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Prospects for Cherenkov Telescope Array Observations of the Young Supernova Remnant RX J1713.7-3946
(2017)
We perform simulations for future Cherenkov Telescope Array (CTA) observations of RX J1713.7-3946, a young supernova remnant (SNR) and one of the brightest sources ever discovered in very high energy (VHE) gamma rays. Special attention is paid to exploring possible spatial (anti) correlations of gamma rays with emission at other wavelengths, in particular X-rays and CO/H I emission. We present a series of simulated images of RX J1713.7-3946 for CTA based on a set of observationally motivated models for the gamma-ray emission. In these models, VHE gamma rays produced by high-energy electrons are assumed to trace the nonthermal X-ray emission observed by XMM-Newton, whereas those originating from relativistic protons delineate the local gas distributions. The local atomic and molecular gas distributions are deduced by the NANTEN team from CO and H I observations. Our primary goal is to show how one can distinguish the emission mechanism(s) of the gamma rays (i.e., hadronic versus leptonic, or a mixture of the two) through information provided by their spatial distribution, spectra, and time variation. This work is the first attempt to quantitatively evaluate the capabilities of CTA to achieve various proposed scientific goals by observing this important cosmic particle accelerator.
A catalog of genetic loci associated with kidney function from analyses of a million individuals
(2019)
Chronic kidney disease (CKD) is responsible for a public health burden with multi-systemic complications. Through transancestry meta-analysis of genome-wide association studies of estimated glomerular filtration rate (eGFR) and independent replication (n = 1,046,070), we identified 264 associated loci (166 new). Of these,147 were likely to be relevant for kidney function on the basis of associations with the alternative kidney function marker blood urea nitrogen (n = 416,178). Pathway and enrichment analyses, including mouse models with renal phenotypes, support the kidney as the main target organ. A genetic risk score for lower eGFR was associated with clinically diagnosed CKD in 452,264 independent individuals. Colocalization analyses of associations with eGFR among 783,978 European-ancestry individuals and gene expression across 46 human tissues, including tubulo-interstitial and glomerular kidney compartments, identified 17 genes differentially expressed in kidney. Fine-mapping highlighted missense driver variants in 11 genes and kidney-specific regulatory variants. These results provide a comprehensive priority list of molecular targets for translational research.
The Stardust mission returned cometary, interplanetary and (probably) interstellar dust in 2006 to Earth that have been analysed in Earth laboratories worldwide. Results of this mission have changed our view and knowledge on the early solar nebula. The Rosetta mission is on its way to land on comet 67P/Churyumov-Gerasimenko and will investigate for the first time in great detail the comet nucleus and its environment starting in 2014. Additional astronomy and planetary space missions will further contribute to our understanding of dust generation, evolution and destruction in interstellar and interplanetary space and provide constraints on solar system formation and processes that led to the origin of life on Earth. One of these missions, SARIM-PLUS, will provide a unique perspective by measuring interplanetary and interstellar dust with high accuracy and sensitivity in our inner solar system between 1 and 2 AU. SARIM-PLUS employs latest in-situ techniques for a full characterisation of individual micrometeoroids (flux, mass, charge, trajectory, composition()) and collects and returns these samples to Earth for a detailed analysis. The opportunity to visit again the target comet of the Rosetta mission 67P/Churyumov-Gerasimeenternko, and to investigate its dusty environment six years after Rosetta with complementary methods is unique and strongly enhances and supports the scientific exploration of this target and the entire Rosetta mission. Launch opportunities are in 2020 with a backup window starting early 2026. The comet encounter occurs in September 2021 and the reentry takes place in early 2024. An encounter speed of 6 km/s ensures comparable results to the Stardust mission.
