Refine
Year of publication
- 2008 (459) (remove)
Document Type
- Article (206)
- Doctoral Thesis (116)
- Conference Proceeding (39)
- Monograph/Edited Volume (36)
- Postprint (36)
- Preprint (11)
- Other (6)
- Master's Thesis (3)
- Review (3)
- Habilitation Thesis (2)
Language
- English (459) (remove)
Keywords
- Chitooligosaccharide (3)
- Chitooligosaccharides (3)
- Erdbeben (3)
- Seismology (3)
- magnetic fields (3)
- Array Seismology (2)
- Arrayseismologie (2)
- Chile (2)
- Chitinase (2)
- Earthquake (2)
- Glycosylation (2)
- Magnetfelder (2)
- Mass Spectrometry (2)
- Massenspektrometrie (2)
- Metabolomics (2)
- Metallnitride (2)
- Seismologie (2)
- Subduction (2)
- Subduktion (2)
- array seismology (2)
- climate change (2)
- earthquake (2)
- experimental linguistics (2)
- focus asymmetries (2)
- metal nitrides (2)
- nucleation (2)
- potato (2)
- 3D Finite Element (1)
- 53C12 (1)
- 53C27 (1)
- ABC triblock copolymer (1)
- ATP (1)
- Abflussbildungsprozesse (1)
- Additive (1)
- Adsorbatschwingungen (1)
- Afro-Asiatic (1)
- Amazonas (1)
- Amazonia (1)
- Amin (1)
- Amygdala (1)
- Angsterkrankung (1)
- Antibiotic alternatives (1)
- Antibiotic resistance (1)
- Antibiotikaersatz (1)
- Antibiotikaresistenz (1)
- Antipredator Behaviour (1)
- Apyrase (1)
- Arabidopsis thaliana (1)
- Arbeitslosigkeit (1)
- Arbeitsplatz (1)
- Arbeitsplatzphobie (1)
- Arbeitsunfähigkeit (1)
- Arktis (1)
- Array Seismologie (1)
- AtIpk2 beta (1)
- Atom chip (1)
- Attraktorrekonstruktion (1)
- Automatentheorie (1)
- Avian Predation (1)
- Basic Service (1)
- Basisemotion (1)
- Benetzungsübergang (1)
- Black Sea (1)
- Bose-Einstein condensation (1)
- Brownian motion (1)
- Bruchverfolgung (1)
- Bryophytes (1)
- CAP (1)
- Calciumcarbonat (1)
- Carbohydrates (1)
- Carbonate (1)
- Carbonates (1)
- Carbonitrides (1)
- Categories of stratified spaces (1)
- Chemical Synthesis (1)
- Chemische Synthese (1)
- Chitolectins (1)
- Chitolektine (1)
- Chitosan (1)
- Chloroplast transformation (1)
- Chloroplastentransformation (1)
- Clauses (1)
- Clonal growth (1)
- Cloze predictability (1)
- Cluster (1)
- Co-occurrence probability (1)
- Coherence (1)
- Colombia (1)
- Computation Tree Logic (1)
- Computerlinguistik (1)
- Conjunctive Normal Form (1)
- Contrastive stress (1)
- Cosmogenic Nuclides (1)
- Covariate Shift (1)
- Datenanalyse (1)
- Datenbank (1)
- Deichbruch (1)
- Description Logics (1)
- Deskriptive Logik (1)
- Deutsch (1)
- Deutschland (1)
- Dezentralisierung (1)
- Diagenese (1)
- Diagenesis (1)
- Diaspora (1)
- Dichtematrixtheorie offener Systeme (1)
- Disjunctive Normal Form (1)
- Diskursverstehen (1)
- Dissonanz (1)
- EKP (1)
- ERP (1)
- Eastern Europe (1)
- Echtzeitanwendung (1)
- Elbe (1)
- Elbe River (1)
- Elektronenbeschleunigung (1)
- Emotion (1)
- Emulsion Polymerization (1)
- Emulsionspolymerisation (1)
- Endliche Automaten (1)
- European Bioinformatics Institute (1)
- Evolution (1)
- Evolutionsgleichung (1)
- Evolutionspsychologie (1)
- Experiment (1)
- Feedback control (1)
- Festphasensynthese (1)
- Figurative Sprachverarbeitung (1)
- Figurative language processing (1)
- Fiskalausgleich (1)
- Fiskaltransfers (1)
- Flarephysik (1)
- Fließwege (1)
- Flood (1)
- Flood polders (1)
- Fluorpolymere (1)
- Flutpolder (1)
- Food Patch (1)
- Fouriertransformation (1)
- Full Adder (1)
- Fungi (1)
- Galaxien (1)
- Gas Chromatography (1)
- Gaschromatographie (1)
- Gefährdungskarten (1)
- Gender (1)
- Generation (1)
- Genotype Inference (1)
- Geodynamic Modeling (1)
- Geodynamiche Modellierung (1)
- Geodäten (1)
- German (1)
