Refine
Has Fulltext
- no (21)
Year of publication
Document Type
- Article (21) (remove)
Language
- English (21)
Is part of the Bibliography
- yes (21)
Keywords
- leucine zipper (2)
- protein engineering (2)
- AF10 (1)
- Basic helix-loop-helix leucine zipper (1)
- Cohesive ends (1)
- Coiled coils (1)
- DNA cleavage (1)
- Escherichia coli (1)
- GBSA (1)
- Genetic vectors (1)
Institute
Light switching of the activity of a coiled-coil protein, the AP-1 transcription factor, in living cells was made possible by the introduction of a designed azobenzene-cross-linked dominant negative peptide, XAFosW (red and yellow in the picture). In the dark, XAFosW showed decreased helical content and decreased affinity for target Jun proteins (green); irradiation at 365 nm enhanced helicity and target affinity.