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Das Dokument "Forschungsdatenmanagement bei personenbezogenen Daten - eine Handreichung" versammelt zentrale Inhalte, Verweise und Vorgehensweisen für Forscher*innen, die in einer Studie personenbezogene Daten erheben und diese verarbeiten, archivieren oder veröffentlichen wollen. Die Handreichung verweist an den entsprechenden Abschnitten auf weiterführende Materialien wie insbesondere die Handreichung „Datenschutz“ des Rats für die Sozial-, Verhaltens-, Bildungs- und Wirtschaftswissenschaften (RatSWD).
Sulfur is an important element that is incorporated into many biomolecules in humans. The incorporation and transfer of sulfur into biomolecules is, however, facilitated by a series of different sulfurtransferases. Among these sulfurtransferases is the human mercaptopyruvate sulfurtransferase (MPST) also designated as tRNA thiouridine modification protein (TUM1). The role of the human TUM1 protein has been suggested in a wide range of physiological processes in the cell among which are but not limited to involvement in Molybdenum cofactor (Moco) biosynthesis, cytosolic tRNA thiolation and generation of H2S as signaling molecule both in mitochondria and the cytosol. Previous interaction studies showed that TUM1 interacts with the L-cysteine desulfurase NFS1 and the Molybdenum cofactor biosynthesis protein 3 (MOCS3). Here, we show the roles of TUM1 in human cells using CRISPR/Cas9 genetically modified Human Embryonic Kidney cells. Here, we show that TUM1 is involved in the sulfur transfer for Molybdenum cofactor synthesis and tRNA thiomodification by spectrophotometric measurement of the activity of sulfite oxidase and liquid chromatography quantification of the level of sulfur-modified tRNA. Further, we show that TUM1 has a role in hydrogen sulfide production and cellular bioenergetics.
In late summer, migratory bats of the temperate zone face the challenge of accomplishing two energy-demanding tasks almost at the same time: migration and mating. Both require information and involve search efforts, such as localizing prey or finding potential mates. In non-migrating bat species, playback studies showed that listening to vocalizations of other bats, both con-and heterospecifics, may help a recipient bat to find foraging patches and mating sites. However, we are still unaware of the degree to which migrating bats depend on con-or heterospecific vocalizations for identifying potential feeding or mating opportunities during nightly transit flights. Here, we investigated the vocal responses of Nathusius’ pipistrelle bats, Pipistrellus nathusii, to simulated feeding and courtship aggregations at a coastal migration corridor. We presented migrating bats either feeding buzzes or courtship calls of their own or a heterospecific migratory species, the common noctule, Nyctalus noctula. We expected that during migratory transit flights, simulated feeding opportunities would be particularly attractive to bats, as well as simulated mating opportunities which may indicate suitable roosts for a stopover. However, we found that when compared to the natural silence of both pre-and post-playback phases, bats called indifferently during the playback of conspecific feeding sounds, whereas P. nathusii echolocation call activity increased during simulated feeding of N. noctula. In contrast, the call activity of P. nathusii decreased during the playback of conspecific courtship calls, while no response could be detected when heterospecific call types were broadcasted. Our results suggest that while on migratory transits, P. nathusii circumnavigate conspecific mating aggregations, possibly to save time or to reduce the risks associated with social interactions where aggression due to territoriality might be expected. This avoidance behavior could be a result of optimization strategies by P. nathusii when performing long-distance migratory flights, and it could also explain the lack of a response to simulated conspecific feeding. However, the observed increase of activity in response to simulated feeding of N. noctula, suggests that P. nathusii individuals may be eavesdropping on other aerial hawking insectivorous species during migration, especially if these occupy a slightly different foraging niche.
Obwohl Bildungsreisen nach England und in andere westeuropäische Staaten in adligen Kreisen seinerzeit regelrecht in Mode waren, ragen die Itzenplitz'schen Briefe aufgrund ihrer inhaltlichen Vielseitigkeit sowie wegen des häufigen Auftretens der bedeutendsten englischen Agrarökonomen und Naturwissenschaftler jener Zeit aus vergleichbaren Überlieferungen heraus.Henriette und Peter von Itzenplitz - erstere war die Tochter der vor allem durch Theodor Fontanes »Wanderungen...« bekannt gewordenen Agrarpionierin »Frau von Friedland« auf Kunersdorf im Oderbruch - traten kurz nach ihrer Hochzeit im Herbst 1792 eine landwirtschaftlich-ökonomisch-botanische Bildungsreise nach England an. Sie starteten vom Itzenplitz'schen Familiengut im havelländischen Groß Behnitz aus und sollten über ein Jahr lang unterwegs sein. Erst am letzten Tag des Jahres 1793 kehrte das junge Ehepaar mit ihrem in Kew bei London geborenen Sohn abgekämpft, aber erfüllt heim.Die von den beiden unterwegs regelmäßig an...
Chronologie der Englandreise
(2023)
Einleitender Kommentar
(2023)
Digitale Reflexionsanlässe
(2023)
Geschichts-Didaktik
(2023)
Its properties make copper one of the world’s most important functional metals. Numerous megatrends are increasing the demand for copper. This requires the prospection and exploration of new deposits, as well as the monitoring of copper quality in the various production steps. A promising technique to perform these tasks is Laser Induced Breakdown Spectroscopy (LIBS). Its unique feature, among others, is the ability to measure on site without sample collection and preparation. In this work, copper-bearing minerals from two different deposits are studied. The first set of field samples come from a volcanogenic massive sulfide (VMS) deposit, the second part from a stratiform sedimentary copper (SSC) deposit. Different approaches are used to analyze the data. First, univariate regression (UVR) is used. However, due to the strong influence of matrix effects, this is not suitable for the quantitative analysis of copper grades. Second, the multivariate method of partial least squares regression (PLSR) is used, which is more suitable for quantification. In addition, the effects of the surrounding matrices on the LIBS data are characterized by principal component analysis (PCA), alternative regression methods to PLSR are tested and the PLSR calibration is validated using field samples.
Das Eigene und das Fremde
(2023)
Die vorliegende Arbeit stellt eine Untersuchung des Fremdverstehens von Lehrkräften im Mathematikunterricht dar. Mit ‚Fremdverstehen‘ soll dabei – in Anlehnung an den Soziologen Alfred Schütz – der Prozess bezeichnet werden, in welchem eine Lehrkraft versucht, das Verhalten einer Schülerin oder eines Schülers zu verstehen, indem sie dieses Verhalten auf ein Erleben zurückführt, das ihm zugrunde gelegen haben könnte. Als ein wesentliches Merkmal des Prozesses stellt Schütz in seiner Theorie des Fremdverstehens heraus, dass das Fremdverstehen eines Menschen immer auch auf seinen eigenen Erlebnissen basiert. Aus diesem Grund wird in der Arbeit ein methodischer Zweischritt vorgenommen: Es werden zunächst die mathematikbezogenen Erlebnisse zweier Lehrkräfte nachgezeichnet, bevor dann ihr Fremdverstehen in konkreten Situationen im Mathematikunterricht rekonstruiert wird. In der ersten Teiluntersuchung (= der Rekonstruktion eigener Erlebnisse der untersuchten Lehrkräfte) erfolgt die Datenerhebung mit Hilfe biographisch-narrativer Interviews, in denen die untersuchten Lehrkräfte angeregt werden, ihre mathematikbezogene Lebensgeschichte zu erzählen. Die Analyse dieser Interviews wird im Sinne der rekonstruktiven Fallanalyse vorgenommen. Insgesamt führt die erste Teiluntersuchung zu textlichen Darstellungen der rekonstruierten mathematikbezogenen Lebensgeschichte der untersuchten Mathematiklehrkräfte. In der zweiten Teiluntersuchung (= der Rekonstruktion des Fremdverstehens der untersuchten Lehrkräfte) werden dann narrative Interviews geführt, in denen die untersuchten Lehrkräfte von ihrem Fremdverstehen in konkreten Situationen im Mathematikunterricht erzählen. Die Analyse dieser Interviews erfolgt mit Hilfe eines dreischrittigen Analyseverfahrens, welches die Autorin eigens zum Zweck der Rekonstruktion von Fremdverstehen entwickelte. Am Ende dieser zweiten Teiluntersuchung werden sowohl das rekonstruierte Fremdverstehen der Lehrkräfte in verschiedenen Unterrichtssituationen dargestellt als auch Strukturen, die sich in ihrem Fremdverstehen abzeichnen. Mit Hilfe einer theoretischen Verallgemeinerung werden schließlich – auf Basis der Ergebnisse der zweiten Teiluntersuchung – Aussagen über fünf Merkmale des Fremdverstehens von Lehrkräften im Mathematikunterricht im Allgemeinen gewonnen. Mit diesen Aussagen vermag die Arbeit eine erste Beschreibung davon hervorzubringen, wie sich das Phänomen des Fremdverstehens von Lehrkräften im Mathematikunterricht ausgestalten kann.
