Refine
Year of publication
- 2020 (250) (remove)
Document Type
- Article (158)
- Postprint (52)
- Doctoral Thesis (32)
- Other (4)
- Review (3)
- Habilitation Thesis (1)
Language
- English (250)
Keywords
- diversity (7)
- alien species (6)
- animal personality (6)
- coexistence (6)
- climate change (5)
- evolution (5)
- phytoplankton (5)
- Dictyostelium (4)
- ancient DNA (4)
- crystal-structure (4)
- dynamics (4)
- functional diversity (4)
- gene-expression (4)
- home range (4)
- traits (4)
- DNA methylation (3)
- Evolution (3)
- Genomics (3)
- NE81 (3)
- animal movement (3)
- carbohydrates (3)
- community ecology (3)
- competition (3)
- defense (3)
- epigenetics (3)
- functional traits (3)
- lake (3)
- lamin (3)
- light intensity (irradiance) (3)
- light variability (3)
- machine learning (3)
- metabolism (3)
- natal dispersal (3)
- nuclear envelope (3)
- nuclear lamina (3)
- nutrient supply (3)
- photosynthetic rate (3)
- phytoplankton communities (3)
- pigment composition (3)
- resource competition (3)
- root traits (3)
- simulation (3)
- specific root length (3)
- sucrose (3)
- thaliana (3)
- Achlya (2)
- Achlyaceae (2)
- Aluize (2)
- Aphid host (2)
- Aphidius ervi (2)
- Arabidopsis thaliana (2)
- Arabidopsis thaliana (arabidopsis) (2)
- BEEHAVE (2)
- Black Queen Hypothesis (2)
- CPG Islands (2)
- Carnivorous plant (2)
- Changane (2)
- Chemosensory genes (2)
- Clethra arborea (2)
- Costa Rica (2)
- D statistics (2)
- DNA methylation loss (2)
- DNA modification (2)
- EHV-1 (2)
- EWPC2019 (2)
- Ecology (2)
- Formaldehyde assimilation (2)
- GC content (2)
- Genome assembly (2)
- Genomik (2)
- Germany (2)
- Growth selection (2)
- Holocene environmental history (2)
- LPS (2)
- Larix (2)
- Late Quaternary vegetation (2)
- Lysiphlebus fabarum (2)
- MED16 (2)
- Martini force-field (2)
- Metschnikowia (2)
- Microcystis aeruginosa (2)
- Mitochondria (2)
- Museum collections (2)
- NMR spectroscopy (2)
- Northern Asia (2)
- OMICs tools (2)
- Orthologous Matrix (OMA) Project (2)
- Oyster Crassostrea-gigas (2)
- Paleogenetics (2)
- Parasitoid wasp (2)
- Pathway design (2)
- Phylogenetic analysis (2)
- Phylogenetics (2)
- Phylogenomics (2)
- Promiscuous enzymes (2)
- RNA interference (2)
- RNA virus (2)
- Raphidiopsis (2)
- Roridula gorgonias (2)
- Salmonella (2)
- Saprolegnia (2)
- Saprolegniaceae (2)
- Sequence alignment (2)
- Serine cycle (2)
- Shotgun sequencing (2)
- Siberia (2)
- Solidago canadensis (2)
- Solidago gigantea (2)
- Toll and Imd pathways (2)
- Transcriptome assembly (2)
- UV radiation (2)
- Urbanization (2)
- Venom proteins (2)
- Verkhoyansk mountains (2)
- Wind (2)
- abiotic stress (2)
- activation (2)
- agro-infiltration (2)
- alkylphospholipids (2)
- allometry (2)
- alphaherpesvirus (2)
- analytics (2)
- anthropogenic food subsidies (2)
- apis mellifera (2)
- apodemus-agrarius (2)
- aquatic ecosystems (2)
- aquatic fungi (2)
- aqueous-solution (2)
- area-based conservation (2)
- arthropod (2)
- artificial intelligence (2)
- assembly processes (2)
- autocorrelation (2)
- auxotrophy (2)
- avian personalities (2)
- axillary bud (2)
- biodiversity (2)
- biodiversity facets (2)
- bioinformatics (2)
- biological invasions (2)
- biological soil crusts (2)
- biomass (2)
- biotic filtering (2)
- biotin sulfoxide reductase (2)
- branching (2)
- cancer (2)
- carbon cycling (2)
- carrion ecology (2)
- cells (2)
- chloroplast (2)
- chromatin (2)
- chromatin regulation (2)
- circadian clock (2)
- climate dynamics (2)
- co-limitation (2)
- coefficient (2)
- colony viability (2)
- common‐garden experiment (2)
- concepts (2)
- conservation (2)
- constraint-based modeling (2)
- consumptive resistance (2)
- converting factor (2)
- cori cycle (2)
- crop diversity (2)
- cropping system (2)
- cryptic species complex (2)
- cyanobacteria (2)
- cyanobacterial sucrose-phosphatase (2)
- cytokines (2)
- dark virus (2)
- de novo genome assembly (2)
- decline (2)
- deep-sea (2)
- dispersal filtering (2)
- drought (2)
- droughts (2)
- dual GLP-1/glucagon receptor agonist (2)
- eastern continental Asia (2)
- ecological novelty (2)
- ecological speciation (2)
- ecosystem services provisioning (2)
- ecosystems (2)
- efficient (2)
- electric fish (2)
- endotoxin (2)
- enrichment experiments (2)
- environmental filtering (2)
- epigenetic variation (2)
- error reduction (2)
- evolutionary (2)
- evolutionary ecology (2)
- exendin-4 (2)
- exploratory-behavior (2)
- expression patterns (2)
- extinction drivers (2)
- feeding behaviour (2)
- fitness (2)
- fitness consequences (2)
- floating mat (2)
- flooded grasslands (2)
- fluorescence correlation spectroscopy (2)
- fluorescence microscopy (2)
- forage availability (2)
- forage gaps (2)
- formate dehydrogenase (2)
- freshwater (2)
- freshwater heterotrophic bacteria (2)
