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Dendritic cells (DCs) are the most potent antigen-presenting cells of the immune system and are currently being investigated in clinical applications as cancer vaccines. An efficient cryopreservation method would greatly contribute to their use in clinical trials. We have established a method for freezing of DCs derived from peripheral blood mononuclear cells using the plasma expander Gelifundol. This enabled us to reduce the concentration of the toxic DMSO to 5%. The method could be performed without the addition of fetal calf serum or any other serum. After freezing, the viability of the DCs was 90%. The cells exhibited all the phenotypic characteristics (CD11c+, HLA-DR+, CD80+, CD83+, CD86+) of DCs, as tested by flow cytometry. Cells transfected with cDNA for the tumour antigen mucin expressed this protein on their surfaces in the same manner as before freezing. The stimulating capacity of a mixed lymphocyte culture was also preserved. These findings offer an efficient method for the cryopreservation of DCs for use in clinical trials.
Zu einigen morphosyntaktischen Assimilationsprozessen bei russischen Entlehnungen im Deutschen
(2001)
Einleitung
(2001)
Dopamine-induced epithelial K+ and Na+ movements in the salivary ducts of Periplaneta americana
(2001)
Sprechalter
(2001)
Andreas von Bülow
(2001)
International trade and spatial markets : trade policy from a theory of spatial pricing perspective
(2001)
Noll, V., Das brasiliansiche Portugiesisch : Herausbildung und Kontraste; Heidelberg, Winter, 1999
(2001)
Die wissenschaftliche Beschreibung des modernen Portugiesisch in der Grammatik von Eberhard Gaertner
(2001)
This paper presents four color intermediate-band photometry of the cluster A2218 carried out using the Wise Observatory 1 m telescope. A2218 is one of the richest clusters in the Abell catalogue, with richness class 4. We find that A2218 has an unusually low fraction of blue galaxies for its redshift (z=0.171), and is populated mainly by E/S0 galaxies, while starburst systems are very scarce. The few starbursting galaxies present populate the faint end of the cluster luminosity function, close to the limiting magnitude of our observations. The difference between A2218 and other clusters at similar redshifts is very remarkable, underlining the influence of cluster richness on the evolution of its member galaxies.
The psycho-social development of both preterm and term children (n=347) whose mothers reported tocolytic treatment was assessed at the ages of 2, 4.5, 8 years. Term children exposed to tocolysis showed a higher rate of psychiatric disorders as well as poorer cognitive and motor performance than controls. In the preterm children no adverse impact of tocolysis could be found. The results are discussed concerning possible ways in which tocolytic treatment may influence child development. Restrictions because of the preliminary character of this study and the need of further prospective studies to clarify the developmental impact of tocolysis are also considered.
Proof of Chirality of J-Aggregates Spontaneously and Enantioselectivily Generated from Achiral Dyes
(2001)
First, we review the current status of the detection of strong `external' variability in the CLASS gravitational B1600+434, focusing on the 1998 VLA 8.5-GHz and 1998/9 WSRT multi-frequency observations. We show that this data can best be explained in terms of radio-microlensing. We then proceed to show some preliminary results from our new multi-frequency VLA monitoring program, in particular the detection of a strong feature (~30%) in the light curve of the lensed image which passes predominantly through the dark-matter halo of the lens galaxy. We tentatively interpret this event, which lasted for several weeks, as a radio-microlensing caustic crossing, i.e. the superluminal motion of a micro-arcsec-scale jet-component in the lensed source over a single caustic in the magnification pattern, that has been created by massive compact objects along the line-of-sight to the lensed image.