The African weakly electric fish genus Campylomormyrus includes 15 described species mostly native to the Congo River and its tributaries. They are considered sympatric species, because their distribution area overlaps. These species generate species-specific electric organ discharges (EODs) varying in waveform characteristics, including duration, polarity, and phase number. They exhibit also pronounced divergence in their snout, i.e. the length, thickness, and curvature. The diversifications in these two phenotypical traits (EOD and snout) have been proposed as key factors promoting adaptive radiation in Campylomormyrus. The role of EODs as a pre-zygotic isolation mechanism driving sympatric speciation by promoting assortative mating has been examined using behavioral, genetical, and histological approaches. However, the evolutionary effects of the snout morphology and its link to species divergence have not been closely examined. Hence, the main objective of this study is to investigate the effect of snout morphology diversification and its correlated EOD to better understand their sympatric speciation and evolutionary drivers. Moreover, I aim to utilize the intragenus and intergenus hybrids of Campylomormyrus to better understand trait divergence as well as underlying molecular/genetic mechanisms involved in the radiation scenario. To this end, I utilized three different approaches: feeding behavior analysis, diet assessment, and geometric morphometrics analysis. I performed feeding behavior experiments to evaluate the concept of the phenotype-environment correlation by testing whether Campylomormyrus species show substrate preferences. The behavioral experiments showed that the short snout species exhibits preference to sandy substrate, the long snout species prefers a stone substrate, and the species with intermediate snout size does not exhibit any substrate preference. The experiments suggest that the diverse feeding apparatus in the genus Campylomormyrus may have evolved in adaptation to their microhabitats. I also performed diet assessments of sympatric Campylomormyrus species and a sister genus species (Gnathonemus petersii) with markedly different snout morphologies and EOD using NGS-based DNA metabarcoding of their stomach contents. The diet of each species was documented showing that aquatic insects such as dipterans, coleopterans and trichopterans represent the major diet component. The results showed also that all species are able to exploit diverse food niches in their habitats. However, comparing the diet overlap indices showed that different snout morphologies and the associated divergence in the EOD translated into different prey spectra. These results further support the idea that the EOD could be a ‘magic trait’ triggering both adaptation and reproductive isolation. Geometric morphometrics method was also used to compare the phenotypical shape traits of the F1 intragenus (Campylomormyrus) and intergenus (Campylomormyrus species and Gnathonemus petersii) hybrids relative to their parents. The hybrids of these species were well separated based on the morphological traits, however the hybrid phenotypic traits were closer to the short-snouted species. In addition, the likelihood that the short snout expressed in the hybrids increases with increasing the genetic distance of the parental species. The results confirmed that additive effects produce intermediate phenotypes in F1-hybrids. It seems, therefore, that morphological shape traits in hybrids, unlike the physiological traits, were not expressed straightforward.
Das Spannungsverhältnis zwischen Meinungsfreiheit und allgemeinem Persönlichkeitsrecht hat das Bundesverfassungsgericht seit Jahrzehnten und wiederholt beschäftigt. Insbesondere in den letzten drei Jahren hatte sich das Gericht erneut intensiv damit zu befassen, einen angemessenen Ausgleich zwischen den sich gegenüberstehenden Grundrechtspositionen zu formulieren. Der Beitrag analysiert überblicksartig, inwieweit es in seinen neueren Entscheidungen seine bisherige Rechtsprechung fortgeführt, ergänzt oder gar nachjustiert hat.
The chapter describes the German procedure for obtaining compensation for wrongful convictions. Besides specific cases of compensation for convictions based on subsequently repealed legislation and claims arising from general official liability, German law provides a mechanism for compensation, insofar as a conviction is eliminated or mitigated in a retrial in criminal proceedings. Still within this retrial, the criminal court is called upon to determine whether a claim exists for compensation of material and/or non-material damages on the merits. The amount of compensation is assessed in a judicial administrative procedure at the request of the person concerned. The compensation for non-material harm is calculated on the basis of a lump sum of currently €75 per day of imprisonment; the extent of material harm must be submitted and proven by the person concerned. She has the right to take legal action in the civil courts against the decision on the amount, she has the right to take legal action in the civil courts.
§§ 253-299a
(2023)
Die Kommentierung umfasst neben der Zivilprozessordnung auch die relevanten Nebengesetze (wie EGZPO, GVG, KapMuG und MediationsG) sowie das europäische und internationale Zivilprozessrecht. Selbstverständlich sind alle relevanten Gesetzesänderungen sowie die neuesten Entwicklungen in Rechtsprechung und Lehre berücksichtigt.
Umfassende Kommentierung zum Verfahren vor den Landgerichten bis zum Urteil.
Networking fast and slow
(2023)
Growing interest in network dynamics has led to insights about patterns of network change, drivers of tie formation, and the temporal unfolding of the consequences of networks. To this area of inquiry, we introduce networking speed—the time that it takes for individuals to form a network tie—as an important but so far largely overlooked aspect. We develop a theory of networking speed that explains how different catalysts enable professionals to introduce variation into the speed with which they form interpersonal network ties. We discuss how such variation in the speed with which ties have been formed influences relational outcomes and the network returns that these ties generate. This discussion illustrates that high networking speed can entail advantages as well as pitfalls. We also explore temporal implications of networking speed—for instance, the persistence of the effects of speed over time. Overall, we conceptualize networking speed as a constitutive element of how interpersonal networks function in professional settings, and we propose a future research program for the integration of this novel concept into organizational network research.
Digitalization, as well as sustainability, are gaining increased relevance and have attracted significant attention in research and practice. However, the research already published about this topic examining digitalization in the retail sector does not consider the acceptance of related innovations, nor their impact on sustainability. Therefore, this article critically analyzes the acceptance of customers towards digital technologies in fashion stores as well as their impact on sustainability in the textile industry. The comprehensive analysis of the literature and the current state of research provide the basis of this paper. Theoretical models, such as the Technology-Acceptance-Model (TAM) and the Unified Theory of Acceptance and Use of Technology 2 (UTAUT 2) enable the evaluation of expectations and acceptance, as well as the assessment of possible inhibitory factors for the subsequent descriptive and statistical examination of the acceptance of digital technologies in fashion stores. The research on this subject was examined in a quantitative way. The key findings show that customers do accept digital technologies in fashion stores. The final part of this contribution describes the innovative Digitalization 4 Sustainability Framework which shows that digital technologies at the point of sale (PoS) in fashion stores could have a positive impact on sustainability. Overall, this paper shows that it is particularly important for fashion stores to concentrate on their individual strengths and customer needs as well as to indicate a more sustainable way by using digital technologies, in order to achieve added value for the customers and to set themselves apart from the competition while designing a more sustainable future. Moreover, fashion stores should make it a point of their honor to harness the power of digitalization for sake of sustainability and economic value creation.
