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- Social origin (2)
- Soft Law (2)
- Solarzellen (2)
- Solidago canadensis (2)
- Solidago gigantea (2)
- Somatic (2)
- South Asia (2)
- South Korea (2)
- Soziale Herkunft (2)
- Sozialer Survey Österreich (2)
- Sozialkapital (2)
- Spaltsätze (2)
- Speed (2)
- Sprachtherapie (2)
- Sputum (2)
- Staatsgrenzen (2)
- Standardized patients (2)
- Staphylococcus aureus (2)
- Strain measurement (2)
- Stratigraphy (2)
- Studienabbruch (2)
- Studienerfolg (2)
- Subduction (2)
- Survey Research Methods (2)
- Sustainability (2)
- Switzerland (2)
- Südkorea (2)
- TAVI (2)
- TEM (2)
- Tableau Physique (2)
- Tacitus (2)
- Terminologie und Technologie der antiken Weberei (2)
- TerraceM (2)
- Textbooks (2)
- Thermal effects (2)
- Toll and Imd pathways (2)
- Transcriptome assembly (2)
- Transformation (2)
- Translation (2)
- Treatment pathways (2)
- UV radiation (2)
- UX (2)
- Ultrafast X-rays (2)
- Uncertainties (2)
- Union (2)
- Universidad Incca de Colombia (2)
- Unsicherheiten (2)
- Urbanization (2)
- Utopie (2)
- VIIRS DNB (2)
- Venom proteins (2)
- Verkhoyansk mountains (2)
- Verwaltung (2)
- Visual system (2)
- Vues des Cordillères (2)
- Vulnerabilität (2)
- Völkerrecht (2)
- Weather (2)
- Weberei (2)
- Weight (2)
- Weight bias internalization (2)
- West Africa (2)
- West Germany (2)
- Western Interior Basin (2)
- Western Qaidam Basin (2)
- Widerstandstraining (2)
- Wind (2)
- Wissensmanagement (2)
- X-ray absorption (2)
- X-ray emission (2)
- X-ray photoelectron spectroscopy (2)
- X-ray probe (2)
- XRF (2)
- Zweitveröffentlichung (2)
- absorption (2)
- academic leadership (2)
- academic-achievement (2)
- accountability (2)
- acid sphingomyelinase (2)
- acoustic variability (2)
- actin polymerization (2)
- action processing (2)
- activation (2)
- activator–inhibitor models (2)
- actuation (2)
- acute alcohol consumption (2)
- acute chronic workload ratio (2)
- acute coronary syndrome (2)
- acute kidney injury (2)
- adaptive force (2)
- addiction (2)
- adiponectin (2)
- adiposity (2)
- adjectives (2)
- adolescence (2)
- adsorption (2)
- aerobic power (2)
- affect (2)
- age (2)
- ageing (2)
- agent (2)
- aggregation (2)
- aggression (2)
- agro-infiltration (2)
- airglow (2)
- alexithymia (2)
- alkylphospholipids (2)
- all-cause mortality (2)
- allometry (2)
- alphaherpesvirus (2)
- alternaria group (2)
- alternative rewards (2)
- alumni work (2)
- analytics (2)
- anchor peptides (2)
- ancient sedimentary DNA (2)
- animal and computational models (2)
- animal behavior (2)
- answer set programming (2)
- anthropogenic food subsidies (2)
- anthropometric measures (2)
- antimicrobial peptide (2)
- antonymy (2)
- anxiety (2)
- anxiety-like behavior (2)
- apis mellifera (2)
- apodemus-agrarius (2)
- apple (2)
- apple replant disease (2)
- approximation (2)
- aquatic ecosystems (2)
- aquatic fungi (2)
- aqueous dispersion (2)
- aqueous-solution (2)
- aquifer (2)
- architecture (2)
- area-based conservation (2)
- arthropod (2)
- articulation (2)
- artificial light (2)
- artificial muscles (2)
- assault (2)
- assembly processes (2)
- assembly rules (2)
- associations (2)
- astronomical calibration (2)
- astroparticle physics (2)
- atmospheric blocking (2)
- atomic force microscopy (AFM) (2)
- attitudes (2)
- attitudes toward intimate partner violence (2)
- autocorrelation (2)
- automatic processing (2)
- auxotrophy (2)
- avian personalities (2)
- axillary bud (2)
- azobenzene (2)
- balance (2)
- barbell velocity (2)
- base excision repair (incision activity) (2)
- batch process (2)
- bias (2)
- bibliometrics (2)
- bidirectional reflectance (2)
- bifurcation theory (2)
- biochemistry (2)
- biodegradable polymers (2)
- biodiversity facets (2)
- biofortification (2)
- biological invasions (2)
- biological maturation (2)
- biological soil crusts (2)
- biomarker (2)
- biomechanics (2)
- biorefineries (2)
- biostimulant (2)
- biotic filtering (2)
- biotin sulfoxide reductase (2)
- body image (2)
- body waves (2)
- body weight gain (2)
- bone mineral density (2)
- bone pathologies (2)
- borehole leakage (2)
- brain (2)
- branching (2)
- broadband and narrowband dimensions of behavior (2)
- bullying victimization (2)
- business (2)
- cadmium-free (2)
- calcium influx (2)
- calibration (2)
- cancer epidemiology (2)
- capacities (2)
- carbohydrate‐ based (2)
- carbon (2)
- carbon cycling (2)
- carbon dots (2)
- carbon-cycle (2)
- carbonaceous aerosols (2)
- carbonate (2)
- carotenoid biosynthesis (2)
- carotenoids (2)
- carrion ecology (2)
- carryover effects (2)
- cartography (2)
- catalysts (2)
- catchment (2)
- cell cycle (2)
- cell-based in vitro assay (2)
- cells (2)
- cellular bioimaging (2)
- cement (2)
