Refine
Has Fulltext
- no (13)
Year of publication
Document Type
- Article (13) (remove)
Language
- English (13) (remove)
Is part of the Bibliography
- yes (13)
Keywords
- Electrospray Ionization (1)
- Fabry-Perot etalon (1)
- Fluorescence (1)
- Gaseous Ions (1)
- High-resolution spectrometer (1)
- LIBS (1)
- Laser-induced breakdown spectroscopy (1)
- Lucigenin (1)
- Raman spectroscopy (1)
- Rhodamine 6G (1)
Institute
pH sensing in living cells represents one of the most prominent topics in biochemistry and physiology. In this study we performed one-photon and two-photon time-domain fluorescence lifetime imaging with a laser-scanning microscope using the time-correlated single-photon counting technique for imaging intracellular pH levels. The suitability of different commercial fluorescence dyes for lifetime-based pH sensing is discussed on the basis of in vitro as well of in situ measurements. Although the tested dyes are suitable for intensity-based ratiometric measurements, for lifetime- based techniques in the time-domain so far only BCECF seems to meet the requirements of reliable intracellular pH recordings in living cells.