Photosynthese (3)
- fluctuating light (3)
- plant (3)
- E. coli (2)
- Naturschutz (2)
- adaptation (2)
- ancient DNA (2)
- atomic force microscopy (2)
- cell shape (2)
- cell wall (2)
- conservation (2)
- evolution (2)
- genomics (2)
- metabolism (2)
- metabolomics (2)
- movement ecology (2)
- plastid transformation (2)
- shoot apical meristem (2)
- (Xeno)Hormone (1)
- (xeno)hormones (1)
- 13CO2 labeling (1)
- ABC Transporter (1)
- ABC transporter (1)
- ABCB7 (1)
- AIDS (1)
- AMP (1)
- APX2 (1)
- African weakly electric fish (1)
- Aktinzytoskelett (1)
- Aktivität (1)
- Aldehyd (1)
- Aldehyd-oxidase (1)
- Aldehydoxidase (1)
- Amphibians (1)
- Amphibien (1)
- Annamites (1)
- Anpassung (1)
- Antibody (1)
- Arabidopsis (1)
- Assemblierungsfaktor (1)
- Auxin (1)
- B. calyciflorus species complex (1)
- Bakterien (1)
- Bakteriophagen (1)
- Bewegungsökologie (1)
- Bildanalyse (1)
- Biofilm (1)
- Biogenese (1)
- Biomarker (1)
- Bombina bombina (1)
- Brachionus (1)
- Brassinosteriods (1)
- Chlamydomonas (1)
- Cylindrospermopsis raciborskii (1)
- DEK1 (1)
- DNA (1)
- DNA Methylation (1)
- DNA metabarcoding (1)
- DON (1)
- Darmkrebs (1)
- Deakklimatisierung (1)
- Differenzielle Genexpression (1)
- Durchflusszytometrie (1)
- Early Starvation 1 (1)
- Einzelzell-Analyse (1)
- Einzelzell-Sequenzierung (1)
- Enzymkinetik (1)
- Epigenetics (1)
- Epigenetik (1)
- Eschericha coli (1)
- Escherichia coli (1)
- European bats (1)
- Europäische Fledermausarten (1)
- Evolution (1)
- F-box (1)
- F. oxysporum (1)
- FLC (1)
- FLOWERING LOCUS C (1)
- Fisch (1)
- Formgedächtnispolymere (1)
- Fortbewegung (1)
- G protein-coupled estrogen receptor (1)
- G protein-gekoppelter Östrogen Rezeptor (1)
- GTPase (1)
- Genetic rescue (1)
- Genetische Rettung (1)
- Genomik (1)
- Griffithsin (1)
- Growth signaling (1)
- H3K27me3 Methylation (1)
- H3K9ac (1)
- HAC1 (1)
- HIV (1)
- HS transcriptional memory (1)
- HS-Transkriptionsgedächtnis (1)
- HSE (1)
- HSFA2 (1)
- Haptene (1)
- Heat stress memory, Heat shock factors, Mediator subunits, MED23, MED32, Arabidopsis thaliana (1)
- Histone Modification (1)
- HopZ1a (1)
- Hybridisierung (1)
- Hybridom (1)
- Hypoxie (1)
- IDPs (1)
- Immunfluoreszenz (1)
- Introgression (1)
- Ionentransport (1)
- Isoenzyme (1)
- JUMONJI (1)
- Koexpression Netzwerk Analysen (1)
- Kompromiss (1)
- Konfokale Mikroskopie (1)
- Krankheitsausbruch (1)
- Krankheitsökologie (1)
- Kältestress (1)
- LC-OCD-OND (1)
- LEA (1)
- LEUNIG LEUNIG_HOMOLOG SEUSS SEUSS-LIKEs Arabidopsis embryogenesis WOX2-module HD-ZIPIII (1)
- Landschaftsanalyse (1)
- Landschaftsheterogenität (1)
- Lichtverschmutzung (1)
- Lipopolysaccharid (1)
- MADS (1)
- Maschinelles-Lernen (1)
- Matrix (1)
- Metabolic Engineering (1)
- Metabolismus von Medikamenten (1)
- Metabolomics (1)
- Metacommunity (1)
- Metagemeinschaft (1)
- Metallkation (1)
- Mitochondrien (1)
- Molybdän (1)
- Molybdänkofaktor (1)
- Multiple-Sklerose (1)
- Muskel-Skelett-System / Bewegungsapparat (1)
- Naturschutzgenetik (1)
- Netzwerke (1)
- Neuroendocrine tumors (1)
- Nicotiana tabacum (1)
- Oxidoreduktase (1)
- PBS1 (1)
- PGR5 (1)
- PLP-Walker A-overlap (1)
- PLP-Walker A-Überlagerung (1)
- Palmöl (1)
- Parkinson's disease (1)
- Parkinson-Krankheit (1)
- Periplasma (1)
