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Background
Epidemiological data indicate elevated psychosocial health risks for physicians, e.g., burnout, depression, marital disturbances, alcohol and substance abuse, and suicide. The purpose of this study was to identify psychosocial health resources and risk factors in profession-related behaviour and experience patterns of medical students and physicians that may serve as a basis for appropriate health promoting interventions.
Methods
The questionnaire -Related Behaviour and Experience "Work administered in cross-sectional surveys to students in the first (n = 475) and in the fifth year of studies (n = 355) in required courses at three German universities and to physicians in early professional life in the vicinity of these universities (n = 381).
Results
Scores reflecting a healthy behaviour pattern were less likely in physicians (16.7%) compared to 5th year (26.0%) and 1st year students (35.1%) while scores representing unambitious and resigned patterns were more common among physicians (43.4% vs. 24.4% vs. 41.0% and 27.3% vs. 17.2% vs. 23.3 respectively). Female and male responders differed in the domains professional commitment, resistance to stress and emotional well-being. Female physicians on average scored higher in the dimensions resignation tendencies, satisfaction with life and experience of social support, and lower in career ambition.
Conclusion
The results show distinct psychosocial stress patterns among medical students and physicians. Health promotion and prevention of psychosocial symptoms and impairments should be integrated as a required part of the medical curriculum and be considered an important issue during the further training of physicians.
QuantPrime
(2008)
Background
Medium- to large-scale expression profiling using quantitative polymerase chain reaction (qPCR) assays are becoming increasingly important in genomics research. A major bottleneck in experiment preparation is the design of specific primer pairs, where researchers have to make several informed choices, often outside their area of expertise. Using currently available primer design tools, several interactive decisions have to be made, resulting in lengthy design processes with varying qualities of the assays.
Results
Here we present QuantPrime, an intuitive and user-friendly, fully automated tool for primer pair design in small- to large-scale qPCR analyses. QuantPrime can be used online through the internet http://www.quantprime.de/ or on a local computer after download; it offers design and specificity checking with highly customizable parameters and is ready to use with many publicly available transcriptomes of important higher eukaryotic model organisms and plant crops (currently 295 species in total), while benefiting from exon-intron border and alternative splice variant information in available genome annotations. Experimental results with the model plant Arabidopsis thaliana, the crop Hordeum vulgare and the model green alga Chlamydomonas reinhardtii show success rates of designed primer pairs exceeding 96%.
Conclusion
QuantPrime constitutes a flexible, fully automated web application for reliable primer design for use in larger qPCR experiments, as proven by experimental data. The flexible framework is also open for simple use in other quantification applications, such as hydrolyzation probe design for qPCR and oligonucleotide probe design for quantitative in situ hybridization. Future suggestions made by users can be easily implemented, thus allowing QuantPrime to be developed into a broad-range platform for the design of RNA expression assays.
Background: Haplotype inference based on unphased SNP markers is an important task in population genetics. Although there are different approaches to the inference of haplotypes in diploid species, the existing software is not suitable for inferring haplotypes from unphased SNP data in polyploid species, such as the cultivated potato (Solanum tuberosum). Potato species are tetraploid and highly heterozygous.
Results: Here we present the software SATlotyper which is able to handle polyploid and polyallelic data. SATlo-typer uses the Boolean satisfiability problem to formulate Haplotype Inference by Pure Parsimony. The software excludes existing haplotype inferences, thus allowing for calculation of alternative inferences. As it is not known which of the multiple haplotype inferences are best supported by the given unphased data set, we use a bootstrapping procedure that allows for scoring of alternative inferences. Finally, by means of the bootstrapping scores, it is possible to optimise the phased genotypes belonging to a given haplotype inference. The program is evaluated with simulated and experimental SNP data generated for heterozygous tetraploid populations of potato. We show that, instead of taking the first haplotype inference reported by the program, we can significantly improve the quality of the final result by applying additional methods that include scoring of the alternative haplotype inferences and genotype optimisation. For a sub-population of nineteen individuals, the predicted results computed by SATlotyper were directly compared with results obtained by experimental haplotype inference via sequencing of cloned amplicons. Prediction and experiment gave similar results regarding the inferred haplotypes and phased genotypes.
Conclusion: Our results suggest that Haplotype Inference by Pure Parsimony can be solved efficiently by the SAT approach, even for data sets of unphased SNP from heterozygous polyploids. SATlotyper is freeware and is distributed as a Java JAR file. The software can be downloaded from the webpage of the GABI Primary Database at http://www.gabipd.org/projects/satlotyper/. The application of SATlotyper will provide haplotype information, which can be used in haplotype association mapping studies of polyploid plants.
In biological cells, the long-range intracellular traffic is powered by molecular motors which transport various cargos along microtubule filaments. The microtubules possess an intrinsic direction, having a 'plus' and a 'minus' end. Some molecular motors such as cytoplasmic dynein walk to the minus end, while others such as conventional kinesin walk to the plus end. Cells typically have an isopolar microtubule network. This is most pronounced in neuronal axons or fungal hyphae. In these long and thin tubular protrusions, the microtubules are arranged parallel to the tube axis with the minus ends pointing to the cell body and the plus ends pointing to the tip. In such a tubular compartment, transport by only one motor type leads to 'motor traffic jams'. Kinesin-driven cargos accumulate at the tip, while dynein-driven cargos accumulate near the cell body. We identify the relevant length scales and characterize the jamming behaviour in these tube geometries by using both Monte Carlo simulations and analytical calculations. A possible solution to this jamming problem is to transport cargos with a team of plus and a team of minus motors simultaneously, so that they can travel bidirectionally, as observed in cells. The presumably simplest mechanism for such bidirectional transport is provided by a 'tug-of-war' between the two motor teams which is governed by mechanical motor interactions only. We develop a stochastic tug-of-war model and study it with numerical and analytical calculations. We find a surprisingly complex cooperative motility behaviour. We compare our results to the available experimental data, which we reproduce qualitatively and quantitatively.