Media coverage of the 2003 Iraq War frequently contained corrections and retractions of earlier information. For example, claims that Iraqi forces executed coalition prisoners of war after they surrendered were retracted the day after the claims were made. Similarly, tentative initial reports about the discovery of weapons of mass destruction were all later disconfirmed. We investigated the effects of these retractions and disconfirmations on people's memory for and beliefs about war-related events in two coalition countries (Australia and the United States) and one country that opposed the war (Germany). Participants were queried about (a) true events, (b) events initially presented as fact but subsequently retracted, and (c) fictional events. Participants in the United States did not show sensitivity to the correction of misinformation, whereas participants in Australia and Germany discounted corrected misinformation. Our results are consistent with previous findings in that the differences between samples reflect greater suspicion about the motives underlying the war among people in Australia and Germany than among people in the United States
Halogenated nucleobases are used as radiosensitizers in cancer radiation therapy, enhancing the reactivity of DNA to secondary low-energy electrons (LEEs). LEEs induce DNA strand breaks at specific energies (resonances) by dissociative electron attachment (DEA). Although halogenated nucleobases show intense DEA resonances at various electron energies in the gas phase, it is inherently difficult to investigate the influence of halogenated nucleobases on the actual DNA strand breakage over the broad range of electron energies at which DEA can take place (<12 eV). By using DNA origami nanostructures, we determined the energy dependence of the strand break cross-section for oligonucleotides modified with 8-bromoadenine ((8Br)A). These results were evaluated against DEA measurements with isolated (8Br)A in the gas phase. Contrary to expectations, the major contribution to strand breaks is from resonances at around 7 eV while resonances at very low energy (<2 eV) have little influence on strand breaks.
Myoviruses, bacteriophages with T4-like architecture, must contract their tails prior to DNA release. However, quantitative kinetic data on myovirus particle opening are lacking, although they are promising tools in bacteriophage-based antimicrobial strategies directed against Gram-negative hosts. For the first time, we show time-resolved DNA ejection from a bacteriophage with a contractile tail, the multi-O-antigen-specific Salmonella myovirus Det7. DNA release from Det7 was triggered by lipopolysaccharide (LPS) O-antigen receptors and notably slower than in noncontractile-tailed siphoviruses. Det7 showed two individual kinetic steps for tail contraction and particle opening. Our in vitro studies showed that highly specialized tailspike proteins (TSPs) are necessary to attach the particle to LPS. A P22-like TSP confers specificity for the Salmonella Typhimurium O-antigen. Moreover, crystal structure analysis at 1.63 angstrom resolution confirmed that Det7 recognized the Salmonella Anatum O-antigen via an E15-like TSP, DettilonTSP. DNA ejection triggered by LPS from either host showed similar velocities, so particle opening is thus a process independent of O-antigen composition and the recognizing TSP. In Det7, at permissive temperatures TSPs mediate O-antigen cleavage and couple cell surface binding with DNA ejection, but no irreversible adsorption occurred at low temperatures. This finding was in contrast to short-tailed Salmonella podoviruses, illustrating that tailed phages use common particle-opening mechanisms but have specialized into different infection niches.
Swelling and switching kinetics of gold coated end-capped poly(N-isopropylacrylamide) thin films
(2010)
Thin thermoresponsive hydrogel films of poly(N-isopropylacrylamide) end-capped with n-butyltrithiocarbonate(nbc- PNIPAM) oil si I icon supports with a gold layer on top, causing an asymmetric confinement, are investigated. For two different gold layer thicknesses (nominally 0.4 and 5 rim), the swelling and switching kinetics are probed with in situ neutron reflectivity. With a temperature jump from 23 to 40 degrees C the film is switched from a swollen into a collapsed state. For the thin gold layer this switching is faster as compared to the thick gold layer. The switching is a two-step process of water release and a subsequent structural relaxation. fit swelling and deswelling cycles, aging of the films is probed. After five cycles, the film exhibits enhanced water storage capacity. Grazing-incidence small-angle X-ray scattering (GISAXS) shows that these gold coated nbc-PNIPAM films do not age with respect to the inner structure but slightly roughen at the gold surface. As revealed by atomic force microscopy, the morphology of the gold layer is changed by the water uptake and release.
Coccolithophores have influenced the global climate for over 200 million years(1). These marine phytoplankton can account for 20 per cent of total carbon fixation in some systems(2). They form blooms that can occupy hundreds of thousands of square kilometres and are distinguished by their elegantly sculpted calcium carbonate exoskeletons (coccoliths), rendering them visible from space(3). Although coccolithophores export carbon in the form of organic matter and calcite to the sea floor, they also release CO2 in the calcification process. Hence, they have a complex influence on the carbon cycle, driving either CO2 production or uptake, sequestration and export to the deep ocean(4). Here we report the first haptophyte reference genome, from the coccolithophore Emiliania huxleyi strain CCMP1516, and sequences from 13 additional isolates. Our analyses reveal a pan genome (core genes plus genes distributed variably between strains) probably supported by an atypical complement of repetitive sequence in the genome. Comparisons across strains demonstrate that E. huxleyi, which has long been considered a single species, harbours extensive genome variability reflected in different metabolic repertoires. Genome variability within this species complex seems to underpin its capacity both to thrive in habitats ranging from the equator to the subarctic and to form large-scale episodic blooms under a wide variety of environmental conditions.
