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Grunzel, Petra ; Pilarek, Maciej ; Steinbrueck, Doerte ; Neubauer, Antje ; Brand, Eva ; Kumke, Michael Uwe ; Neubauer, Peter ; Krause, Mirja
The standard procedure in the lab for plasmid isolation usually involves a 2-mL, 16 h over-night cultivation in 15-mL bioreaction tubes in LB medium. This is time consuming, and not suitable for high-throughput applications. This study shows that it is possible to produce plasmid DNA (pDNA) in a 1.5-mL microcentrifuge tube with only 100 L cultivation volume in less than 7 h with a simple protocol. Compared with the standard LB cultivation for pDNA production reaching a final pDNA concentration range of 1.5-4 mu g mL(-1), a 6- to 10-fold increase in plasmid concentration (from 10 up to 25 mu g mL(-1) cultivation volume) is achieved using an optimized medium with an internal substrate delivery system (EnBase (R)). Different strains, plasmids, and the applicability of different inoculation tools (i.e. different starting ODs) were compared, demonstrating the robustness of the system. Additionally, dissolved oxygen was monitored in real time online, indicating that under optimized conditions oxygen limitation can be avoided. We developed a simple protocol with a significantly decreased procedure time, enabling simultaneous handling of more samples, while a consistent quality and a higher final pDNA concentration are ensured.
Social media and self-esteem
(2022)
The relationship between social media and self-esteem is complex, as studies tend to find a mixed pattern of relationships and meta-analyses tend to find small, albeit significant, magnitudes of statistical effects. One explanation is that social media use does not affect self-esteem for the majority of users, while small minorities experience either positive or negative effects, as evidenced by recent research calculating person specific within-person effects. This suggests that the true relationship between social media use and self-esteem is person-specific and based on individual susceptibilities and uses. In recognition of these advancements, we review recent empirical studies considering differential uses and moderating variables in the social media-self-esteem relationship, and conclude by discussing opportunities for future social media effects research.
Stadie, Nicole ; Ulrich, Tanja ; Glück, Christian W. ; Richter, Kerstin ; Klassert, Annegret ; Krause, Carina Denise ; Wagner, Susanne ; Holzgrefe-Lang, Julia ; Lorenz, Elisa ; Oelze, Vera ; Schütz, Vivien ; Peinhardt, Ulrich ; Laßmann, Inga ; Hanne, Sandra ; Wollenberg, Maxi ; Düring, Sarah ; Laubscheer, Ann-Katrin ; Heide, Judith ; Gruhn, Sophie ; Segers, Eliane ; Keuning, Jos ; Verhoeven, Ludo ; Obry, Svenja ; Bohn, Bianca ; Neise, Romy ; Pregla, Dorothea ; Wiehe, Lea ; Weiland, Katharina ; Wahl, Michael
Das 13. Herbsttreffen Patholinguistik mit dem Schwerpunktthema »Nur ein Wort? Diagnostik und Therapie von Wortabrufstörungen bei Kindern und Erwachsenen« fand am 16.11.2019 in Potsdam statt. Das Herbsttreffen wird seit 2007 jährlich vom Verband für Patholinguistik e.V. (vpl) in Kooperation mit dem Deutschen Bundesverband für akademische Sprachtherapie und Logopädie (dbs) und der Universität Potsdam durchgeführt. Der vorliegende Tagungsband beinhaltet die Hauptvorträge zum Schwerpunktthema sowie die Beiträge der Kurzvorträge im »Spektrum Patholinguistik« und der Posterpräsentationen zu weiteren Themen aus der sprachtherapeutischen Forschung und Praxis.
