Refine
Year of publication
Document Type
- Article (604)
- Preprint (299)
- Postprint (257)
- Conference Proceeding (160)
- Doctoral Thesis (51)
- Working Paper (48)
- Review (39)
- Monograph/Edited Volume (26)
- Part of a Book (24)
- Other (16)
Language
- English (1528) (remove)
Is part of the Bibliography
- no (1528) (remove)
Keywords
- Curriculum Framework (31)
- European values education (31)
- Europäische Werteerziehung (31)
- Familie (31)
- Family (31)
- Lehrevaluation (31)
- Studierendenaustausch (31)
- Unterrichtseinheiten (31)
- curriculum framework (31)
- lesson evaluation (31)
Institute
- Institut für Mathematik (309)
- Extern (270)
- Institut für Physik und Astronomie (119)
- Vereinigung für Jüdische Studien e. V. (119)
- Department Linguistik (91)
- Department Psychologie (86)
- Institut für Chemie (65)
- Hasso-Plattner-Institut für Digital Engineering GmbH (55)
- Historisches Institut (52)
- Institut für Umweltwissenschaften und Geographie (49)
Quantum dots (QDs) are common as luminescing markers for imaging in biological applications because their optical properties seem to be inert against their surrounding solvent. This, together with broad and strong absorption bands and intense, sharp tuneable luminescence bands, makes them interesting candidates for methods utilizing Forster Resonance Energy Transfer (FRET), e. g. for sensitive homogeneous fluoroimmunoassays (FIA). In this work we demonstrate energy transfer from Eu3+-trisbipyridin (Eu-TBP) donors to CdSe-ZnS-QD acceptors in solutions with and without serum. The QDs are commercially available CdSe-ZnS core-shell particles emitting at 655 nm (QD655). The FRET system was achieved by the binding of the streptavidin conjugated donors with the biotin conjugated acceptors. After excitation of Eu-TBP and as result of the energy transfer, the luminescence of the QD655 acceptors also showed lengthened decay times like the donors. The energy transfer efficiency, as calculated from the decay times of the bound and the unbound components, amounted to 37%. The Forster-radius, estimated from the absorption and emission bands, was ca. 77Å. The effective binding ratio, which not only depends on the ratio of binding pairs but also on unspecific binding, was obtained from the donor emission dependent on the concentration. As serum promotes unspecific binding, the overall FRET efficiency of the assay was reduced. We conclude that QDs are good substitutes for acceptors in FRET if combined with slow decay donors like Europium. The investigation of the influence of the serum provides guidance towards improving binding properties of QD assays.
To determine whether Förster resonance energy transfer (FRET) measurements can provide quantitative distance information in single-molecule fluorescence experiments on polypeptides, we measured FRET efficiency distributions for donor and acceptor dyes attached to the ends of freely diffusing polyproline molecules of various lengths. The observed mean FRET efficiencies agree with those determined from ensemble lifetime measurements but differ considerably from the values expected from Förster theory, with polyproline treated as a rigid rod. At donor–acceptor distances much less than the Förster radius R0, the observed efficiencies are lower than predicted, whereas at distances comparable to and greater than R0, they are much higher. Two possible contributions to the former are incomplete orientational averaging during the donor lifetime and, because of the large size of the dyes, breakdown of the point-dipole approximation assumed in Förster theory. End-to-end distance distributions and correlation times obtained from Langevin molecular dynamics simulations suggest that the differences for the longer polyproline peptides can be explained by chain bending, which considerably shortens the donor–acceptor distances.
A technique has been developed to measure absolute intracellular oxygen concentrations in green plants. Oxygen-sensitive phosphorescent microbeads were injected into the cells and an optical multifrequency phase-modulation technique was used to discriminate the sensor signal from the strong autofluorescence of the plant tissue. The method was established using photosynthesis-competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species.
Absorption and fluorescence properties of 4 hydraulic oils (3 biological and 1 petroleum-based) were investigated. In-situ LIF (laser-induced fluorescence) analysis of the oils on a brown sandy loam soil was performed. With calibration, quantitative detection was achieved. Estimated limits of detection were below ca. 500 mg/kg for the petroleum-based oil and ca. 2000 mg/kg for one biological oil. A semi-quantitative classification scheme is proposed for monitoring of the biological oils. This approach was applied to investigate the migration of a biological oil in soil-containing compartments, namely a soil column and a soil bed.
Results of an inter-laboratory round-robin study of the application of time-resolved emission spectroscopy (TRES) to the speciation of uranium(VI) in aqueous media are presented. The round-robin study involved 13 independent laboratories, using various instrumentation and data analysis methods. Samples were prepared based on appropriate speciation diagrams and, in general, were found to be chemically stable for at least six months. Four different types of aqueous uranyl solutions were studied: (1) acidic medium where UO22+aq is the single emitting species, (2) uranyl in the presence of fluoride ions, (3) uranyl in the presence of sulfate ions, and (4) uranyl in aqueous solutions at different pH, promoting the formation of hydrolyzed species. Results between the laboratories are compared in terms of the number of decay components, luminescence lifetimes, and spectral band positions. The successes and limitations of TRES in uranyl analysis and speciation in aqueous solutions are discussed.
Steady-state and time-resolved fluorescence methods were applied to investigate the fluorescence properties of humic substances of different origins. Using standard 2D emission and total luminescence spectra, fluorescence maxima, the width of the fluorescence band and a relative fluorescence quantum efficiency were determined. Different trends for fulvic acids and humic acids were observed indicating differences in the heterogeneity of the sample fractions. The complexity of the fluorescence decay of humic substances is discussed and compared to simple model compounds. The effect of oxidation of humic substances on their fluorescence properties is discussed as well.
In a recent contribution in Nature (vol. 442, pp. 555-558) Austin & Vivanco showed that sunlight is the dominant factor for decomposition of grass litter in a semi-arid grassland in Argentine. The quantification of this effect was portrayed as a novel finding. I put this result in the context of three other publications from as early as 1980 that quantified photodegradation. My synopsis shows that photodegradation is an important process in semi-arid grasslands in South America, North America and eastern Europe.