To address one of the central questions of plate tectonics-How do large transform systems work and what are their typical features?-seismic investigations across the Dead Sea Transform (DST), the boundary between the African and Arabian plates in the Middle East, were conducted for the first time. A major component of these investigations was a combined reflection/ refraction survey across the territories of Palestine, Israel and Jordan. The main results of this study are: (1) The seismic basement is offset by 3-5 km under the DST, (2) The DST cuts through the entire crust, broadening in the lower crust, (3) Strong lower crustal reflectors are imaged only on one side of the DST, (4) The seismic velocity sections show a steady increase in the depth of the crust-mantle transition (Moho) from 26 km at the Mediterranean to 39 km under the Jordan highlands, with only a small but visible, asymmetric topography of the Moho under the DST. These observations can be linked to the left-lateral movement of 105 km of the two plates in the last 17 Myr, accompanied by strong deformation within a narrow zone cutting through the entire crust. Comparing the DST and the San Andreas Fault (SAF) system, a strong asymmetry in subhorizontal lower crustal reflectors and a deep reaching deformation zone both occur around the DST and the SAF. The fact that such lower crustal reflectors and deep deformation zones are observed in such different transform systems suggests that these structures are possibly fundamental features of large transform plate boundaries
Bacteriophages use a large number of different bacterial cell envelope structures as receptors for surface attachment. As a consequence, bacterial surfaces represent a major control point for the defense against phage attack. One strategy for phage population control is the production of outer membrane vesicles (OMVs). In Gram-negative host bacteria, O-antigen-specific bacteriophages address lipopolysaccharide (LPS) to initiate infection, thus relying on an essential outer membrane glycan building block as receptor that is constantly present also in OMVs. In this work, we have analyzed interactions ofSalmonella(S.) bacteriophage P22 with OMVs. For this, we isolated OMVs that were formed in large amounts during mechanical cell lysis of the P22 S. Typhimurium host.In vitro, these OMVs could efficiently reduce the number of infective phage particles. Fluorescence spectroscopy showed that upon interaction with OMVs, bacteriophage P22 released its DNA into the vesicle lumen. However, only about one third of the phage P22 particles actively ejected their genome. For the larger part, no genome release was observed, albeit the majority of phages in the system had lost infectivity towards their host. With OMVs, P22 ejected its DNA more rapidly and could release more DNA against elevated osmotic pressures compared to DNA release triggered with protein-free LPS aggregates. This emphasizes that OMV composition is a key feature for the regulation of infective bacteriophage particles in the system.
Context. The TESS satellite was launched in 2018 to perform high-precision photometry from space over almost the whole sky in a search for exoplanets orbiting bright stars. This instrument has opened new opportunities to study variable hot subdwarfs, white dwarfs, and related compact objects. Targets of interest include white dwarf and hot subdwarf pulsators, both carrying high potential for asteroseismology. Aims. We present the discovery and detailed asteroseismic analysis of a new g-mode hot B subdwarf (sdB) pulsator, EC 21494-7018 (TIC 278659026), monitored in TESS first sector using 120-s cadence. Methods. The TESS light curve was analyzed with standard prewhitening techniques, followed by forward modeling using our latest generation of sdB models developed for asteroseismic investigations. By simultaneously best-matching all the observed frequencies with those computed from models, we identified the pulsation modes detected and, more importantly, we determined the global parameters and structural configuration of the star. Results. The light curve analysis reveals that EC 21494-7018 is a sdB pulsator counting up to 20 frequencies associated with independent g-modes. The seismic analysis singles out an optimal model solution in full agreement with independent measurements provided by spectroscopy (atmospheric parameters derived from model atmospheres) and astrometry (distance evaluated from Gaia DR2 trigonometric parallax). Several key parameters of the star are derived. Its mass (0.391 +/- 0.009x2006;M-circle dot) is significantly lower than the typical mass of sdB stars and suggests that its progenitor has not undergone the He-core flash; therefore this progenitor could originate from a massive (greater than or similar to 2;M-circle dot) red giant, which is an alternative channel for the formation of sdBs. Other derived parameters include the H-rich envelope mass (0.0037 +/- 0.0010;M-circle dot), radius (0.1694 +/- 0.0081;R-circle dot), and luminosity (8.2 +/- 1.1;L-circle dot). The optimal model fit has a double-layered He+H composition profile, which we interpret as an incomplete but ongoing process of gravitational settling of helium at the bottom of a thick H-rich envelope. Moreover, the derived properties of the core indicate that EC 21494-7018 has burnt similar to 43% (in mass) of its central helium and possesses a relatively large mixed core (M-core;=;0.198 +/- 0.010;M-circle dot), in line with trends already uncovered from other g-mode sdB pulsators analyzed with asteroseismology. Finally, we obtain for the first time an estimate of the amount of oxygen (in mass; X(O)(core) = 0.16(-0.05)(+0.13)X(O)core=0.16-0.05+0.13$ X(mathrm{O})_{mathrm{core}}=0.16_{-0.05}<^>{+0.13} $) produced at this stage of evolution by an helium-burning core. This result, along with the core-size estimate, is an interesting constraint that may help to narrow down the still uncertain C-12(alpha,;gamma)O-16 nuclear reaction rate.