- functional richness (2)
- gene delivery (2)
- gene expression (2)
- generalized dissimilarity modelling (2)
- genetic differentiation (2)
- genetic diversity (2)
- genotype (2)
- geographic distribution (2)
- glacial maximum (2)
- glycogen (2)
- heat stress (2)
- heat stress memory (2)
- hierarchy-of-hypotheses approach (2)
- honey bees (2)
- hsp70 (2)
- human endotoxemia (2)
- hyperthermia (2)
- immunogenicity (2)
- in-vitro-synthesis (2)
- individual variation (2)
- infiltration (2)
- inflammation (2)
- inhibition (2)
- invasibility (2)
- invasion success (2)
- island disharmony (2)
- island syndromes (2)
- jasmonate (2)
- kernel density estimation (2)
- lactate (2)
- lake periphyton (2)
- landscape generator (2)
- landscape of fear (2)
- late pleistocene (2)
- light scattering (2)
- lipidation (2)
- lipids (2)
- lipid–lipid interactions (2)
- lipoplexes (2)
- maintenance (2)
- management (2)
- mapping (2)
- mechanisms (2)
- mediated delivery (2)
- membrane biophysics (2)
- membrane microdomains (2)
- meta-analysis (2)
- metabolomic (2)
- metabolomics (2)
- metagenome (2)
- microbial interactions (2)
- microcystin (2)
- microsatellites (2)
- migration (2)
- modeling (2)
- molecular architecture (2)
- molecular dynamics simulations (2)
- molecular phylogeny (2)
- molybdenum (2)
- molybdopterin synthase (2)
- movement ecology (2)
- multi‐ year flooding cycle (2)
- n-oxide reductase (2)
- natural-selection (2)
- nature conservation (2)
- necrobiome (2)
- network (2)
- nitrogen limitation (2)
- novel ecosystems (2)
- novel species (2)
- nucleus (2)
- one-carbon metabolism (2)
- osmotic-pressure (2)
- overhunting (2)
- pacific oyster (2)
- palaeogenomics (2)
- paleoenvironmental records (2)
- parameters (2)
- pathways (2)
- periplasmic nitrate reductase (2)
- personality-traits (2)
- phenotypic phase plane (2)
- phosphatidylserine (2)
- phosphorylation (2)
- phylogenetic diversity (2)
- plankton (2)
- plant Mediator (2)
- plant clonality (2)
- plant macrofossil data (2)
- plant naturalization (2)
- plant–soil feedback (2)
- plasma membrane (2)
- polarization (2)
- polymer degradation (2)
- potential-functions (2)
- priming (2)
- protein-carbohydrate interactions (2)
- protein-protein interaction (2)
- proteins (2)
- purifying selection (2)
- pyruvate (2)
- quorum sensing (2)
- random forest (2)
- range size (2)
- redfield ratio (2)
- regulate gene expression (2)
- reserve design (2)
- resilience (2)
- reveals (2)
- rhodobacter-capsulatus (2)
- rodents (2)
- root morphology (2)
- rural populations (2)
- salicylic-acid (2)
- salt stress (2)
- scaling (2)
- sedimentary ancient DNA (2)
- seed bank (2)
- self-assembly (2)
- sequencing error (2)
- serine cycle (2)
- shock proteins (2)
- shrub-encroachment (2)
- signal transduction (2)
- sirna transfection (2)
- soil texture (2)
- source regions (2)
- spontaneous reaction (2)
- standard metrics (2)
- starch (2)
- stress (2)
- stress memory (2)
- subcellular localization (2)
- sugar signalling (2)
- support vector machine (2)
- surface plasmon resonance (2)
- synthesis (2)
- systematic review (2)
- taxonomic (2)
- temporary wetland (2)
- testis (2)
- therapeutics (2)
- thermal-stress (2)
- transcript (2)
- transcription (2)
- transcriptional memory (2)
- transient expression (2)
- transition (2)
- trehalose 6-phosphate (2)
- trehalose 6‐ phosphate (Tre6P) (2)
- trophic apparatus (2)
- trypanosoma (2)
- vascular plants (2)
- vegetative growth (2)
- vernalization (2)
- vertebrate scavenger (2)
- warburg effect (2)
- wildlife management (2)
- xanthine dehydrogenase (2)
- zebularine (2)
- (SEPE) factors (1)
- 2Fe-2S cluster (1)
- ABCE model (1)
- AC electrokinetics (1)
- AC electrophoresis (1)
- AIDS (1)
- ALAN (1)
- AMP (1)
- APEC (1)
- APETALA2 (1)
- ATPS (1)
- Aktinzytoskelett (1)
- Altitude (1)
- Anreicherungsmethoden (1)
- Anthropometric measurement (1)
- Aptamer (1)
- Aptamers (1)
- Arabidopsis (1)
- Arctic (1)
- Argentina (1)
- Arktis (1)
- Artificial light at night (1)
- Bacillus (1)
- Bacteria (1)
- Bakterien (1)
- Bandung District (1)
- Bestandsparameter (1)
- Bildanalyse (1)
- Biochemie (1)
- Biogeographie (1)
- Bivalve (1)
- Blunt trauma (1)
- Botulinum neurotoxin (1)
- Brachionus (1)
- Brachionus calyciflorus (1)
- C stocks (1)
- CLE7 (1)
- CO2 degassing (1)
- CO2 flux (1)
- CO2-Entgasung (1)
- CPR5 (1)
- Campylomormyrus (1)
- Canopy parameters (1)
- Cetaceans (1)
- Chemotaxis (1)
- Chloroplast gene expression (1)
- Chloroplasten-Genexpression (1)
- Climate (1)
- Climate change (1)
- Coiled Coil (1)
- Common noctule bat (1)
- Cyanobacteria (1)
- Cyanobakterien (1)
- DRYM (1)
- Daptomycin (1)
- Debris (1)
- Delta F508 mutation (1)
- Development (1)
- Diffusion (1)
- Drought tolerance (1)
- E. coli (1)
- EMS (1)
- Early Starvation 1 (1)