- Germany (1)
- Gibbs state (1)
- Gleichstellung (1)
- Global coupling (1)
- Globaler Wandel (1)
- Glycosylierung (1)
- Glykosylierung (1)
- Governance (1)
- Great Barrier Reef (1)
- Grundsteuer (1)
- Grundwasser (1)
- HPLC (1)
- Haplotype Inference (1)
- Harvest Rate (1)
- Hasso-Plattner-Institut (1)
- Hasso-Plattner-Institute (1)
- Heterophase Polymerization (1)
- Hochwasser (1)
- Holzprodukte (1)
- IP3 (1)
- IR excitation (1)
- IR-Anregung (1)
- Input Validation (1)
- Integration (1)
- Kartoffel (1)
- Kinetics (1)
- Klassiche Simulationen (1)
- Klimawandel (1)
- Knospung (1)
- Kohlenhydrate (1)
- Kohlenstoffnitride (1)
- Kohlenstoffspeicherung (1)
- Kohärenz (1)
- Kolumbien (1)
- Komplexe Systeme (1)
- Konsonanz (1)
- Kristallisation (1)
- Kulturelle Identität (1)
- Kultureller Wandel (1)
- Küstenerosion (1)
- Language Acquisition (1)
- Last Glacial (1)
- Lebenszyklusmodell (1)
- Letztes Glazial (1)
- Liquiñe-Ofqui Störungszone (1)
- Liquiñe-Ofqui fault zone (1)
- Lithospheric Deformation (1)
- Lithosphärische Deformation (1)
- Lokalsteuern (1)
- Loop space (1)
- MALDI-TOF mass spectrometry (1)
- Machine Learning (1)
- Magnetische Felder (1)
- Magnetohydrodynamik (1)
- Markov Wahrscheinlichkeiten (1)
- Martin-Dynkin boundary (1)
- Maschinelles Lernen (1)
- Maximum Parsimony (1)
- Maximum Parsimony tree (1)
- Mehrebenenanalyse (1)
- Membranröhrchen (1)
- Metaboliten (1)
- Metabolites (1)
- Metalloxide (1)
- Middle East (1)
- Migration (1)
- Miocene (1)
- Miozän (1)
- Mizellen (1)
- Modellkomplexität (1)
- Momententensoren (1)
- Morphometrie (1)
- Morphometry (1)
- Multi Task Learning (1)
- Multi-Task-Lernen (1)
- Multiproxy-Untersuchung (1)
- Nanopartikel (1)
- Natural Pause Hierarchy (1)
- Natürliche Pausenhierarchie (1)
- Netzwerke (1)
- Neuronal synchrony (1)
- Neuronale Synchronisation (1)
- Nichtwässrige Synthese (1)
- Nitrogen deposition (1)
- Noisy oscillators (1)
- Nukleation (1)
- Nukleierung (1)
- Nukleosidase (1)
- Nährstoffbilanz (1)
- Nährstoffkeislauf (1)
- Oberflächenstrukturen (1)
- Oligosaccharides (1)
- Ontologie (1)
- Ontologies (1)
- Optimale Kontrolltheorie (1)
- Organosilica (1)
- Osteuropa (1)
- Paläoklima (1)
- Partizipationsstörung (1)
- Pausen (1)
- Pauses (1)
- Permafrost (1)
- Pflanzen (1)
- Phage lysins (1)
- Phagenlysine (1)
- Phasentrennung (1)
- Photodesorption (1)
- Phytopathogens (1)
- Pinus sylvestris (1)
- Plankton (1)
- Point process (1)
- Polarisation (1)
- Polder (1)
- Polymerase Chain Reaction Experiment (1)
- Pragmatik (1)
- Predation Risk (1)
- Profilanalysen (1)
- Profiling (1)
- Prosody (1)
- Proteinphosphorylation (1)
- QTL Analyse (1)
- QTL mapping (1)
- Quantendynamische Simulationen (1)
- Quercus (1)
- Receiver Functions (1)
- Receiver Funktionen (1)
- Regenerierung (1)
- Regenwald (1)
- Regulation (1)
- Räuber-Beute Beziehungen (1)
- Rückkopplungskontrolle (1)
- SWIM (1)
- Sample Selection Bias (1)
- San Andreas Fault (1)
- San Andreas Verwerfung (1)
- Sardinia (1)
- Sardinien (1)
- Schadenabschätzung (1)
- Schwarzes Meer (1)
- Schwingungsanregung (1)
- Sedimentologie (1)
- Sedimentology (1)
- Selektionsbias (1)
- Selektivität (1)
- Semantic Search (1)
- Semantic Web (1)
- Semantik Web (1)
- Semantische Suche (1)
- Sichuan (1)
- Simulation (1)
- Solid phase synthesis (1)
- Solution phase synthesis (1)
- South Chile (1)
- Sparraten (1)
- Species richness (1)
- Spracherwerb (1)
- Sprachverarbeitung (1)
- Späte Sterne (1)
- Stellare Aktivität (1)
- Stoffwechsel (1)
- Strain Localization (1)
- Strings (1)
- Subduktionszone (1)
