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Regulation alters the characteristics of riversty transforming parts of them into lakes, affecting their hydrology and also the physical, chemical, and biological characteristics and dynamics. Reservoirs have proven to be very effective retaining particulate materials, thereby avoiding the downstream transport of suspended sediment and the chemical substances associated with it (e.g. Carbon, C or Nitrogen, N). The study of fluvial transport of C and N is of great interest since river load represents a major link to the global C and N cycles. Moreover, reservoirs are the most important sinks for organic carbon among inland waters and have a potential significance as nitrogen sinks. In this respect, this paper investigates the effects of a Pyrenean reservoir on the runoff, suspended sediment, C and N derived from the highly active Esera and Isabena rivets. Key findings indicate that the reservoir causes a considerable impact on the Esera-Isabena river fluxes, reducing them dramatically as almost all the inputs are retained within the reservoir. Despite the very dry study year (2011-2012), it can be calculated that almost 300,000 t of suspended sediment were deposited into the Barasona Reservoir, from which more than 16,000 were C (i.e. 2200 t as organic C) and 222 t were N. These values may not be seen as remarkable in a wider global context but, assuming that around 30 hm(3) of sediment are currently stored in the reservoir, figures would increase up to ca. 2.6 x 10(6) t of C (i.e. 360,000 t of organic C) and 35,000 t of N. Nevertheless, these values are indicative and should be treated with caution as there is incomplete understanding of all the processes which affect C and N. Further investigation to establish a more complete picture of C and N yields and budgets by monitoring the different processes involved is essential. (C) 2015 Elsevier B.V. All rights reserved.
Mediterranean climate is characterized by highly irregular rainfall patterns with marked differences between wet and dry seasons which lead to highly variable hydrological fluvial regimes. As a result, and in order to ensure water availability and reduce its temporal variability, a high number of large dams were built during the 20th century (more than 3500 located in Mediterranean rivers). Dams modify the flow regime but also interrupt the continuity of sediment transfer along the river network, thereby changing its functioning as an ecosystem. Within this context, the present paper aims to assess the suspended sediment loads and dynamics of two climatically contrasting Mediterranean regulated rivers (i.e. the Esera and Siurana) during a 2-yr period. Key findings indicate that floods were responsible for 92% of the total suspended sediment load in the River Siurana, while this percentage falls to 70% for the Esera, indicating the importance of baseflows on sediment transport in this river. This fact is related to the high sediment availability, with the Esera acting as a non-supply-limited catchment due to the high productivity of the sources (i.e. badlands). In contrast, the Siurana can be considered a supply-limited system due to its low geomorphic activity and reduced sediment availability, with suspended sediment concentration remaining low even for high magnitude flood events. Reservoirs in both rivers reduce sediment load up to 90%, although total runoff is only reduced in the case of the River Esera. A remarkable fact is the change of the hydrological character of the River Lem downstream for the dam, shifting from a humid mountainous river regime to a quasi-invariable pattern, whereas the Siurana experiences the opposite effect, changing from a flashy Mediterranean river to a more constant flow regime below the dam. (C) 2015 Elsevier B.V. All rights reserved.
EPR spectroscopy is a well suited analytical tool to monitor the electronic situation around paramagnetic metal centres as copper(II) and therefore the structural influences on the paramagnetic ion. 1,2-Dithiosquaratometalates are available by direct synthesis from metal salts with dipotassium-1,2-dithiosquarate and the appropriate counter cations. Synthesis and characterisation of bis(benzyltributylammonium)1,2-dithiosquaratonickelate(II), (BzlBu(3)N)(2)[Ni(dtsq)(2)], and bis(benzyltributylammonium)1,2-dithiosquaratocuprate(II), (BzlBu(3)N)(2)[Cu(dtsq)(2)], with benzyltributylammonium as the counter ion is reported and the X-ray structures of two complexes, (BzlBu(3)N)(2)[Ni(dtsq)(2)] and (BzlBu(3)N)(2)[Cu(dtsq)(2)], are presented. Both complexes, crystallising in the monoclinic space group P2(1)/c, are isostructural with only small differences in the coordination sphere due to the different metal ions. The diamagnetic nickel complex is therefore well suited as a host lattice for the paramagnetic Cu(II) complex to measure EPR for additional structural information. (c) 2015 Institute of Chemistry, Slovak Academy of Sciences
We construct equivariant KK-theory with coefficients in and R/Z as suitable inductive limits over II1-factors. We show that the Kasparov product, together with its usual functorial properties, extends to KK-theory with real coefficients. Let Gamma be a group. We define a Gamma-algebra A to be K-theoretically free and proper (KFP) if the group trace tr of Gamma acts as the unit element in KKR Gamma (A, A). We show that free and proper Gamma-algebras (in the sense of Kasparov) have the (KFP) property. Moreover, if Gamma is torsion free and satisfies the KK Gamma-form of the Baum-Connes conjecture, then every Gamma-algebra satisfies (KFP). If alpha : Gamma -> U-n is a unitary representation and A satisfies property (KFP), we construct in a canonical way a rho class rho(A)(alpha) is an element of KKR/Z1,Gamma (A A) This construction generalizes the Atiyah-Patodi-Singer K-theory class with R/Z-coefficients associated to alpha. (C) 2015 Elsevier Inc. All rights reserved.
The uppermost Cretaceous (upper Campanian-Maastrichtian) marine deposits of the central south Pyrenees host a rich larger benthic foraminiferal fauna and several rudist-rich levels. These marine deposits are directly overlain by the continental facies of the Aren and Tremp Formations, which are famous for their fossil dinosaur remains. Larger benthic foraminiferal distribution documents an important faunal turnover in all the carbonate platform environments within the photic zone, from open marine to littoral areas. Biostratigraphy indicates that this turnover occurred close to the Campanian-Maastrichtian boundary. This is also confirmed by strontium isotope stratigraphy which indicates an earliest Maastrichtian age for the appearance of the larger benthic foraminiferal assemblage constituted by Lepidorbitoides socialis, Clypeorbis mammillata, Wannierina cataluniensis, Orbitoides gruenbachensis, Siderolites aff. calcitrapoides, Fascispira colomi, Omphalocyclus macroporus and Laffiteina mengaudi. In particular, a numerical age of 71 Ma is obtained for the Hippurites radiosus level, just a few meters below the first continental deposits of the Aren sensu stricto Formation. The youngest marine sediments of the central south Pyrenees are early Maastrichtian in age. This is also an important constraint for the age of the end-Cretaceous dinosaur fossil localities of the Tremp basin. (C) 2015 Elsevier Ltd. All rights reserved.
Sarmentofascis zamparelliae n. sp., a new demosponge from the lower Campanian of southern Italy
(2016)
A new coralline sponge, exhibiting typical "stromatoporoid" bodyplan, is described as Sarmentofascis zamparelliae n. sp. from the lower Campanian of the southern Apennines, Italy. It is differentiated from Sarmentofascis cretacea (Turnsek) (Hauterivian of Montenegro) and Sarmentofascis chabrieri Termier, Termier and Vachard (Santonian of France) above all by its slender arborescent skeleton, exhibiting longitudinally distributed astrorhizae-like canals. S. zamparelliae n. sp. is the youngest representative of the genus and is reported from a period exhibiting a distinct decline of "stromatoporoid" sponges. With its clinogonal microstructure and occurrence in inner platform stromatoporoid-foraminiferan floatstones it can be considered a Late Cretaceous environmental analog to the Late Jurassic Cladocoropsis. (C) 2015 Elsevier Ltd. All rights reserved.