- central fixation bias (2)
- central south island (2)
- ceramide (2)
- cesium lead halides (2)
- chains (2)
- channel (2)
- characteristics (2)
- charcoal (2)
- child abuse (2)
- chitosan (2)
- chlorophyll a (2)
- chloroplast (2)
- cholesterol (2)
- chromatin (2)
- chromatin regulation (2)
- chronology (AICC2012) (2)
- classical conditioning (2)
- classical reception (2)
- click chemistry (2)
- client preferences (2)
- climate change impacts (2)
- climate dynamics (2)
- climate networks (2)
- clonal complex (2)
- co-limitation (2)
- coagulation (2)
- coarticulation (2)
- coefficient (2)
- coffee by-products (2)
- cognition (2)
- cognitive-behavioral control (2)
- cognitive-control (2)
- collaborative consumption (2)
- college students (2)
- colonialism (2)
- colony viability (2)
- coloring agents (2)
- combat sport (2)
- common ground (2)
- common ownership (2)
- common‐garden experiment (2)
- communities (2)
- comparison (2)
- comparison of devices (2)
- complex networks (2)
- composites (2)
- composition (2)
- comprehension (2)
- computer-based training (2)
- concepts (2)
- concurrent training (2)
- conduct problems (2)
- confidence (2)
- conformational rearrangement (2)
- consequences (2)
- constraint-based modeling (2)
- consumptive resistance (2)
- continental lithosphere (2)
- continuity (2)
- continuous process (2)
- continuous time random walk (2)
- contract workers (2)
- contrast effect (2)
- conversational implicature (2)
- converting factor (2)
- cooperation (2)
- coordination (2)
- copper-related disorders (2)
- core PG1351 (2)
- cori cycle (2)
- coronary artery disease (2)
- coronary heart disease (2)
- corporate citizenship (2)
- corporate foresight (2)
- corporate social responsibility (2)
- cortisol (2)
- coupled surface (2)
- craving and relapse (2)
- critical solution temperature (2)
- crop diversity (2)
- cropping system (2)
- cross ownership (2)
- crust (2)
- cryptic species complex (2)
- crystal orientation (2)
- culture (2)
- cyanobacteria (2)
- cyanobacterial sucrose-phosphatase (2)
- cyber victimization (2)
- cyberbullying victimization (2)
- cysteine alkylation (2)
- dark triad (2)
- dark virus (2)
- data (2)
- data cleaning (2)
- data fusion (2)
- data matching (2)
- data preparation (2)
- data processing (2)
- data sharing (2)
- data wrangling (2)
- database (2)
- de novo genome assembly (2)
- decarbonization (2)
- decision-theory (2)
- decline (2)
- deep biosphere (2)
- deep-sea (2)
- deformation monitoring (2)
- dehydration (2)
- density (2)
- density functional theory (2)
- dependence (2)
- depressive-like behavior (2)
- derivation (2)
- derivational complexity (2)
- development (2)
- developmental dyscalculia (2)
- developmental dyslexia (2)
- developmental trajectories (2)
- dietary patterns (2)
- dietary restriction (2)
- diffraction (2)
- digital bathymetric model (2)
- digitales Lernen (2)
- digitalization (2)
- dimension independent bound (2)
- dimerization of 4-nitrothiophenol (2)
- disaster risk reduction (2)
- discrepancy principle (2)
- disease (2)
- diselenide (2)
- disorders (2)
- dispersal filtering (2)
- diversity climate (2)
- donor (2)
- dopamine (2)
- drained lake basins (2)
- drought index (2)
- drug delivery system (2)
- dual GLP-1/glucagon receptor agonist (2)
- duplicate detection (2)
- dust (2)
- dust sources (2)
- dyadic coping (2)
- dynamic capabilities (2)
- dynamical model (2)
- dynamical models (2)
- dyslexia (2)
- e-z reader (2)
- early eocene (2)
- early warning (2)
- earth system model (2)
- earthquake (2)
- earthquake source observations (2)
- eastern continental Asia (2)
- eco-hydrology (2)
- ecological novelty (2)
- ecological speciation (2)
- econometrics (2)
- ecosystem services provisioning (2)
- edge computing (2)
- effectiveness (2)
- effects (2)
- efficiency (2)
- efficient (2)
- electric fish (2)
- electron-transfer (2)
- electropolymerization (2)
- emission factors (2)
- emotion regulation (2)
- emotional difficulties (2)
- emotions (2)
- empirical mode decomposition (2)
- emulsion (2)
- end-stage kidney disease (2)
- endotoxin (2)
- energy (2)
- energy storage (2)
- enrichment experiments (2)
- ensemble and time averaged mean squared displacement (2)
- entity resolution (2)
- entropy production (2)
- enzyme (2)
- eocene thermal maximum (2)
- epigenetic variation (2)
- episodic memory (2)
- equatorial electrojet (2)
- equifinality (2)
- equine (2)
- erosion hotspots (2)
- error reduction (2)
- ethnic social capital (2)
- ethnic-racial identity (2)
- europe (2)
- european (2)
- evaluative study (2)
- evaporation (2)
- evaporites (2)
- event expectations (2)
- evidence (2)
- evolutionary (2)
- evolutionary ecology (2)
- excitonic materials (2)
- executive function (2)