In the present study, photophysical properties of [N]phenylenes were studied by means of stationary and time-resolved absorption and fluorescence spectroscopy (in THF at room temperature). For biphenylene (1) and linear [3]phenylene (2a), internal conversion (IC) with quantum yields ΦIC > 0.99 is by far the dominant mechanism of S1 state deactivation. Angular [3]phenylene (3a), the zig-zag [4]- and [5]phenylenes (3b), (3c), and the triangular [4]phenylene (4) show fluorescence emission with fluorescence quantum yieds and lifetimes between ΦF = 0.07 for (3a) and 0.21 for (3c) and τF = 20 ns for (3a) and 81 ns for (4). Also, compounds (3) and (4) exhibit triplet formation upon photoexcitation with quantum yields as high as ΦISC = 0.45 for (3c). The strong differences in the fluorescence properties and in the triplet fromation efficiencies between (1) and (2a) on one hand and (3) and (4) on the other are related to the remarkable variation of the internal conversion (IC) rate constants kIC. A tentative classification of (1) and (2a) as “fast IC compounds”, with kIC > 109 s-1, and of (3) and (4) as “slow IC compounds”, with kIC ≈ 107 s-1, is suggested. This classification cannot simply be related to Hückel’s rule-type concepts of aromaticity, because the group of “fast IC compounds” consists of “antiaromatic” (1) and “aromatic” (2a), and the group of “slow IC compounds” consists of “antiaromatic” (3b), (4) and “aromatic” (3a), (3c). The IC in the [N]phenylenes is discussed within the framework of the so-called energy gap law established for non-radiative processes in benzenoid hydrocarbons.
The drift time spectra of polycyclic aromatic hydrocarbons (PAH), alkylbenzenes and alkylphenylethers were recorded with a laser-based ion mobility (IM) spectrometer. The ion mobilities of all compounds were determined in helium as drift gas. This allows the calculation of the diffusion cross sections (Omegacalc) on the basis of the exact hard sphere scattering model (EHSSM) and their comparison with the experimentally determined diffusion cross sections (Omegaexp). These Omegaexp/Omegacalc-correlations are presented for molecules with a rigid structure like PAH and prove the reliability of the theoretical model and experimental method. The increase of the selectivity of IM spectrometry is demonstrated using resonance enhanced multiphoton ionisation (REMPI) at atmospheric pressure, realized by tuneable lasers. The REMPI spectra of nine alkylbenzenes and alkylphenylethers are investigated. On the basis of these spectra, the complete qualitative distinction of eight compounds in a mixture is shown. These experiments are extended to alkylbenzene isomer mixtures.
Semiclassical asymptotics for the scattering amplitude in the presence of focal points at infinity
(2006)
We consider scattering in $\R^n$, $n\ge 2$, described by the Schr\"odinger operator $P(h)=-h^2\Delta+V$, where $V$ is a short-range potential. With the aid of Maslov theory, we give a geometrical formula for the semiclassical asymptotics as $h\to 0$ of the scattering amplitude $f(\omega_-,\omega_+;\lambda,h)$ $\omega_+\neq\omega_-$) which remains valid in the presence of focal points at infinity (caustics). Crucial for this analysis are precise estimates on the asymptotics of the classical phase trajectories and the relationship between caustics in euclidean phase space and caustics at infinity.
This paper focuses on some of the factors explaining recent trends in decentralisation, and some areas where decentralisation has had a positive impact, including bringing citizens into public affairs, improving sub-national public administration, and stimulating local economic development. It concludes by exploring the dangers and the implications for governments of differing capabilities starting out on the decentralisation path. More specifically, the paper stresses the underlying incentive structures within states in reform. It suggests a country-specific discussion of both vertical and horizontal incentive structures in decentralisation, as well as clear-cut accountability within a public sector in change. While vertical incentive structures mean defined rules for intergovernmental relationships, horizontal incentive structures mean defined rules between local governments, their citizens and the local private sector. Both sets of incentives need to be reformed jointly to stimulate better results from decentralisation and for better performance of local government. Neglecting one of them, could harm development. Above all, politics and processes are key to understanding, and eventually, managing decentralisation effectively.
The end of the cold war division of the Baltic Sea in 1989, and the three Baltic states’ return to independence in 1991 created new opportunities for the decision-makers of the area, as well as new possibilities for fashioning security in the region. This article will examine the security debate affecting the Baltic Sea region in the post-cold war period, and in particular, the relevance of the European Union to that debate. The following section will examine various concepts of security relevant to the Baltic region; the third section looks at the EU and the Baltic area; and the last part deals with the implications that EU membership by the Baltic Sea states may have for the security of the Baltic Sea zone.
The article mobilises the concept of strategic culture in order to identify the impact of history upon contemporary security policy. The article will first look at the "wholesale construction" of a strategic culture after the Second World War in West Germany before exploring its impact upon security policy since the end of the Cold War in two areas: the Bundeswehr's out-of-area role and conscription. The central argument presented here is that the strategic culture of the former Federal Republic now writ large on to the new united Germany sets the context within which security policies are designed. This strategic culture, as will be argued, acts as both a facilitating and a restraining variable on behaviour, making certain policy options possible and others impossible.
Observers of international politics have been conscious of the growing international involvement of non-central governments (NCGs), particularly in federal systems. These have been supplemented by the internationalisation of subnational actors in quasi-federal and even unitary states. One of the difficulties is that analysis has often been locked into the dominant paradigm debate in International Relations concerning who and who are not significant actors. Having briefly explored the nature of this changing environment, marked by a growing emphasis on access rather than control as a policy objective and the emergence of what is termed a 'catalytic diplomacy', the discussion focuses on the need for linkage between the levels of government in the pursuit of international as well as domestic policy goals. The nature of linkage mechanisms are discussed.
On the basis of the Dynamic Syntax framework, this paper argues that the production pressures in dialogue determining alignment effects and given versus new informational effects also drive the shift from case-rich free word order systems without clitic pronouns into systems with clitic pronouns with rigid relative ordering. The paper introduces assumptions of Dynamic Syntax, in particular the building up of interpretation through structural underspecification and update, sketches the attendant account of production with close coordination of parsing and production strategies, and shows how what was at the Latin stage a purely pragmatic, production-driven decision about linear ordering becomes encoded in the clitics in theMedieval Spanish system which then through successive steps of routinization yield the modern systems with immediately pre-verbal fixed clitic templates.
We analyze anaphoric phenomena in the context of building an input understanding component for a conversational system for tutoring mathematics. In this paper, we report the results of data analysis of two sets of corpora of dialogs on mathematical theorem proving. We exemplify anaphoric phenomena, identify factors relevant to anaphora resolution in our domain and extensions to the input interpretation component to support it.
Received views of utterance context in pragmatic theory characterize the occurrent subjective states of interlocutors using notions like common knowledge or mutual belief. We argue that these views are not compatible with the uncertainty and robustness of context-dependence in humanhuman dialogue. We present an alternative characterization of utterance context as objective and normative. This view reconciles the need for uncertainty with received intuitions about coordination and meaning in context, and can directly inform computational approaches to dialogue.
Goal-oriented dialog as a collaborative subordinated activity involving collective acceptance
(2006)
Modeling dialog as a collaborative activity consists notably in specifying the contain of the Conversational Common Ground and the kind of social mental state involved. In previous work (Saget, 2006), we claim that Collective Acceptance is the proper social attitude for modeling Conversational Common Ground in the particular case of goal-oriented dialog. We provide a formalization of Collective Acceptance, besides elements in order to integrate this attitude in a rational model of dialog are provided; and finally, a model of referential acts as being part of a collaborative activity is provided. The particular case of reference has been chosen in order to exemplify our claims.