Achilles tendinopathy (AT) is a debilitating injury in athletes, especially for those engaged in repetitive stretch-shortening cycle activities. Clinical risk factors are numerous, but it has been suggested that altered biomechanics might be associated with AT. No systematic review has been conducted investigating these biomechanical alterations in specifically athletic populations. Therefore, the aim of this systematic review was to compare the lower-limb biomechanics of athletes with AT to athletically matched asymptomatic controls. Databases were searched for relevant studies investigating biomechanics during gait activities and other motor tasks such as hopping, isolated strength tasks, and reflex responses. Inclusion criteria for studies were an AT diagnosis in at least one group, cross-sectional or prospective data, at least one outcome comparing biomechanical data between an AT and healthy group, and athletic populations. Studies were excluded if patients had Achilles tendon rupture/surgery, participants reported injuries other than AT, and when only within-subject data was available.. Effect sizes (Cohen's d) with 95% confidence intervals were calculated for relevant outcomes. The initial search yielded 4,442 studies. After screening, twenty studies (775 total participants) were synthesised, reporting on a wide range of biomechanical outcomes. Females were under-represented and patients in the AT group were three years older on average. Biomechanical alterations were identified in some studies during running, hopping, jumping, strength tasks and reflex activity. Equally, several biomechanical variables studied were not associated with AT in included studies, indicating a conflicting picture. Kinematics in AT patients appeared to be altered in the lower limb, potentially indicating a pattern of “medial collapse”. Muscular activity of the calf and hips was different between groups, whereby AT patients exhibited greater calf electromyographic amplitudes despite lower plantar flexor strength. Overall, dynamic maximal strength of the plantar flexors, and isometric strength of the hips might be reduced in the AT group. This systematic review reports on several biomechanical alterations in athletes with AT. With further research, these factors could potentially form treatment targets for clinicians, although clinical approaches should take other contributing health factors into account. The studies included were of low quality, and currently no solid conclusions can be drawn.
The Tetrarchy as Ideology
(2023)
In this paper, we show how socialist planning can be based on input-output data. We argue that the information required for this can be obtained by a central planning agency and thus dismiss Hayek’s information argument against socialism. We further show how economic planning can be made responsive to consumer demand through a feedback control mechanism. Output targets of products would be adjusted in response to observed consumer demand or based on predictions about future demand. Planners can use machine learning to make more accurate forecasts. The valuation of goods plays an important role in the feedback control mechanism. The values of goods can either be measured by the labour time necessary for their production (labour values) or through shadow prices based on linear programming.
Der generelle Prozess der Digitalisierung ist für die Medienordnung von besonderer Relevanz. Stehen auf der einen Seite wichtige Kommunikations- und Effektivitätsgewinne, so müssen auf der anderen Seite gesellschaftliche Gefahren durch Hatespeech, Fake News oder Urheberrechtsverletzungen erkannt und verhindert werden. Der vorliegende Band enthält die Vorträge, die auf der 1. Berlin-Potsdamer Konferenz zu interdisziplinären Rechtsfragen im März 2022 in Berlin gehalten wurden. Sie beleuchten die aus der Medienkonvergenz folgenden Veränderungen der Medienlandschaft in zentralen Bereichen wie der Bekämpfung von Desinformation, der Digitalwirtschaft, der Wissenskommunikation, dem Persönlichkeitsrechtsschutz, dem Influencer-Marketing, der Plattformregulierung, der Musikwirtschaft und der Anonymität in sozialen Netzwerken. Dabei geht es jeweils auch um Empfehlungen, welche politischen Aktivitäten erforderlich sind, um die Digitalisierung einerseits zu fördern, andererseits aber auch ihren Gefahren wirksam zu begegnen.
The Media Privilege under Data Protection Law: Journalism and data protection are fundamentally at odds with each other. In view of current developments, the need for a functioning regulatory concept for both legal positions is probably more important than ever. This conceptual balance is provided by the journalistic exemption in data protection law. The thesis focuses on the scope of the exception. It also identifies existing coherence problems between European and national law.
Investment control has experienced a considerable increase in importance in M&A transactions, as it has been constantly adapted to real economic conditions and successively tightened. The result is a partially inconsistent review regime that contains value contradictions and creates legal uncertainties. In a comprehensive approach, this doctoral thesis analyzes the problems of the current investment control regime and states concrete reform proposals.
Mitochondria and plastids are organelles with an endosymbiotic origin. During evolution, many genes are lost from the organellar genomes and get integrated in the nuclear genome, in what is known as intracellular/endosymbiotic gene transfer (IGT/EGT). IGT has been reproduced experimentally in Nicotiana tabacum at a gene transfer rate (GTR) of 1 event in 5 million cells, but, despite its centrality to eukaryotic evolution, there are no genetic factors known to influence the frequency of IGT in higher eukaryotes. The focus of this work was to determine the role of different DNA repair pathways of double strand break repair (DSBR) in the integration step of organellar DNA in the nuclear genome during IGT. Here, a CRISPR/Cas9 mutagenesis strategy was implemented in N. tabacum, with the aim of generating mutants in nuclear genes without expected visible phenotypes. This strategy led to the generation of a collection of independent mutants in the LIG4 (necessary for non-homologous end joining, NHEJ) and POLQ genes (necessary for microhomology mediated end joining, MMEJ). Targeting of other DSBR genes (KU70, KU80, RPA1C) generated mutants with unexpectedly strong developmental phenotypes.. These factors have telomeric roles, hinting towards a possible relationship between telomere length, and strength of developmental disruption upon loss of telomere structure in plants. The mutants were made in a genetic background encoding a plastid-encoded IGT reporter, that confers kanamycin resistance upon transfer to the nucleus. Through large scale independent experiments, increased IGT from the chloroplast to the nucleus was observed in lig4 mutants, as well as lines encoding a POLQ gene with a defective polymerase domain (polqΔPol). This shows that NHEJ or MMEJ have a double-sided relationship with IGT: while transferred genes may integrate using either pathway, the presence of both pathways suppresses IGT in wild-type somatic cells, thus demonstrating for the first time the extent on which nuclear genes control IGT frequency in plants. The IGT frequency increases in the mutants are likely mediated by increased availability of double strand breaks for integration. Additionally, kinetic analysis reveals that gene transfer (GT) events accumulate linearly as a function of time spent under antibiotic selection in the experiment, demonstrating that, contrary to what was previously thought, there is no such thing as a single GTR in somatic IGT experiments. Furthermore, IGT in tissue culture experiments appears to be the result of a "race against the clock" for integration in the nuclear genome, that starts when the organellar DNA arrives to the nucleus granting transient antibiotic resistance. GT events and escapes of kanamycin selection may be two possible outcomes from this race: those instances where the organellar DNA gets to integrate are recovered as GT events, and in those cases where timely integration fails, antibiotic resistance cannot be sustained, and end up considered as escapes. In the mutants, increased opportunities for integration in the nuclear genome change the overall ratio between IGT and escape events. The resources generated here are promising starting points for future research: (1) the mutant collection, for the further study of processes that depend on DNA repair in plants (2) the collection of GT lines obtained from these experiments, for the study of the effect of DSBR pathways over integration patterns and stability of transferred genes and (3) the developed CRISPR/Cas9 workflow for mutant generation, to make N. tabacum meet its potential as an attractive model for answering complex biological questions.