- Pflanze (1)
- Pflanzenzellen (1)
- Phosphoproteomik (1)
- Photorespiration (1)
- Photosystem I (1)
- Pichia pastoris (1)
- Populationsgenetik (1)
- Pseudomonas syringae (1)
- R (Programmiersprache) (1)
- RNA-Sequenzierung (1)
- RNA-seq (1)
- RNA-sequencing (1)
- Reaktionsgeschwindigkeit (1)
- Remorin (1)
- Rhodobacter capsulatus (1)
- Rstats (1)
- Rubisco (1)
- SAP (1)
- SCF complex (1)
- STERILE APETALA (1)
- SUMO (1)
- SVP (1)
- Seed Coat Development (1)
- Seed development (1)
- Solanum tuberosum (1)
- Source und Sink (1)
- Stammzelldifferenzierung (1)
- Stoffwechsel (1)
- Stärke (1)
- Synthetic Biology (1)
- Target of Rapamycin kinase (1)
- Theranostic (1)
- Thylakoid (1)
- Thylakoidmembran (1)
- Transkriptionsfaktoren (1)
- Transkriptomik (1)
- UDP-glucose (1)
- Urbanisation (1)
- V. dahliae (1)
- Vesikel (1)
- Viskoelastizität (1)
- WD40 (1)
- WGCNA (1)
- Wachstumssignale (1)
- Wildschwein (1)
- Y chromosome (1)
- Zahnwale (1)
- Zellform (1)
- Zellmembran (1)
- Zentrosomen Amplifikation (1)
- Zooplankton (1)
- actin (1)
- actin cytoskeleton machine (1)
- activity (1)
- adaptive radiation (1)
- agent-based model (1)
- agentenbasiertes Modell (1)
- aldehyde oxidase (1)
- alpha-helix (1)
- animal (1)
- animal personality (1)
- anthropogene Umweltveränderungen (1)
- anti bacterial (1)
- antimicrobial peptide (1)
- antiviral agent (1)
- assembly factor (1)
- ausgewogener Komplex (1)
- bacteria (1)
- bacteriophage (1)
- balanced complex (1)
- bifunctional enzyme (1)
- biofilm (1)
- biogenesis (1)
- biomarker (1)
- biomechanics (1)
- camera-trap (1)
- cbFBA (1)
- cell division (1)
- cell membrane (1)
- cell morphogenesis (1)
- cells epidermis (1)
- cellulose (1)
- cellulose biosynthesis inhibitor (1)
- cellulose microfibrils (1)
- cellulose synthase complex (1)
- cellulose synthesis (1)
- centrosome amplification (1)
- chloro-ribosome (1)
- chloroplast (1)
- chromosomale Instabilität (1)
- classical swine fever (1)
- co-expression network analysis (1)
- coat colour (1)
- coil-helix (1)
- cold stress (1)
- colon cancer (1)
- compliant mechanism (1)
- confocal microscopy (1)
- conservation genetics (1)
- constraint-based modeling (1)
- conventional agriculture (1)
- cyanobacteria (1)
- deacclimation (1)
- differential gene expression (1)
- disease ecology (1)
- disease persistence (1)
- domestication (1)
- drug metabolism (1)
- ecological interactions (1)
- ecological speciation (1)
- editing (1)
- endosymbiosis (1)
- energy metabolism (1)
- enzyme isoforms (1)
- enzyme kinetics (1)
- epigenetics (1)
- equus caballus (1)
- fff (1)
- fish (1)
- flow cytometry (1)
- flower development (1)
- flowering (1)
- fluktuierendes Licht (1)
- formate assimilation (1)
- formate dehydrogenases (1)
- fusion (1)
- galactolipids (1)
- gastric inhibitory polypeptide receptor (1)
- gene (1)
- genome (1)
- genome-wide association studies (GWAS) (1)
- genotype-by-Environmental interaction (GxE) (1)
- geometric morphometric (1)
- gram-negative (1)
- gramnegativ (1)
- hapten (1)
- herbicide (1)
- horse (1)
- human-induced rapid environmental change (1)
- hybridization (1)
- hybridoma (1)
- hypoxia (1)
- iatom diversity (1)
- immunofluorescence (1)
- inducible expression (1)
- inter-individual differences (1)
- invasion (1)