- Succession (1)
- Sumatra (1)
- Supernovaüberreste (1)
- Supraleiter (1)
- Synthese (1)
- Synthesemethoden (1)
- Synthesis (1)
- Synthetic methods (1)
- Systembiologie (1)
- Süd Chile (1)
- Tauziehen (1)
- Tectonics (1)
- Teilchenbildung (1)
- Tektonik (1)
- Templat (1)
- Templatierung (1)
- Terminologische Logik (1)
- Thermochronologie (1)
- Thermokarst (1)
- Tiefer See (1)
- Transkriptionfaktorgenen (1)
- Trichloracetimidate (1)
- Trichloroacetimidates (1)
- Tsunami (1)
- Turbulenz (1)
- Understorey (1)
- Unsicherheitsanalyse (1)
- Vacuolar h+-atpase (1)
- Vascular plants (1)
- Verformungslokalisierung (1)
- Verteilungsunterschied (1)
- Vesikel (1)
- Vorelandbecken (1)
- Waldwachstumsmodell 4C (1)
- Wassergütesimulation (1)
- Wenchuan (1)
- X-rays: general (1)
- acrylic acid esters (1)
- action language (1)
- additives (1)
- adsorbate vibrations (1)
- afro-asiatic (1)
- alignment error (1)
- amine (1)
- amygdala (1)
- answer set programming (1)
- antimalarial activity (1)
- anxiety disorder (1)
- apyrase (1)
- arabidopsis (1)
- arabidopsis thaliana (1)
- arctic (1)
- argument / adjunct focus (1)
- argument/adjunct focus (1)
- assoziative Photodesorption (1)
- atom chip (1)
- atomic processes (1)
- automata theory (1)
- basic emotion (1)
- bidirectional intracellular transport (1)
- bidirektionaler intrazellulärer Transport (1)
- biological network (1)
- biological network model (1)
- biomechanical effect (1)
- boundary value problems (1)
- budding (1)
- burnout syndrome (1)
- cAMP analog (1)
- calcium carbonate (1)
- camp binding-sites (1)
- carbohydrate esters (1)
- carbon sequestration (1)
- chirality (1)
- classical dynamics (1)
- cleft (1)
- clusters (1)
- coastal erosion (1)
- comparative urban studies (1)
- complex systems (1)
- composite service (1)
- computational linguistics (1)
- conditioned (1)
- conditioned Feller diffusion (1)
- confinement (1)
- consonance (1)
- cooperative phenomena (1)
- coronal currents (1)
- cosmology : large-scale structure of the Universe (1)
- critical and subcritical Dawson-Watanabe process (1)
- crystallization (1)
- cultural change (1)
- cultural identity (1)
- cyclic-amp (1)
- damage estimation (1)
- data analysis (1)
- data scarcity (1)
- database (1)
- decentralization (1)
- decision making (1)
- deep lake (1)
- diaspora (1)
- diaspore weight (1)
- dike breach (1)
- discourse comprehension (1)
- dispersal potential (1)
- dissonance (1)
- drosophila-melanogaster (1)
- dustdynamic (1)
- dynamik (1)
- e-Learning (1)
- ecology (1)
- economic disparities (1)
- education return (1)
- efficient (1)
- electron acceleration (1)
- electronic tool integration (1)
- ellipticity of corners operators (1)
- emotional distance (1)
- emotional expression (1)
- emulsion polymerization (1)
- encke (1)
- endoplasmic reticulum (1)
- enzymatic esterification (1)
- equal opportunities (1)
- evolution (1)
- evolution equation (1)
- evolutionary psychology (1)
- experiment (1)
- eye movement (1)
- fatty acid amide hydrolase (1)
- female runner (1)
- finite-state automata (1)
- fiscal transfers (1)
- flowpath (1)
- fluid secretion (1)
- fluorinated polymers (1)
- focus copula (1)
- foliations (1)
- foreland basin (1)
- forest growth model 4C (1)
- forest specialist (1)
- forestry (1)
- fourier transformation (1)
- frequency (1)
- functionalized (1)
- funktionalisiert (1)
- galaxies (1)
- galaxies : intergalactic medium (1)
- gender (1)
- generalist emergent group (1)
- generation (1)
- genome annotation (1)
- geodesics (1)
- global change (1)
- globale Kupplung (1)
