Refine
Has Fulltext
- no (4)
Document Type
- Article (4) (remove)
Language
- English (4)
Is part of the Bibliography
- yes (4)
Keywords
- FhuA (2)
- BBTP (1)
- Grafting-from polymerization (1)
- NIPAAm (1)
- Protein-polymer conjugate (1)
- Transmembrane protein (1)
- block copolymers (1)
- electrochemical impedance spectroscopy (1)
- enzymes (1)
- nanoparticles (1)
- polymersome spreading (1)
- solid-supported biomimetic membranes (1)
- sortase-mediated ligation (1)
- synthosomes (1)
Institute
- Institut für Chemie (4) (remove)
Sortase A (SrtA) from Staphylococcus aureus has been often used for ligating a protein with other natural or synthetic compounds in recent years. Here we show that SrtA-mediated ligation (SML) is universally applicable for the linkage of two purely artificial building blocks. Silica nanoparticles (NPs), poly(ethylene glycol) and poly(N-isopropyl acrylamide) are chosen as synthetic building blocks. As a proof of concept, NP-polymer, NP-NP, and polymer-polymer structures are formed by SrtA catalysis. Therefore, the building blocks are equipped with the recognition sequence needed for SrtA reaction-the conserved peptide LPETG-and a pentaglycine motif. The successful formation of the reaction products is shown by means of transmission electron microscopy (TEM), matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS), and dynamic light scattering (DLS). The sortase catalyzed linkage of artificial building blocks sets the stage for the development of a new approach to link synthetic structures in cases where their synthesis by established chemical methods is complicated.