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Rezensiertes Werk: Basten, Ludger: Postmoderner Urbanismus : Gestaltung in der städtischen Peripherie / Ludger Basten. - Münster : LIT, 2005. - X, 363 S. : Ill. - ( Schriften des Arbeitskreises Stadtzukünfte der Deutschen Gesellschaft für Geographie ; 1) Zugl.: Bochum, Univ., Habil.-Schr., 2005 ISBN 3-8258-8902-5
Rezensierte Werke: Stegmann, Bernd-Achim: Großstadt im Image : eine wahrnehmungsgeographische Studie zu raumbezogenen Images und zum Imagemarketing in Printmedien am Beispiel Kölns und seiner Stadtviertel / Bernd-Achim Stegmann. - 1. Aufl. - Köln : Geograph. Inst. der Univ., 1997. - XII, 219 S. : Ill., graph. Darst., Kt. - (Kölner geographische Arbeiten ; 68) Zugl.: Köln, Univ., Diss., 1995 Körner, Wilfried: Der Frankfurter Grüngürtel als sozialer Raum : Diskurse, Raumbilder und Netzwerke - das Beispiel Sossenheim / von Wilfried Körner. - Frankfurt a.M. : Inst. für Kulturgeographie, Stadt- und Regionalforschung der J.-W.-Goethe-Univ., 1998. - 152 S. : Ill., Ktn. - (Materialien / Institut für Kulturgeographie, Stadt- und Regionalforschung der J.W. Goethe-Universität Frankfurt am Main ; 23) Nebent.: Frankfurter Grüngürtel ISBN 3-923218-16-8 Schüttemeyer, Anke: Eigen- und Fremdimage der Stadt Bonn : eine empirische Untersuchung / Anke Schüttemeyer. Geographisches Institut, Rheinische Friedrich-Wilhelms-Universität Bonn. - Bonn : Geograph. Inst., Lehrstuhl Grotz, 1998. - 109 S. : graph. Darst., Kt. - (Bonner Beiträge zur Geographie ; H. 9) Ganser, Karl: Liebe auf den zweiten Blick : Internationale Bauausstellung Emscher Park / Karl Ganser. - Dortmund : Harenberg, 1999. - 223 S. : Ill.; Kt. ISBN 3-611-00824-9 Kunst setzt Zeichen : Landmarken-Kunst ; Ausstellung vom 1. Mai bis Oktober 1999 in der Ludwig Galerie Schloss Oberhausen / hrsg. von Peter Pachnicke und Bernhard Mensch. - Oberhausen : Ludwig Galerie Schloß Oberhausen, 1999. - 213 S. : zahlr. Ill. ISBN 3-932236-07-6 Ausstellung vom 14. April bis 1. August 1999 im Rheinischen Industriemuseum Oberhausen / Daniel Stemmrich, Uwe Sülflohn (Red.). - Heidelberg, 1999. - 202 S. - (Schriften des Rheinischen Industriemuseums ; Bd. 16)
The poly(A) tail at 3’ ends of eukaryotic mRNAs promotes their nuclear export, stability and translational efficiency, and changes in its length can strongly impact gene expression. The Arabidopsis thaliana genome encodes three canonical nuclear poly(A) polymerases, PAPS1, PAPS2 and PAPS4. As shown by their different mutant phenotypes, these three isoforms are functionally specialized, with PAPS1 modifying organ growth and suppressing a constitutive immune response. However, the molecular basis of this specialization is largely unknown. Here, we have estimated poly(A)-tail lengths on a transcriptome-wide scale in wild-type and paps1 mutants. This identified categories of genes as particularly strongly affected in paps1 mutants, including genes encoding ribosomal proteins, cell-division factors and major carbohydrate-metabolic proteins. We experimentally verified two novel functions of PAPS1 in ribosome biogenesis and redox homoeostasis that were predicted based on the analysis of poly(A)-tail length changes in paps1 mutants. When overlaying the PAPS1-dependent effects observed here with coexpression analysis based on independent microarray data, the two clusters of transcripts that are most closely coexpressed with PAPS1 show the strongest change in poly(A)-tail length and transcript abundance in paps1 mutants in our analysis. This suggests that their coexpression reflects at least partly the preferential polyadenylation of these transcripts by PAPS1 versus the other two poly(A)-polymerase isoforms. Thus, transcriptome-wide analysis of poly(A)-tail lengths identifies novel biological functions and likely target transcripts for polyadenylation by PAPS1. Data integration with large-scale co-expression data suggests that changes in the relative activities of the isoforms are used as an endogenous mechanism to co-ordinately modulate plant gene expression.