The Late Permian Zechstein Group in northeastern Germany is characterized by shelf and slope carbonates that rimmed a basin extending from eastern England through the Netherlands and Germany to Poland. Conventional reservoirs are found in grainstones rimming islands created by pre-existing paleohighs and platform-rimming shoals that compose steep margins in the north and ramp deposits in the southern part. The slope and basin deposits are characterized by debris flows and organic-rich mudstones. Lagoonal and basinal evaporites formed the seal for these carbonate and underlying sandstone reservoirs. The objective of this investigation is to evaluate potential unconventional reservoirs in organic-rich, fine-grained and/or tight mudrocks in slope and basin as well as platform carbonates occurring in this stratigraphic interval. Therefore, a comprehensive study was conducted that included sedimentology, sequence stratigraphy, petrography, and geochemistry. Sequence stratigraphic correlations from shelf to basin are crucial in establishing a framework that allows correlation of potential productive facies in fine-grained, organic-rich basinal siliceous and calcareous mudstones or interfingering tight carbonates and siltstones, ranging from the lagoon, to slope to basin, which might be candidates for forming an unconventional reservoir. Most organic-rich shales worldwide are associated with eustatic transgressions. The basal Zechstein cycles, Z1 and Z2, contain organic-rich siliceous and calcareous mudstones and carbonates that form major transgressive deposits in the basin. Maturities range from over-mature (gas) in the basin to oil-generation on the slope with variable TOC contents. This sequence stratigraphic and sedimentologic evaluation of the transgressive facies in the Z1 and Z2 assesses the potential for shale-gas/oil and hybrid unconventional plays. Potential unconventional reservoirs might be explored in laminated organic-rich mudstones within the oil window along the northern and southern slopes of the basin. Although the Zechstein Z1 and Z2 cycles might have limited shale-gas potential because of low thickness and deep burial depth to be economic at this point, unconventional reservoir opportunities that include hybrid and shale-oil potential are possible in the study area.
Gohlke, Sabrina ; Zagoriy, Vyacheslav ; Inostroza, Alvaro Cuadros ; Meret, Michael ; Mancini, Carola ; Japtok, Lukasz ; Schumacher, Fabian ; Kuhlow, Doreen ; Graja, Antonia ; Stephanowitz, Heike ; Jähnert, Markus ; Krause, Eberhard ; Wernitz, Andreas ; Petzke, Klaus-Juergen ; Schürmann, Annette ; Kleuser, Burkhard ; Schulz, Tim Julius
Objective: Aging is accompanied by loss of brown adipocytes and a decline in their thermogenic potential, which may exacerbate the development of adiposity and other metabolic disorders. Presently, only limited evidence exists describing the molecular alterations leading to impaired brown adipogenesis with aging and the contribution of these processes to changes of systemic energy metabolism.
Methods: Samples of young and aged murine brown and white adipose tissue were used to compare age-related changes of brown adipogenic gene expression and thermogenesis-related lipid mobilization. To identify potential markers of brown adipose tissue aging, non-targeted proteomic and metabolomic as well as targeted lipid analyses were conducted on young and aged tissue samples. Subsequently, the effects of several candidate lipid classes on brown adipocyte function were examined.
Results: Corroborating previous reports of reduced expression of uncoupling protein-1, we observe impaired signaling required for lipid mobilization in aged brown fat after adrenergic stimulation. Omics analyses additionally confirm the age-related impairment of lipid homeostasis and reveal the accumulation of specific lipid classes, including certain sphingolipids, ceramides, and dolichols in aged brown fat. While ceramides as well as enzymes of dolichol metabolism inhibit brown adipogenesis, inhibition of sphingosine 1-phosphate receptor 2 induces brown adipocyte differentiation.
Conclusions: Our functional analyses show that changes in specific lipid species, as observed during aging, may contribute to reduced thermogenic potential. They thus uncover potential biomarkers of aging as well as molecular mechanisms that could contribute to the degradation of brown adipocytes, thereby providing potential treatment strategies of age-related metabolic conditions.