The G protein-coupled estrogen receptor (GPER1) is acknowledged as an important mediator of estrogen signaling. Given the ubiquitous expression of GPER1, it is likely that the receptor plays a role in a variety of malignancies, not only in the classic hormonally regulated tissues (e.g., breast, ovary, and prostate), but also in the colon. As colorectal cancer (CRC) is the third most common cancer in both men and women worldwide and environmental factors and dietary habits are important risk factors, it is increasingly recognized that natural and synthetic hormones and their associated receptors might play a role in CRC. Through oral consumption, environmental contaminants with endocrine activity are in contact with the gastrointestinal mucosa, where they might exert their toxic effects. Although GPER1 has been shown to be engaged in physiological and pathophysiological processes, its role in CRC remains poorly understood. Thus, pro- as well as anti-tumorigenic effects are described in the literature. This thesis has uncovered novel roles of GPER1 in mediating major CRC-associated phenotypes in transformed and non-transformed colon cell lines. Exposure to the estrogens 17β-estradiol (E2), bisphenol-A (BPA) and diethylstilbestrol (DES) but also the androgen dihydrotestosterone (DHT) resulted in GPER1-dependent induction of supernumerary centrosomes, whole chromosomal instability (w-CIN) and aneuploidy. Indeed, both knockdown and inhibition of GPER1 attenuated the generation of (xeno)hormone-driven supernumerary centrosomes and karyotype instability. Mechanistically, (xeno)hormone-induced centrosome amplification was associated with transient multipolar mitosis and the generation of so called anaphase “lagging” chromosomes. The results of this thesis propose a GPER1/PKA/AKAP9-pathway in regulating centrosome numbers in colorectal cancer cells and the involvement of the centriolar protein centrin. Remarkably, exposure to (xeno)hormones resulted in atypical enlargement and unexpected phosphorylation of the centriole marker centrin in interphase. These findings provide a novel role for GPER1 in key CRC-prone lesions and shed light on underlying mechanisms that involve GPER1 function in the colon. Elucidating to what extent centrosomal proteins are involved in the GPER1-mediated aneugenic effect will be an important task for future studies. The present study was intended to lay a first foundation to understand the molecular basis and potential risk factors of CRC which might help to reduce the use of laboratory animals. Since numerous animal experiments are conducted in biomedical research, the development of alternative methods is indispensable. The Federal Institute for Risk Assessment (BfR) as the German Center for the Protection of Laboratory Animals (Bf3R) addresses this issue by uncovering underlying mechanisms leading to colorectal cancer as necessary prerequisite in order to develop alternative methods.
This book offers a comprehensive, multidisciplinary introduction to theme parks and the field of theme park studies. It identifies and discusses relevant economic, social, and cultural as well as medial, historical, and geographical aspects of theme parks worldwide, from the big international theme park chains to smaller, regional, family-operated parks. The book also describes the theories and methods that have been used to study theme parks in various academic disciplines and reviews the major contexts in which theme parks have been studied. By providing the necessary backgrounds, theories, and methods to analyze and understand theme parks both as a business field and as a socio-cultural phenomenon, this book will be a great resource to students, academics from all disciplines interested in theme parks, and professionals and policy-makers in the leisure and entertainment as well as the urban planning sector.
The Tetrarchy as Ideology
(2023)
The 'Tetrarchy', the modern name assigned to the period of Roman history that started with the emperor Diocletian and ended with Constantine I, has been a much-studied and much-debated field of the Roman Empire. Debate, however, has focused primarily on whether it was a true 'system' of government, or rather a collection of ad-hoc measures undertaken to stabilise the empire after the troubled period of the 3rd century CE. The papers collected here aim to go beyond this question and to present an innovative approach to a fascinating period of Roman history by understanding the Tetrarchy not as a system of government, but primarily as a political language. Their focus thus lies on the language and ideology of the imperial college and court, on the performance of power in imperial ceremonies, the representation of the emperors and their enemies in the provinces of the Roman world, as well as on the afterlife of Tetrarchic power in the Constantinian period.
Die Fallsammlung ermöglicht zum einen eine exemplarische Wiederholung und Vertiefung des wirtschaftsstrafrechtlichen Basiswissens und zum anderen die Anwendung dieses Wissens auf ausbildungs- und praxisrelevante Sachverhalte. Einige Fälle weisen Bezüge zum Steuer- und Umweltstrafrecht auf, da diese Rechtsgebiete in manchen Ausbildungsordnungen mit dem Wirtschaftsstrafrecht verknüpft sind.Berücksichtigt wurden die zahlreichen Änderungen wirtschaftsstrafrechtlicher Gesetze sowie eine Fülle neuer gerichtlicher Entscheidungen und Stellungnahmen in der Literatur.
Verantwortungseigentum ist mittlerweile mehr als ein Nischenthema innerhalb der Rechtswissenschaft und unternehmerischen Praxis. Unternehmen werden in Verantwortungseigentum gegründet oder umgewandelt, also die persönlichen Gewinne der Gesellschafter perpetuiert und die Nachfolge reguliert. Auch der Profifußball stellt sich immer wieder die Frage nach der richtigen Balance zwischen Gewinnstreben und Gemeinwohl. Das wirft Fragen nach alternativen Gestaltungsmöglichkeiten auf, für die das Verantwortungseigentum eine Lösung bieten könnte.
Beinahe 30 Jahre lang glänzte die »Völkerfreundschaft« zwischen Kuba und der DDR im öffentlichen Diskurs der SED als Musterbeispiel ihres proletarischen Internationalismus. Doch die Rhetorik täuscht: Besonders in den Anfangsjahren der bilateralen Beziehungen nahmen die deutschen Kader ihre kubanischen »Genossen« in der Karibik als notorische Querschläger wahr, die mit ihrem Aufbegehren gegen den ideologischen Suprematieanspruch des Kremls die Stabilität des Ostblocks gefährdeten.
Anhand bislang unveröffentlichten Quellenmaterials aus deutschen und kubanischen Archiven veranschaulicht Antonia Bihlmayer die Bemühungen der Regierung Walter Ulbrichts, die widerspenstigen Sozialisten in der Karibik auf Moskau auszurichten. Auf politisch-ideologischer, wirtschaftlicher und kulturpolitischer Ebene analysiert ihre Studie zum einen die Charakteristika dieser sozialistischen Zivilisierungsmission. Zum anderen nimmt sie diejenigen Faktoren in den Blick, die dafür ausschlaggebend waren, dass sich diese beiden sozialistischen Enklaven ab Mitte der 1970er Jahre schließlich zu gleichwertigen »Juniorpartnern« der Sowjetunion entwickelten.
Photosynthesis converts light into metabolic energy which fuels plant growth. In nature, many factors influence light availability for photosynthesis on different time scales, from shading by leaves within seconds up to seasonal changes over months. Variability of light energy supply for photosynthesis can limit a plant´s biomass accumulation. Plants have evolved multiple strategies to cope with strongly fluctuation light (FL). These range from long-term optimization of leaf morphology and physiology and levels of pigments and proteins in a process called light acclimation, to rapid changes in protein activity within seconds. Therefore, uncovering how plants deal with FL on different time scales may provide key ideas for improving crop yield. Photosynthesis is not an isolated process but tightly integrates with metabolism through mutual regulatory interactions. We thus require mechanistic understanding of how long-term light acclimation shapes both, dynamic photosynthesis and its interactions with downstream metabolism. To approach this, we analyzed the influence of growth light on i) the function of known rapid photosynthesis regulators KEA3 and VCCN1 in dynamic photosynthesis (Chapter 2-3) and ii) the interconnection of photosynthesis with photorespiration (PR; Chapter 4).