We describe automatic procedures for the selection of DA white dwarfs in the Hamburg/ESO objective-prism survey (HES). For this purpose, and the selection of other stellar objects (e.g., metal-poor stars and carbon stars), a flexible, robust algorithm for detection of stellar absorption and emission lines in the digital spectra of the HES was developed. Broad band (U-B, B-V) and intermediate band (Strömgren c_1) colours can be derived directly from HES spectra, with precisions of sigma U-B=0.092 mag; sigma B-V=0.095 mag; sigma c_1=0.15 mag. We describe simulation techniques that allow one to convert model or slit spectra to HES spectra. These simulated objective-prism spectra are used to determine quantitative selection criteria, and for the study of selection functions. We present an atlas of simulated HES spectra of DA and DB white dwarfs. Our current selection algorithm is tuned to yield maximum efficiency of the candidate sample (minimum contamination with non-DAs). DA candidates are selected in the B-V versus U-B and c_1 versus W_lambda (Hbeta +Hgamma +Hdelta ) parameter spaces. The contamination of the resulting sample with hot subdwarfs is expected to be as low as ~ 8%, while there is essentially no contamination with main sequence or horizontal branch stars. We estimate that with the present set of criteria, ~ 80% of DAs present in the HES database are recovered. A yet higher degree of internal completeness could be reached at the expense of higher contamination. However, the external completeness is limited by additional losses caused by proper motion effects and the epoch differences between direct and spectral plates used in the HES. Based on observations collected at the European Southern Observatory, La Silla and Paranal, Chile.
We report on deep multi-color imaging (R5sigma = 26) of the Chandra Deep Field South, obtained with the Wide Field Imager (WFI) at the MPG/ESO 2.2 m telescope on La Silla as part of the multi-color survey COMBO-17. As a result we present a catalogue of 63 501 objects in a field measuring 31farcm5 x 30arcmin with astrometry and BVR photometry. A sample of 37 variable objects is selected from two-epoch photometry. We try to give interpretations based on color and variation amplitude.
Cosmological microlensing
(2001)
Quasar Microlensing
(2001)
Lese-Rechtschreibschwäche
(2001)
Laser beam melt ablation - a contact-free machining process - offers several advantages compared to conventional processing mechanisms: there exists no tool wear and even extremely hard or brittle materials can be processed. During ablation the workpiece is molten by a CO2-laser beam, this melt is then driven out by the impulse of a process gas. The idea behind laser ablation is rather simple, but it has a major limitation in practical applications: with increasing ablation rates surface quality of the workpiece processed declines rapidly. At high ablation rates, depending on the process parameters different periodic-like structures can be observed on the ablated surface. These structures show a dependence on the line energy, which has been identified as a fundamental control parameter. In dependence on this parameter several regimes with different behaviours of the process have been separated. These regimes are distinguishable as well in the surfaces obtained as in the signals gained by the measurement of the process emissions. Further aim is to identify the different modes of the system and reach a deeper understanding of the dynamics of the molten material in order to understand the formation of these surface structures. With this it should be possible to influence the system in the direction of avoiding structure formation even at high ablation rates. Relying on the results on-line monitoring and control of the process should be studied.
We analyse the X-ray light curves of compact objects using linear and nonlinear time series analysis methods. A Power Density Spectrum (PDS) describes the overall second order properties of the observed data well. To look beyond we propose the nonlinear Q-statistic to detect an asymmetry of the time series. This allows us to find relevant time scales. This method even grants a subclassification of the known states of X-ray sources.
Potsdamer UNO-Konferenzen
(2001)
Vorwort
(2001)
Schleiermachers Kritik an der Aufklärung und Aufklärungspädagogik : eine Überwindung der Aufklärung?
(2001)
Lernprozesse steuern
(2001)
"Selbstmanagement" versus "Staatsamateur" : Spitzensportkarrieren in Deutschland und Frankreich
(2001)
Bildungsziel Europa
(2001)
Die Informatik durchdringt zusehends fast alle Bereiche der Ausbildung und der Berufswelt. Die Stichwörter berücksichtigen die Geschichte der Informatik und den heutigen Stand ihrer Methoden und beziehen aktuelle und absehbare Entwicklungen mit ein. Das ausführliche Register sorgt dafür, dass man den gewünschten Begriff sofort im Blick hat. Rund 3 000 Stichwörter, etwa 700 Abbildungen, Register.
Ageing and executive control
(2001)