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Microbial community composition and abundance after millennia of submarine permafrost warming
(2019)
Warming of the Arctic led to an increase in permafrost temperatures by about 0.3 degrees C during the last decade. Permafrost warming is associated with increasing sediment water content, permeability, and diffusivity and could in the long term alter microbial community composition and abundance even before permafrost thaws. We studied the long-term effect (up to 2500 years) of submarine permafrost warming on microbial communities along an onshore-offshore transect on the Siberian Arctic Shelf displaying a natural temperature gradient of more than 10 degrees C. We analysed the in situ development of bacterial abundance and community composition through total cell counts (TCCs), quantitative PCR of bacterial gene abundance, and amplicon sequencing and correlated the microbial community data with temperature, pore water chemistry, and sediment physicochemical parameters. On timescales of centuries, permafrost warming coincided with an overall decreasing microbial abundance, whereas millennia after warming microbial abundance was similar to cold onshore permafrost. In addition, the dissolved organic carbon content of all cores was lowest in submarine permafrost after millennial-scale warming. Based on correlation analysis, TCC, unlike bacterial gene abundance, showed a significant rank-based negative correlation with increasing temperature, while bacterial gene copy numbers showed a strong negative correlation with salinity. Bacterial community composition correlated only weakly with temperature but strongly with the pore water stable isotopes delta O-18 and delta D, as well as with depth. The bacterial community showed substantial spatial variation and an overall dominance of Actinobacteria, Chloroflexi, Firmicutes, Gemmatimonadetes, and Proteobacteria, which are amongst the microbial taxa that were also found to be active in other frozen permafrost environments. We suggest that, millennia after permafrost warming by over 10 degrees C, microbial community composition and abundance show some indications for proliferation but mainly reflect the sedimentation history and paleoenvironment and not a direct effect through warming.
The activity of neutral sphingomyelinase-2 (NSM2) to catalyze the conversion of sphingomyelin (SM) to ceramide and phosphocholine at the cytosolic leaflet of plasma membrane (PM) is important in T cell receptor (TCR) signaling. We recently identified PKC zeta as a major NSM2 downstream effector which regulates microtubular polarization. It remained, however, unclear to what extent NSM2 activity affected overall composition of PM lipids and downstream effector lipids in antigen stimulated T cells. Here, we provide a detailed lipidomics analyses on PM fractions isolated from TCR stimulated wild type and NSM2 deficient (Delta NSM) Jurkat T cells. This revealed that in addition to that of sphingolipids, NSM2 depletion also affected concentrations of many other lipids. In particular, NSM2 ablation resulted in increase of lyso-phosphatidylcholine (LPC) and lyso-phosphatidylethanolamine (LPE) which both govern PM biophysical properties. Crucially, TCR dependent upregulation of the important T cell signaling lipid diacylglycerol (DAG), which is fundamental for activation of conventional and novel PKCs, was abolished in Delta NSM cells. Moreover, NSM2 activity was found to play an important role in PM cholesterol transport to the endoplasmic reticulum (ER) and production of cholesteryl esters (CE) there. Most importantly, CE accumulation was essential to sustain human T cell proliferation. Accordingly, inhibition of CE generating enzymes, the cholesterol acetyltransferases ACAT1/SOAT1 and ACAT2/SOAT2, impaired TCR driven expansion of both CD4(+) and CD8(+) T cells. In summary, our study reveals an important role of NSM2 in regulating T cell functions by its multiple effects on PM lipids and cholesterol homeostasis.