- Early starvation protein (1)
- Ecotoxicology (1)
- Einschätzung der Diffusion (1)
- Einzelmolekülkraftspektroskopie (1)
- Einzelzellbewegung (1)
- Electric (1)
- Electric organ discharge (1)
- Electrocyte features (1)
- Emulsionen (1)
- Environmental gradients (1)
- Equus dalianensis (1)
- Equus przewalskii (1)
- Erdbeben (1)
- Escherichia coli (1)
- European brown (1)
- European lamprey (1)
- Eutrophierung (1)
- Exposure (1)
- F1-hybrids (1)
- F2-hybrids (1)
- FRAP (1)
- Fe-S cluster assembly (1)
- Feldmaus (1)
- Fibres (1)
- Flagella (1)
- Flagellen (1)
- Förster Resonanz Energie Transfer (FRET) (1)
- Förster resonance energy transfer (FRET) (1)
- GPI (1)
- GPS tracking (1)
- Gaussian process (1)
- Genomic Mining (1)
- Glucan water dikinase (1)
- Glucan-Wasser-Dikinase (1)
- Grasland (1)
- Griffithsin (1)
- GxE interaction (1)
- HIREC (1)
- HIV (1)
- HMA sponge (1)
- HPLC (1)
- HSFA2 (1)
- HSP90.1 (1)
- Habitat fragmentation (1)
- Habitat use (1)
- Herzentwicklung (1)
- Herzklappe (1)
- High resolution (1)
- Histidin-Metall Koordination (1)
- Histone deacetylase inhibitor (1)
- Hochdurchsatzsequenzierung (1)
- Home range (1)
- Hydrogel (1)
- Hymenolepis (1)
- Höhe (1)
- IR laser (1)
- ISC (1)
- Indonesian National Growth Reference Charts (1)
- Influenza-A (1)
- Inhibitory cytokines (1)
- Interdigitated electrodes (1)
- Interleukin-35 (1)
- Interspecific aggression (1)
- Japan (1)
- Karube (1)
- Klima (1)
- Kohlenhydrate (1)
- Lampetra (1)
- Land use (1)
- Lepus europaeus (1)
- Like-Early Starvation 1 (1)
- Like-Early starvation protein (1)
- Litter (1)
- Local adaptation (1)
- Lynx lynx (1)
- Mechanosensation (1)
- Membran (1)
- Metabolic Engineering (1)
- Methane (1)
- Methanogene (1)
- Methanotrophe (1)
- Microbial communities (1)
- Microtus arvalis (1)
- Mikrobielle Gemeinschaften (1)
- Mikrogel (1)
- Mikroviskosität (1)
- Minimum convex polygons (MCPs) (1)
- Mitogenome (1)
- Moco biosynthesis (1)
- Molecular dielectrophoresis (1)
- Molekular-dynamik (1)
- Mortality (1)
- Movement (1)
- MutMap (1)
- Myodus glareolus (1)
- NBR1 (1)
- NIF (1)
- Natural Products (1)
- Naturschutz (1)
- Naturstoffe (1)
- Netzwerke (1)
- Nicotiana tabacum (1)
- Norway (1)
- Nostoc punctiforme (1)
- O-antigen (1)
- Ordnung der Partikel auf der Oberfläche (1)
- Organic dyes (1)
- PNIPAM (1)
- POLS (1)
- PUFA composition (1)
- Pantoea stewartii (1)
- Permafrostdegradation (1)
- Pflanzen (1)
- Pflanzenzellen (1)
- Phosphoglucan water dikinase (1)
- Phosphoglucan-Wasser-Dikinase (1)
- Phosphorylation process (1)
- Phosphorylierungsprozess (1)
- Photorespiration (1)
- Phragmites australis (1)
- Plastic (1)
- Pleistocene caballine horses (1)
- Pollution (1)
- Polyelektrolyt-Multischichten (1)
- Population viability analysis (1)
- Populationsgenetik (1)
- Porpicide (1)
- Preference (1)
- Primula (1)
- Protein (1)
- Protein complex assembly (1)
- Proteinkomplexassemblierung (1)
- ROF1 (1)
- ROS (1)
- Redox (1)
- Rheologie (1)
- Ribosome profiling (1)
- Rötelmaus (1)
- SAHA (1)
- SRAssembler (1)
- SUF (1)
- SUMO (1)
- Secondary Metabolites (1)
- Sekundärmetabolite (1)
- Shellfish (1)
- Siberian arctic (1)
- Signalübertragung (1)
- Simulation (1)
- Single-cell motility (1)
- Stage-based model (1)
- Starch metabolism (1)
- Stranding (1)
- Stärkestoffwechsel (1)
- Submarine permafrost (1)
- Submariner Permafrost (1)
- Subsea permafrost (1)
- Synthetic Biology (1)
- TSD (1)
- Translation (1)
- Translation feedback regulation (1)
- Translationsfeedbackregulation (1)
- Trockentoleranz (1)
- Ulcerative colitis (1)
- Urban ecology (1)
- Verteilungsmuster (1)
- Wasser-in-Wasser (1)
- Weakly electric fish (1)
- Wirkstoff-Freisetzun (1)
- World Health Organization Child Growth Standards (1)
- Xaa-Pro aminopeptidase (1)
- Xanthomonas (1)
- Xpm (1)
- Zahnwale (1)
- Zebrafisch (1)
- Zellform (1)
- actin cytoskeleton machine (1)
- actuation (1)
- adaptation (1)
- advection-diffusion (1)
- agent-based modelling (1)
- aggregation (1)
- agriculture (1)
- air pollutant (1)
- aldehyde oxidase (AOX) (1)
- amination (1)
- amperometry (1)
- anti bacterial (1)
- antimicrobial defense (1)
- antimicrobial peptide (1)
- antiviral agent (1)
- arabidopsis thaliana (1)
- area (1)
- artificial neural network (1)
- assessment (1)
- assessment of the diffusion (1)
- atomic force microscopy (1)
- avoidance rate (1)
- bacteria (1)
- bacterial (1)
- bacterial outer membrane vesicles (1)
- bacterial symbionts (1)
- bacteriocytes (1)
- bacteriophage (1)
- behavioral syndromes (1)
- behaviour (1)
- behavioural adjustment (1)
- behavioural flexibility (1)
- behavioural syndrome (1)
- belowground herbivory (1)
- biochemistry (1)
- biofilm (1)
- biofilm formation (1)
- biogeography (1)
- biomaterials (1)
- biosensor (1)
- biosensors (1)
- birth weight (1)