The water cycle of sites with shallow groundwater tables is characterized by complex interactions of hydrological and ecological processes. The water balance components, which are subject to diurnal fluctuations, are best measured with groundwater lysimeters. However, the lower boundary condition of such lysimeters affects most of the hydrological variables, particularly when considering short time scales, and has to be defined in such a way as to facilitate realistic simulations. In this paper, different means of controlling the lower boundary condition of groundwater lysimeters were compared with respect to their ability to simulate the behavior of the water balance components properly. Measurements of rain-free periods from a lysimeter station installed in the Spreewald wetland in north-east Germany were evaluated. The most common groundwater lysimeter type is controlled using a Mariotte bottle and sets the groundwater level in the soil monolith to a constant level, which here caused an alteration of the inflow to the lysimeter, with respect to both its value and diurnal behavior. Still, daily evapotranspiration values were realistic and this simple and robust approach may be used for time intervals not shorter than one day. High-resolution measurements can be gained from lysimeters that automatically adjust the groundwater level by a system of pumps and valves on an hourly basis. Still, reliable results were only obtained when the conditions in the lysimeter and the surrounding field, where the target groundwater level was measured, were in accordance. Otherwise (e.g., when the groundwater level differed) an unrealistic inflow behavior evolved. Reasonable results, even for slightly diverging conditions, were gained with a new approach that defined the lower boundary conditions by controlling the inflows and outflows of the lysimeter. This approach further enabled the groundwater level itself to be the study subject, thereby enlarging the field of possible applications of groundwater lysimeters. (C) 2015 Elsevier B.V. All rights reserved.
Socio-political studies in mathematics education often touch complex fields of interaction between education, mathematics and the political. In this paper I present a Foucault-based framework for socio-political studies in mathematics education which may guide research in that area. In order to show the potential of such a framework, I discuss the potential and limits of Marxian ideology critique, present existing Foucault-based research on socio-political aspects of mathematics education, develop my framework and show its use in an outline of a study on socio-political aspects of calculation in the mathematics classroom.
The electrophoretic deposition process was used to produce multi-layered ceramics consisting of alternating layers of fully stabilized cubic zirconia and partially stabilized tetragonal zirconia to make use of their different mechanical behaviour, investigating the possibility to deflect advancing cracks at the interfaces of the different layers. This crack deflection is apparently impacted by a toughening mechanism only found in the tetragonal stabilized zirconia polymorph and is characterized by the stress induced transformation of the metastable tetragonal phase into the monoclinic one, which is accompanied by a volume increase resulting in a closing mechanism for advancing cracks. While improving the electrophoretic deposition process, we investigated the transformation toughening mechanism at the layer interfaces and their effect on crack propagation. Investigations involved a combination of different imaging methods, including light microscopy, white light interferometry, atomic force microscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy and Raman spectroscopy. (C) 2015 Elsevier Ltd. All rights reserved.
Nojima, Hiroyuki ; Freeman, Christopher M. ; Schuster, Rebecca M. ; Japtok, Lukasz ; Kleuser, Burkhard ; Edwards, Michael J. ; Gulbins, Erich ; Lentsch, Alex B.
Background & Aims: Exosomes are small membrane vesicles involved in intercellular communication. Hepatocytes are known to release exosomes, but little is known about their biological function. We sought to determine if exosomes derived from hepatocytes contribute to liver repair and regeneration after injury. Methods: Exosomes derived from primary murine hepatocytes were isolated and characterized biochemically and biophysically. Using cultures of primary hepatocytes, we tested whether hepatocyte exosomes induced proliferation of hepatocytes in vitro. Using models of ischemia/reperfusion injury and partial hepatectomy, we evaluated whether hepatocyte exosomes promote hepatocyte proliferation and liver regeneration in vivo. Results: Hepatocyte exosomes, but not exosomes from other liver cell types, induce dose-dependent hepatocyte proliferation in vitro and in vivo. Mechanistically, hepatocyte exosomes directly fuse with target hepatocytes and transfer neutral ceramidase and sphingosine kinase 2 (SK2) causing increased synthesis of sphingosine-1-phosphate (S1P) within target hepatocytes. Ablation of exosomal SK prevents the proliferative effect of exosomes. After ischemia/reperfusion injury, the number of circulating exosomes with proliferative effects increases. Conclusions: Our data shows that hepatocyte-derived exosomes deliver the synthetic machinery to form S1P in target hepatocytes resulting in cell proliferation and liver regeneration after ischemia/reperfusion injury or partial hepatectomy. These findings represent a potentially novel new contributing mechanism of liver regeneration and have important implications for new therapeutic approaches to acute and chronic liver disease. (C) 2015 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
Allyl, dimethylallyl, crotyl, and prenyl ethers of various aromatic ortho-hydroxy carbonyl compounds undergo a tandem sequence of Claisen rearrangement, carbonyl olefination, and cyclization upon microwave irradiation in the presence of a stabilized ylide. The products are multiply substituted 6- or 8-allylated or prenylated coumarins (2H-chromen-2-ones).
Bougeois, Laurie ; de Rafelis, Marc ; Reichart, Gert-Jan ; de Nooijer, Lennart J. ; Dupont-Nivet, Guillaume
Fossil oyster shells are well-suited to provide palaeotemperature proxies from geologic to seasonal timescales due to their ubiquitous occurrence from Triassic to Quaternary sediments, the seasonal nature of their shell growth and their relative strong resistance to post-mortem alteration. However, the common use to translate calcitic oxygen isotopes into palaeotemperatures is challenged by uncertainties in accounting for past seawater delta O-18, especially in shallow coastal environment where oysters calcify. In principle, the Mg/Ca ratio in oyster shells can provide an alternative palaeothermometer. Several studies provided temperature calibrations for this potential proxy based on modem species, nevertheless their application to palaeo-studies remains hitherto unexplored. Here, we show that past temperature variability in seawater can be obtained from Mg/Ca analyses from selected fossil oyster species and specimens. High-resolution Mg/Ca profiles, combined with delta O-18, were obtained along 41 fossil oyster shells of seven different species from the Palaeogene Proto-Paratethys sea (Central Asia) found in similar as well as different depositional age and environments providing comparison. Suitable Mg/Ca profiles, defined by continuous cyclicity and reproducibility within one shell, are found to be consistent for specimens of the same species but differ systematically between species, implying a dominant species-specific effect on the Mg/Ca signal. Two species studied here (Ostrea (Turkostrea) strictiplicata and Sokolowia buhsii) provide an excellent proxy for palaeoclimate reconstruction from China to Europe in Palaeogene marine sediments. More generally, the protocol developed here can be applied to identify other fossil oyster species suitable for palaeoclimate reconstructions. (C) 2015 Elsevier B.V. All rights reserved.
A link between chemical weathering and physical erosion exists at the catchment scale over a wide range of erosion rates(1,2). However, in mountain environments, where erosion rates are highest, weathering may be kinetically limited(3-5) and therefore decoupled from erosion. In active mountain belts, erosion is driven by bedrock landsliding(6) at rates that depend strongly on the occurrence of extreme rainfall or seismicity(7). Although landslides affect only a small proportion of the landscape, bedrock landsliding can promote the collection and slow percolation of surface runoff in highly fragmented rock debris and create favourable conditions for weathering. Here we show from analysis of surface water chemistry in the Southern Alps of New Zealand that weathering in bedrock landslides controls the variability in solute load of these mountain rivers. We find that systematic patterns in surface water chemistry are strongly associated with landslide occurrence at scales from a single hillslope to an entire mountain belt, and that landslides boost weathering rates and river solute loads over decades. We conclude that landslides couple erosion and weathering in fast-eroding uplands and, thus, mountain weathering is a stochastic process that is sensitive to climatic and tectonic controls on mass wasting processes.