We approached topic (i) by quantifying the effect of different growth light regimes on photosynthesis and photoprotection by using kea3 and vccn1 mutants. Firstly, we found that, besides photosynthetic capacity, the activities of VCCN1 and KEA3 during a sudden high light phase also correlated with growth light intensity. This finding suggests regulation of both proteins by the capacity of downstream metabolism. Secondly, we showed that KEA3 accelerated photoprotective non-photochemical quenching (NPQ) kinetics in two ways: Directly via downregulating the lumen proton concentration and thereby de-activating pH-dependent NPQ, and indirectly via suppressing accumulation of the photoprotective pigment zeaxanthin.
For topic (ii), we analyzed the role of PR, a process which recycles a toxic byproduct of the carbon fixation reactions, in metabolic flexibility in a dynamically changing light environment. For this we employed the mutants hpr1 and ggt1 with a partial block in PR. We characterized the function of PR during light acclimation by tracking molecular and physiological changes of the two mutants. Our data, in contrast to previous reports, disprove a generally stronger physiological relevance of PR under dynamic light conditions. Additionally, the two different mutants showed pronounced and distinct metabolic changes during acclimation to a condition inducing higher photosynthetic activity. This underlines that PR cannot be regarded purely as a cyclic detoxification pathway for 2PG. Instead, PR is highly interconnected with plant metabolism, with GGT1 and HPR1 representing distinct metabolic modulators.
In summary, the presented work provides further insight into how energetic and metabolic flexibility is ensured by short-term regulators and PR during long-term light acclimation.
Parlamentarismus bezeichnet die Idee der Repräsentation Regierter durch gewählte Abgeordnete. Er entfaltete sich hierzulande seit dem frühen 19. Jahrhundert im Zusammenwirken mit Vereinen und Verbänden, Parteien und Bewegungen, innerhalb komplementärer oder auch konkurrierender Strukturen und Prinzipien. Das Selbst- und Fremdbild, die Zusammensetzung, Themen und Debattenkultur deutscher Parlamente als politische Akteure bildet damit bis heute zugleich eine Art Resonanzboden gesellschaftlicher und politischer Verhältnisse.
Welcher Weg führte von den zunächst kaum wirkmächtigen und nicht im Ansatz repräsentativen Parlamenten der Restaurationszeit über die Paulskirche bis in die Gegenwart? Wie gestalteten sich die Beziehungen zwischen dem Parlamentarismus und dem Föderalismus? Wie entwickelten sich Wahlen und Wahlsysteme als Grundlage allgemeiner Repräsentation? Wer sah oder sieht sich im Parlamentarismus in-, wer exkludiert? Wie leben und arbeiten Parlamentsangehörige ehedem und heute? Welche Formen medialer Interaktion zwischen ihnen und der Öffentlichkeit haben sich herausgebildet? Worin gründen Vorbehalte, Reformwünsche, aber auch Anfeindungen des Parlamentarismus? Und welchen Herausforderungen wird sich die repräsentative Demokratie in Zukunft stellen müssen?
Funken
(2023)
Bereits im vorschulischen Bereich, aber vor allem in der Grundschule entwickeln Kinder wichtige Kompetenzen für spätere Bildungsabschlüsse. Doch die Kompe-tenzunterschiede zwischen den Schüler:innen sind bereits zu Beginn der Grund-schulzeit beträchtlich. Somit kommt den Lehrkräften die überaus wichtige Aufga-be zu allen Kindern den für sie besten Bildungsweg zu ermöglichen. Um dieser Herausforderung zu begegnen, müssen Diagnostik und Förderung im Unterricht Hand in Hand gehen. Deshalb wird die Diagnosekompetenz von Lehrkräften als wichtige Voraussetzung für gelingenden Unterricht angesehen. Diese Dissertation widmet sich nun eben dieser wichtigen Kompetenz. Dabei wird sie als mehrdimen-sionales Konstrukt angesehen, zu dem neben der Beurteilung von fachlichen Kompetenzen auch die Einschätzung des Leistungsstandes und die Schlussfolge-rung hinsichtlich notwendiger Förderung im Unterricht gehören. Anhand dreier Artikel sowie ergänzender theoretischer Betrachtungen wurde die Diagnosekompe-tenz hinsichtlich möglicher Einflussfaktoren, der Bedeutung für den Unterricht sowie für die Lehrer:innenbildung untersucht.
De/lirios
(2023)
En base al concepto de "de/lirio", que articula trastornos en la enunciación literaria en primera persona, por un lado, con la caracterización psicopatológica de este yo enunciativo, por otro lado, el estudio explora las "líricas desviadas" de Mario Levrero y Alberto Laiseca, y muestra como responden productivamente a problemáticas estéticas, éticas y ontológicas propias de la vuelta del milenio, en el Río de la Plata y más allá.
The light reactions of photosynthesis are carried out by a series of multiprotein complexes embedded in thylakoid membranes. Among them, photosystem I (PSI), acting as plastocyanin-ferderoxin oxidoreductase, catalyzes the final reaction. Together with light-harvesting antenna I, PSI forms a high-molecular-weight supercomplex of ~600 kDa, consisting of eighteen subunits and nearly two hundred co-factors. Assembly of the various components into a functional thylakoid membrane complex requires precise coordination, which is provided by the assembly machinery. Although this includes a small number of proteins (PSI assembly factors) that have been shown to play a role in the formation of PSI, the process as a whole, as well as the intricacy of its members, remains largely unexplored.
In the present work, two approaches were used to find candidate PSI assembly factors. First, EnsembleNet was used to select proteins thought to be functionally related to known PSI assembly factors in Arabidopsis thaliana (approach I), and second, co-immunoprecipitation (Co-IP) of tagged PSI assembly factors in Nicotiana tabacum was performed (approach II).
Here, the novel PSI assembly factors designated CO-EXPRESSED WITH PSI ASSEMBLY 1 (CEPA1) and Ycf4-INTERACTING PROTEIN 1 (Y4IP1) were identified. A. thaliana null mutants for CEPA1 and Y4IP1 showed a growth phenotype and pale leaves compared with the wild type. Biophysical experiments using pulse amplitude modulation (PAM) revealed insufficient electron transport on the PSII acceptor side. Biochemical analyses revealed that both CEPA1 and Y4IP1 are specifically involved in PSI accumulation in A. thaliana at the post-translational level but are not essential. Consistent with their roles as factors in the assembly of a thylakoid membrane protein complex, the two proteins localize to thylakoid membranes. Remarkably, cepa1 y4ip1 double mutants exhibited lethal phenotypes in early developmental stages under photoautotrophic growth. Finally, co-IP and native gel experiments supported a possible role for CEPA1 and Y4IP1 in mediating PSI assembly in conjunction with other PSI assembly factors (e.g., PPD1- and PSA3-CEPA1 and Ycf4-Y4IP1). The fact that CEPA1 and Y4IP1 are found exclusively in green algae and higher plants suggests eukaryote-specific functions. Although the specific mechanisms need further investigation, CEPA1 and Y4IP1 are two novel assembly factors that contribute to PSI formation.
Social Scoring
(2023)
Das Fenster zum Garten
(2023)
Whistleblowing
(2023)
Taubblind, Juristin
(2023)
The Right to Research
(2023)
Refugees and displaced people rarely figure as historical actors, and almost never as historical narrators. We often assume a person residing in a refugee camp, lacking funding, training, social networks, and other material resources that enable the research and writing of academic history, cannot be a historian because a historian cannot be a person residing in a refugee camp.