- body height (1)
- bone accumulation (1)
- breastfed infant development (1)
- breath (1)
- campylomormyrus (1)
- carbon (1)
- carbon nanotubes (1)
- cardiac valves (1)
- cassava bacterial blight (1)
- catalysis (1)
- catch-up growth (1)
- cell death (1)
- cell fixation (1)
- cell morphogenesis (1)
- cell shape (1)
- cell-based in vitro assay (1)
- cells epidermis (1)
- cervical spine (1)
- children (1)
- chloroplast genome (1)
- climate mitigation (1)
- clonal growth (1)
- close packing (1)
- coagulans (1)
- coarse grained Molekulardynamiken (1)
- coarse grained molecular dynamics (1)
- coarse-graining (1)
- coiled coil (1)
- competitive exclusion (1)
- constrains (1)
- costs (1)
- crop yield failure (1)
- cyclops vicinus (1)
- cystic fibrosis (1)
- cytoskeleton (1)
- decisions (1)
- deep biosphere (1)
- degree of urbanization (1)
- density (1)
- development goals (1)
- developmental constraints (1)
- diatoms (1)
- dichteste Packung (1)
- dietary patterns (1)
- diffusion (1)
- direct electron (1)
- disease resistance (1)
- dispersal (1)
- distribution pattern (1)
- divergence time (1)
- double flowers (1)
- drug metabolism (1)
- drug release (1)
- dryland grasslands (1)
- early life trauma (1)
- earthquake (1)
- eco-evolutionary feedbacks (1)
- ecological modelling (1)
- ecosystem functioning (1)
- ecosystem services (1)
- elbow breadth (1)
- electric organ discharge (1)
- electrochemical biosensors (1)
- electrocyte ontogeny (1)
- electrocyte ultrastructure (1)
- electropolymerization (1)
- embryo development (1)
- emergent facilitation (1)
- emission inventory (1)
- emission modeling (1)
- emulsion (1)
- endoreduplication (1)
- energy metabolism (1)
- enrichments methods (1)
- environmental change (1)
- environmental effects (1)
- environmental impact (1)
- enzyme catalytic rates (1)
- enzyme evolution (1)
- epithionitrile (1)
- eurasian lynx (1)
- eutrophication (1)
- exopolysaccharide (1)
- exploitation (1)
- expression profile (1)
- extreme events (1)
- extreme weather event (1)
- extremotolerance (1)
- fasciation (1)
- fatty acids (1)
- fertility (1)
- final body height (1)
- flavin adenine dinucleotide (FAD) (1)
- flavoprotein (1)
- flight height (1)
- flower development (1)
- food quality (1)
- foodweb (1)
- foraging (1)
- formaldehyde (1)
- formate assimilation (1)
- formate dehydrogenases (1)
- fragmented landscapes (1)
- free-living (1)
- functional complementation (1)
- functional response (1)
- gate effect (1)
- gene duplication (1)
- gene expression profiles (1)
- gene regulatory networks (1)
- genome assembly (1)
- genome-scale metabolic (1)
- genomic prediction (1)
- genomic selection (1)
- genomics (1)
- gestational weight gain (1)
- giving-up density (1)
- glacial lakes (1)
- glucosinolate hydrolysis (1)
- gold (1)
- gradient boosting (1)
- grassland (1)
- grazing (1)
- grobkörnig (1)
- growth (1)
- growth rate (1)
- habitat loss (1)
- hare (1)
- hatchery (1)
- hatching success (1)
- heart development (1)
- height (1)
- heme (1)
- herbivory (1)
- heterostyly (1)
- hexoses (1)
- high density (1)
- high light (1)
- histidine-metal coordination (1)
- histone (1)
- histone modification (1)
- histone modifications, priming (1)
- holobiont (1)
- homoeotic (1)
- human growth (1)
- human-wildlife conflict (1)
- humic lake (1)
- hybridization capture (1)
- hydrochlorothiazide (1)
- hydrology (1)
- imaging (1)
- immunonutrition (1)
- individual niche specialization (1)
- induced pluripotent stem cells (1)
- inducible expression (1)
- insectivores (1)
- interaction (1)
- intestine (1)
- intra-specific trait variation (1)
- intraspecific trait variation (1)
- intraspezifische Merkmalsvariation (1)
- invertebrate macropores (1)
- ion mobility spectrometry (1)
- irbesartan (1)
- iron (1)
- iron-sulfur protein (1)
- jumping spider (1)
- kinematics (1)
- kinetic parameter (1)
- knemometry (1)
- l-cysteine desulfurase (1)
- laccase (1)
- lactic acid (1)
- lagoon (1)
- land-use change (1)
- latitudinal gradient (1)
- leading edge (1)
- learning networks plant (1)
- leucine amino peptidases (1)
- life-cycle (1)
- lifetime achievements (1)
- lignocellulose (1)
- linear growth (1)
- lipopolysaccharide (1)
- lipoproteins (1)
- livestock (1)
- lokale Anpassung (1)
- lower leg length (1)
- macropores (1)
- male body height (1)
- mammalian body (1)
- martini (1)
- maternal trauma (1)
- mechanosensation (1)
- membrane (1)
- membrane fractionation (1)
- meta-community (1)
- metabarcoding (1)
- metabolic engineering (1)
- metabolic network (1)
- metabolic networks (1)
- metabolites (1)
- metal-containing enzyme (1)
- metalloenzyme (1)
- methane (1)
- methanogens (1)
- methanol assimilation (1)
- methanotrophs (1)
- micorbicide (1)
- microRNA172 (1)
- microbial activity (1)
- microbial community (1)