- executive functions (2)
- exendin-4 (2)
- exercise intensity (2)
- exercise therapy (2)
- exhaustivity (2)
- existence (2)
- expanding medium (2)
- expectancies (2)
- experiences survey (2)
- experimental evidence (2)
- exploit (2)
- exploratory-behavior (2)
- exposed water ice (2)
- exposome (2)
- exposome‐ wide association study (2)
- expression patterns (2)
- external training load (2)
- externalization (2)
- extinction drivers (2)
- extinction event (2)
- extremal values (2)
- eye tracking (2)
- family farming (2)
- fasciation (2)
- fastest first-passage time of N walkers (2)
- fault creep (2)
- feedback (2)
- feeding behaviour (2)
- ferroelectric polymers (2)
- fetal origins hypothesis (2)
- fiber actuators (2)
- field-aligned currents (2)
- filter (2)
- fine dining (2)
- firmware update (2)
- fitness (2)
- fitness consequences (2)
- fixation locations (2)
- fièvre jaune (2)
- flagellum (2)
- flat-slab subduction (2)
- floating mat (2)
- flood (2)
- flood recovery (2)
- flood risk (2)
- flooded grasslands (2)
- floodplains (2)
- fluctuations (2)
- fluid injection (2)
- fluorescence (2)
- fluorescence correlation spectroscopy (2)
- fluorinated organic spacer (2)
- flux tracking (2)
- focus group (2)
- food frequency questionnaire (2)
- forage availability (2)
- forage gaps (2)
- foraging (2)
- force (2)
- force profiles (2)
- forebrain (2)
- foreign language (2)
- formal verification (2)
- formate dehydrogenase (2)
- freshwater (2)
- freshwater heterotrophic bacteria (2)
- frictional properties (2)
- fsQCA (2)
- functional richness (2)
- functionalization (2)
- gait (2)
- gait analysis (2)
- galaxies (2)
- galaxies: high-redshift (2)
- gallbladder cancer (2)
- gamma rays: general (2)
- gamma-rays: general (2)
- gas phase electron spectroscopy (2)
- gate effect (2)
- gender stereotype trait (2)
- gene delivery (2)
- gene expression (2)
- generalized dissimilarity modelling (2)
- genetic differentiation (2)
- genetic diversity (2)
- genetic programming (2)
- genome-wide association (2)
- genotype (2)
- geochronology (2)
- geographic distribution (2)
- gestural organization (2)
- glacial maximum (2)
- global datasets (2)
- global mean temperature (2)
- global monsoon (2)
- global surface warming (2)
- global warming (2)
- global warming levels (2)
- glucocorticoid receptor (2)
- glycogen (2)
- gold nanoparticle assembly (2)
- government (2)
- gradient boosting (2)
- gradient flow (2)
- grafted polymers (2)
- grain-size distribution (2)
- granulite (2)
- graph transformation systems (2)
- green polymers (2)
- greenland shelf (2)
- grief (2)
- ground-motion modelling (2)
- groundwater inflow (2)
- groundwater vulnerability (2)
- growth restriction (2)
- gulf coastal plain (2)
- gwas (2)
- habit formation (2)
- habituation (2)
- habituation-switch paradigm (2)
- handheld device (2)
- hanging wall (2)
- hate speech detection (2)
- haute cuisine (2)
- health anxiety (2)
- healthy diets (2)
- heart failure (2)
- heat stress memory (2)
- heat-stress (2)
- heavy precipitation (2)
- heme (2)
- hemoglobin amount (2)
- heroism (2)
- heterojunction silicon solar cells (2)
- heuristics (2)
- hierarchy-of-hypotheses approach (2)
- high dimensional (2)
- high gastronomy (2)
- high risk (2)
- high-resolution record (2)
- higher education management (2)
- high‐risk drinking (2)
- holocene (2)
- honey bees (2)
- hormones (2)
- host adaptation (2)
- household analysis (2)
- hsp70 (2)
- human endotoxemia (2)
- human motion (2)
- human physical conditioning (2)
- human values (2)
- human-robot interaction (2)
- hybrid modelling (2)
- hydrological drought (2)
- hydrological models (2)
- hydrological signatures (2)
- hydroxyl-functional poly(2-vinyl pyridine) (2)
- hyperactivity (2)
- hyperbolic tilings (2)
- hyperthermia (2)
- ice absorption features (2)
- ice sheet (2)
- ideal-observer model (2)
- identifying influential nodes (2)
- identity (2)
- identity development (2)
- image processing (2)
- imaging (2)
- immune evasion cluster (2)
- immunogenicity (2)
- impact on pre-activated Achilles tendon (2)
- implicit bias (2)
- in-vitro-synthesis (2)
- indigene Völker (2)
- individual (2)
- individual effects (2)
- individual variation (2)
- induced pluripotent stem cells (2)
- industry 4.0 (2)
- inertial measurement unit (2)
- infant development (2)
- infant word learning (2)
- infection (2)
- infiltration (2)
- inflection (2)
- information (2)
- injury (2)
- injury risk (2)
- insights (2)
- institutional entrepreneurship (2)
- insulin receptor (2)
- insulin signaling (2)
- integrated flood risk management (2)
- integration (2)
- intelligence (2)
- intelligent tutoring system (ITS) (2)
- interaction (2)
- interactive technologies (2)
- interest (2)
- interfaces (2)