- ion transport (1)
- klassische Schweinepest (1)
- konventionelle Landwirtschaft (1)
- landscape analysis (1)
- landscape heterogeneity (1)
- learning networks plant (1)
- light pollution (1)
- linker (1)
- lipopolysaccharide (1)
- locomotion (1)
- low NPQ (1)
- machine-learning (1)
- matrix (1)
- mesenchymal stromal cells (1)
- mesenchymale stromale Zellen (1)
- metabarcoding (1)
- metabolic engineering (1)
- metabolic network (1)
- metabolic networks (1)
- metabolisches Netzwerk (1)
- metal cation (1)
- methanol assimilation (1)
- micorbicide (1)
- mitochondria (1)
- mitochondrial transformation (1)
- molecular farming (1)
- molybdenum cofactor (1)
- monoclonal antibodies (1)
- monoklonale Antikörper (1)
- morphogenesis (1)
- movement (1)
- multiple sclerosis (1)
- musculoskeletal system (1)
- natural organic matter (1)
- neurodegeneration (1)
- neuroinflammation (1)
- occupancy (1)
- oil palm (1)
- organ size (1)
- organelles (1)
- pavement cells image analysis (1)
- peptide (1)
- pflanzliches Immunsystem (1)
- phenomics (1)
- phosphoglucan (1)
- phosphoproteomics (1)
- photosystem I (1)
- phyllotaxis (1)
- phylogenetic diversity (1)
- phytoplankton (1)
- plant cell wall (1)
- plant diversity (1)
- plant immune system (1)
- plant-microbe interaction (1)
- plasticity (1)
- polypeptide (1)
- population (1)
- population genetics (1)
- potato (1)
- proteasomal degradation (1)
- protein fusion (1)
- protein-folding (1)
- reaction rate (1)
- recombinant production (1)
- repair (1)
- ribosome assembly (1)
- root growth (1)
- root microbiota (1)
- root volatiles (1)
- schwach elektrische Fische (1)
- schwankendes Licht (1)
- shape-memory polymer (1)
- shape-memory polymers (1)
- short vegetative phase (1)
- single-cell RNA-sequencing (1)
- single-cell analysis (1)
- soft actuators (1)
- source and sink (1)
- space use (1)
- starch (1)
- starch granule surface (1)
- starch phosphorylation (1)
- stem cell differentiation (1)
- structure-function (1)
- sulfadiazine (1)
- suppressor mutant screen (1)
- targeted therapy (1)
- temperature (1)
- thermoplastic polymers (1)
- thermoplastischen Polymere (1)
- threatened (1)
- thylakoid (1)
- thylakoid membranes (1)
- tobacco (1)
- tobramycin (1)
- tomato(Solanum lycopersicum) (1)
- toothed whales (1)
- trade-off (1)
- transcription factors (1)
- transcriptome (1)
- transcriptomics (1)
- transfer (1)
- transgenic (1)
- translation (1)
- trophic apparatus (1)
- tropical freshwater fish (1)
- tropische Süßwasser Fische (1)
- tuber second growth (1)
- tuberization (1)
- ubiquitin (1)
- ubiquitination (1)
- urbanization (1)
- vesicle (1)
- viscoelasticity (1)
- weakly electric fish (1)
- weiche Aktuatoren (1)
- whole chromosomal instability (1)
- whole genome (1)
- wild boar (1)
- zooplankton (1)
Institute
- Institut für Biochemie und Biologie (655) (remove)
Einfluss von Intraguild Predation auf die Dynamik der Planktonsukzession in einem sauren Bergbausee
(2005)
Investigation of the TCA cycle and glycolytic metabolons and their physiological impacts in plants
(2016)
Electrosynthesis and characterization of molecularly imprinted polymers for peptides and proteins
(2019)
Rolle der GTPase ARFRP1 für die Golgi-Funktion und die Differenzierung epithelialer Zellen des Darms