- governance (1)
- ground water (1)
- hazard maps (1)
- heliotropic (1)
- heliotropisch (1)
- human capital (1)
- hydrodynamic modelling (1)
- hydrodynamische Simulation (1)
- hydrological processes (1)
- income contingent loans (1)
- incomplete lineage sorting (1)
- information (1)
- injury symptom (1)
- inositol phosphate (1)
- instrumentation: photometers (1)
- instrumentation: spectrographs (1)
- integration (1)
- intergalactic medium (1)
- internal transcribe spacer (1)
- intonation (1)
- joziknipholones (1)
- kinetik (1)
- kooperative Phänomene (1)
- koronale Stromsysteme (1)
- kosmogene Nuklide (1)
- late-type stars (1)
- life cycle model (1)
- lifetime income (1)
- linkage disequilibrium (1)
- local Seismicity (1)
- lokale Seismitität (1)
- magnetic (1)
- magnetic noise (1)
- magnetisch (1)
- magnetisches Rauschen (1)
- magnetohydrodynamics (1)
- markov chains (1)
- mathematical simulation models (1)
- mathematische Simulationsmodelle (1)
- medical student (1)
- membrane tube (1)
- metabolic (1)
- metabolisch (1)
- metabolism (1)
- metabolite profile (1)
- metal oxides (1)
- micelles (1)
- microarray data (1)
- microstructured surface (1)
- microstrukturierte Oberfläche (1)
- model complexity (1)
- molecular motors (1)
- molecular network (1)
- molekulare Motoren (1)
- multi-proxy approach (1)
- multicompartment micelle (1)
- multilevel analysis (1)
- multitype measure-valued branching processes (1)
- municipal government (1)
- nano (1)
- nanoparticles (1)
- natural language processing (1)
- natural products (1)
- networks (1)
- neue Institutionentheorie (1)
- new institutional theory (1)
- nonaqueous synthesis (1)
- northern Andes (1)
- nucleosidase (1)
- nutrient budget (1)
- nutrient cycle (1)
- nördliche Anden (1)
- open system density matrix theory (1)
- optimal control theory (1)
- organosilica (1)
- overuse injury (1)
- p-Branen (1)
- p-branes (1)
- pairwise distance (1)
- paleoclimate (1)
- partial correlation (1)
- participation disorder (1)
- particle formation (1)
- permafrost (1)
- personal relationships (1)
- phase separation (1)
- phase space reconstruction (1)
- phosphorylation (1)
- phylogenetic inference (1)
- phylogeographic structure (1)
- physics of flares (1)
- physiological solution (1)
- plankton (1)
- plants (1)
- plasma-membrane (1)
- polarity focus (1)
- polarization (1)
- polder (1)
- polyurethane foam (1)
- porous (1)
- porös (1)
- pragmatics (1)
- predator-prey relationships (1)
- predicate focus (1)
- prime pair (1)
- primer pair design (1)
- principal symbolic hierarchies (1)
- professional commitment (1)
- propagation (1)
- property tax (1)
- prosody (1)
- public management (1)
- pure parsimony (1)
- quantification protocol (1)
- quantum dynamics (1)
- quasars : absorption lines (1)
- radiation mechanisms: thermal (1)
- rainforest (1)
- reactive templating (1)
- real-time application (1)
- reduced major axis (1)
- regional integration (1)
- regions (1)
- regulation (1)
- resistance (1)
- rest service (1)
- reversible addition fragmentation chain transfer (RAFT) (1)
- ringe (1)
- rings (1)
- risk pattern (1)
- rupture (1)
- salivary gland (1)
- saturn (1)
- saving rates (1)
- schrumpfende Städte (1)
- selectivity (1)
- service orchestration (1)
- sick leave (1)
- significant edge (1)
- similarity (1)
- solanum (1)
- soziale Beziehungen (1)
- spatially explicit model (1)
- specific prime pair (1)
- spin geometry (1)
- staub (1)
- stellar activity (1)
- stochastic processes (1)
- stochastische Prozesse (1)
- stocking capacity (1)
- stress partitioning (1)