Permafrost is warming in the northern high latitudes, inducing highly dynamic thaw-related permafrost disturbances across the terrestrial Arctic. Monitoring and tracking of permafrost disturbances is important as they impact surrounding landscapes, ecosystems and infrastructure. Remote sensing provides the means to detect, map, and quantify these changes homogeneously across large regions and time scales. Existing Landsat-based algorithms assess different types of disturbances with similar spatiotemporal requirements. However, Landsat-based analyses are restricted in northern high latitudes due to the long repeat interval and frequent clouds, in particular at Arctic coastal sites. We therefore propose to combine Landsat and Sentinel-2 data for enhanced data coverage and present a combined annual mosaic workflow, expanding currently available algorithms, such as LandTrendr, to achieve more reliable time series analysis. We exemplary test the workflow for twelve sites across the northern high latitudes in Siberia. We assessed the number of images and cloud-free pixels, the spatial mosaic coverage and the mosaic quality with spectral comparisons. The number of available images increased steadily from 1999 to 2019 but especially from 2016 onward with the addition of Sentinel-2 images. Consequently, we have an increased number of cloud-free pixels even under challenging environmental conditions, which then serve as the input to the mosaicking process. In a comparison of annual mosaics, the Landsat+Sentinel-2 mosaics always fully covered the study areas (99.9–100 %), while Landsat-only mosaics contained data-gaps in the same years, only reaching coverage percentages of 27.2 %, 58.1 %, and 69.7 % for Sobo Sise, East Taymyr, and Kurungnakh in 2017, respectively. The spectral comparison of Landsat image, Sentinel-2 image, and Landsat+Sentinel-2 mosaic showed high correlation between the input images and mosaic bands (e.g., for Kurungnakh 0.91–0.97 between Landsat and Landsat+Sentinel-2 mosaic and 0.92–0.98 between Sentinel-2 and Landsat+Sentinel-2 mosaic) across all twelve study sites, testifying good quality mosaic results. Our results show that especially the results for northern, coastal areas was substantially improved with the Landsat+Sentinel-2 mosaics. By combining Landsat and Sentinel-2 data we accomplished to create reliably high spatial resolution input mosaics for time series analyses. Our approach allows to apply a high temporal continuous time series analysis to northern high latitude permafrost regions for the first time, overcoming substantial data gaps, and assess permafrost disturbance dynamics on an annual scale across large regions with algorithms such as LandTrendr by deriving the location, timing and progression of permafrost thaw disturbances
(1) Background: People with HIV (PWH) may perform more than one type of exercise cumulatively. The objective of this study is to investigate recreational exercise and its association with health-related quality of life (HRQOL) and comorbidities in relation to potential covariates. (2) Methods: The HIBES study (HIV-Begleiterkrankungen-Sport) is a cross-sectional study for people with HIV. The differences between non-exercisers versus exercisers (cumulated vs. single type of exercises) were investigated using regression models based on 454 participants. (3) Results: Exercisers showed a higher HRQOL score compared to non-exercisers (Wilcox r = 0.2 to 0.239). Psychological disorders were identified as the main covariate. Participants performing exercise cumulatively showed higher scores in duration, frequency, and intensity when compared to participants performing only one type of exercise. The mental health summary score was higher for the cumulated and single type of exercise if a psychological disorder existed. Duration and intensity were associated with an increase of HRQOL, whilst a stronger association between psychological disorders and exercise variables were evident. Exercise duration (minutes) showed a significant effect on QOL (standardized beta = 0.1) and for participants with psychological disorders (standardized beta = 0.3), respectively. (4) Conclusions: Psychological disorders and other covariates have a prominent effect on HRQOL and its association with exercise. For PWH with a psychological disorder, a stronger relationship between HRQOL with exercise duration and intensity emerged. However, differentiation of high-HRQOL individuals warrants further investigation by considering additional factors.
