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A package of five FORTRAN programs that provides for fast user-controlled analyses of reading eye fixations is described. The package requires the data to be in a fixation format and to be rescaled to screen dimensions. OLDEYE identifies six types of fixations and calculates descriptive statistics on each of them, on their associated saccades, and on their average pupil diameter. CONVRT represents the text as a string of words that can be coded according to experimentally relevant variables. PLTFIX prints fixation durations by letter position and sequence of occurrence. MODDAT is an interactive program for marking parts of the text in which the data quality is below acceptable standards. It also allows the correction of systematic errors due to calibration or drift. MATCH combines the outputs from OLDEYE, CONVRT, and MODDAT and calculates 11 dependent measures for every word. The output of MATCH is suitable for input to conventional multivariate statistical programs.
The present paper presents FORTRAN programs for reducing eye monitor output to fixations and for mapping these fixations to locations in the stimulus space. Flexible parameters of the fixations program allow for determination of the beginning and end of fixations under different resolution criteria and for indicating loss of accurate measurement. The calibration program is based on a rectangular 9-point fixation grid. Each fixation is rescaled within this grid by solving for a quadratic equation. The rescaled values are output in a flexibly determined rectangular coordinate system that is related to the stimulus space, such as character position on the screen. The programs were developed for the 60-Hz Applied Sciences corneal reflection eye monitor, but they may be used with a number of other systems.
[1-14C]-N-Acetyldopamine (NADA) was oxidized in the presence of methyl [3-3H]-β-alanate with mushroom tyrosinase. The complex mixture of reaction products was partly resolved by chromatographic procedures and analyzed by spectroscopic methods. Methyl-β-alanate is incorporated to only a small extent into oxidation products of NADA which inter alia are presumed to be oligomeric hydroxyquinones. After oxidation of [1-14C, 2-3H]-NADA with preparations from tanning Manduca sexta pupal cuticle, N-acetylnoradrenalin was identified as one of the products. Binding of radioactivity to melanin-like material was also observed. These results suggest that oxidation products different from those formulated usually for the crosslinkages between protein amino groups and N-acetyldopaquinone are deposited in darkly brown coloured insect cuticles during sclerotization.