- stress tolerance (1)
- structure elucidation (1)
- subduction zone (1)
- subsurface flow (1)
- superconductors (1)
- supercritical carbon dioxide (scCO₂) (1)
- supernova remnants (1)
- surface features (1)
- surfactant (1)
- systemsbiology (1)
- teleseismic rupture tracking (1)
- teleseismische Bruchverfolgung (1)
- template (1)
- tertiary education (1)
- thermochronology (1)
- thermokarst (1)
- thermoresponsive polymer (1)
- topography (1)
- transcription factor genes (1)
- transgenic tobacco (1)
- tug-of-war (1)
- tuition fees (1)
- turbulence (1)
- ultraviolet: general (1)
- uncertainty analysis (1)
- unemployment (1)
- ungesättigte Zone (1)
- urban decline (1)
- urban regeneration (1)
- verbal irony (1)
- verbale Ironie (1)
- vergleichende Stadtforschung (1)
- verrauschte Oszillatoren (1)
- vesicle (1)
- vibrational control (1)
- vibrational excitation (1)
- vocational education (1)
- volcanic ash soils (1)
- vulkanische Ascheböden (1)
- water balance (1)
- water quality modelling (1)
- wetting transition (1)
- wood products (1)
- workplace (1)
- workplace phobia (1)
- x-rays: general (1)
- Ähnlichkeit (1)
- Öffentliche Verwaltung (1)
- Ökologie (1)
- überkritisches Kohlendioxid (scCO₂) (1)
- γ -rays: general (1)
- fixation duration (1)
Institute
- Institut für Chemie (82)
- Institut für Biochemie und Biologie (73)
- Department Psychologie (33)
- Extern (28)
- Institut für Anglistik und Amerikanistik (27)
- Institut für Künste und Medien (21)
- Institut für Mathematik (20)
- Wirtschaftswissenschaften (20)
- Department Linguistik (19)
- Institut für Physik und Astronomie (19)
We establish elements of a new approch to ellipticity and parametrices within operator algebras on a manifold with higher singularities, only based on some general axiomatic requirements on parameter-dependent operators in suitable scales of spaces. The idea is to model an iterative process with new generations of parameter-dependent operator theories, together with new scales of spaces that satisfy analogous requirements as the original ones, now on a corresponding higher level. The “full” calculus is voluminous; so we content ourselves here with some typical aspects such as symbols in terms of order reducing families, classes of relevant examples, and operators near the conical exit to infinity.
The aim of the present study was to investigate the test-retest reliability of the olfactory detection threshold subtest of the Sniffin" Sticks test battery, if administered repeatedly on 4 time points. The detection threshold test was repeatedly conducted in 64 healthy subjects. On the first testing session, the threshold test was accomplished 3 times (T(1) = 0 min, T(2) = 35 min, and T(3) = 105 min), representing a short-term testing. A fourth threshold test was conducted on a second testing session (T(4) = 35.1 days after the first testing session), representing a long-term testing. The average scores for olfactory detection threshold for n-butanol did not differ significantly across the 4 points of time. The test-retest reliability (Pearson"s r) between the 4 time points of threshold testing were in a range of 0.43-0.85 (P < 0.01). These results support the notion that the olfactory detection threshold test is a highly reliable method for repeated olfactory testing, even if the test is repeated more than once per day and over a long-term period. It is concluded that the olfactory detection threshold test of the Sniffin" Sticks is suitable for repeated testing during experimental or clinical studies.