EVR-CB-001: An Evolving, Progenitor, White Dwarf Compact Binary Discovered with the Evryscope
(2019)
We present EVR-CB-001, the discovery of a compact binary with an extremely low-mass (0.21 +/- 0.05M(circle dot)) helium core white dwarf progenitor (pre-He WD) and an unseen low-mass (0.32 +/- 0.06M(circle dot)) helium white dwarf (He WD) companion. He WDs are thought to evolve from the remnant helium-rich core of a main-sequence star stripped during the giant phase by a close companion. Low-mass He WDs are exotic objects (only about 0.2% of WDs are thought to be less than 0.3 M-circle dot), and are expected to be found in compact binaries. Pre-He WDs are even rarer, and occupy the intermediate phase after the core is stripped, but before the star becomes a fully degenerate WD and with a larger radius (approximate to 0.2R(circle dot)) than a typical WD. The primary component of EVR-CB-001 (the pre-He WD) was originally thought to be a hot subdwarf (sdB) star from its blue color and under-luminous magnitude, characteristic of sdBs. The mass, temperature (T-eff = 18,500 +/- 500 K), and surface gravity (log(g) = 4.96 +/- 0.04) solutions from this work are lower than values for typical hot subdwarfs. The primary is likely to be a post-red-giant branch, pre-He WD contracting into a He WD, and at a stage that places it nearest to sdBs on color-magnitude and T-eff-log(g) diagrams. EVR-CB-001 is expected to evolve into a fully double degenerate, compact system that should spin down and potentially evolve into a single hot subdwarf star. Single hot subdwarfs are observed, but progenitor systems have been elusive.
The presence of extreme horizontal branch (EHB) and blue hook stars in some Galactic globular clusters (GGCs) constitutes one of the remaining mysteries of stellar evolution. While several evolutionary scenarios have been proposed to explain the characteristics of this peculiar population of evolved stars, their observational verification has been limited by the availability of spectroscopic data for a statistically significant sample of such objects in any single GGC. We recently launched the SHOTGLAS project with the aim of providing a comprehensive picture of this intriguing stellar population in terms of spectroscopic properties for all readily accessible GGCs hosting an EHB. In this first paper, we focus on omega Cen, a peculiar, massive GGC that hosts multiple stellar populations. We use non-LTE model atmospheres to derive atmospheric parameters (Te ff, log g and N(He) / N(H)) and spectroscopic masses for 152 EHB stars in the cluster. This constitutes the largest spectroscopic sample of EHB stars ever analyzed in a GGC and represents similar to 20% of the EHB population of omega Cen. We also search for close binaries among these stars based on radial velocity variations. Our results show that the EHB population of omega Cen is divided into three spectroscopic groups that are very distinct in the Te ff helium abundance plane. The coolest sdB-type stars (Te ff. 30 000 K) have a hydrogen-rich atmosphere, populate the theoretical EHB region in the Te ff log g plane, and form 26% of our sample. The hottest sdO-type stars (Te ff & 42 000 K) make up 10% of the sample, have a hydrogen-rich atmosphere and are thought to be in a post-EHB evolutionary phase. The majority of our sample is found at intermediate temperatures and consists of sdOB stars that have roughly solar or super-solar atmospheric helium abundances. It is these objects that constitute the blue hook at V > 18 : 5 mag in the omega Cen color-magnitude diagram. Interestingly, the helium-enriched sdOBs do not have a significant counterpart population in the Galactic field, indicating that their formation is dependent on the particular environment found in omega Cen and other select GGCs. Another major di ff erence between the EHB stars in omega Cen and the field is the fraction of close binaries. From our radial velocity survey we identify two binary candidates, however no orbital solutions could be determined. We estimate an EHB close binary fraction of similar to 5% in omega Cen. This low fraction is in line with findings for other GGCs, but in sharp contrast to the situation in the field, where around 50% of the sdB stars reside in close binaries. Finally, the mass distribution derived is very similar for all three spectroscopic groups, however the average mass (0.38 M fi) is lower than that expected from stellar evolution theory. While this mass conundrum has previously been noted for EHB stars in omega Cen, it so far appears to be unique to that cluster.