- interference control (2)
- internal load (2)
- internal structure (2)
- internalization (2)
- internalizing behavior (2)
- internationaler Handel (2)
- internet addiction (2)
- interpersonal relations (2)
- interstellar ice (2)
- intertextuality (2)
- invasibility (2)
- invasion success (2)
- inversion (2)
- ionic liquid (2)
- ionogel (2)
- island disharmony (2)
- island syndromes (2)
- isometric muscle action (2)
- isotopes (2)
- isotopic tiling theory (2)
- jasmonate (2)
- job (2)
- judgement accuracy (2)
- kernel density estimation (2)
- kettle hole (2)
- knowledge and movement (2)
- laboratory (2)
- lactate (2)
- lake Donggi Cona (2)
- lake drainage (2)
- lake periphyton (2)
- laminitis (2)
- land management (2)
- landing positions (2)
- landscape generator (2)
- landscape of fear (2)
- landscape transience (2)
- landslides (2)
- large-scale (2)
- last glacial maximum (2)
- last glacial period (2)
- late pleistocene (2)
- later health (2)
- le Patrimoine d’Alexander von Humboldt (2)
- learning factories (2)
- length measurements (2)
- level of personality functioning (2)
- leverage areas (2)
- levoglucosan (2)
- lifestyle (2)
- ligand exchange (2)
- light scattering (2)
- lignocellulosic biomass (2)
- line: profiles (2)
- linear sprint (2)
- lipidation (2)
- lipid–lipid interactions (2)
- lipoplexes (2)
- liquid-phase catalysis (2)
- liveness (2)
- localisation (2)
- location prediction algorithm (2)
- loci (2)
- logarithmic source condition (2)
- logistic regression (2)
- low (2)
- low-back pain (2)
- low-energy electrons (2)
- lower thermosphere (2)
- luminescence (2)
- lymphoma (2)
- magma-poor (2)
- maintenance (2)
- maintenance of genomic integrity (2)
- major depression (2)
- manual muscle testing (2)
- manuscrits (2)
- maps on surfaces (2)
- margins (2)
- marine terrace (2)
- mass (2)
- mass conservation (2)
- mass index (2)
- matching dependencies (2)
- mathematics (2)
- mathematics instruction (2)
- maximum and range (2)
- meal timing (2)
- mechanical strength (2)
- mechanical tendinous oscillations (2)
- mechanism (2)
- media (2)
- mediated delivery (2)
- medicine (2)
- melatonin (2)
- membrane biophysics (2)
- membrane fusion (2)
- membrane microdomains (2)
- membrane stabilization (2)
- mental disorders (2)
- mental number line (MNL) (2)
- mentalization (2)
- mercaptocarboxylic acids (2)
- mesenchymal stem cells (2)
- mesoscale (2)
- metabolomic (2)
- metabolomics (2)
- metagenome (2)
- metagenomic analysis (2)
- metal halide perovskites (2)
- metal species (2)
- metallic nanolattices (2)
- metaophiolite (2)
- meteoric 10Be (2)
- methane (2)
- methodology (2)
- methods: observational (2)
- microbial interactions (2)
- microcomputed tomography (2)
- microcontact printing (2)
- microcystin (2)
- micronutrients (2)
- microplastics (2)
- microsatellites (2)
- microscopy (2)
- middle adulthood (2)
- mild solution (2)
- minimal pairs (2)
- minority shareholdings (2)
- mitochondria (2)
- mixed methods (2)
- mobile eye-tracking (2)
- modified Landweber method (2)
- molecular architecture (2)
- molecular bottle brushes (2)
- molecular dynamics simulations (2)
- molecular phylogeny (2)
- molecular tracers (2)
- molecularly imprinted polymers (2)
- molybdenum (2)
- molybdopterin synthase (2)
- monomers (2)
- mood (2)
- morphological decomposition (2)
- morphological errors (2)
- motion capture (2)
- motor neurons (2)
- movement ecology (2)
- multi-slab (2)
- multidisciplinary pain treatment (2)
- multiplicative noise (2)
- multishell (2)
- multi‐ year flooding cycle (2)
- murchison meteorite (2)
- muscle oxygenation (2)
- muscle power (2)
- muscle strength (2)
- musicality (2)
- mutagenesis (2)
- n-oxide reductase (2)
- narcissism (2)
- national multiplication training (2)
- native American ancestry (2)
- natural climate solutions (2)
- natural language processing (2)
- natural-selection (2)
- naturalism (2)
- nature conservation (2)
- necrobiome (2)
- negation (2)
- nested vulnerabilities (2)
- neurodegenerative diseases (2)
- neuroendocrine (2)
- neuromuscular diagnostics (2)
- neuroscience (2)
- neutron (2)
- newborns (2)
- news media (2)
- nightlights (2)
- nitrogen (2)
- nitrogen limitation (2)
- non-Gaussianity (2)
- non-radiative interface recombination (2)
- nonfullerene acceptors (2)
- nonlinear operator (2)
- nonlinear waves (2)
- nonstationarity (2)
- nonumiform background configuration (2)
- nouvelle cuisine (2)
- novel ecosystems (2)
- novel species (2)
- nucleus (2)
- numerical development (2)
- numerical model (2)
- numerical simulations (2)
- nutrient transport (2)
- nutritional characteristics (2)
- ocean acidification (2)
- ocean heat uptake (2)
- oligocene climate (2)
- one-carbon metabolism (2)
- open materials (2)