The aim of this study was to establish maturation-, age-, and sex-specific anthropometric and physical fitness percentile reference values of young elite athletes from various sports. Anthropometric (i.e., standing and sitting body height, body mass, body mass index) and physical fitness (i.e., countermovement jump, drop jump, change-of-direction speed [i.e., T-test], trunk muscle endurance [i.e., ventral Bourban test], dynamic lower limbs balance [i.e., Y-balance test], hand grip strength) of 703 male and female elite young athletes aged 8–18 years were collected to aggregate reference values according to maturation, age, and sex. Findings indicate that body height and mass were significantly higher (p<0.001; 0.95≤d≤1.74) in more compared to less mature young athletes as well as with increasing chronological age (p<0.05; 0.66≤d≤3.13). Furthermore, male young athletes were significantly taller and heavier compared to their female counterparts (p<0.001; 0.34≤d≤0.50). In terms of physical fitness, post-pubertal athletes showed better countermovement jump, drop jump, change-of-direction, and handgrip strength performances (p<0.001; 1.57≤d≤8.72) compared to pubertal athletes. Further, countermovement jump, drop jump, change-of-direction, and handgrip strength performances increased with increasing chronological age (p<0.05; 0.29≤d≤4.13). In addition, male athletes outperformed their female counterpart in the countermovement jump, drop jump, change-of-direction, and handgrip strength (p<0.05; 0.17≤d≤0.76). Significant age by sex interactions indicate that sex-specific differences were even more pronounced with increasing age. Conclusively, body height, body mass, and physical fitness increased with increasing maturational status and chronological age. Sex-specific differences appear to be larger as youth grow older. Practitioners can use the percentile values as approximate benchmarks for talent identification and development.
Uplift in the broken Andean foreland of the Argentine Santa Bárbara System (SBS) is associated with the contractional reactivation of basement anisotropies, similar to those reported from the thick-skinned Cretaceous-Eocene Laramide province of North America. Fault scarps, deformed Quaternary deposits and landforms, disrupted drainage patterns, and medium-sized earthquakes within the SBS suggest that movement along these structures may be a recurring phenomenon, with yet to be defined repeat intervals and rupture lengths. In contrast to the Subandes thrust belt farther north, where eastward-migrating deformation has generated a well-defined thrust front, the SBS records spatiotemporally disparate deformation along structures that are only known to the first order. We present herein the results of geomorphic desktop analyses, structural field observations, and 2D electrical resistivity tomography and seismic-refraction tomography surveys and an interpretation of seismic reflection profiles across suspected fault scarps in the sedimentary basins adjacent to the Candelaria Range (CR) basement uplift, in the south-central part of the SBS. Our analysis in the CR piedmont areas reveals consistency between the results of near-surface electrical resistivity and seismic-refraction tomography surveys, the locations of prominent fault scarps, and structural geometries at greater depth imaged by seismic reflection data. We suggest that this deformation is driven by deep-seated blind thrusting beneath the CR and associated regional warping, while shortening involving Mesozoic and Cenozoic sedimentary strata in the adjacent basins was accommodated by layer-parallel folding and flexural-slip faults that cut through Quaternary landforms and deposits at the surface.
(1) Environmental conditions experienced in the past may lead to intraspecific differences in ecological and chemical traits of plants, which likely affect future responses to altered or new environments. Whether competition by neighbors is such a trait-shaping factor is not yet well-known. We aimed to understand how the level of ancestral plant competition affects traits related to plant fitness and resource allocation, reproduction, and (phyto-)toxin accumulation in offspring, and whether a potential differentiation in these traits can be found in different geographic origins of which one belongs to the native and one to the invaded range. (2) We compared differentiation of the following traits in offspring plants of multiple populations in Erodium cicutarium (Geraniaceae): biomass, seed production, seed traits related to dispersal and germination, and concentrations of foliar mono- and sesquiterpenes. We tested the allelopatic potential of aqueous extracts of the same E. cicutarium plants on seeds of five different plant families. (3) In plants originating from populations that experienced high levels of competition, we found twice as high monoterpene concentrations. These plants also produced more biomass and a higher proportion of ripe to unripe seeds until harvesting. Seeds originating from high competition sites were shorter. Aqueous E. cicutarium leaf extracts with high terpenoid content reduced radicle length of Zea mays and radicle and hypocotyl length of E. cicutarium seedlings. (4) The results of this study provide first evidence that the surrounding vegetation may shape chemo-ecological plant traits that may be fundamental for competitive ability. Our study calls for more research testing whether competition experienced in the native range may lead to an enhanced capability of plants to establish populations and spread in a new range.
