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Electrochemical methods offer the simple characterization of the synthesis of molecularly imprinted polymers (MIPs) and the readouts of target binding. The binding of electroinactive analytes can be detected indirectly by their modulating effect on the diffusional permeability of a redox marker through thin MIP films. However, this process generates an overall signal, which may include nonspecific interactions with the nonimprinted surface and adsorption at the electrode surface in addition to (specific) binding to the cavities. Redox-active low-molecular-weight targets and metalloproteins enable a more specific direct quantification of their binding to MIPs by measuring the faradaic current. The in situ characterization of enzymes, MIP-based mimics of redox enzymes or enzyme-labeled targets, is based on the indication of an electroactive product. This approach allows the determination of both the activity of the bio(mimetic) catalyst and of the substrate concentration.
Plans are currently being drafted for the next decade of action on biodiversity-both the post-2020 Global Biodiversity Framework of the Convention on Biological Diversity (CBD) and Biodiversity Strategy of the European Union (EU). Freshwater biodiversity is disproportionately threatened and underprioritized relative to the marine and terrestrial biota, despite supporting a richness of species and ecosystems with their own intrinsic value and providing multiple essential ecosystem services. Future policies and strategies must have a greater focus on the unique ecology of freshwater life and its multiple threats, and now is a critical time to reflect on how this may be achieved. We identify priority topics including environmental flows, water quality, invasive species, integrated water resources management, strategic conservation planning, and emerging technologies for freshwater ecosystem monitoring. We synthesize these topics with decades of first-hand experience and recent literature into 14 special recommendations for global freshwater biodiversity conservation based on the successes and setbacks of European policy, management, and research. Applying and following these recommendations will inform and enhance the ability of global and European post-2020 biodiversity agreements to halt and reverse the rapid global decline of freshwater biodiversity.
The identification of buried soil horizons in agricultural landscapes helps to quantify sediment budgets and erosion-related carbon dynamics. High-resolution mapping of buried horizons using conventional soil surveys is destructive and time consuming. Geoelectrical sensors can offer a fast and non-destructive alternative for determining horizon positions and properties. In this paper, we compare the suitability of several geoelectrical methods for measuring the depth to buried horizons (Apb, Ahb and Hab) in the hummocky ground moraine landscape of northeastern Germany. Soil profile descriptions were developed for 269 locations within a 6-ha experimental field "CarboZALF-D". A stepwise linear discriminant analysis (LDA) estimated the lateral position of the buried horizons using electromagnetic induction data and terrain attributes. To predict the depth of a buried horizon, multiple linear regression (MLR) was used for both a 120-m transect and a 0.2-ha pseudo-three-dimensional (3D) area. At these scales, apparent electrical conductivity (ECa), electrical resistivity (ER) and terrain attributes were used as independent variables. The LDA accurately predicted Apb- and Ahb-horizons (a correct classification of 93%). The LDA of the Hab-horizon had a misclassification of 24%, which was probably related to the smaller test set and the higher depth of this horizon. The MLR predicted the depth of the Apb-, Ahb- and Hab-horizons with relative root mean square errors (RMSEs) of 7, 3 and 13%, respectively, in the pseudo-3D area. MLR had a lower accuracy for the 2D transect compared to the pseudo-3D area. Overall, the use of LDA and MLR has been an efficient methodological approach for predicting buried horizon positions. Highlights The suitability of geoelectrical measurements for digital modelling of diagnostic buried soil horizons was determined. LDA and MLR were used to detect multiple horizons with geoelectrical devices and terrain attributes. Geoelectrical variables were significant predictors of the position of the target soil horizons. The use of these tested digital technologies gives an opportunity to develop high-resolution soil mapping procedures.