- orchard management (2)
- organic aerosols (2)
- organic dye pigments (2)
- organic photovoltaics (2)
- organic search (2)
- organic solar cell (2)
- organic–inorganic hybrid (2)
- origination (2)
- orogeny (2)
- osmotic-pressure (2)
- osteoporosis (2)
- other-race effect (2)
- overhead athlete (2)
- overhunting (2)
- overreaching (2)
- overtraining (2)
- overweight (2)
- oxidative stress tolerance (2)
- pacific oyster (2)
- palaeogenomics (2)
- paleoenvironmental records (2)
- paleosol sequence (2)
- parafoveal and foveal processing (2)
- paralinguistic features (2)
- parameterization (2)
- parameters (2)
- paraquat (2)
- parent (2)
- parent–child relationship (2)
- part 1 (2)
- passive microwave (2)
- patholinguistics (2)
- pathways (2)
- pattern formation (2)
- people (2)
- peptides markers (2)
- perfluorocarbon emulsion (2)
- perialpine lakes (2)
- periplasmic nitrate reductase (2)
- permafrost degradation (2)
- permafrost regions (2)
- permafrost thaw (2)
- permeability (2)
- permeability test (2)
- perpetration (2)
- personality-traits (2)
- perspective-taking (2)
- pervasive healthcare (2)
- phagocytosis (2)
- phase purity (2)
- phase transfer (2)
- phase transition (2)
- phenotypic phase plane (2)
- phonological development (2)
- phosphatidylserine (2)
- phosphorylation (2)
- photo-isomerization kinetics (2)
- photo-sensitive surfactant (2)
- photocatalysis (2)
- photocurrent generation (2)
- photon recycling (2)
- photostability (2)
- photovoltaics (2)
- physical activity (2)
- physical fitness (2)
- physical performance (2)
- physiology (2)
- pickering emulsion (2)
- piezoelectric polymers (2)
- planetary-waves (2)
- planets and satellites: atmospheres (2)
- plankton (2)
- plant Mediator (2)
- plant clonality (2)
- plant macrofossil data (2)
- plant naturalization (2)
- plant–soil feedback (2)
- plasma (2)
- plasma membrane (2)
- playa (2)
- polarization (2)
- politeness (2)
- political ideology (2)
- pollen records (2)
- pollution (2)
- poly (acrylic acid, sodium salt) (2)
- poly(ADP-ribosyl)ation (2)
- polyacrylamide (2)
- polydimethylsiloxane wrinkles (2)
- polymer amphiphile (2)
- polymer degradation (2)
- polymersomes (2)
- polypropylene (2)
- poly‐ ε ‐ caprolactone (2)
- poor literacy (2)
- population-specific risk marker (2)
- porous carbon materials (2)
- postural sway (2)
- potential-functions (2)
- power spectrum (2)
- practice (2)
- pre-service teachers (2)
- preactivation (2)
- pregnancy (2)
- preparedness (2)
- prevention (2)
- principal (2)
- principal components (2)
- printing (2)
- private households (2)
- privileged ground (2)
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Similar Yet Different
(2020)
The importance of intrinsically disordered late embryogenesis abundant (LEA) proteins in the tolerance to abiotic stresses involving cellular dehydration is undisputed. While structural transitions of LEA proteins in response to changes in water availability are commonly observed and several molecular functions have been suggested, a systematic, comprehensive and comparative study of possible underlying sequence-structure-function relationships is still lacking. We performed molecular dynamics (MD) simulations as well as spectroscopic and light scattering experiments to characterize six members of two distinct, lowly homologous clades of LEA_4 family proteins from Arabidopsis thaliana. We compared structural and functional characteristics to elucidate to what degree structure and function are encoded in LEA protein sequences and complemented these findings with physicochemical properties identified in a systematic bioinformatics study of the entire Arabidopsis thaliana LEA_4 family. Our results demonstrate that although the six experimentally characterized LEA_4 proteins have similar structural and functional characteristics, differences concerning their folding propensity and membrane stabilization capacity during a freeze/thaw cycle are obvious. These differences cannot be easily attributed to sequence conservation, simple physicochemical characteristics or the abundance of sequence motifs. Moreover, the folding propensity does not appear to be correlated with membrane stabilization capacity. Therefore, the refinement of LEA_4 structural and functional properties is likely encoded in specific patterns of their physicochemical characteristics.
Der Band versammelt verschiedenen Beiträge, die sich mit dem Leben und Werk von Peter Weiss auseinandersetzen. Die Beiträge wurden auf der Konferenz "Peter Weiss - 100" anläßlich des hundertsten Geburtstages des Autors im Jahre 2016 in Potsdam gehalten. Dabei wirkten unterschiedliche Einrichtungen und Institutionen erstmals gemeinsam im Geburtsort des Künstlers.