The Right to Research disrupts this tautology by featuring nine works by refugee and host-community researchers from across Africa, Europe, and the Middle East. Identifying the intrinsic challenges of making space for diverse voices within a research framework and infrastructure that is inherently unequal, this edited volume offers a critical reflection on what history means, who narrates it, and what happens when those long excluded from authorship bring their knowledge and perspectives to bear. Chapters address topics such as education in Kakuma Refugee Camp, the political power of hip-hop in Rwanda, women migrants to Yemen, and the development of photojournalism in Kurdistan.
Exploring what it means to become a researcher, The Right to Research understands historical scholarship as an ongoing conversation - one in which we all have a right to participate.
Sin domicilio fijo
(2023)
Angesichts der dramatischen Lage in der Ukraine untersucht der folgende Beitrag, auf welchem Wege, vor welchen völkerrechtlichen Gerichten, in welchem Umfang und mit welcher Aussicht auf Erfolg die Ukraine oder einzelne ukrainische Staatsangehörige Sicherheitsschutz vor der russischen Invasion und/oder den im Zusammenhang damit bereits begangenen oder noch bevorstehenden Völkerrechtsverstößen Rechtsschutz erlangen können. Im Einzelnen handelt es sich hierbei um zwei anhängige Verfahren vor dem Internationalen Gerichtshof, mehrere Staaten- sowie eine große Vielzahl von Individualbeschwerden vor dem Europäischen Gerichtshof für Menschenrechte; ein Verfahren vor dem Internationalen Seegerichtshof; zahlreiche Investitionsverfahren vor internationalen Schiedsgerichten sowie schließlich zwei "Situationen" vor dem Internationalen Strafgerichtshof. Abschließend wird die Option der Schaffung eines ad-hoc-Tribunals für das Verbrechen der Aggression behandelt.
Wirtschafts- und Sozialverträglichkeit des Klimaschutzes als Verfassungsgebot und Staatsräson
(2023)
A right to research?
(2023)
Das menschenrechtliche Prinzip des Non-Refoulement vor den Vertragsorganen der Vereinten Nationen
(2023)
Die Vertragsorgane der Vereinten Nationen können durch die Abstimmung ihrer Praxis Rechtssicherheit schaffen, und zwar sowohl für Betroffene als auch für die Vertragsstaaten. Durch den ständigen Dialog mit den Vertragsstaaten, die Beeinflussung der Vertragsorgane untereinander und das Aufgreifen der Praxis durch andere internationale Akteure lässt sich Gewohnheitsrecht identifizieren. -- Dabei ist das menschenrechtliche Prinzip des Non-Refoulement besonders geeignet, dieses Potenzial der Vertragsorgane zu veranschaulichen. Hierbei handelt es sich um ein Rechtsprinzip, das zwar dem Grunde nach allgemein anerkannt ist, dessen Reichweite im Detail jedoch kontinuierlich umstritten ist. Erstmals wird ein umfassender Überblick über die gesamte Praxis der Vertragsorgane zum Prinzip des Non-Refoulement gegeben. Es wird gezeigt, wie sich die Vertragsorgane sowohl auf prozessualer Ebene als auch bei der Bestimmung des materiellen Schutzbereichs von Refoulementverboten einander annähern.
Terminology is a critical instrument for each researcher. Different terminologies for the same research object may arise in different research communities. By this inconsistency, many synergistic effects get lost. Theories and models will be more understandable and reusable if a common terminology is applied. This paper examines the terminological (in)consistence for the research field of job-shop scheduling by a literature review. There is an enormous variety in the choice of terms and mathematical notation for the same concept. The comparability, reusability and combinability of scheduling methods is unnecessarily hampered by the arbitrary use of homonyms and synonyms. The acceptance in the community of used variables and notation forms is shown by means of a compliance quotient. This is proven by the evaluation of 240 scientific publications on planning methods.
Competence development must change at all didactic levels to meet the new requirements triggered by digitization. Unlike classic learning theories and the resulting popular approaches (e.g., sender-receiver model), future-oriented vocational training must include new learning theory impulses in the discussion about competence acquisition. On the one hand, these impulses are often very well elaborated on the theoretical side, but the transfer into innovative learning environments - such as learning factories - is often still missing. On the other hand, actual learning factory (design) approaches often concentrate primarily on the technical side. Subject-oriented learning theory enables the design of competence development-oriented vocational training projectsin learning factories in which persons can obtain relevant competencies for digitization. At the same time, such learning theory approaches assume a potentially infinite number of learning interests and reasons. Following this, competence development is always located in an institutional or organizational context. The paper conceptionally answers how this theoryimmanent challenge is synthesizable with the reality of organizationally competence development requirements.
Handbuch der ERP-Auswahl
(2023)
The authors propose that while tacit knowledge is a valuable resource for developing new business models, its externalization presents several challenges. One major challenge is that individuals often don’t recognize their tacit knowledge resources, while another is the reluctance to share one’s knowledge with others. Addressing these challenges, the authors present an application-oriented serious game-based haptic modeling approach for externalize tacit knowledge, which can be used to develop the first versions of business models based on tacit knowledge. Both conceptual and practical design fundamentals are presented based on elaborated theoretical approaches, which were developed with the help of a design science approach. The development of the research process is presented step by step, whereby we focused on the high accessibility of the presented research. Practitioners are presented with guidelines for implementing their serious game projects. Scientists benefit from starting points for their research topics of externalization, internalization, and socialization of tacit knowledge, development of business models, and serious games or gamification. The paper concludes with open research desiderata and questions from the presented research process.
Mit RASSCO siedeln
(2023)
Die Jeckes-Forschung hat sie übersehen, die Architekturgeschichte hat ihre Bauten ignoriert, ihre Emigration stand unter dem Stigma des "Pakts mit dem Teufel": die nach 1933 aus dem nationalsozialistischen Deutschen Reich emigrierten Juden, die im Rahmen der "Kapitalisten-Alija" nach Palästina gelangten und dort in Mittelstandsdörfern angesiedelt wurden. Organisiert wurde ihre Emigration durch die Baugesellschaft RASSCO, die 1934 im Zuge des Haavara-Abkommens für den Siedlungstransfer gegründet wurde. Bis Kriegsausbruch wurden verschiedene Siedlungen realisiert, darunter Kfar Schmarjahu und die "Siedlung der Württemberger" Schawej Zion, bis zur Staatsgründung rund zwei Dutzend. Ines Sonder und Joachim Trezib dokumentieren erstmals den gesamten Prozess der Übersiedlung von der politischen Dimension des Haavara-Transfers bis hin zu den Einzelschicksalen der Siedlerfamilien aus Deutschland. Sie eröffnen somit eine neue Perspektive auf die Fünfte Alija - durch ihr reiches, bislang unveröffentlichtes Bildmaterial auch in visueller Hinsicht.
Many widely used observational data sets are comprised of several overlapping instrument records. While data inter-calibration techniques often yield continuous and reliable data for trend analysis, less attention is generally paid to maintaining higher-order statistics such as variance and autocorrelation. A growing body of work uses these metrics to quantify the stability or resilience of a system under study and potentially to anticipate an approaching critical transition in the system. Exploring the degree to which changes in resilience indicators such as the variance or autocorrelation can be attributed to non-stationary characteristics of the measurement process – rather than actual changes in the dynamical properties of the system – is important in this context. In this work we use both synthetic and empirical data to explore how changes in the noise structure of a data set are propagated into the commonly used resilience metrics lag-one autocorrelation and variance. We focus on examples from remotely sensed vegetation indicators such as vegetation optical depth and the normalized difference vegetation index from different satellite sources. We find that time series resulting from mixing signals from sensors with varied uncertainties and covering overlapping time spans can lead to biases in inferred resilience changes. These biases are typically more pronounced when resilience metrics are aggregated (for example, by land-cover type or region), whereas estimates for individual time series remain reliable at reasonable sensor signal-to-noise ratios. Our work provides guidelines for the treatment and aggregation of multi-instrument data in studies of critical transitions and resilience.