(2007)
The horse is a fascinating animal symbolizing power, beauty, strength and grace. Among all the animal species domesticated the horse had the largest impact on the course of human history due to its importance for warfare and transportation. Studying the process of horse domestication contributes to the knowledge about the history of horses and even of our own species.
Research based on molecular methods has increasingly focused on the genetic basis of horse domestication. Mitochondrial DNA (mtDNA) analyses of modern and ancient horses detected immense maternal diversity, probably due to many mares that contributed to the domestic population. However, mtDNA does not provide an informative phylogeographic structure. In contrast, Y chromosome analyses displayed almost complete uniformity in modern stallions but relatively high diversity in a few ancient horses. Further molecular markers that seem to be well suited to infer the domestication history of horses or genetic and phenotypic changes during this process are loci associated with phenotypic traits.
This doctoral thesis consists of three different parts for which I analyzed various single nucleotide polymorphisms (SNPs) associated with coat color, locomotion or Y chromosomal variation of horses. These SNPs were genotyped in 350 ancient horses from the Chalcolithic (5,000 BC) to the Middle Ages (11th century). The distribution of the samples ranges from China to the Iberian Peninsula and Iceland. By applying multiplexed next-generation sequencing (NGS) I sequenced short amplicons covering the relevant positions: i) eight coat-color-associated mutations in six genes to deduce the coat color phenotype; ii) the so-called ’Gait-keeper’ SNP in the DMRT3 gene to screen for the ability to amble; iii) 16 SNPs previously detected in ancient horses to infer the corresponding haplotype. Based on these data I investigated the occurrence and frequencies of alleles underlying the respective phenotypes as well as Y chromosome haplotypes at different times and regions. Also, selection coefficients for several Y chromosome lineages or phenotypes were estimated.
Concerning coat color differences in ancient horses my work constitutes the most comprehensive study to date. I detected an increase of chestnut horses in the Middle Ages as well as differential selection for spotted and solid phenotypes over time which reflects changing human preferences.
With regard to ambling horses, the corresponding allele was present in medieval English and Icelandic horses. Based on these results I argue that Norse settlers, who frequently invaded parts of Britain, brought ambling individuals to Iceland from the British Isles which can be regarded the origin of this trait. Moreover, these settlers appear to have selected for ambling in Icelandic horses.
Relating to the third trait, the paternal diversity, these findings represent the largest ancient dataset of Y chromosome variation in non-humans. I proved the existence of several Y chromosome haplotypes in early domestic horses. The decline of Y chromosome variation coincides with the movement of nomadic peoples from the Eurasian steppes and later with different breeding practices in the Roman period.
