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Electrochemical methods offer great promise in meeting the demand for user-friendly on-site devices for monitoring important parameters. The food industry often runs own lab procedures, for example, for mycotoxin analysis, but it is a major goal to simplify analysis, linking analytical methods with smart technologies. Enzyme-linked immunosorbent assays, with photometric detection of 3,3',5,5'-tetramethylbenzidine (TMB), form a good basis for sensitive detection. To provide a straightforward approach for the miniaturization of the detection step, we have studied the pitfalls of the electrochemical TMB detection. By cyclic voltammetry it was found that the TMB electrochemistry is strongly dependent on the pH and the electrode material. A stable electrode response to TMB could be achieved at pH 1 on gold electrodes. We created a smartphone-based, electrochemical, immunomagnetic assay for the detection of ochratoxin A in real samples, providing a solid basis for sensing of further analytes.
Solar filaments often erupt partially. Although how they split remains elusive, the splitting process has the potential of revealing the filament structure and eruption mechanism. Here we investigate the pre-eruption splitting of an apparently single filament and its subsequent partial eruption on 2012 September 27. The evolution is characterized by three stages with distinct dynamics. During the quasi-static stage, the splitting proceeds gradually for about 1.5 hr, with the upper branch rising at a few kilometers per second and displaying swirling motions about its axis. During the precursor stage that lasts for about 10 minutes, the upper branch rises at tens of kilometers per second, with a pair of conjugated dimming regions starting to develop at its footpoints; with the swirling motions turning chaotic, the axis of the upper branch whips southward, which drives an arc-shaped extreme-ultraviolet front propagating in a similar direction. During the eruption stage, the upper branch erupts with the onset of a C3.7-class two-ribbon flare, while the lower branch remains stable. Judging from the well-separated footpoints of the upper branch from those of the lower one, we suggest that the pre-eruption filament processes a double-decker structure composed of two distinct flux bundles, whose formation is associated with gradual magnetic flux cancellations and converging photospheric flows around the polarity inversion line.
Glaubensfragen
(2021)
Der Artikel gibt einen Überblick über die verschiedenen Phasen von siebzig Jahren christlich-jüdischer Dialog und blickt in die Zukunft. Akzeptieren Christen die Konsequenzen, die aus der Immanenz des Judentums in ihrer Religion folgen? Vor allem in zentralen Handlungsfeldern der systematischen Theologie bleibt viel Raum für Entwicklung, allen voran der Christologie.
Although distally steepened carbonate ramps have been studied by numerous researchers, the processes that control the development of these carbonate systems, including tectonics, differential carbonate production along the ramp profile, or antecedent physiography of the slopes, are an ongoing discussion. We use a stratigraphic forward model to test different hypotheses to unravel controls over distally steepened ramp development, referenced to the well-known Upper Miocene Menorca carbonate ramp (Spain). Sensitivity tests show that distally steepened ramps develop under complex interaction among accommodation, carbonate production and sediment transport parameters. Ramp slope initiation is favoured by still stands and falls of sea-level, in a setting with high-frequency sea-level fluctuations with amplitude between 20 m and 40 m. Low-frequency and higher amplitude sea-level fluctuations of about 115 m tend to form models with no significant slope development. The impact of antecedent slope on the geometry of ramps is determined by the paleoslope inclination, with flat to subhorizontal paleosurfaces resulting in ramps that mirror the antecedent slope. In contrast, steeper paleosurfaces tend to result in ramps with well-defined slopes. Our models, therefore, show that the ramp profile becomes more influenced by the depth constraints on the carbonate sediment producers than by the geometry of the underlying topography as the inclination of the paleosurface increases. The presented models also show that seagrass-dominated shallow carbonate production tends to result in steep slopes due to the low-transport characteristic imposed by seagrass trapping. This steepness can, however, be altered by the introduction of high transport sediment grains from deeper carbonate producers, which fill the slopes and more distal sections of the ramp profile.
Non-local muscle fatigue effects on muscle strength, power, and endurance in healthy individuals
(2021)
Background
The fatigue of a muscle or muscle group can produce global responses to a variety of systems (i.e., cardiovascular, endocrine, and others). There are also reported strength and endurance impairments of non-exercised muscles following the fatigue of another muscle; however, the literature is inconsistent.
Objective
To examine whether non-local muscle fatigue (NLMF) occurs following the performance of a fatiguing bout of exercise of a different muscle(s).
Design
Systematic review and meta-analysis.
Search and Inclusion
A systematic literature search using a Boolean search strategy was conducted with PubMed, SPORTDiscus, Web of Science, and Google Scholar in April 2020, and was supplemented with additional 'snowballing' searches up to September 2020. To be included in our analysis, studies had to include at least one intentional performance measure (i.e., strength, endurance, or power), which if reduced could be considered evidence of muscle fatigue, and also had to include the implementation of a fatiguing protocol to a location (i.e., limb or limbs) that differed to those for which performance was measured. We excluded studies that measured only mechanistic variables such as electromyographic activity, or spinal/supraspinal excitability. After search and screening, 52 studies were eligible for inclusion including 57 groups of participants (median sample = 11) and a total of 303 participants.