Volk, Benno ; Markert, Doreen ; Riejok, Henriette ; Dittberner, Jürgen ; Wanka, Johanna ; Wilkens, Martin ; Görtemaker, Manfred ; Regierer, Babette ; Steup, Martin ; Müller-Röber, Bernd ; Wernicke, Matthias ; Altenberger, Uwe ; Stölting, Erhard ; Ferý, Carolin ; Egenter, Peter ; Lenz, Claudia ; Jakubowski, Zuzanna ; Klötzer, Sylvia ; Krause, Michael ; Dietsch, Ulrich
Mettler, Tabea ; Mühlhaus, Timo ; Hemme, Dorothea ; Schöttler, Mark Aurel ; Rupprecht, Jens ; Idoine, Adam ; Veyel, Daniel ; Pal, Sunil Kumar ; Yaneva-Roder, Liliya ; Winck, Flavia Vischi ; Sommer, Frederik ; Vosloh, Daniel ; Seiwert, Bettina ; Erban, Alexander ; Burgos, Asdrubal ; Arvidsson, Samuel Janne ; Schoenfelder, Stephanie ; Arnold, Anne ; Guenther, Manuela ; Krause, Ursula ; Lohse, Marc ; Kopka, Joachim ; Nikoloski, Zoran ; Müller-Röber, Bernd ; Willmitzer, Lothar ; Bock, Ralph ; Schroda, Michael ; Stitt, Mark
We investigated the systems response of metabolism and growth after an increase in irradiance in the nonsaturating range in the algal model Chlamydomonas reinhardtii. In a three-step process, photosynthesis and the levels of metabolites increased immediately, growth increased after 10 to 15 min, and transcript and protein abundance responded by 40 and 120 to 240 min, respectively. In the first phase, starch and metabolites provided a transient buffer for carbon until growth increased. This uncouples photosynthesis from growth in a fluctuating light environment. In the first and second phases, rising metabolite levels and increased polysome loading drove an increase in fluxes. Most Calvin-Benson cycle (CBC) enzymes were substrate-limited in vivo, and strikingly, many were present at higher concentrations than their substrates, explaining how rising metabolite levels stimulate CBC flux. Rubisco, fructose-1,6-biosphosphatase, and seduheptulose-1,7-bisphosphatase were close to substrate saturation in vivo, and flux was increased by posttranslational activation. In the third phase, changes in abundance of particular proteins, including increases in plastidial ATP synthase and some CBC enzymes, relieved potential bottlenecks and readjusted protein allocation between different processes. Despite reasonable overall agreement between changes in transcript and protein abundance (R-2 = 0.24), many proteins, including those in photosynthesis, changed independently of transcript abundance.
Nordheim, J. ; Rapp, Michael A. ; Krause-Koehler, Kathleen ; Niemann-Mirmehdi, M. ; Haeusler, Andreas
The 2010 very high energy gamma-ray flare and 10 years ofmulti-wavelength oservations of M 87
(2012)