In conclusion, positive selection was estimated for several phenotypes/lineages
in different regions or times which indicates that these were preferred by humans. Furthermore, I could successfully infer the distribution and dispersal of horses in association with human movements and actions. Thereby, a better understanding of the influence of people on the changing appearance and genetic diversity of domestic horses could be gained. My results also emphasize the close relationship of ancient genetics and archeology or history and that only in combination well-founded conclusions can be reached.
Exploring the Arabidopsis metabolic landscape by genetic mapping integrated with network analysis
(2017)
To meet the demands of a growing world population while reducing carbon dioxide (CO2) emissions, it is necessary to capture CO2 and convert it into value-added compounds. In recent years, metabolic engineering of microbes has gained strong momentum as a strategy for the production of valuable chemicals. As common microbial feedstocks like glucose directly compete with human consumption, the one carbon (C1) compound formate was suggested as an alternative feedstock. Formate can be easily produced by various means including electrochemical reduction of CO2 and could serve as a feedstock for microbial production, hence presenting a novel entry point for CO2 to the biosphere and a storage option for excess electricity. Compared to the gaseous molecule CO2, formate is a highly soluble compound that can be easily handled and stored. It can serve as a carbon and energy source for natural formatotrophs, but these microbes are difficult to cultivate and engineer. In this work, I present the results of several projects that aim to establish efficient formatotrophic growth of E. coli – which cannot naturally grow on formate – via synthetic formate assimilation pathways. In the first study, I establish a workflow for growth-coupled metabolic engineering of E. coli. I demonstrate this approach by presenting an engineering scheme for the PFL-threonine cycle, a synthetic pathway for anaerobic formate assimilation in E. coli. The described methods are intended to create a standardized toolbox for engineers that aim to establish novel metabolic routes in E. coli and related organisms. The second chapter presents a study on the catalytic efficiency of C1-oxidizing enzymes in vivo. As formatotrophic growth requires generation of both energy and biomass from formate, the engineered E. coli strains need to be equipped with a highly efficient formate dehydrogenase, which provides reduction equivalents and ATP for formate assimilation. I engineered a strain that cannot generate reducing power and energy for cellular growth, when fed on acetate. Under this condition, the strain depends on the introduction of an enzymatic system for NADH regeneration, which could further produce ATP via oxidative phosphorylation. I show that the strain presents a valuable testing platform for C1-oxidizing enzymes by testing different NAD-dependent formate and methanol dehydrogenases in the energy auxotroph strain. Using this platform, several candidate enzymes with high in vivo activity, were identified and characterized as potential energy-generating systems for synthetic formatotrophic or methylotrophic growth in E. coli. In the third chapter, I present the establishment of the serine threonine cycle (STC) – a synthetic formate assimilation pathway – in E. coli. In this pathway, formate is assimilated via formate tetrahydrofolate ligase (FtfL) from Methylobacterium extorquens (M. extorquens). The carbon from formate is attached to glycine to produce serine, which is converted into pyruvate entering central metabolism. Via the natural threonine synthesis and cleavage route, glycine is regenerated and acetyl-CoA is produced as the pathway product. I engineered several selection strains that depend on different STC modules for growth and determined key enzymes that enable high flux through threonine synthesis and cleavage. I could show that expression of an auxiliary formate dehydrogenase was required to achieve growth via threonine synthesis and cleavage on pyruvate. By overexpressing most of the pathway enzymes from the genome, and applying adaptive laboratory evolution, growth on glycine and formate was achieved, indicating the activity of the complete cycle. The fourth chapter shows the establishment of the reductive glycine pathway (rGP) – a short, linear formate assimilation route – in E. coli. As in the STC, formate is assimilated via M. extorquens FtfL. The C1 from formate is condensed with CO2 via the reverse reaction of the glycine cleavage system to produce glycine. Another carbon from formate is attached to glycine to form serine, which is assimilated into central metabolism via pyruvate. The engineered E. coli strain, expressing most of the pathway genes from the genome, can grow via the rGP with formate or methanol as a sole carbon and energy source.