Vorwort
(2020)
Reconstructing democracy
(2020)
Across the world, democracies are suffering from a disconnect between the people and political elites. In communities where jobs and industry are scarce, many feel the government is incapable of understanding their needs or addressing their problems. The resulting frustration has fueled the success of destabilizing demagogues. To reverse this pattern and restore responsible government, we need to reinvigorate democracy at the local level. But what does that mean? Drawing on examples of successful community building in cities large and small, from a shrinking village in rural Austria to a neglected section of San Diego, Reconstructing Democracy makes a powerful case for re-engaging citizens. It highlights innovative grassroots projects and shows how local activists can form alliances and discover their own power to solve problems.
During the phases of mobile warfare, the ethnically and religiously very heterogeneous population in the border regions of the multi-ethnic empires suffered in particular. Even if the real military situation in the course of the war hardly gave cause for concern, the image of disloyal ethnic and national minorities was widespread. This was particularly the case when ethnic groups lived on both sides of the border and social and political tensions had already established themselves along ethnic or religious lines of conflict before the war. Displacements, deportations and mass violence were the result. The genocide of the Armenian population is the most extreme example of this development. This anthology examines the border regions of the Ottoman, Russian and Habsburg empires during the First World War with regard to radical population policy and genocidal violence from a comparative perspective in order to draw a more precise picture of escalating and deescalating factors.
Background
Although associations between cardiovascular diseases and cognitive impairment are well known, the impact of cognitive performance on the success of patient education as a core component of cardiac rehabilitation remains insufficiently investigated so far.
Design
Prospective observational study in two inpatient cardiac rehabilitation centres between September 2014 and August 2015 with a follow-up six months after cardiac rehabilitation.
Method
At admission to and discharge from cardiac rehabilitation, the cognitive performance of 401 patients (54.5 ± 6.3 years, 80% men) following an acute coronary syndrome and/or coronary artery bypass graft was tested using the Montreal Cognitive Assessment. Patients’ disease-related knowledge was determined using a quiz (22 items for medical knowledge and 12 items for healthy lifestyle and behaviour) at both times and at follow-up. The change in knowledge after cardiac rehabilitation was analysed in multivariable regression models. Potentially influencing parameters (e.g. level of education, medication, cardiovascular risk factors, coronary artery bypass graft, comorbidities, exercise capacity) were considered.
Results
During cardiac rehabilitation, disease-related knowledge was significantly enhanced in both scales. At follow-up, the average level of medical knowledge was significantly reduced, while lifestyle knowledge remained at a stable level. The maintenance of knowledge after cardiac rehabilitation was predominantly predicted by prior knowledge, cognitive performance at discharge from cardiac rehabilitation and, in the case of medical knowledge, by coronary artery bypass graft.
Conclusion
Patient education in cardiac rehabilitation led to enhanced disease-related knowledge, but the maintenance of this essentially depended on patients’ cognitive performance, especially after coronary artery bypass graft. Therefore, patient education concepts in cardiac rehabilitation should be reconsidered and adjusted as needed.
Research synthesis on simple yet general hypotheses and ideas is challenging in scientific disciplines studying highly context-dependent systems such as medical, social, and biological sciences. This study shows that machine learning, equation-free statistical modeling of artificial intelligence, is a promising synthesis tool for discovering novel patterns and the source of controversy in a general hypothesis. We apply a decision tree algorithm, assuming that evidence from various contexts can be adequately integrated in a hierarchically nested structure. As a case study, we analyzed 163 articles that studied a prominent hypothesis in invasion biology, the enemy release hypothesis. We explored if any of the nine attributes that classify each study can differentiate conclusions as classification problem. Results corroborated that machine learning can be useful for research synthesis, as the algorithm could detect patterns that had been already focused in previous narrative reviews. Compared with the previous synthesis study that assessed the same evidence collection based on experts' judgement, the algorithm has newly proposed that the studies focusing on Asian regions mostly supported the hypothesis, suggesting that more detailed investigations in these regions can enhance our understanding of the hypothesis. We suggest that machine learning algorithms can be a promising synthesis tool especially where studies (a) reformulate a general hypothesis from different perspectives, (b) use different methods or variables, or (c) report insufficient information for conducting meta-analyses.
Flight behaviour characteristics such as flight altitude and avoidance behaviour determine the species-specific collision risk of birds with wind turbines. However, traditional observational methods exhibit limited positional accuracy. High-resolution GPS telemetry represents a promising method to overcome this drawback. In this study, we used three-dimensional GPS tracking data including high-accuracy tracks recorded at 3-s intervals to investigate the collision risk of breeding male Montagu's Harriers Circus pygargus in the Dutch–German border region. Avoidance of wind turbines was quantified by a novel approach comparing observed flights to a null model of random flight behaviour. On average, Montagu's Harriers spent as much as 8.2 h per day in flight. Most flights were at low altitude, with only 7.1% within the average rotor height range (RHR; 45–125 m). Montagu's Harriers showed significant avoidance behaviour, approaching turbines less often than expected, particularly when flying within the RHR (avoidance rate of 93.5%). For the present state, with wind farms situated on the fringes of the regional nesting range, collision risk models based on our new insights on flight behaviour indicated 0.6–2.0 yearly collisions of adult males (as compared with a population size of c. 40 pairs). However, the erection of a new wind farm inside the core breeding area could markedly increase mortality (up to 9.7 yearly collisions). If repowering of the wind farms was carried out using low-reaching modern turbines (RHR 36–150 m), mortality would more than double, whereas it would stay approximately constant if higher turbines (RHR 86–200 m) were used. Our study demonstrates the great potential of high-resolution GPS tracking for collision risk assessments. The resulting information on collision-related flight behaviour allows for performing detailed scenario analyses on wind farm siting and turbine design, in contrast to current environmental assessment practices. With regard to Montagu's Harriers, we conclude that although the deployment of higher wind turbines represents an opportunity to reduce collision risk for this species, precluding wind energy developments in core breeding areas remains the most important mitigation measure.