Abramowski, Attila ; Acero, F. ; Aharonian, Felix A. ; Akhperjanian, A. G. ; Anton, Gisela ; Balzer, Arnim ; Barnacka, Anna ; de Almeida, U. Barres ; Becherini, Yvonne ; Becker, J. ; Behera, B. ; Bernlöhr, K. ; Birsin, E. ; Biteau, Jonathan ; Bochow, A. ; Boisson, Catherine ; Bolmont, J. ; Bordas, Pol ; Brucker, J. ; Brun, Francois ; Brun, Pierre ; Bulik, Tomasz ; Buesching, I. ; Carrigan, Svenja ; Casanova, Sabrina ; Cerruti, M. ; Chadwick, Paula M. ; Charbonnier, A. ; Chaves, Ryan C. G. ; Cheesebrough, A. ; Clapson, A. C. ; Coignet, G. ; Cologna, Gabriele ; Conrad, Jan ; Dalton, M. ; Daniel, M. K. ; Davids, I. D. ; Degrange, B. ; Deil, C. ; Dickinson, H. J. ; Djannati-Ataï, A. ; Domainko, W. ; Drury, L. O'C. ; Dubus, G. ; Dutson, K. ; Dyks, J. ; Dyrda, M. ; Egberts, Kathrin ; Eger, P. ; Espigat, P. ; Fallon, L. ; Farnier, C. ; Fegan, S. ; Feinstein, F. ; Fernandes, M. V. ; Fiasson, A. ; Fontaine, G. ; Foerster, A. ; Fuessling, M. ; Gallant, Y. A. ; Gast, H. ; Gerard, L. ; Gerbig, D. ; Giebels, B. ; Glicenstein, J. F. ; Glueck, B. ; Goret, P. ; Goering, D. ; Haeffner, S. ; Hague, J. D. ; Hampf, D. ; Hauser, M. ; Heinz, S. ; Heinzelmann, G. ; Henri, G. ; Hermann, G. ; Hinton, James Anthony ; Hoffmann, A. ; Hofmann, W. ; Hofverberg, P. ; Holler, M. ; Horns, D. ; Jacholkowska, A. ; de Jager, O. C. ; Jahn, C. ; Jamrozy, M. ; Jung, I. ; Kastendieck, M. A. ; Katarzynski, K. ; Katz, U. ; Kaufmann, S. ; Keogh, D. ; Khangulyan, D. ; Khelifi, B. ; Klochkov, D. ; Kluzniak, W. ; Kneiske, T. ; Komin, Nu. ; Kosack, K. ; Kossakowski, R. ; Laffon, H. ; Lamanna, G. ; Lennarz, D. ; Lohse, T. ; Lopatin, A. ; Lu, C. -C. ; Marandon, V. ; Marcowith, Alexandre ; Masbou, J. ; Maurin, D. ; Maxted, N. ; Mayer, M. ; McComb, T. J. L. ; Medina, M. C. ; Mehault, J. ; Moderski, R. ; Moulin, Emmanuel ; Naumann, C. L. ; Naumann-Godo, M. ; de Naurois, M. ; Nedbal, D. ; Nekrassov, D. ; Nguyen, N. ; Nicholas, B. ; Niemiec, J. ; Nolan, S. J. ; Ohm, S. ; Wilhelmi, E. de Ona ; Opitz, B. ; Ostrowski, M. ; Oya, I. ; Panter, M. ; Arribas, M. Paz ; Pedaletti, G. ; Pelletier, G. ; Petrucci, P. -O. ; Pita, S. ; Puehlhofer, G. ; Punch, M. ; Quirrenbach, A. ; Raue, M. ; Rayner, S. M. ; Reimer, A. ; Reimer, O. ; Renaud, M. ; de los Reyes, R. ; Rieger, F. ; Ripken, J. ; Rob, L. ; Rosier-Lees, S. ; Rowell, G. ; Rudak, B. ; Rulten, C. B. ; Ruppel, J. ; Sahakian, V. ; Sanchez, David M. ; Santangelo, Andrea ; Schlickeiser, R. ; Schoeck, F. M. ; Schulz, A. ; Schwanke, U. ; Schwarzburg, S. ; Schwemmer, S. ; Sheidaei, F. ; Skilton, J. L. ; Sol, H. ; Spengler, G. ; Stawarz, L. ; Steenkamp, R. ; Stegmann, Christian ; Stinzing, F. ; Stycz, K. ; Sushch, Iurii ; Szostek, A. ; Tavernet, J. -P. ; Terrier, R. ; Tluczykont, M. ; Valerius, K. ; van Eldik, C. ; Vasileiadis, G. ; Venter, C. ; Vialle, J. P. ; Viana, A. ; Vincent, P. ; Voelk, H. J. ; Volpe, F. ; Vorobiov, S. ; Vorster, M. ; Wagner, S. J. ; Ward, M. ; White, R. ; Wierzcholska, A. ; Zacharias, M. ; Zajczyk, A. ; Zdziarski, A. A. ; Zech, Alraune ; Zechlin, H. -S. ; Aleksic, J. ; Antonelli, L. A. ; Antoranz, P. ; Backes, Michael ; Barrio, J. A. ; Bastieri, D. ; Becerra Gonzalez, J. ; Bednarek, W. ; Berdyugin, A. ; Berger, K. ; Bernardini, E. ; Biland, A. ; Blanch Bigas, O. ; Bock, R. K. ; Boller, A. ; Bonnoli, G. ; Tridon, D. Borla ; Braun, I. ; Bretz, T. ; Canellas, A. ; Carmona, E. ; Carosi, A. ; Colin, P. ; Colombo, E. ; Contreras, J. L. ; Cortina, J. ; Cossio, L. ; Covino, S. ; Dazzi, F. ; De