Of city and village mice
(2020)
A fundamental question of current ecological research concerns the drives and limits of species responses to human-induced rapid environmental change (HIREC). Behavioural responses to HIREC are a key component because behaviour links individual responses to population and community changes. Ongoing fast urbanization provides an ideal setting to test the functional role of behaviour for responses to HIREC. Consistent behavioural differences between conspecifics (animal personality) may be important determinants or constraints of animals’ adaptation to urban habitats. We tested whether urban and rural populations of small mammals differ in mean trait expression, flexibility and repeatability of behaviours associated to risk-taking and exploratory tendencies. Using a standardized behavioural test in the field, we quantified spatial exploration and boldness of striped field mice (Apodemus agrarius, n = 96) from nine sub-populations, presenting different levels of urbanisation and anthropogenic disturbance. The level of urbanisation positively correlated with boldness, spatial exploration and behavioural flexibility, with urban dwellers being bolder, more explorative and more flexible in some traits than rural conspecifics. Thus, individuals seem to distribute in a non-random way in response to human disturbance based on their behavioural characteristics. Animal personality might therefore play a key role in successful coping with the challenges of HIREC.
The passive and active motion of micron-sized tracer particles in crowded liquids and inside living biological cells is ubiquitously characterised by 'viscoelastic' anomalous diffusion, in which the increments of the motion feature long-ranged negative and positive correlations. While viscoelastic anomalous diffusion is typically modelled by a Gaussian process with correlated increments, so-called fractional Gaussian noise, an increasing number of systems are reported, in which viscoelastic anomalous diffusion is paired with non-Gaussian displacement distributions. Following recent advances in Brownian yet non-Gaussian diffusion we here introduce and discuss several possible versions of random-diffusivity models with long-ranged correlations. While all these models show a crossover from non-Gaussian to Gaussian distributions beyond some correlation time, their mean squared displacements exhibit strikingly different behaviours: depending on the model crossovers from anomalous to normal diffusion are observed, as well as a priori unexpected dependencies of the effective diffusion coefficient on the correlation exponent. Our observations of the non-universality of random-diffusivity viscoelastic anomalous diffusion are important for the analysis of experiments and a better understanding of the physical origins of 'viscoelastic yet non-Gaussian' diffusion.
We consider the emerging dynamics of a separable continuous time random walk (CTRW) in the case when the random walker is biased by a velocity field in a uniformly growing domain. Concrete examples for such domains include growing biological cells or lipid vesicles, biofilms and tissues, but also macroscopic systems such as expanding aquifers during rainy periods, or the expanding Universe. The CTRW in this study can be subdiffusive, normal diffusive or superdiffusive, including the particular case of a Lévy flight. We first consider the case when the velocity field is absent. In the subdiffusive case, we reveal an interesting time dependence of the kurtosis of the particle probability density function. In particular, for a suitable parameter choice, we find that the propagator, which is fat tailed at short times, may cross over to a Gaussian-like propagator. We subsequently incorporate the effect of the velocity field and derive a bi-fractional diffusion-advection equation encoding the time evolution of the particle distribution. We apply this equation to study the mixing kinetics of two diffusing pulses, whose peaks move towards each other under the action of velocity fields acting in opposite directions. This deterministic motion of the peaks, together with the diffusive spreading of each pulse, tends to increase particle mixing, thereby counteracting the peak separation induced by the domain growth. As a result of this competition, different regimes of mixing arise. In the case of Lévy flights, apart from the non-mixing regime, one has two different mixing regimes in the long-time limit, depending on the exact parameter choice: in one of these regimes, mixing is mainly driven by diffusive spreading, while in the other mixing is controlled by the velocity fields acting on each pulse. Possible implications for encounter–controlled reactions in real systems are discussed.
Empirical evidence of the relationship between social support and post-disaster mental health provides support for a general beneficial effect of social support (main-effect model; Wheaton, 1985). From a theoretical perspective, a buffering effect of social support on the negative relationship between disaster-related stress and mental health also seems plausible (stress-buffering model; Wheaton, 1985). Previous studies, however, (a) have paid less attention to the buffering effect of social support and (b) have mainly relied on interpersonal support (but not collective-level support such as community resilience) when investigating this issue. This previous work might have underestimated the effect of support on post-disaster mental health. Building on a sample of residents in Germany recently affected by flooding (N = 118), we show that community resilience to flooding (but not general interpersonal social support) buffered against the negative effects of flooding on post-disaster mental health. The results support the stress-buffering model and call for a more detailed look at the relationship between support and resilience and post-disaster adjustment, including collective-level variables.