Anthropogenic activities have led to a global decline in biodiversity, and monitoring studies indicate that both insect communities and wetland ecosystems are particularly affected. However, there is a need for long-term data (over centennial or millennial timescales) to better understand natural community dynamics and the processes that govern the observed trends. Chironomids (Insecta: Diptera: Chironomidae) are often the most abundant insects in lake ecosystems, sensitive to environmental change, and, because their larval exoskeleton head capsules preserve well in lake sediments, they provide a unique record of insect community dynamics through time. Here, we provide the results of a metadata analysis of chironomid diversity across a range of spatial and temporal scales. First, we analyse spatial trends in chironomid diversity using Northern Hemispheric data sets overall consisting of 837 lakes. Our results indicate that in most of our data sets, summer temperature (T-jul) is strongly associated with spatial trends in modern-day chironomid diversity. We observe a strong increase in chironomid alpha diversity with increasing T-jul in regions with present-day T-jul between 2.5 and 14 degrees C. In some areas with T-jul > 14 degrees C, chironomid diversity stabilizes or declines. Second, we demonstrate that the direction and amplitude of change in alpha diversity in a compilation of subfossil chironomid records spanning the last glacial-interglacial transition (similar to 15,000-11,000 years ago) are similar to those observed in our modern data. A compilation of Holocene records shows that during phases when the amplitude of temperature change was small, site-specific factors had a greater influence on the chironomid fauna obscuring the chironomid diversity-temperature relationship. Our results imply expected overall chironomid diversity increases in colder regions such as the Arctic under sustained global warming, but with complex and not necessarily predictable responses for individual sites.
Successful societies
(2020)
Combining moral philosophy with sociological theory to build on themes introduced in Hall and Lamont’s Successful Societies (2009), the paper outlines a distinctive perspective. It holds that a necessary condition of successful societies is that decision-makers base their decisions on a high level of attentiveness (concern and comprehension) towards subjectively valued and morally legitimate forms of life. Late modern societies consist of a plurality of forms of life, each providing grounds for what Alasdair MacIntyre has called internal goods—valued and morally valuable practices. The status of such goods is examined, and distinctions are drawn between their manifest and latent, and transposable and situationally specific, characteristics. We integrate this refined idea of internal goods into a developed conception of habitus that is both morally informed and situationally embedded. The sociological approach of strong structuration theory (SST) is employed to demonstrate how this conception of habitus can guide the critique of decision-making that damages internal goods. We identify the most pervasive and invidious forms of damaging decision-making in contemporary societies as those involving excessive forms of instrumental reasoning. We argue that our developed conception of habitus, anchored in the collectively valued practices of specific worlds, can be a powerful focus for resistance. Accounts of scholarship in higher education and of the white working class in America illustrate the specificities of singular, particular, social worlds and illuminate critical challenges raised by the perspective we advocate.
Context Cities are a challenging habitat for obligate nocturnal mammals because of the ubiquitous use of artificial light at night (ALAN). How nocturnal animals move in an urban landscape, particularly in response to ALAN is largely unknown. Objectives We studied the movement responses, foraging and commuting, of common noctules (Nyctalus noctula) to urban landscape features in general and ALAN in particular. Methods We equipped 20 bats with miniaturized GPS loggers in the Berlin metropolitan area and related spatial positions of bats to anthropogenic and natural landscape features and levels of ALAN. Results Common noctules foraged close to ALAN only next to bodies of water or well vegetated areas, probably to exploit swarms of insects lured by street lights. In contrast, they avoided illuminated roads, irrespective of vegetation cover nearby. Predictive maps identified most of the metropolitan area as non-favoured by this species because of high levels of impervious surfaces and ALAN. Dark corridors were used by common noctules for commuting and thus likely improved the permeability of the city landscape. Conclusions We conclude that the spatial use of common noctules, previously considered to be more tolerant to light than other bats, is largely constrained by ALAN. Our study is the first individual-based GPS tracking study to show sensitive responses of nocturnal wildlife to light pollution. Approaches to protect urban biodiversity need to include ALAN to safeguard the larger network of dark habitats for bats and other nocturnal species in cities.
Einführung
(2020)
In the Middle Ages the European cities constituted the bourgeois laboratory for the formulation and the institutionalisation of the rights of citizenship. In 2014, the urban population accounted already for 54 per cent of global population. Yet, globalisation and neo-liberal policies have significantly challenged the social protection systems and social justice. From a sociological perspective, increased urbanisation implies a state of increased individual freedom, while at once it provokes growing social fragmentation. The chapter focuses on these dialectics and analyses to which degree social fragmentation affects the formal institutionalisation of citizenship rights and the substantial access to formally established rights, while at the same time excluding the most disadvantaged social groups, reducing them to mere ‘denizens’ of urban societies.
Permanent liminality?