Development of self-concept and task interest has been shown to be affected by social comparison processes in a variety of cross-sectional studies. A potential explanation for these effects is an effect of social comparative performance feedback on an individual’s self-evaluation of performance, which in turn influences development of self-concept and task interest. There are, however, only few studies addressing this topic with experimental designs. This study was aimed at closing this research gap by experimentally manipulating social comparative performance. Feedback given was based on 2 × 2 experimental conditions: social position (high vs. low) and average performance of the reference group (high vs. low). Results show a strong effect of social position on self-evaluation of performance and smaller effects on self-concept and task interest.
The retention of actinides in different oxidation states (An(X), X = III, IV, VI) by a calcium-silicate-hydrate (C-S-H) phase with a Ca/Si (C/S) ratio of 0.8 was investigated in the presence of gluconate (GLU). The actinides considered were Am(III), Th(IV), Pu(IV), and U(VI). Eu(III) was investigated as chemical analogue for Am(III) and Cm(III). In addition to the ternary systems An(X)/GLU/C-S-H, also binary systems An(X)/C-S-H, GLU/C-S-H, and An(X)/GLU were studied. Complementary analytical techniques were applied to address the different specific aspects of the binary and ternary systems. Time-resolved laser-induced luminescence spectroscopy (TRLFS) was applied in combination with parallel factor analysis (PARAFAC) to identify retained species and to monitor species-selective sorption kinetics. ¹³C and ²⁹Si magic-angle-spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy and X-ray photoelectron spectroscopy (XPS) were applied to determine the bulk structure and the composition of the C-S-H surface, respectively, in the absence and presence of GLU. The interaction of Th(IV) with GLU in different electrolytes was studied by capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP-MS). The influence of GLU on An(X) retention was investigated for a large concentration range up to 10⁻² M. The results showed that GLU had little to no effect on the overall An(X) retention by C-S-H with C/S of 0.8, regardless of the oxidation state of the actinides. For Eu(III), the TRLFS investigations additionally implied the formation of a Eu(III)-bearing precipitate with dissolved constituents of the C-S-H phase, which becomes structurally altered by the presence of GLU. For U(VI) sorption on the C-S-H phase, only a small influence of GLU could be established in the luminescence spectroscopic investigations, and no precipitation of U(VI)-containing secondary phases could be identified.
Defining the metaverse
(2023)
The term Metaverse is emerging as a result of the late push by multinational technology conglomerates and a recent surge of interest in Web 3.0, Blockchain, NFT, and Cryptocurrencies. From a scientific point of view, there is no definite consensus on what the Metaverse will be like. This paper collects, analyzes, and synthesizes scientific definitions and the accompanying major characteristics of the Metaverse using the methodology of a Systematic Literature Review (SLR). Two revised definitions for the Metaverse are presented, both condensing the key attributes, where the first one is rather simplistic holistic describing “a three-dimensional online environment in which users represented by avatars interact with each other in virtual spaces decoupled from the real physical world”. In contrast, the second definition is specified in a more detailed manner in the paper and further discussed. These comprehensive definitions offer specialized and general scholars an application within and beyond the scientific context of the system science, information system science, computer science, and business informatics, by also introducing open research challenges. Furthermore, an outlook on the social, economic, and technical implications is given, and the preconditions that are necessary for a successful implementation are discussed.
Using time-resolved x-ray diffraction, we demonstrate the manipulation of the picosecond strain response of a metallic heterostructure consisting of a dysprosium (Dy) transducer and a niobium (Nb) detection layer by an external magnetic field. We utilize the first-order ferromagnetic–antiferromagnetic phase transition of the Dy layer, which provides an additional large contractive stress upon laser excitation compared to its zerofield response. This enhances the laser-induced contraction of the transducer and changes the shape of the picosecond strain pulses driven in Dy and detected within the buried Nb layer. Based on our experiment with rare-earth metals we discuss required properties for functional transducers, which may allow for novel field-control of the emitted picosecond strain pulses.
Mehr als nur Ehefrauen
(2023)
Frauen im Widerstand
(2023)
Krisen in Afganisthan
(2023)
The musculoskeletal system provides support and enables movement to the body, and its deterioration is a crucial aspect of age-related functional decline. Mesenchymal stromal cells (MSCs) play an important role in musculoskeletal homeostasis due to their broad differentiation potentials and their ability to support osteogenic and myogenic tissue maintenance and regeneration. In the bone, MSCs differentiate either into osteochondrogenic progenitors to form osteocytes and chondrocytes, or increasingly with age into adipogenic progenitors which give rise to bone-resident adipocytes. In skeletal muscle, during healthy regeneration MSCs provide regulatory signals that activate local, tissue-specific stem cells, known as satellite cells, which regenerate contractile myofibres. This process involves a significant cross-talk to immune cells stemming from both lymphoid and myeloid lineages. During ageing, muscle-resident MSCs undergo increased adipogenic lineage commitment, causing niche changes that contribute to fatty infiltration in muscles. These shifts in cell populations in bone lead to the loss of osteogenic cells and subsequently osteoporosis, or in muscle to impaired regeneration and to the development of sarcopenia. However, the signals that drive transition of MSCs into their respective cellular fates remain elusive.
This thesis aims to elucidate the transcriptional shifts modulating cell states and cell types in musculoskeletal MSC fate determination. Single-cell RNA-sequencing (scRNA-seq) was used to characterise cell type-specific transcript regulation. State-of-the-art bioinformatics tools were combined with different analytical platforms that include both droplet-based scRNA-seq for large heterogeneous populations, and microfluidics-based scRNA-seq to assess small, rare subpopulations. For each platform, distinct computational pipelines were established including filtering steps to exclude low-quality cells, and data visualisation was performed by dimensionality reduction. Downstream analysis included clustering, cell type annotation, and differential gene expression to investigate transcriptional states in defined cell types during ageing and injury in the muscle and bone. Finally, a novel tool to assess publication activities in defined areas of research for the identified marker genes was developed.
The results in the bone indicate that ageing MSCs increasingly commit towards an adipogenic fate at the expense of osteogenic specialisation. The data also suggests that significant cell population shifts of MSC-type fibro-adipogenic progenitors during muscle ageing underlie the pathologies observed in homeostatic and post-injury regenerative conditions. High-throughput visualisation of publication activity for candidate genes enabled more effective biological evaluation of scRNA-seq data. These results expose critical age-related changes in the stem cell niches of skeletal muscle and bone, highlight their respective sensitivity to nutrition and pathology, and elucidate novel factors that modulate stem cell-based regeneration. Targeting these processes might improve musculoskeletal health in the context of ageing and prevent the negative effects of pathological lineage determination.
Im Vergleich zu Umfragen an Wahrscheinlichkeitsstichproben bieten Umfragen an Access-Panels, die auf Nicht-Wahrscheinlichkeitsstichproben basieren, unbestreitbare wirtschaftliche Vorteile. Diese Vorteile gehen jedoch mit unvermeidbaren Qualitätseinbußen einher, die auch dann bestehen bleiben, wenn Erstere sehr niedrige Responseraten haben. Daher müssen die wirtschaftlichen Vorteile und die methodischen Einschränkungen gegeneinander abgewogen werden. Es wird argumentiert, dass diese Abwägung anhand normativer Festlegungen erfolgen muss. Unter Anwendung der hier vorgeschlagenen Maßstäbe kommt der Beitrag zu dem Schluss, dass die Qualitätsansprüche an über Massenmedien verbreitete Meinungsumfragen höher sein sollten als für rein (sozial)wissenschaftliche Zwecke.