Background: The regular assessment of hormonal and mood state parameters in professional soccer are proposed as good indicators during periods of intense training and/or competition to avoid overtraining.
Objective: The aim of this study was to analyze hormonal, psychological, workload and physical fitness parameters in elite soccer players in relation to changes in training and match exposure during a congested period of match play.
Methods: Sixteen elite soccer players from a team playing in the first Tunisian soccer league were evaluated three times (T1, T2, and T3) over 12 weeks. The non-congested period of match play was from T1 to T2, when the players played 6 games over 6 weeks. The congested period was from T2 to T3, when the players played 10 games over 6 weeks. From T1 to T3, players performed the Yo-Yo intermittent recovery test level 1 (YYIR1), the repeated shuttle sprint ability test (RSSA), the countermovement jump test (CMJ), and the squat jump test (SJ). Plasma Cortisol (C), Testosterone (T), and the T/C ratio were analyzed at T1, T2, and T3. Players had their mood dimensions (tension, depression, anger, vigor, fatigue, confusion, and a Total Mood Disturbance) assessed through the Profile of Mood State questionnaire (POMS). Training session rating of perceived exertion (sRPE) was also recorded on a daily basis in order to quantify internal training load and elements of monotony and strain.
Results: Significant performance declines (T1 < T2 < T3) were found for SJ performance (p = 0.04, effect size [ES] ES₁₋₂ = 0.15−0.06, ES₂₋₃ = 0.24) from T1 to T3. YYIR1 performance improved significantly from T1 to T2 and declined significantly from T2 to T3 (p = 0.001, ES₁₋₂ = 0.24, ES₂₋₃ = −2.54). Mean RSSA performance was significantly higher (p = 0.019, ES₁₋₂ = −0.47, ES₂₋₃ = 1.15) in T3 compared with T2 and T1. Best RSSA performance was significantly higher in T3 when compared with T2 and T1 (p = 0.006, ES₂₋₃ = 0.47, ES₁₋₂ = −0.56), but significantly lower in T2 when compared with to T1. T and T/C were significantly lower in T3 when compared with T2 and T1 (T: p = 0.03, ES₃₋₂ = −0.51, ES₃₋₁ = −0.51, T/C: p = 0.017, ES₃₋₂ = −1.1, ES₃₋₁ = −1.07). Significant decreases were found for the vigor scores in T3 when compared to T2 and T1 (p = 0.002, ES₁₋₂ = 0.31, ES₃₋₂ = −1.25). A significant increase was found in fatigue scores in T3 as compared to T1 and T2 (p = 0.002, ES₁₋₂ = 0.43, ES₂₋₃ = 0.81). A significant increase was found from T1 < T2 < T3 intension score (p = 0.002, ES₁₋₂ = 1.1, ES₂₋₃ = 0.2) and anger score (p = 0.03, ES₁₋₂ = 0.47, ES₂₋₃ = 0.33) over the study period. Total mood disturbance increased significantly (p = 0.02, ES₁₋₂ = 0.91, ES₂₋₃ = 1.1) from T1 to T3. Between T1-T2, significant relationships were observed between workload and changes in T (r = 0.66, p = 0.003), and T/C ratio (r = 0.62, p = 0.01). There were significant relationships between performance in RSSAbest and training load parameters (workload: r = 0.52, p = 0.03; monotony: r = 0.62, p = 0.01; strain: r = 0.62, p = 0.009). Between T2-T3, there was a significant relationship between Δ% of total mood disturbance and Δ% of YYIR1 (r = −0.54; p = 0.04), RSSAbest (r = 0.58, p = 0.01), SJ (r = −0,55, p = 0.01), T (r = 0.53; p = 0.03), and T/C (r = 0.5; p = 0.04).