Antarctica’s contribution to global sea-level rise has recently been increasing1. Whether its ice discharge will become unstable and decouple from anthropogenic forcing2,3,4 or increase linearly with the warming of the surrounding ocean is of fundamental importance5. Under unabated greenhouse-gas emissions, ocean models indicate an abrupt intrusion of warm circumpolar deep water into the cavity below West Antarctica’s Filchner–Ronne ice shelf within the next two centuries6,7. The ice basin’s retrograde bed slope would allow for an unstable ice-sheet retreat8, but the buttressing of the large ice shelf and the narrow glacier troughs tend to inhibit such instability9,10,11. It is unclear whether future ice loss will be dominated by ice instability or anthropogenic forcing. Here we show in regional and continental-scale ice-sheet simulations, which are capable of resolving unstable grounding-line retreat, that the sea-level response of the Filchner–Ronne ice basin is not dominated by ice instability and follows the strength of the forcing quasi-linearly. We find that the ice loss reduces after each pulse of projected warm water intrusion. The long-term sea-level contribution is approximately proportional to the total shelf-ice melt. Although the local instabilities might dominate the ice loss for weak oceanic warming12, we find that the upper limit of ice discharge from the region is determined by the forcing and not by the marine ice-sheet instability.
The Puna Plateau, adjacent Eastern Cordillera and the Sierras Pampeanas of the central Andes are largely characterized by thick-skinned, basement-involved deformation. The Puna Plateau hosts similar to N-S trending bedrock ranges bounded by deep-seated reverse faults and sedimentary basins. We contribute to the understanding of thick-skinned dynamics in the Puna Plateau by constraining regional kinematics of the poorly understood southern Puna Plateau through a multidisciplinary approach. On the southeastern plateau, sandstone modal composition and detrital zircon U-Pb and apatite fission-track data from Cenozoic strata indicate basin accumulation during the late Eocene to early Oligocene (similar to 38-28 Ma). Provenance analysis reveals the existence of a regional-scale basin covering the southern Puna Plateau during late Eocene to early Oligocene time (similar to 38-28 Ma) that was sourced from both the western plateau and the eastern plateau margin and had a depocenter located to the west. Petrographic and detrital zircon U-Pb data reveal erosion of proximal western and eastern sources after 12 Ma, in mid-late Miocene time. This indicates that the regional basin was compartmentalized into small-scale depocenters by the growth of basement-cored ranges continuing into the late Miocene (similar to 12-8 Ma). We suggest that the Cenozoic history of the southern Puna Plateau records the formation of a regional basin that was possibly driven by lithospheric flexure during the late Eocene to early Oligocene, before the growth of distributed basement-cored ranges starting as early as the late Oligocene. (C) 2015 Elsevier B.V. All rights reserved.
Braune, Steffen ; Gross, M. ; Walter, M. ; Zhou, Shengqiang ; Dietze, Siegfried ; Rutschow, S. ; Lendlein, Andreas ; Tschoepe, C. ; Jung, Friedrich
On the basis of the clinical studies in patients with coronary artery disease (CAD) presenting an increased percentage of activated platelets, we hypothesized that hemocompatibility testing utilizing platelets from healthy individuals may result in an underestimation of the materials' thrombogenicity. Therefore, we investigated the interaction of polymer-based biomaterials with platelets from CAD patients in comparison to platelets from apparently healthy individuals. In vitro static thrombogenicity tests revealed that adherent platelet densities and total platelet covered areas were significantly increased for the low (polydimethylsiloxane, PDMS) and medium (Collagen) thrombogenic surfaces in the CAD group compared to the healthy subjects group. The area per single platelet—indicating the spreading and activation of the platelets—was markedly increased on PDMS treated with PRP from CAD subjects. This could not be observed for collagen or polytetrafluoroethylene (PTFE). For the latter material, platelet adhesion and surface coverage did not differ between the two groups. Irrespective of the substrate, the variability of these parameters was increased for CAD patients compared to healthy subjects. This indicates a higher reactivity of platelets from CAD patients compared to the healthy individuals. Our results revealed, for the first time, that utilizing platelets from apparently healthy donors bears the risk of underestimating the thrombogenicity of polymer-based biomaterials.
Becerril, Laura ; Ubide, Teresa ; Sudo, Masafumi ; Marti, Joan ; Galindo, Ines ; Gale, Carlos ; Maria Morales, Jose ; Yepes, Jorge ; Lago, Marceliano
New age data have been obtained to time constrain the recent Quaternary volcanism of El Hierro (Canary Islands) and to estimate its recurrence rate. We have carried out Ar-40/Ar-39 geochronology on samples spanning the entire volcanostratigraphic sequence of the island and C-14 geochronology on the most recent eruption on the northeast rift of the island: 2280 +/- 30 yr BP. We combine the new absolute data with a revision of published ages onshore, some of which were identified through geomorphological criteria (relative data). We present a revised and updated chronology of volcanism for the last 33 ka that we use to estimate the maximum eruptive recurrence of the island. The number of events per year determined is 9.7 x 10(-4) for the emerged part of the island, which means that, as a minimum, one eruption has occurred approximately every 1000 years. This highlights the need of more geochronological data to better constrain the eruptive recurrence of El Hierro. (C) 2015 Elsevier Ltd. All rights reserved.
Luft, Laura ; Neumann, C. ; Itzerott, S. ; Lausch, A. ; Doktor, D. ; Freude, M. ; Blaum, Niels ; Jeltsch, Florian
The abandonment of military areas leads to succession processes affecting valuable open-land habitats and is considered to be a major threat for European butterflies. We assessed the ability of hyper spectral remote sensing data to spatially predict the occurrence of one of the most endangered butterfly species (Hipparchia statilinus) in Brandenburg (Germany) on the basis of habitat characteristics at a former military training area. Presence-absence data were sampled on a total area of 36 km(2), and N = 65 adult individuals of Hipparchia statilinus could be detected. The floristic composition within the study area was modeled in a three-dimensional ordination space. Occurrence probabilities for the target species were predicted as niches between ordinated floristic gradients by using Regression Kriging of Indicators. Habitat variance could be explained by up to 81 % with spectral variables at a spatial resolution of 2 x 2 m by transferring PLSR models to imagery. Ordinated ecological niche of Hipparchia statilinus was tested against environmental predictor variables. N = 6 variables could be detected to be significantly correlated with habitat preferences of Hipparchia statilinus. They show that Hipparchia statilinus can serve as a valuable indicator for the evaluation of the conservation status of Natura 2000 habitat type 2330 (inland dunes with open Corynephorus and Agrostis grasslands) protected by the Habitat Directive (Council Directive 92/43/EEC). The authors of this approach, conducted in August 2013 at Doberitzer Heide Germany, aim to increase the value of remote sensing as an important tool for questions of biodiversity research and conservation.
Spatio-temporal control of cellular uptake achieved by photoswitchable cell-penetrating peptides
(2016)
The selective uptake of compounds into specific cells of interest is a major objective in cell biology and drug delivery. By incorporation of a novel, thermostable azobenzene moiety we generated peptides that can be switched optically between an inactive state and an active, cell-penetrating state with excellent spatio-temporal control.