- microgel (1)
- microparticles (1)
- microplastics (1)
- microplastics ingestion (1)
- microscopy (1)
- microtubule (1)
- microviscosity (1)
- mikrobielle Aktivität (1)
- mikrobielle Gemeinschaft (1)
- mini growth spurts (1)
- mitigation (1)
- mitigation measures (1)
- model (1)
- modifications (1)
- modified electrodes (1)
- modularity (1)
- molecular dynamics (1)
- molecular farming (1)
- molecularly imprinted polymer (1)
- molecularly imprinted polymers (1)
- molybdenum cofactor (Moco) (1)
- molybdo-flavoenzyme (1)
- mormyridae (1)
- morphometrics (1)
- mortality (1)
- motor neurons (1)
- mouse (1)
- mulifractional diffusion (1)
- multi-fraktionelle Diffusion (1)
- multi-omics (1)
- multipara (1)
- multiple (1)
- mutagenesis (1)
- mutants (1)
- nanoparticles (1)
- natural rotifer (1)
- neck evolution (1)
- neofunctionalization (1)
- neutral landscape models (1)
- next generation sequencing (1)
- nitrile (1)
- no threshold for stunting (1)
- nutrition (1)
- oil palm (1)
- optimal foraging (1)
- ordering of particles on the surface (1)
- organ (1)
- oxidase (1)
- oxidative Proteinmodifikationen (1)
- oxidative protein modifications (1)
- oxygen radicals (1)
- paired species (1)
- palaeovegetation (1)
- paleogenomics (1)
- papillae (1)
- parasites (1)
- parental education (1)
- parental educational status (1)
- parity (1)
- partial differential equations (1)
- particle-associated (1)
- pathogen host (1)
- pathogen host interaction (1)
- pavement cells image analysis (1)
- peptide (1)
- permafrost degradation (1)
- peroxidase mimics (1)
- peroxidases (1)
- personality (1)
- pharmaceutical dosage forms (1)
- phenols (1)
- phenomics (1)
- phenotyping (1)
- phylogenetic relationship (1)
- phylogeny (1)
- phylogeography (1)
- plan (1)
- plant (1)
- plant diversity (1)
- plant growth (1)
- plant-soil feedback (1)
- plants (1)
- plastid transformation (1)
- platinum nanoparticles (1)
- pollen (1)
- pollinator (1)
- polyelectrolyte multilayers (1)
- polypeptide (1)
- polystyrene (1)
- population (1)
- population density (1)
- population genetics (1)
- post-translational modifications (1)
- posttranslationale Modifikationen (1)
- potency (1)
- predation risk (1)
- predator (1)
- prey (1)
- primipara (1)
- process-based crop model (1)
- protein (1)
- protein fusion (1)
- protein phosphatase (1)
- psychological factors (1)
- psychosocial factors (1)
- rain-simulation (1)
- rank (1)
- rapid diagnosis (1)
- raptors (1)
- rate (RGR) (1)
- re-sequencing (1)
- recombinant production (1)
- redox (1)
- redox marker (1)
- reed (1)
- reference sequence (1)
- regression (1)
- regulation (1)
- relative expansion rate of growth (RER) (1)
- relative growth (1)
- reliability (1)
- renewable energy (1)
- resource (1)
- resource selection (1)
- resurrection plants (1)
- rheology (1)
- ribosome profiling (1)
- risk-reward tradeoff (1)
- rivers (1)
- rotifer (1)
- räumlich und zeitlich kontrollierte Wirkstoff-Freisetzung (1)
- salt diffusion (1)
- saprobic fungi (1)
- school children (1)
- secular trend (1)
- sediment core (1)
- sedimentary DNA (1)
- seismic activity (1)
- seismische Aktivität (1)
- selective autophagy (1)
- semiconductors (1)
- shape-memory polymers (1)
- short term growth (1)
- signalling (1)
- signals (1)
- single-molecule force spectroscopy (1)
- skeletal robustness (1)
- small mammals (1)
- social classification (1)
- social factors (1)
- social status (1)
- social systems (1)
- social-economic-political-emotional (1)
- sociality (1)
- socioeconomic status (1)
- soil aggregation (1)
- soil function (1)
- spatially and temporally controlled drug release (1)
- specific root surface (1)
- specificity (1)
- specifier proteins (1)
- sphingolipids (1)
- sphingosine-1-phosphate (1)
- statistical tools (1)
- step selection (1)
- stewartan (1)
- storm (1)
- strains (1)
- stress recovery (1)
- structure (1)
- subsea permafrost (1)
- sulfur (1)
- supergene (1)
- supervised learning (1)
- sustainable (1)
- synthase (1)
- talik (1)
- target enrichment (1)
- targeted assembly (1)
- taxis (1)
- termites (1)
- thermokarst (1)
- thermokarst lake (1)
- thiazolidine-4-carboxylic acid (1)
- thioproline (1)
- tiefe Biosphäre (1)
- tissue (1)
- toothed whales (1)
- trade-offs (1)
- training sample (1)
- trait variation (1)
- transfer (1)
- transgenic (1)
- translation (1)
- treeline (1)
- trophic cascades (1)
- tropical maize (1)
- turnover number (1)
- undernutrition (1)
- unterirdische Pflanzenfresser (1)
- urbanization (1)
- validity (1)
- video scan technology (1)
- water resources (1)
- water-in-water (1)
- weevil (1)
- western Eger Rift (1)
- westlichen Eger-Graben (1)
- wetlands (1)
- whole genome (1)
- wind energy (1)
- xenobiotic (1)
- zebrafish (1)
- zooplankton (1)
- ökologische Modellierung (1)
Institute