A key problem for models of dialogue is to explain the mechanisms involved in generating and responding to clarification requests. We report a 'Maze task' experiment that investigates the effect of 'spoof' clarification requests on the development of semantic co-ordination. The results provide evidence of both local and global semantic co-ordination phenomena that are not captured by existing dialogue co-ordination models.
How does a shared lexicon arise in population of agents with differing lexicons, and how can this shared lexicon be maintained over multiple generations? In order to get some insight into these questions we present an ALife model in which the lexicon dynamics of populations that possess and lack metacommunicative interaction (MCI) capabilities are compared. We ran a series of experiments on multi-generational populations whose initial state involved agents possessing distinct lexicons. These experiments reveal some clear differences in the lexicon dynamics of populations that acquire words solely by introspection contrasted with populations that learn using MCI or using a mixed strategy of introspection and MCI. The lexicon diverges at a faster rate for an introspective population, eventually collapsing to one single form which is associated with all meanings. This contrasts sharply with MCI capable populations in which a lexicon is maintained, where every meaning is associated with a unique word. We also investigated the effect of increasing the meaning space and showed that it speeds up the lexicon divergence for all populations irrespective of their acquisition method.
Demonstratives, in particular gestures that "only" accompany speech, are not a big issue in current theories of grammar. If we deal with gestures, fixing their function is one big problem, the other one is how to integrate the representations originating from different channels and, ultimately, how to determine their composite meanings. The growing interest in multi-modal settings, computer simulations, human-machine interfaces and VRapplications increases the need for theories ofmultimodal structures and events. In our workshopcontribution we focus on the integration of multimodal contents and investigate different approaches dealing with this problem such as Johnston et al. (1997) and Johnston (1998), Johnston and Bangalore (2000), Chierchia (1995), Asher (2005), and Rieser (2005).
A new method is used in an eye-tracking pilot experiment which shows that it is possible to detect differences in common ground associated with the use of minimally different types of indefinite anaphora. Following Richardson and Dale (2005), cross recurrence quantification analysis (CRQA) was used to show that the tandem eye movements of two Swedish-speaking interlocutors are slightly more coupled when they are using fully anaphoric indefinite expressions than when they are using less anaphoric indefinites. This shows the potential of CRQA to detect even subtle processing differences in ongoing discourse.
Verbal or visual? : How information is distributed across speech and gesture in spatial dialog
(2006)
In spatial dialog like in direction giving humans make frequent use of speechaccompanying gestures. Some gestures convey largely the same information as speech while others complement speech. This paper reports a study on how speakers distribute meaning across speech and gesture, and depending on what factors. Utterance meaning and the wider dialog context were tested by statistically analyzing a corpus of direction-giving dialogs. Problems of speech production (as indicated by discourse markers and disfluencies), the communicative goals, and the information status were found to be influential, while feedback signals by the addressee do not have any influence.
We describe an experiment to gather original data on geometrical aspects of pointing. In particular, we are focusing upon the concept of the pointing cone, a geometrical model of a pointing’s extension. In our setting we employed methodological and technical procedures of a new type to integrate data from annotations as well as from tracker recordings. We combined exact information on position and orientation with rater’s classifications. Our first results seem to challenge classical linguistic and philosophical theories of demonstration in that they advise to separate pointings from reference.
Classical SDRT (Asher and Lascarides, 2003) discussed essential features of dialogue like adjacency pairs or corrections and up-dating. Recent work in SDRT (Asher, 2002, 2005) aims at the description of natural dialogue. We use this work to model situated communication, i.e. dialogue, in which sub-sentential utterances and gestures (pointing and grasping) are used as conventional modes of communication. We show that in addition to cognitive modelling in SDRT, capturing mental states and speech-act related goals, special postulates are needed to extract meaning out of contexts. Gestural meaning anchors Discourse Referents in contextually given domains. Both sorts of meaning are fused with the meaning of fragments to get at fully developed dialogue moves. This task accomplished, the standard SDRT machinery, tagged SDRSs, rhetorical relations, the up-date mechanism, and the Maximize Discourse Coherence constraint generate coherent structures. In sum, meanings from different verbal and non-verbal sources are assembled using extended SDRT to form coherent wholes.
We present a formal analysis of iconic coverbal gesture. Our model describes the incomplete meaning of gesture that’s derivable from its form, and the pragmatic reasoning that yields a more specific interpretation. Our formalism builds on established models of discourse interpretation to capture key insights from the descriptive literature on gesture: synchronous speech and gesture express a single thought, but while the form of iconic gesture is an important clue to its interpretation, the content of gesture can be resolved only by linking it to its context.
In two experiments, many annotators marked antecedents for discourse deixis as unconstrained regions of text. The experiments show that annotators do converge on the identity of these text regions, though much of what they do can be captured by a simple model. Demonstrative pronouns are more likely than definite descriptions to be marked with discourse antecedents. We suggest that our methodology is suitable for the systematic study of discourse deixis.
We present a new analysis of illocutionary forces in dialogue. We analyze them as complex conversational moves involving two dimensions: what Speaker commits herself to and what she calls on Addressee to perform. We start from the analysis of speech acts such as confirmation requests or whimperatives, and extend the analysis to seemingly simple speech acts, such as statements and queries. Then, we show how to integrate our proposal in the framework of the Grammar for Conversation (Ginzburg, to app.), which is adequate for modelling agents' information states and how they get updated.
Claiming that cross-speaker "but" can signal correction in dialogue, we start by describing the types of corrections "but" can communicate by focusing on the Speech Act (SA) communicated in the previous turn and address the ways in which "but" can correct what is communicated. We address whether "but" corrects the proposition, the direct SA or the discourse relation communicated in the previous turn. We will also briefly address other relations signalled by cross-turn "but". After presenting a typology of the situations "but" can correct, we will address how these corrections can be modelled in the Information State model of dialogue, motivating this work by showing how it can be used to potentially avoid misunderstandings. We wrap up by showing how the model presented here updates beliefs in the Information State representation of the dialogue and can be used to facilitate response deliberation.
An account is presented of the focus properties, common ground effect and dialogue behaviour of the accented German discourse marker "doch" and the accented sentence negation "nicht". It is argued that "doch" and "nicht" evoke as a focus alternative the logical complement of the proposition expressed by the sentence in which they occur, and that an analysis in terms of contrastive focus accounts for their effect on the common ground and their function in dialogue.