Prieske, Olaf ; Mühlbauer, Thomas ; Borde, Ron ; Gube, M. ; Bruhn, S. ; Behm, David George ; Granacher, Urs
Cross-sectional studies revealed that inclusion of unstable elements in core-strengthening exercises produced increases in trunk muscle activity and thus potential extra stimuli to induce more pronounced performance enhancements in youth athletes. Thus, the purpose of the study was to investigate changes in neuromuscular and athletic performance following core strength training performed on unstable (CSTU) compared with stable surfaces (CSTS) in youth soccer players. Thirty-nine male elite soccer players (age: 17 +/- 1 years) were assigned to two groups performing a progressive core strength-training program for 9 weeks (2-3 times/week) in addition to regular in-season soccer training. CSTS group conducted core exercises on stable (i.e., floor, bench) and CSTU group on unstable (e.g., Thera-Band (R) Stability Trainer, Togu (c) Swiss ball) surfaces. Measurements included tests for assessing trunk muscle strength/activation, countermovement jump height, sprint time, agility time, and kicking performance. Statistical analysis revealed significant main effects of test (pre vs post) for trunk extensor strength (5%, P<0.05, d=0.86), 10-20-m sprint time (3%, P<0.05, d=2.56), and kicking performance (1%, P<0.01, d=1.28). No significant Groupxtest interactions were observed for any variable. In conclusion, trunk muscle strength, sprint, and kicking performance improved following CSTU and CSTS when conducted in combination with regular soccer training.
Rakers, Christin ; Schumacher, Fabian ; Meinl, Walter ; Glatt, Hansruedi ; Kleuser, Burkhard ; Wolber, Gerhard
Acting during phase II metabolism, sulfotransferases (SULTs) serve detoxification by transforming a broad spectrum of compounds from pharmaceutical, nutritional, or environmental sources into more easily excretable metabolites. However, SULT activity has also been shown to promote formation of reactive metabolites that may have genotoxic effects. SULT subtype 1E1 (SULT1E1) was identified as a key player in estrogen homeostasis, which is involved in many physiological processes and the pathogenesis of breast and endometrial cancer. The development of an in silico prediction model for SULT1E1 ligands would therefore support the development of metabolically inert drugs and help to assess health risks related to hormonal imbalances. Here, we report on a novel approach to develop a model that enables prediction of substrates and inhibitors of SULT1E1. Molecular dynamics simulations were performed to investigate enzyme flexibility and sample protein conformations. Pharmacophores were developed that served as a cornerstone of the model, and machine learning techniques were applied for prediction refinement. The prediction model was used to screen the DrugBank (a database of experimental and approved drugs): 28% of the predicted hits were reported in literature as ligands of SULT1E1. From the remaining hits, a selection of nine molecules was subjected to biochemical assay validation and experimental results were in accordance with the in silico prediction of SULT1E1 inhibitors and substrates, thus affirming our prediction hypotheses.
Der vorliegende Beitrag gibt einen Überblick über das Thema „Gewalt an Schulen“ und verknüpft es mit gesundheitlichen Aspekten von Schülerinnen und Schülern. Ausgehend von der Annahme, dass Gewalt und Gesundheit zwei Seiten einer Medaille sind, wird der Frage nachgegangen, wie sich Gewalt- und Mobbingphänomene an deutschen Schulen entwickelt haben und welche Zusammenhänge es zur Gesundheit von Schülerinnen und Schülern gibt. Die empirische Basis bilden langjährige Untersuchungen der Autoren, u. a. im Rahmen der internationalen Studie „Health Behaviour in School-aged Children“ (HBSC). Auf der Basis eigener empirischer Studien werden Prävalenzen und Strukturen der Schülergewalt sowie der Zusammenhang von Gewalt und Gesundheit verdeutlicht und Folgerungen für die Gewalt- und Gesundheitsprävention gezogen. Entgegen dramatisierender Einschätzungen in den Medien zeigt sich, dass in den letzten Jahren eine kontinuierliche Verringerung der Mobbing-Problematik an deutschen Schulen zu verzeichnen ist. Mit Blick auf den Zusammenhang von Gewalt und Gesundheit lässt sich ein signifikanter Zusammenhang zwischen dissozialem Verhalten und Parametern der Gesundheit belegen. So weisen Opfer und Täter-Opfer die größten und Unbeteiligte die geringsten gesundheitlichen Probleme auf, während für Täter – entgegen gängiger Annahmen – eher eine positive Grundstimmung kennzeichnend ist. Aufgrund des engen Zusammenhangs von Gewalt und Gesundheit wird die Folgerung abgeleitet, Gewaltprävention- und Gesundheitsförderung mit Schulentwicklung und Schulprogrammarbeit zu verbinden, um nachhaltige Präventionswirkung zu erzielen.
The article gives an overview of violence at school combined with the health of students. Based on the assumption that violence and health are two sides of the same coin, the article considers the question of the development of violence and bullying phenomena at German schools and its relation to student health. Long-term studies by the authors, such as the international Health Behavior in School-aged Children (HBSC) study, constitute the empirical basis. Prevalence and structures of student violence, as well as the relation between violence and health, are clarified and consequences for the prevention of violence and health are shown on the basis of our own empirical studies. Contrary to dramatic estimates in the media, a continuous reduction of bullying problems at German schools has been registered in recent years. In reference to the relation between violence and health, a significant correlation between dissocial behavior and health parameters was found. Victims and bully-victims show the most health problems and innocent bystanders the least health problems. In contrast to previous findings, bullies show a more positive prevailing mood. Due to the found associations between violence and health, it appears reasonable to combine violence prevention and health promotion measures to achieve sustainable prevention effects.
Brietzke, Thomas Martin ; Kelling, Alexandra ; Schilde, Uwe ; Mickler, Wulfhard ; Holdt, Hans-Jürgen
The first heterodinuclear ruthenium(II) complexes of the 1,6,7,12-tetraazaperylene (tape) bridging ligand with iron(II), cobalt(II), and nickel(II) were synthesized and characterized. The metal coordination sphere in this complexes is filled by the tetradentate N,N-dimethyl-2,11-diaza[3.3](2,6)-pyridinophane (L-N4Me2) ligand, yielding complexes of the general formula [(L-N4Me2)Ru(mu-tape)M(L-N4Me2)](ClO4)(2)(PF6)(2) with M = Fe {[2](ClO4)(2)(PF6)(2)}, Co {[3](ClO4)(2)(PF6)(2)}, and Ni {[4](ClO4)(2)(PF6)(2)}. Furthermore, the heterodinuclear tape ruthenium(II) complexes with palladium(II)- and platinum(II)-dichloride [(bpy)(2)Ru(-tape)PdCl2](PF6)(2) {[5](PF6)(2)} and [(dmbpy)(2)Ru(-tape)PtCl2](PF6)(2) {[6](PF6)(2)}, respectively were also prepared. The molecular structures of the complex cations [2](4+) and [4](4+) were discussed on the basis of the X-ray structures of [2](ClO4)(4)MeCN and [4](ClO4)(4)MeCN. The electrochemical behavior and the UV/Vis absorption spectra of the heterodinuclear tape ruthenium(II) complexes were explored and compared with the data of the analogous mono- and homodinuclear ruthenium(II) complexes of the tape bridging ligand.