Oidium pedaliacearum sp. nov. (; O. sesami, nom. inval.) and Podosphaera macrospora comb. et stat. nov. (; Sphaerotheca alpina f. macrospora) are introduced, and the taxonomy and distribution of Erysiphe celosiae is discussed. New host species and new collections of Erysiphe cruciferarum (on Cleome hassleriana), E. flexuosa (on Aesculus hippocastanum), E. hedwigii (on Viburnum carlesii), E. heraclei (on Tinguarra montana), E. cf. macleayae (on Macleaya cordata), E. prunastri (on Prunus cerasifera), E. sedi (on Sedum aff. spectabilis), E. trifolii (on Trigonella caerulea), Golovinomyces cichoracearum (on Argyranthemum pinnatifidum subsp. succulentum), G. cf. hydrophyllacearum (on Nemophila menziesii), G. orontii (on Nolana spp.), G. cf. orontii (on Tiarella cordifolia), Neoerysiphe cumminsiana (on Bidens cf. ferulifolia), Oidium clitoriae (on Clitoria ternatea), O. cf. hortensiae (on Philadelphus coronarius), O. pedilanthi (on Pedilanthus tithymaloides), Oidium (Pseudoidium) sp. (on Utricularia alpina), Podosphaera sp. (on Bergia capensis), Sawadaea bicornis (on Acer platanoides) and S. tulasnei (on Acer ginnala and A. tatarica) are recorded from France, Germany, Greece and Mexico.
We propose a network structure-based model for heterosis, and investigate it relying on metabolite profiles from Arabidopsis. A simple feed-forward two-layer network model (the Steinbuch matrix) is used in our conceptual approach. It allows for directly relating structural network properties with biological function. Interpreting heterosis as increased adaptability, our model predicts that the biological networks involved show increasing connectivity of regulatory interactions. A detailed analysis of metabolite profile data reveals that the increasing-connectivity prediction is true for graphical Gaussian models in our data from early development. This mirrors properties of observed heterotic Arabidopsis phenotypes. Furthermore, the model predicts a limit for increasing hybrid vigor with increasing heterozygosity—a known phenomenon in the literature.
The boundary paradigm (Rayner, 1975) with a novel preview manipulation was used to examine the extent of parafoveal processing of words to the right of fixation. Words n + 1 and n + 2 had either correct or incorrect previews prior to fixation (prior to crossing the boundary location). In addition, the manipulation utilized either a high or low frequency word in word n + 1 location on the assumption that it would be more likely that n + 2 preview effects could be obtained when word n + 1 was high frequency. The primary findings were that there was no evidence for a preview benefit for word n + 2 and no evidence for parafoveal-on-foveal effects when word n + 1 is at least four letters long. We discuss implications for models of eye-movement control in reading.
The boundary paradigm (Rayner, 1975) with a novel preview manipulation was used to examine the extent of parafoveal processing of words to the right of fixation. Words n+1 and n+2 had either correct or incorrect previews prior to fixation (prior to crossing the boundary location). In addition, the manipulation utilized either a high or low frequency word in word n+1 location on the assumption that it would be more likely that n+2 preview effects could be obtained when word n+1 was high frequency. The primary findings were that there was no evidence for a preview benefit for word n+2 and no evidence for parafoveal-on-foveal effects when word n+1 is at least four letters long. We discuss implications for models of eye-movement control in reading.
QuantPrime
(2008)
Background
Medium- to large-scale expression profiling using quantitative polymerase chain reaction (qPCR) assays are becoming increasingly important in genomics research. A major bottleneck in experiment preparation is the design of specific primer pairs, where researchers have to make several informed choices, often outside their area of expertise. Using currently available primer design tools, several interactive decisions have to be made, resulting in lengthy design processes with varying qualities of the assays.
Results
Here we present QuantPrime, an intuitive and user-friendly, fully automated tool for primer pair design in small- to large-scale qPCR analyses. QuantPrime can be used online through the internet http://www.quantprime.de/ or on a local computer after download; it offers design and specificity checking with highly customizable parameters and is ready to use with many publicly available transcriptomes of important higher eukaryotic model organisms and plant crops (currently 295 species in total), while benefiting from exon-intron border and alternative splice variant information in available genome annotations. Experimental results with the model plant Arabidopsis thaliana, the crop Hordeum vulgare and the model green alga Chlamydomonas reinhardtii show success rates of designed primer pairs exceeding 96%.
Conclusion
QuantPrime constitutes a flexible, fully automated web application for reliable primer design for use in larger qPCR experiments, as proven by experimental data. The flexible framework is also open for simple use in other quantification applications, such as hydrolyzation probe design for qPCR and oligonucleotide probe design for quantitative in situ hybridization. Future suggestions made by users can be easily implemented, thus allowing QuantPrime to be developed into a broad-range platform for the design of RNA expression assays.