The performance of a home-built tunable diode laser (TDL) spectrometer, aimed at multi-line detection of carbon dioxide, has been evaluated and optimized. In the regime of the (30<SUP>0</SUP>1)<SUB>III</SUB> / (000) band of <SUP>12</SUP>CO<SUB>2</SUB> around 1.6 μm, the dominating isotope species <SUP>12</SUP>CO<SUB>2</SUB>, <SUP>13</SUP>CO<SUB>2</SUB>, and <SUP>12</SUP>C<SUP>18</SUP>O<SUP>16</SUP>O were detected simultaneously without interference by water vapor. Detection limits in the range of few ppmv were obtained for each species utilizing wavelength modulation (WM) spectroscopy with balanced detection in a long-path absorption cell set-up. High sensitivity in conjunction with high precision —typically ±1‰ and ±6‰ for 3% and 0.7% of CO<SUB>2</SUB>, respectively— renders this experimental approach a promising analytical concept for isotope-ratio determination of carbon dioxide in soil and breath gas. For a moderate <SUP>12</SUP>CO<SUB>2</SUB> line, the pressure dependence of the line profile was characterized in detail, to account for pressure effects on sensitive measurements.
Improvement of a fluorescence immunoassay with a compact diode-pumped solid state laser at 315 nm
(2006)
We demonstrate the improvement of fluorescence immunoassay (FIA) diagnostics in deploying a newly developed compact diode-pumped solid state (DPSS) laser with emission at 315 nm. The laser is based on the quasi-three-level transition in Nd:YAG at 946 nm. The pulsed operation is either realized by an active Q-switch using an electro-optical device or by introduction of a Cr<SUP>4+</SUP>:YAG saturable absorber as passive Q-switch element. By extra-cavity second harmonic generation in different nonlinear crystal media we obtained blue light at 473 nm. Subsequent mixing of the fundamental and the second harmonic in a β-barium-borate crystal provided pulsed emission at 315 nm with up to 20 μJ maximum pulse energy and 17 ns pulse duration. Substitution of a nitrogen laser in a FIA diagnostics system by the DPSS laser succeeded in considerable improvement of the detection limit. Despite significantly lower pulse energies (7 μJ DPSS laser versus 150 μJ nitrogen laser), in preliminary investigations the limit of detection was reduced by a factor of three for a typical FIA.
The performance of a home-built tunable diode laser (TDL) spectrometer has been optimized regarding multi-line detection of carbon dioxide in natural gases. In the regime of the (30<SUP>0</SUP>1)<SUB>III</SUB> ← (000) band of <SUP>12</SUP>CO<SUB>2</SUB> around 1.6 μm, the dominating isotope species <SUP>12</SUP>CO<SUB>2</SUB>, <SUP>13</SUP>CO<SUB>2</SUB>, and <SUP>12</SUP>C<SUP>18</SUP>O<SUP>16</SUP>O were detected simultaneously. In contrast to most established techniques, selective measurements are performed without any sample preparation. This is possible since the CO<SUB>2</SUB> detection is free of interference from water, ubiquitous in natural gases. Detection limits in the range of a few ppmv were obtained for each species utilizing wavelength modulation (WM) spectroscopy with balanced detection in a long-path absorption cell set-up. Linear calibration plots cover a dynamic range of four orders of magnitude, allowing for quantitative CO<SUB>2</SUB> detection in various samples, like soil and breath gas. High isotopic resolution enables the excellent selectivity, sensitivity, and stability of the chosen analytical concept. The obtained isotopic resolution of typically ± 1.0 ‰ and ± 1.5 ‰ (for 3 vol. % and 0.7 vol. % of CO<SUB>2</SUB>, respectively) offers a promising analytical tool for isotope-ratio determination of carbon dioxide in soil gas. Preliminary experiments on soil respiration for the first time combine the on-line quantification of the overall carbon dioxide content with an optode sensor and isotopic determination (TDL system) of natural gas species.
Near-infrared (NIR) absorption spectroscopy with tunable diode lasers allows the simultaneous detection of the three most important isotopologues of carbon dioxide (<SUP>12</SUP>CO<SUB>2</SUB>, <SUP>13</SUP>CO<SUB>2</SUB>, <SUP>12</SUP>C<SUP>18</SUP>O<SUP>16</SUP>O) and carbon monoxide (<SUP>12</SUP>CO, <SUP>13</SUP>CO, <SUP>12</SUP>C<SUP>18</SUP>O). The flexible and compact fiber-optic tunable diode laser absorption spectrometer (TDLAS) allows selective measurements of CO<SUB>2</SUB> and CO with high isotopic resolution without sample preparation since there is no interference with water vapour. For each species, linear calibration plots with a dynamic range of four orders of magnitude and detection limits (LOD) in the range of a few ppm were obtained utilizing wavelength modulation spectroscopy (WMS) with balanced detection in a Herriott-type multipass cell. The high performance of the apparatus is illustrated by fill-evacuation-refill cycles.
Two examples of our biophotonic research utilizing nanoparticles are presented, namely laser-based fluoroimmuno analysis and in-vivo optical oxygen monitoring. Results of the work include significantly enhanced sensitivity of a homogeneous fluorescence immunoassay and markedly improved spatial resolution of oxygen gradients in root nodules of a legume species.
We present an analysis of student language input in a corpus of tutoring dialogue in the domain of symbolic differentiation. Our focus on procedural tutoring makes the dialogue comparable to collaborative problem-solving (CPS). Existing CPS models describe the process of negotiating plans and goals, which also fits procedural tutoring. However, we provide a classification of student utterances and corpus annotation which shows that approximately 28% of non-trivial student language in this corpus is not accounted for by existing models, and addresses other functions, such as evaluating past actions or correcting mistakes. Our analysis can be used as a foundation for improving models of tutoring dialogue.
We present an extension to a comprehensive context model that has been successfully employed in a number of practical conversational dialogue systems. The model supports the task of multimodal fusion as well as that of reference resolution in a uniform manner. Our extension consists of integrating implicitly mentioned concepts into the context model and we show how they serve as candidates for reference resolution.
Islamic movements in Iran
(2004)
The modernist Islamic Movement sought to reconcile modern values and Islamic faith and attempted to express these values through an Islamic discourse and to reform political, religious and educational institutions along modernist lines. However, such a movement in the Islamic Republic of Iran raised controversy among the traditional leadership and secular intellectual groups. The aim of this paper is to discuss how far modernist Islam could progress in an islamic republic with an old tradition.