Inhalt: A. Einführung in das Thema und Vorstellung des SWP-Verbundes B. Motive der öffentlichen Hand für Public Private Partnerships C. Risiken für die öffentliche Hand D. Konkrete Auswirkungen der Teil-Privatisierung des Wasserbetriebes in Potsdam E. Warum eine Rekommunalisierung oftmals geboten erscheint F. Die Public Private Partnership im Tagesgeschäft G. Erfolgsfaktoren – wann PPPs auch für die öffentliche Hand und somit den Bürger erfolgreich sein können
Inhalt: A. Praktischer und theoretischer Entwicklungsstand von PPP in Deutschland B. Charakterisierung von PPP I. Grundkategorien von PPP II. Phasenmodell der Projektänderung und Kostensteigerungen bei PP-Projekten C. Wirtschaftlichkeitsanalyse und -vergleich I. Allgemeine Kennzeichnung II. Mögliche Gründe einer Vorteilhaftigkeit von PPP III. Risikoanalyse und Risikoverhalten als zentrales Problem des Wirtschaftlichkeitsvergleichs D. Transparenzbericht zur Deckung des Bedarfs an Informationen über Projektstruktur und -verlauf E. Schlussfolgerung und Entwicklungsperspektiven
Der Wettbewerbliche Dialog
(2008)
Inhalt: A. Einführung in die Thematik B. Bedeutung des Vergaberechts innerhalb des PPP-Beschaffungsprozesses C. Der Wettbewerbliche Dialog als „Dritter Weg“ zwischen offenem und Verhandlungsverfahren I. Anwendungsbereich II. Der Verfahrensablauf 1. Bekanntmachung 2. Präqualifikation der Anbieter 3. Dialogphase 4. Angebotsabgabe 5. Angebotswertung und Zuschlagserteilung D. Verhältnis zu den anderen Vergabearten I. Abgrenzung zum Verhandlungsverfahren II. Abgrenzung zum offenen Verfahren E. Bewertung und Ausblick I. Gründe für das bisherige Akzeptanzdefizit II. Vergleich zum Verhandlungsverfahren F. Zusammenfassender Ausblick
Kooperationsverträge
(2008)
Ignorance and Vulnerability : the 2002 mulde flood in the city of Eilenburg (Saxony, Germany)
(2008)
Der Beitrag zeigt in einem ersten Schritt, dass die "Absurda comica" ein theologisches Gleichnis darstellt, in dem der König die Rolle Gottes, Pickelhäring die Rolle des Teufels und die Handwerker die Rolle des Menschen übernehmen. Schlüssel für dieses Gleichnis ist Lk 9.60 und Mt 8.22, wo Christus von seinen Jüngern fordert, "die Toten ihre Toten begraben zu lassen", d.h. sich vom Reich der Welt abzuwenden. Auf die "Absurda comica" übertragen, besagt dies, dass die Handwerker die Rollenhaftigkeit und die Narrheit ihrer Existenz erkennen müssen, wenn sie vor Gott gerechtfertigt werden wollen. In einem zweiten Schritt wird gezeigt, dass die "Absurda comica" als ein solches Gleichnis in der Tradition der Fastnachtspiele steht, deren Funktion eben dieser Aufweis der menschlichen Narrheit war. Das protestantische Schuldrama trat dabei bewusst in die Tradition dieser Fastnachtspiele, wie sich gerade an der "Absurda comica" zeigen lässt. In einem dritten Schritt wird diese These in einem Vergleich mit Jacob Masens "Rusticus imperans" und Christian Weises "Tobias und die Schwalbe" bestätigt.
Krieg in Text und Bild: Photographie in Barbusses "Le feu" und Célines "Voyage au bout de la nuit"
(2008)
"Le Feu" (1916) zählt zu den ersten, "Voyage au bout de la nuit" (1932) zu den letzten im Kontext des Ersten Weltkrieges entstandenen Romanen. Beide treten mit dem Anspruch auf zu sagen, wie es wirklich gewesen ist; beide nehmen Authentizität als Alleinstellungsmerkmal für sich in Anspruch. Dabei müssen sie scheinbar zwangsläufig den Umgang mit demjenigen Medium reflektieren, das per se für die unverstellte Darstellung der Wirklichkeit steht: die Photographie. Barbusse vertraut ihr und versucht, die Sprache der Frontsoldaten in maschineller Neutralität aufzunehmen. Céline hingegen postuliert eine Wahrheit, die hinter der Oberfläche des Photos liegt.Diese Stellungnahmen wirken sich auf die Erzählstrategien aus: Während Barbusse sich als gewissenhafter Transkribent der Mündlichkeit im Schützengraben gibt, behauptet Céline, allein die Brechung der (sprachlichen) Wirklichkeit durch die Schrift gebe deren Affektpotential wieder.Der medienvergleichende Ansatz fungiert in beiden Fällen als heuristisches Instrument zur Identifizierung ästhetischer Positionen.