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The use of chironomids (Diptera: Chironomidae) as indicators of changes in natural and climatic settings allows one to reconstruct paleoclimatic and paleoenvironmental conditions. Based on the results of lithological and chironomid analyses, the loss on ignition (LOI) values, as well as the results of radiocarbon dating (C-14 AMS), new data on the paleoclimate in the Karelian Isthmus, in particular the quantitative reconstruction of the mean July temperature (T (July), A degrees C) in the Late Pleistocene and Holocene and the evolution of the ecosystem of Medvedevskoe Lake have been obtained.