Quantum dots (QDs) are common as luminescing markers for imaging in biological applications because their optical properties seem to be inert against their surrounding solvent. This, together with broad and strong absorption bands and intense, sharp tuneable luminescence bands, makes them interesting candidates for methods utilizing Förster Resonance Energy Transfer (FRET), e. g. for sensitive homogeneous fluoroimmunoassays (FIA). In this work we demonstrate energy transfer from Eu<SUP>3+</SUP>-trisbipyridin (Eu-TBP) donors to CdSe-ZnS-QD acceptors in solutions with and without serum. The QDs are commercially available CdSe-ZnS core-shell particles emitting at 655 nm (QD655). The FRET system was achieved by the binding of the streptavidin conjugated donors with the biotin conjugated acceptors. After excitation of Eu-TBP and as result of the energy transfer, the luminescence of the QD655 acceptors also showed lengthened decay times like the donors. The energy transfer efficiency, as calculated from the decay times of the bound and the unbound components, amounted to 37%. The Förster-radius, estimated from the absorption and emission bands, was ca. 77 Å. The effective binding ratio, which not only depends on the ratio of binding pairs but also on unspecific binding, was obtained from the donor emission dependent on the concentration. As serum promotes unspecific binding, the overall FRET efficiency of the assay was reduced. We conclude that QDs are good substitutes for acceptors in FRET if combined with slow decay donors like Europium. The investigation of the influence of the serum provides guidance towards improving binding properties of QD assays.
Face-to-face communication is multimodal. In unscripted spoken discourse we can observe the interaction of several "semiotic layers", modalities of information such as syntax, discourse structure, gesture, and intonation. We explore the role of gesture and intonation in structuring and aligning information in spoken discourse through a study of the co-occurrence of pitch accents and gestural apices. Metaphorical spatialization through gesture also plays a role in conveying the contextual relationships between the speaker, the government and other external forces in a naturally-occurring political speech setting.
The present study examines native and nonnative perceptual processing of semantic information conveyed by prosodic prominence. Five groups of German learners of English each listened to one of 5 experimental conditions. Three conditions differed in place of focus accent in the sentence and two conditions were with spliced stimuli. The experiment condition was presented first in the learners’ L1 (German) and then in a similar set in the L2 (English). The effect of the accent condition and of the length and position of the target in the sentence was evaluated in a probe recognition task. In both the L1 and L2 tasks there was no significant effect in any of the five focus conditions. Target position and target word length had an effect in the L1 task. Word length did not affect accuracy rates in the L2 task. For probe recognition in the L2, word length and the position of the target interacted with the focus condition.
This paper investigates the structural properties of morphosyntactically marked focus constructions, focussing on the often neglected non-focal sentence part in African tone languages. Based on new empirical evidence from five Gur and Kwa languages, we claim that these focus expressions have to be analysed as biclausal constructions even though they do not represent clefts containing restrictive relative clauses. First, we relativize the partly overgeneralized assumptions about structural correspondences between the out-of-focus part and relative clauses, and second, we show that our data do in fact support the hypothesis of a clause coordinating pattern as present in clause sequences in narration. It is argued that we deal with a non-accidental, systematic feature and that grammaticalization may conceal such basic narrative structures.
The Semantics of Ellipsis
(2005)
There are four phenomena that are particularly troublesome for theories of ellipsis: the existence of sloppy readings when the relevant pronouns cannot possibly be bound; an ellipsis being resolved in such a way that an ellipsis site in the antecedent is not understood in the way it was there; an ellipsis site drawing material from two or more separate antecedents; and ellipsis with no linguistic antecedent. These cases are accounted for by means of a new theory that involves copying syntactically incomplete antecedent material and an analysis of silent VPs and NPs that makes them into higher order definite descriptions that can be bound into.
Stop bashing givenness!
(2005)
Elke Kasimir’s paper (in this volume) argues against employing the notion of Givenness in the explanation of accent assignment. I will claim that the arguments against Givenness put forward by Kasimir are inconclusive because they beg the question of the role of Givenness. It is concluded that, more generally, arguments against Givenness as a diagnostic for information structural partitions should not be accepted offhand, since the notion of Givenness of discourse referents is (a) theoretically simple, (b) readily observable and quantifiable, and (c) bears cognitive significance.
In order to investigate the empirical properties of focus, it is necessary to diagnose focus (or: "what is focused") in particular linguistic examples. It is often taken for granted that the application of one single diagnostic tool, the so-called question-answer test, which roughly says that whatever a question asks for is focused in the answer, is a fool-proof test for focus. This paper investigates one example class where such uncritical belief in the question-answer test has led to the assumption of rather complex focus projection rules: in these examples, pitch accent placement has been claimed to depend on certain parts of the focused constituents being given or not. It is demonstrated that such focus projection rules are unnecessarily complex and in turn require the assumption of unnecessarily complicated meaning rules, not to speak of the difficulties to give a precise semantic/pragmatic definition of the allegedly involved givenness property. For the sake of the argument, an alternative analysis is put forward which relies solely on alternative sets following Mats Rooth's work, and avoids any recourse to givenness. As it turns out, this alternative analysis is not only simpler but also makes in a critical case the better predictions.
We present a system for the linguistic exploration and analysis of lexical cohesion in English texts. Using an electronic thesaurus-like resource, Princeton WordNet, and the Brown Corpus of English, we have implemented a process of annotating text with lexical chains and a graphical user interface for inspection of the annotated text. We describe the system and report on some sample linguistic analyses carried out using the combined thesaurus-corpus resource.
This paper discusses the use of XSLT stylesheets as a filtering mechanism for refining the results of user queries on treebanks. The discussion is within the context of the TIGER treebank, the associated search engine and query language, but the general ideas can apply to any search engine for XML-encoded treebanks. It will be shown that important classes of linguistic phenomena can be accessed by applying relatively simple XSLT templates to the output of a query, effectively simulating the universal quantifier for a subset of the query language. uni-potsdam.de/cgi-bin/publika/view.pl?id=206">
Fronting of an infinite VP across a finite main verb-akin to German "VP-topicalization"-can be found also in Czech and Polish. The paper discusses evidence from large corpora for this process and some of its properties, both syntactic and information-structural. Based on this case, criteria for more user-friedly searching and retrieval of corpus data in syntactic research are being developed.
Multiple hierarchies
(2005)
In this paper, we present the Multiple Annotation approach, which solves two problems: the problem of annotating overlapping structures, and the problem that occurs when documents should be annotated according to different, possibly heterogeneous tag sets. This approach has many advantages: it is based on XML, the modeling of alternative annotations is possible, each level can be viewed separately, and new levels can be added at any time. The files can be regarded as an interrelated unit, with the text serving as the implicit link. Two representations of the information contained in the multiple files (one in Prolog and one in XML) are described. These representations serve as a base for several applications.
This paper describes the standardization problems that come up in a diachronic corpus: it has to cope with differing standards with regard to diplomaticity, annotation, and header information. Such highly het-erogeneous texts must be standardized to allow for comparative re-search without (too much) loss of information.
Unity in diversity
(2005)
This paper describes the creation and preparation of TUSNELDA, a collection of corpus data built for linguistic research. This collection contains a number of linguistically annotated corpora which differ in various aspects such as language, text sorts / data types, encoded annotation levels, and linguistic theories underlying the annotation. The paper focuses on this variation on the one hand and the way how these heterogeneous data are integrated into one resource on the other hand.