The presence of ultrahigh excitation (UHE) absorption lines (e.g. OVIII) in the optical spectra of several of the hottest white dwarfs poses a decades-long mystery and is something that has never been observed in any other astrophysical object. The occurrence of such features requires a dense environment with temperatures near 10(6) K, by far exceeding the stellar effective temperature. Here we report the discovery of a new hot wind white dwarf, GALEXJ014636.8+323615. Astonishingly, we found for the first time rapid changes of the equivalent widths of the UHE features, which are correlated to the rotational period of the star (P=0.242035 d). We explain this with the presence of a wind-fed circumstellar magnetosphere in which magnetically confined wind shocks heat up the material to the high temperatures required for the creation of the UHE lines. The photometric and spectroscopic variability of GALEXJ014636.8+323615 can then be understood as consequence of the obliquity of the magnetic axis with respect to the rotation axis of the white dwarf. This is the first time a wind-fed circumstellar magnetosphere around an apparently isolated white dwarf has been discovered and finally offers a plausible explanation of the ultrahot wind phenomenon.
No association of goal-directed and habitual control with alcohol consumption in young adults
(2017)
Alcohol dependence is a mental disorder that has been associated with an imbalance in behavioral control favoring model-free habitual over model-based goal-directed strategies. It is as yet unknown, however, whether such an imbalance reflects a predisposing vulnerability or results as a consequence of repeated and/or excessive alcohol exposure. We, therefore, examined the association of alcohol consumption with model-based goal-directed and model-free habitual control in 188 18-year-old social drinkers in a two-step sequential decision-making task while undergoing functional magnetic resonance imaging before prolonged alcohol misuse could have led to severe neurobiological adaptations. Behaviorally, participants showed a mixture of model-free and model-based decision-making as observed previously. Measures of impulsivity were positively related to alcohol consumption. In contrast, neither model-free nor model-based decision weights nor the trade-off between them were associated with alcohol consumption. There were also no significant associations between alcohol consumption and neural correlates of model-free or model-based decision quantities in either ventral striatum or ventromedial prefrontal cortex. Exploratory whole-brain functional magnetic resonance imaging analyses with a lenient threshold revealed early onset of drinking to be associated with an enhanced representation of model-free reward prediction errors in the posterior putamen. These results suggest that an imbalance between model-based goal-directed and model-free habitual control might rather not be a trait marker of alcohol intake per se.