Results
The main multilevel meta-analysis model including all effects sizes (278 across 50 clusters [median = 4, range = 1 to 18 effects per cluster) revealed a trivial point estimate with high precision for the interval estimate [- 0.02 (95% CIs = - 0.14 to 0.09)], yet with substantial heterogeneity (Q((277)) = 642.3, p < 0.01), I-2 = 67.4%). Subgroup and meta-regression analyses showed that NLMF effects were not moderated by study design (between vs. within-participant), homologous vs. heterologous effects, upper or lower body effects, participant training status, sex, age, the time of post-fatigue protocol measurement, or the severity of the fatigue protocol. However, there did appear to be an effect of type of outcome measure where both strength [0.11 (95% CIs = 0.01-0.21)] and power outcomes had trivial effects [- 0.01 (95% CIs = - 0.24 to 0.22)], whereas endurance outcomes showed moderate albeit imprecise effects [- 0.54 (95% CIs = - 0.95 to - 0.14)].
Conclusions
Overall, the findings do not support the existence of a general NLMF effect; however, when examining specific types of performance outcomes, there may be an effect specifically upon endurance-based outcomes (i.e., time to task failure). However, there are relatively fewer studies that have examined endurance effects or mechanisms explaining this possible effect, in addition to fewer studies including women or younger and older participants, and considering causal effects of prior training history through the use of longitudinal intervention study designs. Thus, it seems pertinent that future research on NLMF effects should be redirected towards these still relatively unexplored areas.
Involvement in sport and exercise not only provides participants with health benefits but can be an important aspect of living a meaningful life. The COVID-19 pandemic and the temporary cessation of public life in March/April/May 2020 came with restrictions, which probably also made it difficult, if not impossible, to participate in certain types of sport or exercise. Following the philosophical position that different types of sport and exercise offer different ways of "relating to the world," this study explored (dis)continuity in the type of sport and exercise people practiced during the pandemic-related lockdown, and possible effects on mood. Data from a survey of 601 adult exercisers, collected shortly after the COVID-19 outbreak in Finland, were analyzed. Approximately one third (35%) of the participants changed their "worldmaking" and shifted to "I-Nature"-type activities. We observed worse mood during the pandemic in those who shifted from "I-Me," compared to those who had preferred the "I-Nature" relation already before the pandemic and thus experienced continuity. The clouded mood of those experiencing discontinuity may be the result of a temporary loss of "feeling at home" in their new exercise life-world. However, further empirical investigation must follow, because the observed effect sizes were small.
Mining of metabolite-protein interaction networks facilitates the identification of design principles underlying the regulation of different cellular processes. However, identification and characterization of the regulatory role that metabolites play in interactions with proteins on a genome-scale level remains a pressing task. Based on availability of high-quality metabolite-protein interaction networks and genome-scale metabolic networks, here we propose a supervised machine learning approach, called CIRI that determines whether or not a metabolite is involved in a competitive inhibitory regulatory interaction with an enzyme. First, we show that CIRI outperforms the naive approach based on a structural similarity threshold for a putative competitive inhibitor and the substrates of a metabolic reaction. We also validate the performance of CIRI on several unseen data sets and databases of metabolite-protein interactions not used in the training, and demonstrate that the classifier can be effectively used to predict competitive inhibitory interactions. Finally, we show that CIRI can be employed to refine predictions about metabolite-protein interactions from a recently proposed PROMIS approach that employs metabolomics and proteomics profiles from size exclusion chromatography in E. coli to predict metaboliteprotein interactions. Altogether, CIRI fills a gap in cataloguing metabolite-protein interactions and can be used in directing future machine learning efforts to categorize the regulatory type of these interactions.
We have directly resolved in the present work the interfacial composition during and after the interactions of a saturated atmosphere of oil vapor with soluble surfactant solutions at a planar water/air interface for the first time. Experiments were conducted on interactions of hexane vapor with solutions of alkyltrimethylammonium bromides and sodium dodecyl sulfate to observe the balance between cooperativity and competition of the components at the interface.
In all cases, hexane adsorption was strongly enhanced by the presence of the surfactant, even at bulk surfactant concentrations four orders of magnitude below the critical micelle concentration. Cooperativity of the surfactant adsorption was observed only for sodium dodecyl sulfate at intermediate bulk concentrations, yet for all four systems, competition set in at higher concentrations, as hexane adsorption reduced the surfactant surface excess. The data fully supported the complete removal of hexane from the interface following venting of the system to remove the saturated atmosphere of oil vapor.
These results help to identify future experiments that would elaborate and could explain the cooperativity of surfactant adsorption, such as on cationic surfactants with short alkyl chains and a broader series of anionic surfactants. This work holds relevance for oil recovery applications with foam, where there is a gas phase saturated with oil vapor.
Phonologie, die Lehre von der lautlichen Organisation der Sprache, ist einer der zentralen Bereiche der Sprachwissenschaft. Auch das Schriftsystem, das Gegenstand der Graphematik ist, gewinnt zunehmend an Bedeutung für Forschung und Lehre. Dieser Band informiert über Form und Funktion der wichtigsten Einheiten des gesprochenen und geschriebenen Deutschen, von den Lauten und Buchstaben über Sprech- und Schreibsilben bis hin zu phonologischen und graphematischen Wörtern, phonologischen Äußerungen und graphematischen Sätzen. In zweifarbiger Gestaltung mit Definitionen, Beispielen, Grafiken und Aufgaben. – Die 2. Auflage wurde durchgängig aktualisiert und erweitert.