Complementary to the well-established zwitterionic monomer 3-((3-methacrylamidopropyl) dimethylammonio) propane-1-sulfonate (SPP), the closely related monomers 2-hydroxy-3-((3-methacrylamidopropyl) dimethylammonio) propane-1-sulfonate (SHPP) and 4-((3-methacrylamidopropyl) dimethylammonio)butane- 1-sulfonate (SBP) were synthesised and polymerised by reversible addition-fragmentation chain transfer (RAFT) polymerisation, using a fluorophore labeled RAFT agent. The polyzwitterions of systematically varied molar masses were characterised with respect to their solubility in water and aqueous salt solutions. Both poly(sulfobetaine)s show thermoresponsive behaviour in water, exhibiting phase separation at low temperatures and upper critical solution temperatures (UCST). For both polySHPP and polySBP, cloud points depend notably on the molar mass, and are much higher in D2O than in H2O. Also, the cloud points are effectively modulated by the addition of salts. The individual effects can be in parts correlated to the Hofmeister series for the anions studied. Still, they depend in a complex way on the concentration and the nature of the added electrolytes, on the one hand, and on the detailed nature of the spacer group separating the anionic and the cationic charges of the betaine moiety, on the other hand. As anticipated, the cloud points of polySBP are much higher than the ones of the analogous polySPP of identical molar mass. Surprisingly, the cloud points of polySHPP are also somewhat higher than the ones of their polySPP analogues, despite the additional hydrophilic hydroxyl group present in the spacer separating the ammonium and the sulfonate moieties. These findings point to a complicated interplay of the various hydrophilic components in polyzwitterions with respect to their overall hydrophilicity. Thus, the spacer group in the betaine moiety proves to be an effective additional molecular design parameter, apparently small variations of which strongly influence the phase behaviour of the polyzwitterions in specific aqueous environments.
A unique fabrication process of low molar mass, crystalline polypeptoid fibers is described. Thermoresponsive fiber mats are prepared by electrospinning a homogeneous blend of semicrystalline poly(N-(n-propyl) glycine) (PPGly; 4.1 kDa) with high molar mass poly(ethylene oxide) (PEO). Annealing of these fibers at approximate to 100 degrees C selectively removes the PEO and produces stable crystalline fiber mats of pure PPGly, which are insoluble in aqueous solution but can be redissolved in methanol or ethanol. The formation of water-stable polypeptoid fiber mats is an important step toward their utilization in biomedical applications such as tissue engineering or wound dressing.
Warming and eutrophication are two of the most important global change stressors for natural ecosystems, but their interaction is poorly understood. We used a dynamic model of complex, size-structured food webs to assess interactive effects on diversity and network structure. We found antagonistic impacts: Warming increases diversity in eutrophic systems and decreases it in oligotrophic systems. These effects interact with the community size structure: Communities of similarly sized species such as parasitoid-host systems are stabilized by warming and destabilized by eutrophication, whereas the diversity of size-structured predator-prey networks decreases strongly with warming, but decreases only weakly with eutrophication. Nonrandom extinction risks for generalists and specialists lead to higher connectance in networks without size structure and lower connectance in size-structured communities. Overall, our results unravel interactive impacts of warming and eutrophication and suggest that size structure may serve as an important proxy for predicting the community sensitivity to these global change stressors.
Skipping a grade, one specific form of acceleration, is an intervention used for gifted students. Quantitative research has shown acceleration to be a highly successful intervention regarding academic achievement, but less is known about the social–emotional outcomes of grade-skipping. In the present study, the authors used the grounded theory approach to examine the experiences of seven gifted students aged 8 to 16 years who skipped a grade. The interviewees perceived their “feeling of being in the wrong place” before the grade-skipping as strongly influenced by their teachers, who generally did not respond adequately to their needs. We observed a close interrelationship between the gifted students’ intellectual fit and their social situation in class. Findings showed that the grade-skipping in most of the cases bettered the situation in school intellectually as well as socially, but soon further interventions, for instance, a specialized and demanding class- or subject-specific acceleration were added to provide sufficiently challenging learning opportunities.
1. Plant-plant interactions may critically modify the impact of climate change on plant communities. However, the magnitude and even direction of potential future interactions remains highly debated, especially for water-limited ecosystems. Predictions range from increasing facilitation to increasing competition with future aridification. 2. The different methodologies used for assessing plant-plant interactions under changing environmental conditions may affect the outcome but they are not equally represented in the literature. Mechanistic experimental manipulations are rare compared with correlative approaches that infer future patterns from current observations along spatial climatic gradients. 3. Here, we utilize a unique climatic gradient in combination with a large-scale, long-term experiment to test whether predictions about plant-plant interactions yield similar results when using experimental manipulations, spatial gradients or temporal variation. We assessed shrub-annual interactions in three different sites along a natural rainfall gradient (spatial) during 9 years of varying rainfall (temporal) and 8 years of dry and wet manipulations of ambient rainfall (experimental) that closely mimicked regional climate scenarios. 4. The results were fundamentally different among all three approaches. Experimental water manipulations hardly altered shrub effects on annual plant communities for the assessed fitness parameters biomass and survival. Along the spatial gradient, shrub effects shifted from clearly negative to mildly facilitative towards drier sites, whereas temporal variation showed the opposite trend: more negative shrub effects in drier years. 5. Based on our experimental approach, we conclude that shrub-annual interaction will remain similar under climate change. In contrast, the commonly applied space-for-time approach based on spatial gradients would have suggested increasing facilitative effects with climate change. We discuss potential mechanisms governing the differences among the three approaches. 6. Our study highlights the critical importance of long-term experimental manipulations for evaluating climate change impacts. Correlative approaches, for example along spatial or temporal gradients, may be misleading and overestimate the response of plant-plant interactions to climate change.
In nature, plants often encounter chronic or recurring stressful conditions. Recent results indicate that plants can remember a past exposure to stress to be better prepared for a future stress incident. However, the molecular basis of this is poorly understood. Here, we report the involvement of chromatin modifications in the maintenance of acquired thermotolerance (heat stress [HS] memory). HS memory is associated with the accumulation of histone H3 lysine 4 di- and trimethylation at memory-related loci. This accumulation outlasts their transcriptional activity and marks them as recently transcriptionally active. High accumulation of H3K4 methylation is associated with hyper-induction of gene expression upon a recurring HS. This transcriptional memory and the sustained accumulation of H3K4 methylation depend on HSFA2, a transcription factor that is required for HS memory, but not initial heat responses. Interestingly, HSFA2 associates with memory-related loci transiently during the early stages following HS. In summary, we show that transcriptional memory after HS is associated with sustained H3K4 hyper-methylation and depends on a hit-and-run transcription factor, thus providing a molecular framework for HS memory.
The competitive extraction of Cr(III) onto Nauclea diderrichii seed epicarp doped with MnO2 nanoparticles (MnO2 nano-bioextractant (MNB)) in a single and binary batch system was studied. For validity of experimental data, chi square test, root mean square error, sum of the square errors, hybrid fractional error function, Marquart’s percent standard deviation and standard absolute error were used. Among the kinetic models used, pseudo-second-order and Langmuir equations gave the best fits for the experimental data, with qe (mg g) for the uptake of Cr(III) in single metal system onto MNB, then Cr(III) with Cd(II), Pb(II), Hg(II), KCl and CaCl2 in binary metal systems onto MNB were 2.611, then 1.989, 1.016, 2.208, 1.249 and 1.868 from kinetic standpoint, respectively. The initial sorption rates, h (mg/g/min), and half lives, t1/2 (min), for the uptake of Cr(III) in single metal system onto MNB, then Cr(III) with Cd(II), Pb(II), Hg(II), KCl and CaCl2 in binary metal system onto MNB were 3.497, then 2.311, 2.274, 0.242, 2.956, 45.568 and 0.747, then 5.769, 1.766, 12.144, 1.762, and 2.415, respectively. Physicochemical surface analyses such as pH of point of zero charge, Brunauer–Emmett–Teller single point and multi-point techniques for surface area analyses, scanning electron microscopy and transmission electron microscopy were done on MNB and MnO2 nanoparticles in order to understand their surface microstructures. Desorption study showed that MNB can be recycled and used for future study. Hence, MNB showed good potential to remediate Cr(III) from wastewaters and polluted water.