- Institut für Biochemie und Biologie (250) (remove)
Research on novel and advanced biomaterials is an indispensable step towards their applications in desirable fields such as tissue engineering, regenerative medicine, cell culture, or biotechnology. The work presented here focuses on such a promising material: polyelectrolyte multilayer (PEM) composed of hyaluronic acid (HA) and poly(L-lysine) (PLL). This gel-like polymer surface coating is able to accumulate (bio-)molecules such as proteins or drugs and release them in a controlled manner. It serves as a mimic of the extracellular matrix (ECM) in composition and intrinsic properties. These qualities make the HA/PLL multilayers a promising candidate for multiple bio-applications such as those mentioned above. The work presented aims at the development of a straightforward approach for assessment of multi-fractional diffusion in multilayers (first part) and at control of local molecular transport into or from the multilayers by laser light trigger (second part).
The mechanism of the loading and release is governed by the interaction of bioactives with the multilayer constituents and by the diffusion phenomenon overall. The diffusion of a molecule in HA/PLL multilayers shows multiple fractions of different diffusion rate. Approaches, that are able to assess the mobility of molecules in such a complex system, are limited. This shortcoming motivated the design of a novel evaluation tool presented here.
The tool employs a simulation-based approach for evaluation of the data acquired by fluorescence recovery after photobleaching (FRAP) method. In this approach, possible fluorescence recovery scenarios are primarily simulated and afterwards compared with the data acquired while optimizing parameters of a model until a sufficient match is achieved. Fluorescent latex particles of different sizes and fluorescein in an aqueous medium are utilized as test samples validating the analysis results. The diffusion of protein cytochrome c in HA/PLL multilayers is evaluated as well.
This tool significantly broadens the possibilities of analysis of spatiotemporal FRAP data, which originate from multi-fractional diffusion, while striving to be widely applicable. This tool has the potential to elucidate the mechanisms of molecular transport and empower rational engineering of the drug release systems.
The second part of the work focuses on the fabrication of such a spatiotemporarily-controlled drug release system employing the HA/PLL multilayer. This release system comprises different layers of various functionalities that together form a sandwich structure. The bottom layer, which serves as a reservoir, is formed by HA/PLL PEM deposited on a planar glass substrate. On top of the PEM, a layer of so-called hybrids is deposited. The hybrids consist of thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) -based hydrogel microparticles with surface-attached gold nanorods. The layer of hybrids is intended to serve as a gate that controls the local molecular transport through the PEM–solution-interface. The possibility of stimulating the molecular transport by near-infrared (NIR) laser irradiation is being explored.
From several tested approaches for the deposition of hybrids onto the PEM surface, the drying-based approach was identified as optimal. Experiments, that examine the functionality of the fabricated sandwich at elevated temperature, document the reversible volume phase transition of the PEM-attached hybrids while sustaining the sandwich stability. Further, the gold nanorods were shown to effectively absorb light radiation in the tissue- and cell-friendly NIR spectral region while transducing the energy of light into heat. The rapid and reversible shrinkage of the PEM-attached hybrids was thereby achieved. Finally, dextran was employed as a model transport molecule. It loads into the PEM reservoir in a few seconds with the partition constant of 2.4, while it spontaneously releases in a slower, sustained manner. The local laser irradiation of the sandwich, which contains the fluorescein isothiocyanate tagged dextran, leads to a gradual reduction of fluorescence intensity in the irradiated region.
The release system fabricated employs renowned photoresponsivity of the hybrids in an innovative setting. The results of the research are a step towards a spatially-controlled on-demand drug release system that paves the way to spatiotemporally controlled drug release.
The approaches developed in this work have the potential to elucidate the molecular dynamics in ECM and to foster engineering of multilayers with properties tuned to mimic the ECM. The work aims at spatiotemporal control over the diffusion of bioactives and their presentation to the cells.