The poly(A) tail at 3’ ends of eukaryotic mRNAs promotes their nuclear export, stability and translational efficiency, and changes in its length can strongly impact gene expression. The Arabidopsis thaliana genome encodes three canonical nuclear poly(A) polymerases, PAPS1, PAPS2 and PAPS4. As shown by their different mutant phenotypes, these three isoforms are functionally specialized, with PAPS1 modifying organ growth and suppressing a constitutive immune response. However, the molecular basis of this specialization is largely unknown. Here, we have estimated poly(A)-tail lengths on a transcriptome-wide scale in wild-type and paps1 mutants. This identified categories of genes as particularly strongly affected in paps1 mutants, including genes encoding ribosomal proteins, cell-division factors and major carbohydrate-metabolic proteins. We experimentally verified two novel functions of PAPS1 in ribosome biogenesis and redox homoeostasis that were predicted based on the analysis of poly(A)-tail length changes in paps1 mutants. When overlaying the PAPS1-dependent effects observed here with coexpression analysis based on independent microarray data, the two clusters of transcripts that are most closely coexpressed with PAPS1 show the strongest change in poly(A)-tail length and transcript abundance in paps1 mutants in our analysis. This suggests that their coexpression reflects at least partly the preferential polyadenylation of these transcripts by PAPS1 versus the other two poly(A)-polymerase isoforms. Thus, transcriptome-wide analysis of poly(A)-tail lengths identifies novel biological functions and likely target transcripts for polyadenylation by PAPS1. Data integration with large-scale co-expression data suggests that changes in the relative activities of the isoforms are used as an endogenous mechanism to co-ordinately modulate plant gene expression.
The poly(A) tail at 3' ends of eukaryotic mRNAs promotes their nuclear export, stability and translational efficiency, and changes in its length can strongly impact gene expression. The Arabidopsis thaliana genome encodes three canonical nuclear poly(A) polymerases, PAPS1, PAPS2 and PAPS4. As shown by their different mutant phenotypes, these three isoforms are functionally specialized, with PAPS1 modifying organ growth and suppressing a constitutive immune response. However, the molecular basis of this specialization is largely unknown. Here, we have estimated poly(A)-tail lengths on a transcriptome-wide scale in wild-type and paps1 mutants. This identified categories of genes as particularly strongly affected in paps1 mutants, including genes encoding ribosomal proteins, cell-division factors and major carbohydrate-metabolic proteins. We experimentally verified two novel functions of PAPS1 in ribosome biogenesis and redox homoeostasis that were predicted based on the analysis of poly(A)-tail length changes in paps1 mutants. When overlaying the PAPS1-dependent effects observed here with coexpression analysis based on independent microarray data, the two clusters of transcripts that are most closely coexpressed with PAPS1 show the strongest change in poly(A)-tail length and transcript abundance in paps1 mutants in our analysis. This suggests that their coexpression reflects at least partly the preferential polyadenylation of these transcripts by PAPS1 versus the other two poly(A)-polymerase isoforms. Thus, transcriptome-wide analysis of poly(A)-tail lengths identifies novel biological functions and likely target transcripts for polyadenylation by PAPS1. Data integration with large-scale co-expression data suggests that changes in the relative activities of the isoforms are used as an endogenous mechanism to co-ordinately modulate plant gene expression.