The paper is an enquiry into dynamic social contract theory. The social contract defines the rules of resource use. An intergenerational social contract in an economy with a single exhaustible resource is examined within a framework of an overlapping generations model. It is assumed that new generations do not accept the old social contract, and access to resources will be renegotiated between any incumbent generation and their successors. It turns out that later generations will be in an unfortunate position regardless of their bargaining power.
ANNIS
(2004)
In this paper, we discuss the design and implementation of our first version of the database "ANNIS" ("ANNotation of Information Structure"). For research based on empirical data, ANNIS provides a uniform environment for storing this data together with its linguistic annotations. A central database promotes standardized annotation, which facilitates interpretation and comparison of the data. ANNIS is used through a standard web browser and offers tier-based visualization of data and annotations, as well as search facilities that allow for cross-level and cross-sentential queries. The paper motivates the design of the system, characterizes its user interface, and provides an initial technical evaluation of ANNIS with respect to data size and query processing.
Focus strategies in chadic
(2004)
We argue that the standard focus theories reach their limits when confronted with the focus systems of the Chadic languages. The backbone of the standard focus theories consists of two assumptions, both called into question by the languages under consideration. Firstly, it is standardly assumed that focus is generally marked by stress. The Chadic languages, however, exhibit a variety of different devices for focus marking. Secondly, it is assumed that focus is always marked. In Tangale, at least, focus is not marked consistently on all types of constituents. The paper offers two possible solutions to this dilemma.
In this paper we review the current state of research on the issue of discourse structure (DS)/information structure (IS) interface. This field has received a lot of attention from discourse semanticists and pragmatists, and has made substantial progress in recent years. In this paper we summarize the relevant studies. In addition, we look at the issue of DS/ISinteraction at a different level - that of phonetics. It is known that both information structure and discourse structure can be realized prosodically, but the issue of phonetic interaction between the prosodic devices they employ has hardly ever been discussed in this context. We think that a proper consideration of this aspect of DS/IS-interaction would enrich our understanding of the phenomenon, and hence we formulate some related research-programmatic positions.
Prosody by phase
(2004)
Japanese wh-questions always exhibit focus intonation (FI). Furthermore, the domain of FI exhibits a correspondence to the wh-scope. I propose that this phonology-semantics correspondence is a result of the cyclic computation of FI, which is explained under the notion of Multiple Spell-Out in the recent Minimalist framework. The proposed analysis makes two predictions: (1) embedding of an FI into another is possible; (2) (overt) movement of a wh-phrase to a phase edge position causes a mismatch between FI and wh-scope. Both predictions are tested experimentally, and shown to be borne out.
We argue that there is a crucial difference between determiner and adverbial quantification. Following Herburger [2000] and von Fintel [1994], we assume that determiner quantifiers quantify over individuals and adverbial quantifiers over eventualities. While it is usually assumed that the semantics of sentences with determiner quantifiers and those with adverbial quantifiers basically come out the same, we will show by way of new data that quantification over events is more restricted than quantification over individuals. This is because eventualities in contrast to individuals have to be located in time which is done using contextual information according to a pragmatic resolution strategy. If the contextual information and the tense information given in the respective sentence contradict each other, the sentence is uninterpretable. We conclude that this is the reason why in these cases adverbial quantification, i.e. quantification over eventualities, is impossible whereas quantification over individuals is fine.
This paper investigates the nature of the attraction of XPs to clauseinitial position in German (and other languages). It argues that there are two different types of preposing. First, an XP can move when it is attracted by an EPP-like feature of Comp. Comp can, however, also attract elements that bear the formal marker of some semantic or pragmatic (information theoretic) function. This second type of movement is driven by the attraction of a formal property of the moved element. It has often been misanalysed as “operator” movement in the past. Japanese wh-questions always exhibit focus intonation (FI). Furthermore, the domain of FI exhibits a correspondence to the wh-scope. I propose that this phonology-semantics correspondence is a result of the cyclic computation of FI, which is explained under the notion of Multiple Spell-Out in the recent Minimalist framework. The proposed analysis makes two predictions: (1) embedding of an FI into another is possible; (2) (overt) movement of a wh-phrase to a phase edge position causes a mismatch between FI and wh-scope. Both predictions are tested experimentally, and shown to be borne out.
Results of a production experiment on the placement of sentence accent in German are reported. The hypothesis that German fulfills some of the most widely accepted rules of accent assignment— predicting focus domain integration—was only partly confirmed. Adjacency between argument and verb induces a single accent on the argument, as recognized in the literature, but interruption of this sequence by a modifier often induces remodeling of the accent pattern with a single accent on the modifier. The verb is rarely stressed. All models based on linear alignment or adjacency between elements belonging to a single accent domain fail to account for this result. A cyclic analysis of prosodic domain formation is proposed in an optimality-theoretic framework that can explain the accent pattern. Japanese wh-questions always exhibit focus intonation (FI). Furthermore, the domain of FI exhibits a correspondence to the wh-scope. I propose that this phonology-semantics correspondence is a result of the cyclic computation of FI, which is explained under the notion of Multiple Spell-Out in the recent Minimalist framework. The proposed analysis makes two predictions: (1) embedding of an FI into another is possible; (2) (overt) movement of a wh-phrase to a phase edge position causes a mismatch between FI and wh-scope. Both predictions are tested experimentally, and shown to be borne out.
The layer-by-layer assembly (LBL) of polyelectrolytes has been extensively studied for the preparation of ultrathin films due to the versatility of the build-up process. The control of the permeability of these layers is particularly important as there are potential drug delivery applications. Multilayered polyelectrolyte microcapsules are also of great interest due to their possible use as microcontainers. This work will present two methods that can be used as employable drug delivery systems, both of which can encapsulate an active molecule and tune the release properties of the active species. Poly-(N-isopropyl acrylamide), (PNIPAM) is known to be a thermo-sensitive polymer that has a Lower Critical Solution Temperature (LCST) around 32oC; above this temperature PNIPAM is insoluble in water and collapses. It is also known that with the addition of salt, the LCST decreases. This work shows Differential Scanning Calorimetry (DSC) and Confocal Laser Scanning Microscopy (CLSM) evidence that the LCST of the PNIPAM can be tuned with salt type and concentration. Microcapsules were used to encapsulate this thermo-sensitive polymer, resulting in a reversible and tunable stimuli- responsive system. The encapsulation of the PNIPAM inside of the capsule was proven with Raman spectroscopy, DSC (bulk LCST measurements), AFM (thickness change), SEM (morphology change) and CLSM (in situ LCST measurement inside of the capsules). The exploitation of the capsules as a microcontainer is advantageous not only because of the protection the capsules give to the active molecules, but also because it facilitates easier transport. The second system investigated demonstrates the ability to reduce the permeability of polyelectrolyte multilayer films by the addition of charged wax particles. The incorporation of this hydrophobic coating leads to a reduced water sensitivity particularly after heating, which melts the wax, forming a barrier layer. This conclusion was proven with Neutron Reflectivity by showing the decreased presence of D2O in planar polyelectrolyte films after annealing creating a barrier layer. The permeability of capsules could also be decreased by the addition of a wax layer. This was proved by the increase in recovery time measured by Florescence Recovery After Photobleaching, (FRAP) measurements. In general two advanced methods, potentially suitable for drug delivery systems, have been proposed. In both cases, if biocompatible elements are used to fabricate the capsule wall, these systems provide a stable method of encapsulating active molecules. Stable encapsulation coupled with the ability to tune the wall thickness gives the ability to control the release profile of the molecule of interest.