We recently demonstrated that the sympathetic nervous system can be voluntarily activated following a training program consisting of cold exposure, breathing exercises, and meditation. This resulted in profound attenuation of the systemic inflammatory response elicited by lipopolysaccharide (LPS) administration. Herein, we assessed whether this training program affects the plasma metabolome and if these changes are linked to the immunomodulatory effects observed. A total of 224 metabolites were identified in plasma obtained from 24 healthy male volunteers at six timepoints, of which 98 were significantly altered following LPS administration. Effects of the training program were most prominent shortly after initiation of the acquired breathing exercises but prior to LPS administration, and point towards increased activation of the Cori cycle. Elevated concentrations of lactate and pyruvate in trained individuals correlated with enhanced levels of anti-inflammatory interleukin (IL)-10. In vitro validation experiments revealed that co-incubation with lactate and pyruvate enhances IL-10 production and attenuates the release of pro-inflammatory IL-1 beta and IL-6 by LPS-stimulated leukocytes. Our results demonstrate that practicing the breathing exercises acquired during the training program results in increased activity of the Cori cycle. Furthermore, this work uncovers an important role of lactate and pyruvate in the anti-inflammatory phenotype observed in trained subjects.
We recently demonstrated that the sympathetic nervous system can be voluntarily activated following a training program consisting of cold exposure, breathing exercises, and meditation. This resulted in profound attenuation of the systemic inflammatory response elicited by lipopolysaccharide (LPS) administration. Herein, we assessed whether this training program affects the plasma metabolome and if these changes are linked to the immunomodulatory effects observed. A total of 224 metabolites were identified in plasma obtained from 24 healthy male volunteers at six timepoints, of which 98 were significantly altered following LPS administration. Effects of the training program were most prominent shortly after initiation of the acquired breathing exercises but prior to LPS administration, and point towards increased activation of the Cori cycle. Elevated concentrations of lactate and pyruvate in trained individuals correlated with enhanced levels of anti-inflammatory interleukin (IL)-10. In vitro validation experiments revealed that co-incubation with lactate and pyruvate enhances IL-10 production and attenuates the release of pro-inflammatory IL-1 beta and IL-6 by LPS-stimulated leukocytes. Our results demonstrate that practicing the breathing exercises acquired during the training program results in increased activity of the Cori cycle. Furthermore, this work uncovers an important role of lactate and pyruvate in the anti-inflammatory phenotype observed in trained subjects.
The Fram Strait is an area with a relatively low and irregular distribution of diatom microfossils in surface sediments, and thus microfossil records are scarce, rarely exceed the Holocene, and contain sparse information about past richness and taxonomic composition. These attributes make the Fram Strait an ideal study site to test the utility of sedimentary ancient DNA (sedaDNA) metabarcoding. Amplifying a short, partial rbcL marker from samples of sediment core MSM05/5-712-2 resulted in 95.7% of our sequences being assigned to diatoms across 18 different families, with 38.6% of them being resolved to species and 25.8% to genus level. Independent replicates show a high similarity of PCR products, especially in the oldest samples. Diatom sedaDNA richness is highest in the Late Weichselian and lowest in Mid- and Late Holocene samples. Taxonomic composition is dominated by cold-water and sea-ice-associated diatoms and suggests several reorganisations - after the Last Glacial Maximum, after the Younger Dryas, and after the Early and after the Mid-Holocene. Different sequences assigned to, amongst others, Chaetoceros socialis indicate the detectability of intra-specific diversity using sedaDNA. We detect no clear pattern between our diatom sedaDNA record and the previously published IP25 record of this core, although proportions of pennate diatoms increase with higher IP25 concentrations and proportions of Nitzschia cf. frigida exceeding 2% of the assemblage point towards past sea-ice presence.
Ranging behavior of European rabbits (Oryctolagus cuniculus) in urban and suburban landscapes
(2020)
Various mammals, particularly carnivores, reportedly establish smaller home ranges in urban compared with rural areas. This may be because urban environments provide optimal resources within a small area, negating the requirement to range further, or because habitat fragmentation constrains ranging behavior. Comparable information on urban populations of herbivorous mammalian species (such as European rabbits) is scarce. To fill this knowledge gap, we radio-tracked 13 individuals (seven females and six males) equipped with radio collars in a suburban and an urban study site in the city of Frankfurt am Main in Germany during the reproductive season (March to September) of 2012. The study sites differed in levels of habitat fragmentation. We report the smallest home ranges ever described for this species, with mean 95% minimum convex polygons (MCPs) covering 0.50 ha, while no consistent differences between sites were uncovered. We occasionally tracked individuals crossing streets underground (in burrows), suggesting that streets may restrict the ranging behavior of rabbits-and possibly other burrowing species-to a much lesser extent than previously thought. We conclude that heterogeneous landscape structures, made up of a diverse mosaic of buildings, parks, and gardens, provide sufficient food and shelter in close proximity to burrows at both study sites. Therefore, our data support the hypothesis that optimal resources constrain ranges in this case rather than habitat fragmentation.
Although the use of stable transformation technology has led to great insight into gene function, its application in high-throughput studies remains arduous. Agro-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and protein interaction analysis, but this technique does not work efficiently in other plant species, including Arabidopsis thaliana. As an efficient high-throughput transient expression system is currently lacking in the model plant species A. thaliana, we developed a method that is characterized by high efficiency, reproducibility, and suitability for transient expression of a variety of functional proteins in A. thaliana and 7 other plant species, including Brassica oleracea, Capsella rubella, Thellungiella salsuginea, Thellungiella halophila, Solanum tuberosum, Capsicum annuum, and N. benthamiana. Efficiency of this method was independently verified in three independent research facilities, pointing to the robustness of this technique. Furthermore, in addition to demonstrating the utility of this technique in a range of species, we also present a case study employing this method to assess protein-protein interactions in the sucrose biosynthesis pathway in Arabidopsis.