Conclusion: An intensive period of congested match play significantly compromised elite soccer players’ physical and mental fitness. These changes were related to psychological but not hormonal parameters; even though significant alterations were detected for selected measures. Mood monitoring could be a simple and useful tool to determine the degree of preparedness for match play during a congested period in professional soccer.
The great auk was once abundant and distributed across the North Atlantic. It is now extinct, having been heavily exploited for its eggs, meat, and feathers. We investigated the impact of human hunting on its demise by integrating genetic data, GPS-based ocean current data, and analyses of population viability. We sequenced complete mitochondrial genomes of 41 individuals from across the species' geographic range and reconstructed population structure and population dynamics throughout the Holocene. Taken together, our data do not provide any evidence that great auks were at risk of extinction prior to the onset of intensive human hunting in the early 16th century. In addition, our population viability analyses reveal that even if the great auk had not been under threat by environmental change, human hunting alone could have been sufficient to cause its extinction. Our results emphasise the vulnerability of even abundant and widespread species to intense and localised exploitation.
Verwaltungsmodernisierung
(2020)
Die Modernisierung der öffentlichen Verwaltung hat sich längst zu einer Daueraufgabe entwickelt. In den Modernisierungsprozessen stehen derzeit zwei Segmente besonders im Fokus: Digitalisierung und Partizipation. Digitalisierung geht heute weit über die ursprünglich mit diesem Stichwort angesprochene Umwandlung analoger Daten in elektronische Datenformate hinaus. Die Programmatik der Digitalisierung zielt auf den möglichst umfassenden Einsatz elektronischer Informations- und Kommunikationstechniken zur Unterstützung, Beschleunigung, Rationalisierung und in Teilen auch Ersetzung von herkömmlichen Verwaltungsabläufen mit der Perspektive auf eine Transformation und Durchdringung sowohl des gesamten Verwaltungshandelns als auch der gesamten Verwaltungsorganisation. Demgegenüber meint Partizipation nach gängigem Verständnis die Beteiligung von Bürgern und anderen gesellschaftlichen Akteuren an politischen Willensbildungs- und Entscheidungsprozessen. In spezifisch administrativem Zusammenhang geht es vor allem um die Einbindung von Bürgern in Entscheidungsprozesse der Verwaltung und um die Aktivierung der Zivilgesellschaft, die Erschließung bürgerschaftlichen Engagements sowie gesellschaftlicher Ressourcen für die Erfüllung von Verwaltungsaufgaben – und dies alles mit dem Ziel einer Verbesserung der Legitimität und Qualität öffentlicher Verwaltung. Die beiden Modernisierungsansätze stehen nicht unverbunden nebeneinander. Vielmehr gehen sie nicht selten ineinander über. Beispiele dafür sind die Bereitstellung von Optionen zu elektronischer Stellung und Bearbeitung von Anträgen sowie von elektronischen Plattformen für die Bürgerpartizipation.
An diesen Themenfeldern der Verwaltungsmodernisierung setzt der Tagungsband an. Er dokumentiert den Gedankenaustausch über Digitalisierungs- und Partizipationsprozesse zwischen polnischen und deutschen Verwaltungsrechtswissenschaftlern auf einer Tagung, die im Dezember 2019 an der Universität Potsdam stattgefunden hat.
Narratives are shaping our understanding of the world. They convey values and norms and point to desirable future developments. In this way, they justify and legitimize political actions and social practices. Once a narrative has emerged and this world view is supported by broad societal groups, narratives can provide powerful momentum to trigger innovation and changes in the course of action. Narratives, however, are not necessarily based on evidence and precise categories, but can instead be vague and ambiguous in order to be acceptable and attractive to different actors. However, the more open and inclusive a narrative is, the less impact can be expected. We investigate whether there is a shared narrative in research for the sustainable economy and how this can be evaluated in terms of its potential societal impact. The paper carves out the visions for the future that have been underlying the research projects conducted within the German Federal Ministry of Education and Research (BMBF) funding programme "The Sustainable Economy". It then analyzes whether these visions are compatible with narratives dominating societal discourse on the sustainable economy, and concludes how the use of visions and narratives in research can contribute to fostering societal transformations.