Angelis, A. ; De Cea del Pozo, E. ; De Lotto, B. ; Delgado Mendez, C. ; Diago Ortega, A. ; Doert, M. ; Dominguez, A. ; Prester, Dijana Dominis ; Dorner, D. ; Doro, M. ; Elsaesser, D. ; Ferenc, D. ; Fonseca, M. V. ; Font, L. ; Fruck, C. ; Garcia Lopez, R. J. ; Garczarczyk, M. ; Garrido, D. ; Giavitto, G. ; Godinovic, N. ; Hadasch, D. ; Haefner, D. ; Herrero, A. ; Hildebrand, D. ; Hoehne-Moench, D. ; Hose, J. ; Hrupec, D. ; Huber, B. ; Jogler, T. ; Klepser, S. ; Kraehenbuehl, T. ; Krause, J. ; La Barbera, A. ; Lelas, D. ; Leonardo, E. ; Lindfors, E. ; Lombardi, S. ; Lopez, M. ; Lorenz, E. ; Makariev, M. ; Maneva, G. ; Mankuzhiyil, N. ; Mannheim, K. ; Maraschi, L. ; Mariotti, M. ; Martinez, M. ; Mazin, D. ; Meucci, M. ; Miranda, J. M. ; Mirzoyan, R. ; Miyamoto, H. ; Moldon, J. ; Moralejo, A. ; Munar, P. ; Nieto, D. ; Nilsson, K. ; Orito, R. ; Oya, I. ; Paneque, D. ; Paoletti, R. ; Pardo, S. ; Paredes, J. M. ; Partini, S. ; Pasanen, M. ; Pauss, F. ; Perez-Torres, M. A. ; Persic, M. ; Peruzzo, L. ; Pilia, M. ; Pochon, J. ; Prada, F. ; Moroni, P. G. Prada ; Prandini, E. ; Puljak, I. ; Reichardt, I. ; Reinthal, R. ; Rhode, W. ; Ribo, M. ; Rico, J. ; Ruegamer, S. ; Saggion, A. ; Saito, K. ; Saito, T. Y. ; Salvati, M. ; Satalecka, K. ; Scalzotto, V. ; Scapin, V. ; Schultz, C. ; Schweizer, T. ; Shayduk, M. ; Shore, S. N. ; Sillanpaa, A. ; Sitarek, J. ; Sobczynska, D. ; Spanier, F. ; Spiro, S. ; Stamerra, A. ; Steinke, B. ; Storz, J. ; Strah, N. ; Suric, T. ; Takalo, L. ; Takami, H. ; Tavecchio, F. ; Temnikov, P. ; Terzic, T. ; Tescaro, D. ; Teshima, M. ; Thom, M. ; Tibolla, O. ; Torres, D. F. ; Treves, A. ; Vankov, H. ; Vogler, P. ; Wagner, R. M. ; Weitzel, Q. ; Zabalza, V. ; Zandanel, F. ; Zanin, R. ; Arlen, T. ; Aune, T. ; Beilicke, M. ; Benbow, W. ; Bouvier, A. ; Bradbury, S. M. ; Buckley, J. H. ; Bugaev, V. ; Byrum, K. ; Cannon, A. ; Cesarini, A. ; Ciupik, L. ; Connolly, M. P. ; Cui, W. ; Dickherber, R. ; Duke, C. ; Errando, M. ; Falcone, A. ; Finley, J. P. ; Finnegan, G. ; Fortson, L. ; Furniss, A. ; Galante, N. ; Gall, D. ; Godambe, S. ; Griffin, S. ; Grube, J. ; Gyuk, G. ; Hanna, D. ; Holder, J. ; Huan, H. ; Hui, C. M. ; Kaaret, P. ; Karlsson, N. ; Kertzman, M. ; Khassen, Y. ; Kieda, D. ; Krawczynski, H. ; Krennrich, F. ; Lang, M. J. ; LeBohec, S. ; Maier, G. ; McArthur, S. ; McCann, A. ; Moriarty, P. ; Mukherjee, R. ; Nunez, P. D. ; Ong, R. A. ; Orr, M. ; Otte, A. N. ; Park, N. ; Perkins, J. S. ; Pichel, A. ; Pohl, Martin ; Prokoph, H. ; Ragan, K. ; Reyes, L. C. ; Reynolds, P. T. ; Roache, E. ; Rose, H. J. ; Ruppel, J. ; Schroedter, M. ; Sembroski, G. H. ; Sentuerk, G. D. ; Telezhinsky, Igor O. ; Tesic, G. ; Theiling, M. ; Thibadeau, S. ; Varlotta, A. ; Vassiliev, V. V. ; Vivier, M. ; Wakely, S. P. ; Weekes, T. C. ; Williams, D. A. ; Zitzer, B. ; de Almeida, U. Barres ; Cara, M. ; Casadio, C. ; Cheung, C. C. ; McConville, W. ; Davies, F. ; Doi, A. ; Giovannini, G. ; Giroletti, M. ; Hada, K. ; Hardee, P. ; Harris, D. E. ; Junor, W. ; Kino, M. ; Lee, N. P. ; Ly, C. ; Madrid, J. ; Massaro, F. ; Mundell, C. G. ; Nagai, H. ; Perlman, E. S. ; Steele, I. A. ; Walker, R. C. ; Wood, D. L.