Rapid decline of glomerular filtration rate estimated from creatinine (eGFRcrea) is associated with severe clinical endpoints. In contrast to cross-sectionally assessed eGFRcrea, the genetic basis for rapid eGFRcrea decline is largely unknown. To help define this, we meta-analyzed 42 genome-wide association studies from the Chronic Kidney Diseases Genetics Consortium and United Kingdom Biobank to identify genetic loci for rapid eGFRcrea decline. Two definitions of eGFRcrea decline were used: 3 mL/min/1.73m(2)/year or more ("Rapid3"; encompassing 34,874 cases, 107,090 controls) and eGFRcrea decline 25% or more and eGFRcrea under 60 mL/min/1.73m(2) at follow-up among those with eGFRcrea 60 mL/min/1.73m(2) or more at baseline ("CKDi25"; encompassing 19,901 cases, 175,244 controls). Seven independent variants were identified across six loci for Rapid3 and/or CKDi25: consisting of five variants at four loci with genome-wide significance (near UMOD-PDILT (2), PRKAG2, WDR72, OR2S2) and two variants among 265 known eGFRcrea variants (near GATM, LARP4B). All these loci were novel for Rapid3 and/or CKDi25 and our bioinformatic follow-up prioritized variants and genes underneath these loci. The OR2S2 locus is novel for any eGFRcrea trait including interesting candidates. For the five genome-wide significant lead variants, we found supporting effects for annual change in blood urea nitrogen or cystatin-based eGFR, but not for GATM or (LARP4B). Individuals at high compared to those at low genetic risk (8-14 vs. 0-5 adverse alleles) had a 1.20-fold increased risk of acute kidney injury (95% confidence interval 1.08-1.33). Thus, our identified loci for rapid kidney function decline may help prioritize therapeutic targets and identify mechanisms and individuals at risk for sustained deterioration of kidney function.
Rapid decline of glomerular filtration rate estimated from creatinine (eGFRcrea) is associated with severe clinical endpoints. In contrast to cross-sectionally assessed eGFRcrea, the genetic basis for rapid eGFRcrea decline is largely unknown. To help define this, we meta-analyzed 42 genome-wide association studies from the Chronic Kidney Diseases Genetics Consortium and United Kingdom Biobank to identify genetic loci for rapid eGFRcrea decline. Two definitions of eGFRcrea decline were used: 3 mL/min/1.73m(2)/year or more ("Rapid3"; encompassing 34,874 cases, 107,090 controls) and eGFRcrea decline 25% or more and eGFRcrea under 60 mL/min/1.73m(2) at follow-up among those with eGFRcrea 60 mL/min/1.73m(2) or more at baseline ("CKDi25"; encompassing 19,901 cases, 175,244 controls). Seven independent variants were identified across six loci for Rapid3 and/or CKDi25: consisting of five variants at four loci with genome-wide significance (near UMOD-PDILT (2), PRKAG2, WDR72, OR2S2) and two variants among 265 known eGFRcrea variants (near GATM, LARP4B). All these loci were novel for Rapid3 and/or CKDi25 and our bioinformatic follow-up prioritized variants and genes underneath these loci. The OR2S2 locus is novel for any eGFRcrea trait including interesting candidates. For the five genome-wide significant lead variants, we found supporting effects for annual change in blood urea nitrogen or cystatin-based eGFR, but not for GATM or (LARP4B). Individuals at high compared to those at low genetic risk (8-14 vs. 0-5 adverse alleles) had a 1.20-fold increased risk of acute kidney injury (95% confidence interval 1.08-1.33). Thus, our identified loci for rapid kidney function decline may help prioritize therapeutic targets and identify mechanisms and individuals at risk for sustained deterioration of kidney function.
In detoxified alcohol-dependent patients, alcohol-related stimuli can promote relapse. However, to date, the mechanisms by which contextual stimuli promote relapse have not been elucidated in detail. One hypothesis is that such contextual stimuli directly stimulate the motivation to drink via associated brain regions like the ventral striatum and thus promote alcohol seeking, intake and relapse. Pavlovian-to-Instrumental-Transfer (PIT) may be one of those behavioral phenomena contributing to relapse, capturing how Pavlovian conditioned (contextual) cues determine instrumental behavior (e.g. alcohol seeking and intake). We used a PIT paradigm during functional magnetic resonance imaging to examine the effects of classically conditioned Pavlovian stimuli on instrumental choices in n=31 detoxified patients diagnosed with alcohol dependence and n=24 healthy controls matched for age and gender. Patients were followed up over a period of 3 months. We observed that (1) there was a significant behavioral PIT effect for all participants, which was significantly more pronounced in alcohol-dependent patients; (2) PIT was significantly associated with blood oxygen level-dependent (BOLD) signals in the nucleus accumbens (NAcc) in subsequent relapsers only; and (3) PIT-related NAcc activation was associated with, and predictive of, critical outcomes (amount of alcohol intake and relapse during a 3 months follow-up period) in alcohol-dependent patients. These observations show for the first time that PIT-related BOLD signals, as a measure of the influence of Pavlovian cues on instrumental behavior, predict alcohol intake and relapse in alcohol dependence.