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Monocytes and macrophages are key players in maintaining immune homeostasis. Identifying strategies to manipulate their functions via gene delivery is thus of great interest for immunological research and biomedical applications. We set out to establish conditions for mRNA transfection in hard-to-transfect primary human monocytes and monocyte-derived macrophages due to the great potential of gene expression from in vitro transcribed mRNA for modulating cell phenotypes. mRNA doses, nucleotide modifications, and different carriers were systematically explored in order to optimize high mRNA transfer rates while minimizing cell stress and immune activation. We selected three commercially available mRNA transfection reagents including liposome and polymer-based formulations, covering different application spectra. Our results demonstrate that liposomal reagents can particularly combine high gene transfer rates with only moderate immune cell activation. For the latter, use of specific nucleotide modifications proved essential. In addition to improving efficacy of gene transfer, our findings address discrete aspects of innate immune activation using cytokine and surface marker expression, as well as cell viability as key readouts to judge overall transfection efficiency. The impact of this study goes beyond optimizing transfection conditions for immune cells, by providing a framework for assessing new gene carrier systems for monocyte and macrophage, tailored to specific applications.
Magnetic fields play important roles in many astrophysical processes. However, there is no universal diagnostic for the magnetic fields in the interstellar medium (ISM) and each magnetic tracer has its limitation. Any new detection method is thus valuable. Theoretical studies have shown that submillimetre fine-structure lines are polarized due to atomic alignment by ultraviolet photon-excitation, which opens up a new avenue to probe interstellar magnetic fields. We will, for the first time, perform synthetic observations on the simulated three-dimensional ISM to demonstrate the measurability of the polarization of submillimetre atomic lines. The maximum polarization for different absorption and emission lines expected from various sources, including star-forming regions are provided. Our results demonstrate that the polarization of submillimetre atomic lines is a powerful magnetic tracer and add great value to the observational studies of the submilimetre astronomy.
We report the detection of the auroral radio emission from the early-type magnetic star HD142301. New VLA observations of HD142301 detected highly polarized amplified emission occurring at fixed stellar orientations. The coherent emission mechanism responsible for the stellar auroral radio emission amplifies the radiation within a narrow beam, making the star where this phenomenon occurs similar to a radio lighthouse. The elementary emission process responsible for the auroral radiation mainly amplifies one of the two magneto-ionic modes of the electromagnetic wave. This explains why the auroral pulses are highly circularly polarized. The auroral radio emission of HD142301 is characterized by a reversal of the sense of polarization as the star rotates. The effective magnetic field curve of HD142301 is also available making it possible to correlate the transition from the left to the right-hand circular polarization sense ( and vice versa) of the auroral pulses with the known orientation of the stellar magnetic field. The results presented in this letter have implications for the estimation of the dominant magneto-ionic mode amplified within the HD142301 magnetosphere.