Although the use of stable transformation technology has led to great insight into gene function, its application in high-throughput studies remains arduous. Agro-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and protein interaction analysis, but this technique does not work efficiently in other plant species, including Arabidopsis thaliana. As an efficient high-throughput transient expression system is currently lacking in the model plant species A. thaliana, we developed a method that is characterized by high efficiency, reproducibility, and suitability for transient expression of a variety of functional proteins in A. thaliana and 7 other plant species, including Brassica oleracea, Capsella rubella, Thellungiella salsuginea, Thellungiella halophila, Solanum tuberosum, Capsicum annuum, and N. benthamiana. Efficiency of this method was independently verified in three independent research facilities, pointing to the robustness of this technique. Furthermore, in addition to demonstrating the utility of this technique in a range of species, we also present a case study employing this method to assess protein-protein interactions in the sucrose biosynthesis pathway in Arabidopsis.
Completely water-based systems are of interest for the development of novel material for various reasons: On one hand, they provide benign environment for biological systems and on the other hand they facilitate effective molecular transport in a membrane-free environment. In order to investigate the general potential of aqueous two-phase systems (ATPSs) for biomaterials and compartmentalized systems, various solid particles were applied to stabilize all-aqueous emulsion droplets. The target ATPS to be investigated should be prepared via mixing of two aqueous solutions of water-soluble polymers, which turn biphasic when exceeding a critical polymer concentration. Hydrophilic polymers with a wide range of molar mass such as dextran/poly(ethylene glycol) (PEG) can therefore be applied. Solid particles adsorbed at the interfaces can be exceptionally efficient stabilizers forming so-called Pickering emulsions, and nanoparticles can bridge the correlation length of polymer solutions and are thereby the best option for water-in-water emulsions.
The first approach towards the investigation of ATPS was conducted with all aqueous dextran-PEG emulsions in the presence of poly(dopamine) particles (PDP) in Chapter 4. The water-in-water emulsions were formed with a PEG/dextran system via utilizing PDP as stabilizers. Studies of the formed emulsions were performed via laser scanning confocal microscope (CLSM), optical microscope (OM), cryo-scanning electron microscope (SEM) and tensiometry. The stable emulsions (at least 16 weeks) were demulsified easily via dilution or surfactant addition. Furthermore, the solid PDP at the water-water interface were crosslinked in order to inhibit demulsification of the Pickering emulsion. Transmission electron microscope (TEM) and scanning electron microscope (SEM) were used to visualize the morphology of PDP before and after crosslinking. PDP stabilized water-in-water emulsions were utilized in the following Chapter 5 to form supramolecular compartmentalized hydrogels. Here, hydrogels were prepared in pre-formed water-in-water emulsions and gelled via α-cyclodextrin-PEG (α-CD-PEG) inclusion complex formation. Studies of the formed complexes were performed via X-ray powder diffraction (XRD) and the mechanical properties of the hydrogels were measured with oscillatory shear rheology. In order to verify the compartmentalized state and its triggered decomposition, hydrogels and emulsions were assessed via OM, SEM and CLSM. The last chapter broadens the investigations from the previous two systems by utilizing various carbon nitrides (CN) as different stabilizers in ATPS. CN introduces another way to trigger demulsification, namely irradiation with visible light. Therefore, emulsification and demulsification with various triggers were probed. The investigated all aqueous multi-phase systems will act as model for future fabrication of biocompatible materials, cell micropatterning as well as separation of compartmentalized systems.
Ecosystems with highly pulsed water supply must be better understood as climate change may increase frequency and severity of intense storms, droughts and floods. Here we collected data over 3 years (2016-2018) in the episodic wetland outflow channel (Aluize), Banhine National Park, in which the system state changed from dry to wet to dry. Field sampling included vegetation records, small-scale vegetation zoning, the seed bank and water and soil quality. The same main plant species were found in both dry and wet conditions across the riverbed of the outflow channel. We found only very few diaspores of plants in the soil after prolonged drought. In the subsequent flooded state, we examined very dense vegetation on the water surface, which was dominated by the gramineous species Paspalidium obtusifolium. This species formed a compact floating mat that was rooted to the riverbed. The Cyperaceae Bolboschoenus glaucus showed high clonal growth in the form of root tubers, which likely serve as important food reservoir during drought. Soil and water analyses do not indicate a limitation by nutrients. We outline how resident people may change the plant community structure with an increasing practice of setting fire to the meadows in the dried-up riverbed to facilitate plant regrowth as food for their livestock.
Ecosystems with highly pulsed water supply must be better understood as climate change may increase frequency and severity of intense storms, droughts and floods. Here we collected data over 3 years (2016-2018) in the episodic wetland outflow channel (Aluize), Banhine National Park, in which the system state changed from dry to wet to dry. Field sampling included vegetation records, small-scale vegetation zoning, the seed bank and water and soil quality. The same main plant species were found in both dry and wet conditions across the riverbed of the outflow channel. We found only very few diaspores of plants in the soil after prolonged drought. In the subsequent flooded state, we examined very dense vegetation on the water surface, which was dominated by the gramineous species Paspalidium obtusifolium. This species formed a compact floating mat that was rooted to the riverbed. The Cyperaceae Bolboschoenus glaucus showed high clonal growth in the form of root tubers, which likely serve as important food reservoir during drought. Soil and water analyses do not indicate a limitation by nutrients. We outline how resident people may change the plant community structure with an increasing practice of setting fire to the meadows in the dried-up riverbed to facilitate plant regrowth as food for their livestock.