We analyze the notions of monotonicity and complete monotonicity for Markov Chains in continuous-time, taking values in a finite partially ordered set. Similarly to what happens in discrete-time, the two notions are not equivalent. However, we show that there are partially ordered sets for which monotonicity and complete monotonicity coincide in continuous time but not in discrete-time.
In semi-arid savannas, unsustainable land use can lead to degradation of entire landscapes, e.g. in the form of shrub encroachment. This leads to habitat loss and is assumed to reduce species diversity. In BIOTA phase 1, we investigated the effects of land use on population dynamics on farm scale. In phase 2 we scale up to consider the whole regional landscape consisting of a diverse mosaic of farms with different historic and present land use intensities. This mosaic creates a heterogeneous, dynamic pattern of structural diversity at a large spatial scale. Understanding how the region-wide dynamic land use pattern affects the abundance of animal and plant species requires the integration of processes on large as well as on small spatial scales. In our multidisciplinary approach, we integrate information from remote sensing, genetic and ecological field studies as well as small scale process models in a dynamic region-wide simulation tool. <hr> Interdisziplinäres Zentrum für Musterdynamik und Angewandte Fernerkundung Workshop vom 9. - 10. Februar 2006.
Interdisziplinäres Zentrum für Musterdynamik und Angewandte Fernerkundung Workshop vom 9. - 10. Februar 2006
Decisions for the conservation of biodiversity and sustainable management of natural resources are typically related to large scales, i.e. the landscape level. However, understanding and predicting the effects of land use and climate change on scales relevant for decision-making requires to include both, large scale vegetation dynamics and small scale processes, such as soil-plant interactions. Integrating the results of multiple BIOTA subprojects enabled us to include necessary data of soil science, botany, socio-economics and remote sensing into a high resolution, process-based and spatially-explicit model. Using an example from a sustainably-used research farm and a communally used and degraded farming area in semiarid southern Namibia we show the power of simulation models as a tool to integrate processes across disciplines and scales.
Interdisziplinäres Zentrum für Musterdynamik und Angewandte Fernerkundung Workshop vom 9. - 10. Februar 2006
Interdisziplinäres Zentrum für Musterdynamik und Angewandte Fernerkundung Workshop vom 9. - 10. Februar 2006
Interdisziplinäres Zentrum für Musterdynamik und Angewandte Fernerkundung Workshop vom 9. - 10. Februar 2006
Interdisziplinäres Zentrum für Musterdynamik und Angewandte Fernerkundung Workshop vom 9. - 10. Februar 2006
The rigorous development, application and validation of distributed hydrological models obligates to evaluate data in a spatially distributed way. In particular, spatial model predictions such as the distribution of soil moisture, runoff generating areas or nutrient-contributing areas or erosion rates, are to be assessed against spatially distributed observations. Also model inputs, such as the distribution of modelling units derived by GIS and remote sensing analyses, should be evaluated against groundbased observations of landscape characteristics. So far, however, quantitative methods of spatial field comparison have rarely been used in hydrology. In this paper, we present algorithms that allow to compare observed and simulated spatial hydrological data. The methods can be applied for binary and categorical data on regular grids. They comprise cell-by-cell algorithms, cell-neighbourhood approaches that account for fuzziness of location, and multi-scale algorithms that evaluate the similarity of spatial fields with changing resolution. All methods provide a quantitative measure of the similarity of two maps. The comparison methods are applied in two mountainous catchments in southern Germany (Brugga, 40 km<sup>2) and Austria (Löhnersbach, 16 km<sup>2). As an example of binary hydrological data, the distribution of saturated areas is analyzed in both catchments. For categorical data, vegetation zones that are associated with different runoff generation mechanisms are analyzed in the Löhnersbach. Mapped spatial patterns are compared to simulated patterns from terrain index calculations and from satellite image analysis. It is discussed how particular features of visual similarity between the spatial fields are captured by the quantitative measures, leading to recommendations on suitable algorithms in the context of evaluating distributed hydrological models.
Interdisziplinäres Zentrum für Musterdynamik und Angewandte Fernerkundung Workshop vom 9. - 10. Februar 2006
Integration of digital elevation models and satellite images to investigate geological processes.