Mediated bioelectrochemical system for biosensing the cell viability of Staphylococcus aureus
(2016)
Staphylococcus aureus is one of the most dangerous human pathogens and is the cause of numerous illnesses ranging from moderate skin infections to life-threatening diseases. Despite advances made in identifying microorganisms, rapid detection methods for the viability of bacteria are still missing. Here, we report a rapid electrochemical assay for cell viability combining the use of double redox mediators and multiwall carbon nanotubes-screen printed electrodes (MWCNTs-SPE), ferricyanide (FCN) and 2,6-dichlorophenolindophenol (DCIP), which served as electron shuttle to enable the bacterial-electrode communications. The current originating from the metabolically active cells was recorded for probing the activity of the intracellular redox centers. Blocking of the respiratory chain pathways with electron transfer inhibitors demonstrated the involvement of the electron transport chain in the reaction. A good correlation between the number of the metabolically active cells and the current was obtained. The proposed assay has been exploited for monitoring cell proliferation of S. aureus during the growth. The sensitivity of the detection method reached 0.1 OD600. Therefore, the technique described is promising for estimating the cell number, measuring the cell viability, and probing intracellular redox center(s).
Previous work on spatial-numerical association (SNAs) included either spatially distributed stimuli or responses. This raises the possibility that the inferred spatial nature of number concepts was a methodological artifact. We present results from a novel task that involves two categories (spatially oriented objects and number magnitudes) and dissociates spatial classification from number classification. The results reveal SNAs without inferential limitations of previous work and point to a working memory mechanism that transfers spatial coding across categories.
Cetinkaplan, Mete ; Pourteau, Amaury ; Candan, Osman ; Koralay, O. Ersin ; Oberhänsli, Roland ; Okay, Aral I. ; Chen, Fukun ; Kozlu, Huseyin ; Sengun, Firat
The Alanya Massif, which is located to the south of central Taurides in Turkey, presents a typical nappe pile consisting of thrust sheets with contrasting metamorphic histories. In two thrust sheets, Sugozu and GundogmuAY nappes, HP metamorphism under eclogite (550-567 A degrees C/14-18 kbar) and blueschist facies (435-480 A degrees C/11-13 kbar) conditions have been recognized, respectively. Whereas the rest of the Massif underwent MP metamorphism under greenschist to amphibolite facies (525-555 A degrees C/6.5-7.5 kbar) conditions. Eclogite facies metamorphism in Sugozu nappe, which consists of homogeneous garnet-glaucophane-phengite schists with eclogite lenses is dated at 84.8 +/- A 0.8, 84.7 +/- A 1.5 and 82 +/- A 3 Ma (Santonian-Campanian) by Ar-40/Ar-39 phengite, U/Pb zircon and rutile dating methods, respectively. Similarly, phengites in GundogmuAY nappe representing an accretionary complex yield 82-80 Ma (Campanian) ages for blueschist facies metamorphism. During the exhumation, the retrograde overprint of the HP units under greenschist-amphibolite facies conditions and tectonic juxtaposition with the Barrovian units occurred during Campanian (75-78 Ma). Petrological and geochronological data clearly indicate a similar Late Cretaceous tectonometamorphic evolution for both Alanya (84-75 Ma) and Bitlis (84-72 Ma) Massifs. They form part of a single continental sliver (Alanya-Bitlis microcontinent), which was rifted from the southern part of the Anatolide-Tauride platform. The P-T-t coherence between two Massifs suggests that both Massifs have been derived from the closure of the same ocean (Alanya-Bitlis Ocean) located to the south of the Anatolide-Tauride block by a northward subduction. The boundary separating the autochthonous Tauride platform to the north from both the Alanya and Bitlis Massifs to the south represents a suture zone, the Pamphylian-Alanya-Bitlis suture.
Anatolia’s high-pressure metamorphic belts are characterized in part by a Neotethyan stratigraphic succession that includes a mid-Cretaceous hemi-pelagic marble sequence. This unit contains, towards its stratigraphic top, dm-to-m-long radiating calcitic rods forming rosette-like textures. Here, we refer to these features as “Rosetta Marble”. The remarkable textural similarity of non-metamorphic selenite crystals and radiating calcite rods in the Rosetta Marble strongly suggests that these textures represent pseudomorphs after selenites. Metamorphosed hemi-pelagic limestones, dominated by Rosetta selenite pseudomorphs, are alternating with siliceous meta-sediments containing relictic radiolaria tests. This stratigraphic pattern is indicative of transient phases characterized by evaporites precipitated from basinal brines alternating with non-evaporative hemi-pelagic deposition from normal-marine seawater. The regional distribution of Rosetta Marble exposures over 600 km is indicative of basin-scale evaporitic intervals. High-pressure, low-temperature metamorphism of these rocks is witnessed by Sr-rich (up to 3500 ppm), fibrous calcite pseudomorphs after aragonite and isolated aragonite inclusions in quartz. Peak metamorphic conditions of 1.2 GPa and 300–350 °C are attested by high-Si white mica thermobarometry. The Rosetta Marble case example examines the potential to unravel the complete history from deposition to diagenesis and metamorphism of meta-sedimentary rocks.
This paper addresses the lithosphere-scale subduction-collision history of the eastern termination of the Aegean retreating subduction system, i.e. western Anatolia. Although there is some general consensus on the protracted subduction evolution of the Aegean since the early Cenozoic at least, correlation with western Anatolia has been widely debated for more than several decades. In western Anatolia, three main tectonic configurations have been envisaged in the past years to reconstruct slab dynamics during the closure of the Neotethyan oceanic realm since the Late Cretaceous. Some authors have suggested an Aegean-type scenario, with the continuous subduction of a single lithospheric slab, punctuated by episodic slab roll-back and trench retreat, whereas others assumed a discontinuous subduction history marked by intermittent slab break-off during either the Campanian (ca. 75 Ma) or the Early Eocene (ca. 55-50 Ma). The third view implies three partly contemporaneous subduction zones. Our review of these models points to key debated aspects that can be re-evaluated in the light of multidisciplinary constraints from the literature. Our discussion leads us to address the timing of subduction initiation, the existence of hypothetical ocean basins, the number of intervening subduction zones between the Taurides and the Pontides, the palaeogeographic origin of tectonic units and the possibility for slab break-off during either the Campanian or the Early Eocene. Thence, we put forward a favoured tectonic scenario featuring two successive phases of subduction of a single lithospheric slab and episodic accretion of two continental domains separated by a continental trough, representing the eastern end of the Cycladic Ocean of the Aegean. The lack of univocal evidence for slab break-off in western Anatolia and southward-younging HP/LT metamorphism in continental tectonic units (from similar to 85, 70 to 50 Ma) in the Late Cretaceous-Palaeogene period suggests continuous subduction since similar to 110 Ma, marked by roll-back episodes in the Palaeocene and the Oligo-Miocene, and slab tearing below western Anatolia during the Miocene.
Passarelli, Luigi ; Hainzl, Sebastian ; Cesca, Simone ; Maccaferri, Francesco ; Mucciarelli, Marco ; Roessler, Dirk ; Corbi, Fabio ; Dahm, Torsten ; Rivalta, Eleonora
A unique assemblage including kumdykolite and kokchetavite, polymorphs of albite and K-feldspar, respectively, together with cristobalite, micas, and calcite has been identified in high-pressure granulites of the Orlica-Snieznik dome (Bohemian Massif) as the product of partial melt crystallization in preserved nanogranites. Previous reports of both kumdykolite and kokchetavite in natural rocks are mainly from samples that passed through the diamond stability field. However, because the maximum pressure recorded in these host rocks is <3 GPa, our observations indicate that high pressure is not required for the formation of kumdykolite and kokchetavite, and their presence is not therefore an indicator of ultrahigh-pressure conditions. Detailed microstructural and microchemical investigation of these inclusions indicates that such phases should instead be regarded as (1) a direct mineralogical criteria to identify former melt inclusions with preserved original compositions, including H2O and CO2 contents and (2) indicators of rapid cooling of the host rocks. Thus, the present study provides novel criteria for the interpretation of melt inclusions in natural rocks and allows a more rigorous characterization of partial melts during deep subduction to mantle depth as well as their behavior on exhumation.