Plasticity in metabolism underpins local responses to nitrogen in Arabidopsis thaliana populations
(2019)
Nitrogen (N) is central for plant growth, and metabolic plasticity can provide a strategy to respond to changing N availability. We showed that two local A. thaliana populations exhibited differential plasticity in the compounds of photorespiratory and starch degradation pathways in response to three N conditions. Association of metabolite levels with growth-related and fitness traits indicated that controlled plasticity in these pathways could contribute to local adaptation and play a role in plant evolution.
During the last few decades, the rapid separation of the Small Aral Sea from the isolated basin has changed its hydrological and ecological conditions tremendously. In the present study, we developed and validated the hybrid model for the Syr Darya River basin based on a combination of state-of-the-art hydrological and machine learning models. Climate change impact on freshwater inflow into the Small Aral Sea for the projection period 2007-2099 has been quantified based on the developed hybrid model and bias corrected and downscaled meteorological projections simulated by four General Circulation Models (GCM) for each of three Representative Concentration Pathway scenarios (RCP). The developed hybrid model reliably simulates freshwater inflow for the historical period with a Nash-Sutcliffe efficiency of 0.72 and a Kling-Gupta efficiency of 0.77. Results of the climate change impact assessment showed that the freshwater inflow projections produced by different GCMs are misleading by providing contradictory results for the projection period. However, we identified that the relative runoff changes are expected to be more pronounced in the case of more aggressive RCP scenarios. The simulated projections of freshwater inflow provide a basis for further assessment of climate change impacts on hydrological and ecological conditions of the Small Aral Sea in the 21st Century.
Recombinant adeno-associated viruses (rAAV) provide outstanding options for customization and superior capabilities for gene therapy. To access their full potential, facile genetic manipulation is pivotal, including capsid loop modifications. Therefore, we assessed capsid tolerance to modifications of the structural VP proteins in terms of stability and plasticity. Flexible glycine-serine linkers of increasing sizes were, at the genetic level, introduced into the 587 loop region of the VP proteins of serotype 2, the best studied AAV representative. Analyses of biological function and thermal stability with respect to genome release of viral particles revealed structural plasticity. In addition, insertion of the 29 kDa enzyme beta-lactamase into the loop region was tested with a complete or a mosaic modification setting. For the mosaic approach, investigation of VP2 trans expression revealed that a Kozak sequence was required to prevent leaky scanning. Surprisingly, even the full capsid modification with beta-lactamase allowed for the assembly of capsids with a concomitant increase in size. Enzyme activity assays revealed lactamase functionality for both rAAV variants, which demonstrates the structural robustness of this platform technology.
Colombia's agriculture, forestry and other land use sector accounts for nearly half of its total greenhouse gas (GHG) emissions. The importance of smallholder deforestation is comparatively high in relation to its regional counterparts, and livestock agriculture represents the largest driver of primary forest depletion. Silvopastoral systems (SPSs) are presented as agroecological solutions that synergistically enhance livestock productivity, improve local farmers' livelihoods and hold the potential to reduce pressure on forest conversion. The department of Caquetá represents Colombia's most important deforestation hotspot. Targeting smallholder livestock farms through survey data, in this work we investigate the GHG mitigation potential of implementing SPSs for smallholder farms in this region. Specifically, we assess whether the carbon sequestration taking place in the soil and biomass of SPSs is sufficient to offset the per-hectare increase in livestock GHG emissions resulting from higher stocking rates. To address these questions we use data on livestock population characteristics and historic land cover changes reported from a survey covering 158 farms and model the carbon sequestration occurring in three different scenarios of progressively-increased SPS complexity using the CO2 fix model. We find that, even with moderate tree planting densities, the implementation of SPSs can reduce GHG emissions by 2.6 Mg CO2e ha−1 yr−1 in relation to current practices, while increasing agriculture productivity and contributing to the restoration of severely degraded landscapes.