(2006)
In order to better understand the geological boundary conditions for ongoing or past surface processes geologists face two important questions: 1) How can we gain additional knowledge about geological processes by analyzing digital elevation models (DEM) and satellite images and 2) Do these efforts present a viable approach for more efficient research. Here, we will present case studies at a variety of scales and levels of resolution to illustrate how we can substantially complement and enhance classical geological approaches with remote sensing techniques. Commonly, satellite and DEM based studies are being used in a first step of assessing areas of geologic interest. While in the past the analysis of satellite imagery (e.g. Landsat TM) and aerial photographs was carried out to characterize the regional geologic characteristics, particularly structure and lithology, geologists have increasingly ventured into a process-oriented approach. This entails assessing structures and geomorphic features with a concept that includes active tectonics or tectonic activity on time scales relevant to humans. In addition, these efforts involve analyzing and quantifying the processes acting at the surface by integrating different remote sensing and topographic data (e.g. SRTM-DEM, SSM/I, GPS, Landsat 7 ETM, Aster, Ikonos…). A combined structural and geomorphic study in the hyperarid Atacama desert demonstrates the use of satellite and digital elevation data for assessing geological structures formed by long-term (millions of years) feedback mechanisms between erosion and crustal bending (Zeilinger et al., 2005). The medium-term change of landscapes during hundred thousands to millions years in a more humid setting is shown in an example from southern Chile. Based on an analysis of rivers/watersheds combined with landscapes parameterization by using digital elevation models, the geomorphic evolution and change in drainage pattern in the coastal Cordillera can be quantified and put into the context of seismotectonic segmentation of a tectonically active region. This has far-reaching implications for earthquake rupture scenarios and hazard mitigation (K. Rehak, see poster on IMAF Workshop). Two examples illustrate short-term processes on decadal, centennial and millennial time scales: One study uses orogen scale precipitation gradients derived from remotely sensed passive microwave data (Bookhagen et al., 2005a). They demonstrate how debris flows were triggered as a response of slopes to abnormally strong rainfall in the interior parts of the Himalaya during intensified monsoons. The area of the orogen that receives high amounts of precipitation during intensified monsoons also constitutes numerous landslide deposits of up to 1km<sup>3 volume that were generated during intensified monsoon phase at about 27 and 9 ka (Bookhagen et al., 2005b). Another project in the Swiss Alps compared sets of aerial photographs recorded in different years. By calculating high resolution surfaces the mass transport in a landslide could be reconstructed (M. Schwab, Universität Bern). All these examples, although representing only a short and limited selection of projects using remote sense data in geology, have as a common approach the goal to quantify geological processes. With increasing data resolution and new sensors future projects will even enable us to recognize more patterns and / or structures indicative of geological processes in tectonically active areas. This is crucial for the analysis of natural hazards like earthquakes, tsunamis and landslides, as well as those hazards that are related to climatic variability. The integration of remotely sensed data at different spatial and temporal scales with field observations becomes increasingly important. Many of presently highly populated places and increasingly utilized regions are subject to significant environmental pressure and often constitute areas of concentrated economic value. Combined remote sensing and ground-truthing in these regions is particularly important as geologic, seismicity and hydrologic data may be limited here due to the recency of infrastructural development. Monitoring ongoing processes and evaluating the remotely sensed data in terms of recurrence of events will greatly enhance our ability to assess and mitigate natural hazards. <hr> Dokument 1: Foliensatz | Dokument 2: Abstract <hr> Interdisziplinäres Zentrum für Musterdynamik und Angewandte Fernerkundung Workshop vom 9. - 10. Februar 2006
Ergodicity of PCA
(2004)
For a general attractive Probabilistic Cellular Automata on S-Zd, we prove that the (time-) convergence towards equilibrium of this Markovian parallel dynamics, exponentially fast in the uniform norm, is equivalent to a condition (A). This condition means the exponential decay of the influence from the boundary for the invariant measures of the system restricted to finite boxes. For a class of reversible PCA dynamics on {1,+1}(Zd), wit a naturally associated Gibbsian potential rho, we prove that a (spatial-) weak mixing condition (WM) for rho implies the validity of the assumption (A); thus exponential (time-) ergodicity of these dynamics towards the unique Gibbs measure associated to rho hods. On some particular examples we state that exponential ergodicity holds as soon as there is no phase transition.
Existing theoretical literature fails to explain satisfactorily the differences between the pay of workers that are covered by collective agreements and others who are not. This study aims at providing a model framework which is amenable for an analysis of this issue. Our general-equilibrium approach integrates a dual labor market and a two-sector product market. The results suggest that the so-called 'union wage gap' is largely determined by the degree of centralization of the bargains, and, to a somewhat lesser extent, by the expenditure share of the unionized sector's goods.
Variation in nitrogen deposition and available soil nitrogen in a forest–grassland ecotone in Canada
(2004)
Regional variation in nitrogen (N) deposition increases plant productivity and decreases species diversity, but landscape- or local-scale influences on N deposition are less well-known. Using ion-exchange resin, we measured variation of N deposition and soil N availability within Elk Island National Park in the ecotone between grassland and boreal forest in western Canada. The park receives regionally high amounts of atmospheric N deposition (22 kg ha⁻¹ yr⁻¹). N deposition was on average higher ton clayrich luvisols than on brunisols, and areas burned 1 – 15 years previously received more atmospheric N than unburned sites. We suggest that the effects of previous fires and soil type on deposition rate act through differences in canopy structure. The magnitude of these effects varied with the presence of ungulate grazers (bison, moose, elk) and vegetation type (forest, shrubland, grassland). Available soil N (ammonium and nitrate) was higher in burned than unburned sites in the absence of grazing, suggesting an effect of deposition. On grazed sites, differences between fire treatments were small, presumably because the removal of biomass by grazers reduced the effect of fire. Aspen invades native grassland in this region, and our results suggest that fire without grazing might reinforce the expansion of forest into grassland facilitated by N deposition.
The interactions between peptides and lipids are of fundamental importance in the functioning of numerous membrane-mediated biochemical processes including antimicrobial peptide action, hormone-receptor interactions, drug bioavailability across the blood-brain barrier and viral fusion processes. Alteration of peptide structure could be a cause of many diseases. Biological membranes are complex systems, therefore simplified models may be introduced in order to understand processes occurring in nature. The lipid monolayers at the air/water interface are suitable model systems to mimic biological membranes since many parameters can be easily controlled. In the present work the lipid monolayers were used as a model membrane and their interactions with two different peptides B18 and Amyloid beta (1-40) peptide were investigated. B18 is a synthetic peptide that binds to lipid membranes that leads to the membrane fusion. It was demonstrated that it adopts different structures in the aqueous solutions and in the membrane interior. It is unstructured in solutions and forms alpha-helix at the air/water interface or in the membrane bound state. The peptide has affinity to the negatively charged lipids and even can fold into beta-sheet structure in the vicinity of charged membranes at high peptide to lipid ratio. It was elucidated that in the absence of electrostatic interactions B18 does not influence on the lipid structure, whereas it provides partial liquidization of the negatively charged lipids. The understanding of mechanism of the peptide action in model system may help to develop the new type of antimicrobial peptides as well as it can shed light on the general mechanisms of peptide/membrane binding. The other studied peptide - Amyloid beta (1-40) peptide, which is the major component of amyloid plaques found in the brain of patients with Alzheimer's disease. Normally the peptide is soluble and is not toxic. During aging or as a result of the disease it aggregates and shows a pronounced neurotoxicity. The peptide aggregation involves the conformational transition from a random coil or alpha-helix to beta-sheets. Recently it was demonstrated that the membrane can play a crucial role for the peptide aggregation and even more the peptide can cause the change in the cell membranes that leads to a neuron death. In the present studies the structure of the membrane bound Amyloid beta peptide was elucidated. It was found that the peptide adopts the beta-sheet structure at the air/water interface or being adsorbed on lipid monolayers, while it can form alpha-helical structure in the presence of the negatively charged vesicles. The difference between the monolayer system and the bulk system with vesicles is the peptide to lipid ratio. The peptide adopts the helical structure at low peptide to lipid ratio and folds into beta-sheet at high ratio. Apparently, Abeta peptide accumulation in the brain is concentration driven. Increasing concentration leads to a change in the lipid to peptide ratio that induces the beta-sheet formation. The negatively charged lipids can act as seeds in the plaque formation, the peptide accumulates on the membrane and when the peptide to lipid ratio increases it the peptide forms toxic beta-sheet containing aggregates.
We study a natural Dirac operator on a Lagrangian submanifold of a Kähler manifold. We first show that its square coincides with the Hodge - de Rham Laplacian provided the complex structure identifies the Spin structures of the tangent and normal bundles of the submanifold. We then give extrinsic estimates for the eigenvalues of that operator and discuss some examples.