The ground-state classical path approximation is utilized to compute molecular absorption spectra in a mixed quantum-classical frame. To improve the description for high-frequency vibrational satellites, related quantum correction factors are introduced. The improved method is demonstrated for the Q(y),and Q(x)-bands of pheophorbide a. (C) 2015 Elsevier B.V. All rights reserved.
Catalytic amounts of a weak base are sufficient to induce the decomposition of anthracene endoperoxides to anthraquinone. The mechanism has been elucidated by isolation of intermediates in combination with DFT calculations. The whole process is suitable for the convenient generation of hydrogen peroxide under very mild conditions.
Pavlova, Viola ; Grimm, Volker ; Dietz, Rune ; Sonne, Christian ; Vorkamp, Katrin ; Riget, Frank F. ; Letcher, Robert J. ; Gustavson, Kim ; Desforges, Jean-Pierre ; Nabe-Nielsen, Jacob
Polychlorinated biphenyls (PCBs) can cause endocrine disruption, cancer, immunosuppression, or reproductive failure in animals. We used an individual-based model to explore whether and how PCB-associated reproductive failure could affect the dynamics of a hypothetical polar bear (Ursus maritimus) population exposed to PCBs to the same degree as the East Greenland subpopulation. Dose-response data from experimental studies on a surrogate species, the mink (Mustela vision), were used in the absence of similar data for polar bears. Two alternative types of reproductive failure in relation to maternal sum-PCB concentrations were considered: increased abortion rate and increased cub mortality. We found that the quantitative impact of PCB-induced reproductive failure on population growth rate depended largely on the actual type of reproductive failure involved. Critical potencies of the dose-response relationship for decreasing the population growth rate were established for both modeled types of reproductive failure. Comparing the model predictions of the age-dependent trend of sum-PCBs concentrations in females with actual field measurements from East Greenland indicated that it was unlikely that PCB exposure caused a high incidence of abortions in the subpopulation. However, on the basis of this analysis, it could not be excluded that PCB exposure contributes to higher cub mortality. Our results highlight the necessity for further research on the possible influence of PCBs on polar bear reproduction regarding their physiological pathway. This includes determining the exact cause of reproductive failure, i.e., in utero exposure versus lactational exposure of offspring; the timing of offspring death; and establishing the most relevant reference metrics for the dose-response relationship.
Objectives: To reanalyze the between-population variance in height, weight, and body mass index (BMI), and to provide a globally applicable technique for generating synthetic growth reference charts. Methods: Using a baseline set of 196 female and 197 male growth studies published since 1831, common factors of height, weight, and BMI are extracted via Principal Components separately for height, weight, and BMI. Combining information from single growth studies and the common factors using in principle a Bayesian rationale allows for provision of completed reference charts. Results: The suggested approach can be used for generating synthetic growth reference charts with LMS values for height, weight, and BMI, from birth to maturity, from any limited set of height and weight measurements of a given population. Conclusion: Generating synthetic growth reference charts by incorporating information from a large set of reference growth studies seems suitable for populations with no autochthonous references at hand yet. (C) 2015 Wiley Periodicals, Inc.
Natural vision is characterized by alternating sequences of rapid gaze shifts (saccades) and fixations. During fixations, microsaccades and slower drift movements occur spontaneously, so that the eye is never motionless. Theoretical models of fixational eye movements predict that microsaccades are dynamically coupled to slower drift movements generated immediately before microsaccades, which might be used as a criterion to distinguish microsaccades from small voluntary saccades. Here we investigate a sequential scanning task, where participants generate goal-directed saccades and microsaccades with overlapping amplitude distributions. We show that properties of microsaccades are correlated with precursory drift motion, while amplitudes of goal-directed saccades do not dependent on previous drift epochs. We develop and test a mathematical model that integrates goal-directed and fixational eye movements, including microsaccades. Using model simulations, we reproduce the experimental finding of correlations within fixational eye movement components (i.e., between physiological drift and microsaccades) but not between goal-directed saccades and fixational drift motion. These results lend support to a functional difference between microsaccades and goal-directed saccades, while, at the same time, both types of behavior may be part of an oculomotor continuum that is quantitatively described by our mathematical model. (C) 2015 Elsevier Ltd. All rights reserved.
Park, H. ; Walta, S. ; Rosencrantz, Ruben R. ; Koerner, A. ; Schulte, Christoph ; Elling, L. ; Richtering, Walter ; Böker, Alexander
We introduce a novel double-hydrophilic hydroxyethylmethacrylate (HEMA) based diblock glycopolymer which self-assembles into homogeneous spherical micellar structures in water. The micellar structure renders surface-oriented N-acetylglucocosamine (GlcNAc) sugar moieties for strong multivalent glycan-mediated lectin binding. Structural analysis and lectin binding is performed by microscopy methods, dynamic light scattering (DLS) and two-focus fluorescence correlation spectroscopy (2fFCS), revealing a novel micellar type of multivalent sugar binding scaffold with high potential for biomedical applications.
Paijmans, Johanna L. A. ; Fickel, Jörns ; Courtiol, Alexandre ; Hofreiter, Michael ; Foerster, Daniel W.
Abstract
By combining high-throughput sequencing with target enrichment (‘hybridization capture’), researchers are able to obtain molecular data from genomic regions of interest for projects that are otherwise constrained by sample quality (e.g. degraded and contamination-rich samples) or a lack of a priori sequence information (e.g. studies on nonmodel species). Despite the use of hybridization capture in various fields of research for many years, the impact of enrichment conditions on capture success is not yet thoroughly understood. We evaluated the impact of a key parameter – hybridization temperature – on the capture success of mitochondrial genomes across the carnivoran family Felidae. Capture was carried out for a range of sample types (fresh, archival, ancient) with varying levels of sequence divergence between bait and target (i.e. across a range of species) using pools of individually indexed libraries on Agilent SureSelect™ arrays. Our results suggest that hybridization capture protocols require specific optimization for the sample type that is being investigated. Hybridization temperature affected the proportion of on-target sequences following capture: for degraded samples, we obtained the best results with a hybridization temperature of 65 °C, while a touchdown approach (65 °C down to 50 °C) yielded the best results for fresh samples. Evaluation of capture performance at a regional scale (sliding window approach) revealed no significant improvement in the recovery of DNA fragments with high sequence divergence from the bait at any of the tested hybridization temperatures, suggesting that hybridization temperature may not be the critical parameter for the enrichment of divergent fragments.
Das Anliegen der vorliegenden Studie ist es, zu prüfen, inwiefern eine differenzierende Unterrichtspraxis in den Fächern Mathematik und Deutsch im Zusammenhang mit der Wahrnehmung von Leistungsheterogenität und der Diagnosegenauigkeit von Lehrkräften steht. Der Untersuchung lagen Leistungsdaten aus den Vergleichsarbeiten (VERA-8) und Lehrerurteile über N = 1803 Schüler/innen der achten Jahrgangsstufe an Gymnasien im Land Brandenburg zugrunde sowie Schülereinschätzungen zur Differenzierung im Unterricht. Hierarchische Regressionsanalysen zeigen, dass Mathematiklehrkräfte aus Sicht der Schüler/innen umso stärker differenzierend unterrichten, je besser sie die Leistungsstände der Schüler/innen einschätzen können. Im Fach Deutsch findet differenzierender Unterricht in geringerem Umfang statt und es zeigen sich keine Zusammenhänge mit der Diagnosegenauigkeit.