A review of all research papers published in the European Sociological Review in 2016 and 2017 (N = 118) shows that only a minority of papers clearly define the parameter of interest and provide sufficient reasoning for the selected control variables of the statistical analysis. Thus, the vast majority of papers does not reach minimal standards for the selection of control variables. Consequently, a majority of papers interpret biased coefficients, or statistics without proper sociological meaning. We postulate that authors and reviewers should be more careful about control variable selection. We propose graphical causal models in the form of directed acyclic graphs as an example for a parsimonious and powerful means to that end.
Pichia pastoris (syn. Komagataella phaffi) is a distinguished expression system widely used in industrial production processes. Recent molecular research has focused on numerous approaches to increase recombinant protein yield in P. pastoris. For example, the design of expression vectors and synthetic genetic elements, gene copy number optimization, or co-expression of helper proteins
(transcription factors, chaperones, etc.). However, high clonal variability of transformants and low screening throughput have hampered significant success.
To enhance screening capacities, display-based methodologies inherit the potential for efficient isolation of producer clones via fluorescence-activated cell sorting (FACS). Therefore, this study focused on developing a novel clone selection method that is based on the non-covalent attachment of Fab fragments on the P. pastoris cell surface to be applicable for FACS.
Initially, a P. pastoris display system was developed, which is a prerequisite for the surface capture of secreted Fabs. A Design of Experiments approach was applied to analyze the influence of various genetic elements on antibody fragment display. The combined P. pastoris formaldehyde dehydrogenase promoter (PFLD1), Saccharomyces cerevisiae invertase 2 signal peptide (ScSUC2), - agglutinin (ScSAG1) anchor protein, and the ARS of Kluyveromyces lactis (panARS) conferred highest display levels.
Subsequently, eight single-chain variable fragments (scFv) specific for the constant part of the Fab heavy or light chain were individually displayed in P. pastoris. Among the tested scFvs, the anti-human CH1 IgG domain scFv allowed the most efficient Fab capture detected by flow cytometry.
Irrespective of the Fab sequence, exogenously added as well as simultaneously secreted Fabs were successfully captured on the cell surface. Furthermore, Fab secretion capacities were shown to correlate to the level of surface-bound Fabs as demonstrated for characterized producer clones.
Flow-sorted clones presenting high amounts of Fabs showed an increase in median Fab titers (factor of 21 to 49) compared to unsorted clones when screened in deep-well plates. For selected candidates, improved functional Fab yields of sorted cells vs. unsorted cells were confirmed in an upscaled shake flask production. Since the scFv capture matrix was encoded on an episomal plasmid with inherently unstable autonomously replicating sequences (ARS), efficient plasmid curing was observed after removing the selective pressure. Hence, sorted clones could be immediately used for production without the need to modify the expression host or vector. The resulting switchable display/secretion system provides a streamlined approach for the isolation of Fab producers and subsequent Fab production.
Neben kleineren, meist automatisierten Update-Aktivitäten zur Fehlerbehebung und zur Steigerung der Performance von ERP-Systemen, gibt es größere Updates mit umfangreicheren Aktualisierungen und Erweiterungen der Software – auch „Updateprojekte“ oder „Upgrades“ genannt. Ein ERP-Upgrade beschreibt einen größeren Änderungsprozess, der die Nutzung neuer Technologien ermöglicht und das System mit (neuen) Geschäftsstrategien in Einklang bringt. Upgrades tragen zur Verbesserung der Software bei und sind klar zu unterscheiden von geringfügigen Änderungen innerhalb einer Version eines ERP-Systems.
Unveiling the Local Universe
(2023)
Auffälliges Verhalten von Kindern und Jugendlichen im schulischen Alltag zeugt oftmals von den schwierigen Lebenssituationen, in denen sie aufwachsen. Für Lehr- und pädagogische Fachkräfte stellt der Umgang mit den daraus resultierenden Konflikten die größte Herausforderung dar. In diesem Buch werden Ansätze und Lösungen für das Verstehen und Handeln in schwierigen pädagogischen Situationen in der Schule vorgestellt. Außerdem bietet ein inklusiver Förderansatz Impulse für die Schulentwicklung zur Prävention auffälligen Verhaltens.
Dotting the “I think”
(2023)
This chapter discusses a central problem in Sebastian Rödl’s Self-Consciousness and Objectivity and in Wittgenstein’s Tractatus. In a statement of the form “I think p”, the words “I think” do not contribute to the content, and yet they are not redundant. In other words, a thinking subject is not something and yet not nothing. But then in what sense is a thinking subject a part of the world? The problem is intractable on a merely negative understanding of “I think”, like Anscombe’s merely negative thesis, endorsed by Rödl, that “I” is not a referring expression. In search of a positive understanding, this chapter proposes to understand “I think” by comparison to “hello”. A speaking subject is the expression of mutual presence in conversation – in that sense a limit of the world. Such expression may be compared to facial expression, with the crucial difference that a verbal expression can be taken up – i.e., repeated – in the third person. A speaking subject, then, is potentially absent from conversation, and in that sense a part of the world.
Fördern und Zensieren
(2023)
Deutsche Blauhelme in Afrika
(2023)
Warum trat die Bundesregierung ab Ende der 1980er Jahre weltweit als sicherheitspolitischer Akteur in Erscheinung? Warum engagierte sie sich in multinationalen Missionen der Vereinten Nationen etwa in Somalia, Namibia und Ruanda mit Soldaten oder Polizisten - und in anderen Missionen in Afrika im gleichen Zeitraum nicht? Gestützt auf ministerielle Archivquellen untersucht Torsten Konopka die Prozesse der nationalen Entscheidungsfindung, die zu einer Beteiligung oder Nichtbeteiligung an VN-Missionen in Afrika führten. Das Buch leistet einen politik- sowie militärgeschichtlichen Beitrag zur Genese der frühen Auslandsverwendungen der Bundeswehr.
Umfassend oder überfrachtet?
(2023)
In der Theorie klingt es erst mal pädagogisch und didaktisch verlockend: Umfassend ausgebildete Lehrkräfte verharren nicht stur in ihren fachlichen Grenzen, sondern unterrichten Phänomene in ihren mannigfaltigen Zusammenhängen. So erwerben Schüler*innen die Möglichkeit, Sachverhalte umfassend aus verschiedenen Perspektiven zu betrachten und ihnen kompetent zu begegnen. Im Hinblick auf eine vollgestopfte Stundentafel scheint dies auch zeitlich effizient: Warum verschiedene Fächer aufwenden, wenn man drei oder vier Bildungsanliegen in einem zweistündigen Fach unterbringen kann?
Virtual Reality
(2023)
Social Media
(2023)
Wirtschaft-Arbeit-Technik
(2023)
Lernangebote im Fach Wirtschaft-Arbeit-Technik tragen dazu bei, die Persönlichkeit der Schülerinnen und Schüler zu stärken und Handlungskompetenzen zu erwerben mit dem Ziel, dass sie gegenwärtige und zukünftige Lebensaufgaben im privaten und öffentlichen Bereich sowie in der Berufs- und Arbeitswelt zunehmend als mündige und selbstbestimmte Bürgerinnen und Bürger aktiv bewältigen können. Der Band stellt die fachdidaktischen Grundlagen des Unterrichts im Fach Wirtschaft-Arbeit-Technik dar. Behandelt werden fachdidaktische Prinzipien, fachbezogene Aspekte zur Leistungsbeurteilung und -bewertung, der Einsatz von Medien sowie Praxiskontakte und außerschulische Lernorte.