The giant radio galaxy M 87 with its proximity (16 Mpc), famous jet, and very massive black hole ((3-6) x 10(9) M-circle dot) provides a unique opportunity to investigate the origin of very high energy (VHE; E > 100 GeV) gamma-ray emission generated in relativistic outflows and the surroundings of supermassive black holes. M 87 has been established as a VHE gamma-ray emitter since 2006. The VHE gamma-ray emission displays strong variability on timescales as short as a day. In this paper, results from a joint VHE monitoring campaign on M 87 by the MAGIC and VERITAS instruments in 2010 are reported. During the campaign, a flare at VHE was detected triggering further observations at VHE (H.E.S.S.), X-rays (Chandra), and radio (43 GHz Very Long Baseline Array, VLBA). The excellent sampling of the VHE gamma-ray light curve enables one to derive a precise temporal characterization of the flare: the single, isolated flare is well described by a two-sided exponential function with significantly different flux rise and decay times of tau(rise)(d) = (1.69 +/- 0.30) days and tau(decay)(d) = (0.611 +/- 0.080) days, respectively. While the overall variability pattern of the 2010 flare appears somewhat different from that of previous VHE flares in 2005 and 2008, they share very similar timescales (similar to day), peak fluxes (Phi(>0.35 TeV) similar or equal to (1-3) x 10(-11) photons cm(-2) s(-1)), and VHE spectra. VLBA radio observations of 43 GHz of the inner jet regions indicate no enhanced flux in 2010 in contrast to observations in 2008, where an increase of the radio flux of the innermost core regions coincided with a VHE flare. On the other hand, Chandra X-ray observations taken similar to 3 days after the peak of the VHE gamma-ray emission reveal an enhanced flux from the core (flux increased by factor similar to 2; variability timescale <2 days). The long-term (2001-2010) multi-wavelength (MWL) light curve of M 87, spanning from radio to VHE and including data from Hubble Space Telescope, Liverpool Telescope, Very Large Array, and European VLBI Network, is used to further investigate the origin of the VHE gamma-ray emission. No unique, common MWL signature of the three VHE flares has been identified. In the outer kiloparsec jet region, in particular in HST-1, no enhanced MWL activity was detected in 2008 and 2010, disfavoring it as the origin of the VHE flares during these years. Shortly after two of the three flares (2008 and 2010), the X-ray core was observed to be at a higher flux level than its characteristic range (determined from more than 60 monitoring observations: 2002-2009). In 2005, the strong flux dominance of HST-1 could have suppressed the detection of such a feature. Published models for VHE gamma-ray emission from M 87 are reviewed in the light of the new data.