Filtern
Volltext vorhanden
- nein (1) (entfernen)
Erscheinungsjahr
- 2008 (1) (entfernen)
Dokumenttyp
Sprache
- Englisch (1)
Gehört zur Bibliographie
- ja (1) (entfernen)
Institut
pH sensing in living cells represents one of the most prominent topics in biochemistry and physiology. In this study we performed one-photon and two-photon time-domain fluorescence lifetime imaging with a laser-scanning microscope using the time-correlated single-photon counting technique for imaging intracellular pH levels. The suitability of different commercial fluorescence dyes for lifetime-based pH sensing is discussed on the basis of in vitro as well of in situ measurements. Although the tested dyes are suitable for intensity-based ratiometric measurements, for lifetime- based techniques in the time-domain so far only BCECF seems to meet the requirements of reliable intracellular pH recordings in living cells.