The aim of the present study is to examine in how far a differentiating teaching practice in Math and German lessons is linked with the perception of heterogeneity in student performance and with the teachers´ diagnostic accuracy. The study is based on performance data from comparative tests (VERA-8) and teacher assessments of N = 1803 students attending 8th grade at grammar schools in the federal state of Brandenburg and on student assessments on differentiation in teaching. Hierarchical regression analyses show that, from the students´ perspective, Math teachers differentiate the more in their teaching the better they are able to assess the students´ performance level. In German lessons, differentiating forms of teaching are less often applied and there are no links with diagnostic accuracy.
Lehmeyer, Mona ; Kanofsky, Konstantin ; Hanko, Erik K. R. ; Ahrendt, Sarah ; Wehrs, Maren ; Machens, Fabian ; Hehl, Reinhard
Synthetic promoters are important for temporal and spatial gene expression in transgenic plants. To identify novel microbe-associated molecular pattern (MAMP)-responsive cis-regulatory sequences for synthetic promoter design, a combination of bioinformatics and experimental approaches was employed. One cis-sequence was identified which confers strong MAMP-responsive reporter gene activity with low background activity. The 35-bp-long cis-sequence was identified in the promoter of the Arabidopsis thaliana DJ1E gene, a homologue of the human oncogene DJ1. In this study, this cis-sequence is shown to be a tripartite cis-regulatory module (CRM). A synthetic promoter with four copies of the CRM linked to a minimal promoter increases MAMP-responsive reporter gene expression compared to the wild-type DJ1E promoter. The CRM consists of two WT-boxes (GGACTTTT and GGACTTTG) and a variant of the GCC-box (GCCACC), all required for MAMP and salicylic acid (SA) responsivity. Yeast one-hybrid screenings using a transcription factor (TF)-only prey library identified two AP2/ERFs, ORA59 and ERF10, interacting antagonistically with the CRM. ORA59 activates reporter gene activity and requires the consensus core sequence GCCNCC for gene expression activation. ERF10 down-regulates MAMP-responsive gene expression. No TFs interacting with the WT-boxes GGACTTTT and GGACTTTG were selected in yeast onehybrid screenings with the TF-only prey library. In transgenic Arabidopsis, the synthetic promoter confers strong and specific reporter gene activity in response to biotrophs and necrotrophs as well as SA.
In this paper two new methods for the design of fault-tolerant pipelined sequential and combinational circuits, called Error Detection and Partial Error Correction (EDPEC) and Full Error Detection and Correction (FEDC), are described. The proposed methods are based on an Error Detection Logic (EDC) in the combinational circuit part combined with fault tolerant memory elements implemented using fault tolerant master-slave flip-flops. If a transient error, due to a transient fault in the combinational circuit part is detected by the EDC, the error signal controls the latching stage of the flip-flops such that the previous correct state of the register stage is retained until the transient error disappears. The system can continue to work in its previous correct state and no additional recovery procedure (with typically reduced clock frequency) is necessary. The target applications are dataflow processing blocks, for which software-based recovery methods cannot be easily applied. The presented architectures address both single events as well as timing faults of arbitrarily long duration. An example of this architecture is developed and described, based on the carry look-ahead adder. The timing conditions are carefully investigated and simulated up to the layout level. The enhancement of the baseline architecture is demonstrated with respect to the achieved fault tolerance for the single event and timing faults. It is observed that the number of uncorrected single events is reduced by the EDPEC architecture by 2.36 times compared with previous solution. The FEDC architecture further reduces the number of uncorrected events to zero and outperforms the Triple Modular Redundancy (TMR) with respect to correction of timing faults. The power overhead of both new architectures is about 26-28% lower than the TMR. (C) 2015 Elsevier Ltd. All rights reserved.
Individuals with agrammatic Broca's aphasia experience difficulty when processing reversible non-canonical sentences. Different accounts have been proposed to explain this phenomenon. The Trace Deletion account (Grodzinsky, 1995, 2000, 2006) attributes this deficit to an impairment in syntactic representations, whereas others (e.g., Caplan, Waters, Dede, Michaud, & Reddy, 2007; Haarmann, Just, & Carpenter, 1997) propose that the underlying structural representations are unimpaired, but sentence comprehension is affected by processing deficits, such as slow lexical activation, reduction in memory resources, slowed processing and/or intermittent deficiency, among others. We test the claims of two processing accounts, slowed processing and intermittent deficiency, and two versions of the Trace Deletion Hypothesis (TDH), in a computational framework for sentence processing (Lewis & Vasishth, 2005) implemented in ACT-R (Anderson, Byrne, Douglass, Lebiere, & Qin, 2004). The assumption of slowed processing is operationalized as slow procedural memory, so that each processing action is performed slower than normal, and intermittent deficiency as extra noise in the procedural memory, so that the parsing steps are more noisy than normal. We operationalize the TDH as an absence of trace information in the parse tree. To test the predictions of the models implementing these theories, we use the data from a German sentence—picture matching study reported in Hanne, Sekerina, Vasishth, Burchert, and De Bleser (2011). The data consist of offline (sentence-picture matching accuracies and response times) and online (eye fixation proportions) measures. From among the models considered, the model assuming that both slowed processing and intermittent deficiency are present emerges as the best model of sentence processing difficulty in aphasia. The modeling of individual differences suggests that, if we assume that patients have both slowed processing and intermittent deficiency, they have them in differing degrees.
Arf-like proteins (ARLs) are ubiquitously expressed small G proteins of the RAS superfamily. In photoreceptors, ARL2 and ARL3 participate in the trafficking of lipidated membrane-associated proteins and colocalize in the inner segment with UNC119A and PDE delta. UNC119A and PDE delta are acyl-and prenyl-binding proteins, respectively, involved in trafficking of acylated (transducin-alpha subunit, nephrocystin NPHP3) and prenylated proteins (GRK1, PDE6). Germline Arl3 knockout mice do not survive beyond postnatal day 21 and display ciliary defects in multiple organs (kidney, liver and pancreas) as well as retinal degeneration. Conditional knockouts will be necessary to delineate mechanisms of protein transport in retina disease.
Muino, Jose M. ; de Bruijn, Suzanne ; Pajoro, Alice ; Geuten, Koen ; Vingron, Martin ; Angenent, Gerco C. ; Kaufmann, Kerstin
Flower development is controlled by the action of key regulatory transcription factors of the MADS-domain family. The function of these factors appears to be highly conserved among species based on mutant phenotypes. However, the conservation of their downstream processes is much less well understood, mostly because the evolutionary turnover and variation of their DNA-binding sites (BSs) among plant species have not yet been experimentally determined. Here, we performed comparative ChIP (chromatin immunoprecipitation)-seq experiments of the MADS-domain transcription factor SEPALLATA3 (SEP3) in two closely related Arabidopsis species: Arabidopsis thaliana and A. lyrata which have very similar floral organ morphology. We found that BS conservation is associated with DNA sequence conservation, the presence of the CArG-box BS motif and on the relative position of the BS to its potential target gene. Differences in genome size and structure can explain that SEP3 BSs in A. lyrata can be located more distantly to their potential target genes than their counterparts in A. thaliana. In A. lyrata, we identified transposition as a mechanism to generate novel SEP3 binding locations in the genome. Comparative gene expression analysis shows that the loss/gain of BSs is associated with a change in gene expression. In summary, this study investigates the evolutionary dynamics of DNA BSs of a floral key-regulatory transcription factor and explores factors affecting this phenomenon.