(2020)
After more than eight years of multifaceted crisis and extreme austerity policies, the programmes of financial assistance to Greece ended in August 2018, only to be replaced by a strict surveillance plan which leaves little room for manoeuvre towards more socially sensitive policies. In this context, the paper focuses on three major issues of argument between the creditors and the Greek government: property ownership and taxation, reduction of pensions, and continued austerity. Based on research in Athens and starting from the premise that behind figures and statistics lie embodied subjects, the paper discusses these issues drawing from particular people’s experiences.
Introduction
(2020)
This introduction presents an overview of the key concepts discussed in the subsequent chapters of this book. The book considers the complex process of the criminalisation of squatting alongside and beyond its juridical apparatuses. It provides insights into the ways in which the substance of social citizenship has been rapidly and significantly weakened, so that life has become much more precarious for low-income urbanites as well as large parts of the middle-class. The book reconstructs the history of Berlin’s tenant referendum, which induced the passing of a new local Housing Provision Act—one of the most progressive pieces of housing legislation in Germany. It investigates the modes of doing citizenship and social rights in practice, which characterised the creation of Berlin’s Medibüro, a network of medical offices, where medical activists provide free medical services to residents lacking access to the medical insurance system, especially to illegal migrants. The book concludes by providing a brief outlook on future research on urban citizenship.
Matthias Walden
(2020)
Matthias Walden (1927–1984) war einer der Vertreter eines politischen Neuanfangs im Journalismus in Deutschland nach 1945. Im Kern seines politischen Denkens stand die Verteidigung der liberalen Demokratie, deren ideellen Gehalt Walden sowohl durch eine personelle Kontinuität in der Bundesrepublik Deutschland zum Nationalsozialismus als auch durch die von ihm als Anbiederung empfundene Neue Ostpolitik und den gesellschaftlichen Protest der 1960er und 1970er Jahre in Gefahr sah.
Als profilierter Leitartikler wurde er vor allem für den Verlag Axel Springer zu einem intellektuellen Impulsgeber. Walden war überzeugt, Diktaturen und totalitäre Gesellschaftsentwürfe sähen immer nur so aus, als wären sie für die Ewigkeit gemacht. Im Kalten Krieg gab ihm gerade die Unmenschlichkeit der kommunistischen Regime die Gewissheit, dass diese einst verschwinden würden.
Nils Lange legt mit seiner intellektuellen Biographie von Matthias Walden die erste umfassende Arbeit über diesen streitbaren Journalisten vor. Er arbeitet sowohl die politischen als auch die ideengeschichtlichen Ursprünge von Waldens Denken heraus.
Heterogenität
(2020)
‘The Territorialities of U.S. Imperialisms’ sets into relation U.S. imperial and Indigenous conceptions of territoriality as articulated in U.S. legal texts and Indigenous life writing in the 19th century. It analyzes the ways in which U.S. legal texts as “legal fictions” narratively press to affirm the United States’ territorial sovereignty and coherence in spite of its reliance on a variety of imperial practices that flexibly disconnect and (re)connect U.S. sovereignty, jurisdiction and territory.
At the same time, the book acknowledges Indigenous life writing as legal texts in their own right and with full juridical force, which aim to highlight the heterogeneity of U.S. national territory both from their individual perspectives and in conversation with these legal fictions. Through this, the book’s analysis contributes to a more nuanced understanding of the coloniality of U.S. legal fictions, while highlighting territoriality as a key concept in the fashioning of the narrative of U.S. imperialism.
Doppelte Zeitenwende
(2020)
The decision to exercise is not only bound to rational considerations but also automatic affective processes. The affective–reflective theory of physical inactivity and exercise (ART) proposes a theoretical framework for explaining how the automatic affective process (type‑1 process) will influence exercise behavior, i.e., through the automatic activation of exercise-related associations and a subsequent affective valuation of exercise. This study aimed to empirically test this assumption of the ART with data from 69 study participants. A single-measurement study, including within-subject experimental variation, was conducted. Automatic associations with exercise were first measured with a single-target implicit association test. The somato-affective core of the participants’ automatic valuation of exercise-related pictures was then assessed via heart rate variability (HRV) analysis, and the affective valence of the valuation was tested with a facial expression (FE; smile and frown) task. Exercise behavior was assessed via self-report. Multiple regression (path) analysis revealed that automatic associations predicted HRV reactivity (β = −0.24, p = .044); the signs of the correlation between automatic associations and the smile FE score was in the expected direction but remained nonsignificant (β = −0.21, p = .078). HRV reactivity predicted self-reported exercise behavior (β = −0.28, p = .013) (the same pattern of results was achieved for the frown FE score). The HRV-related results illustrate the potential role of automatic negative affective reactions to the thought of exercise as a restraining force in exercise motivation. For better empirical distinction between the two ART type‑1 process components, automatic associations and the affective valuation should perhaps be measured separately in the future. The results support the notion that automatic and affective processes should be regarded as essential aspects of the motivation to exercise.
Einführung
(2020)
To meet the demands of a growing world population while reducing carbon dioxide (CO2) emissions, it is necessary to capture CO2 and convert it into value-added compounds. In recent years, metabolic engineering of microbes has gained strong momentum as a strategy for the production of valuable chemicals. As common microbial feedstocks like glucose directly compete with human consumption, the one carbon (C1) compound formate was suggested as an alternative feedstock. Formate can be easily produced by various means including electrochemical reduction of CO2 and could serve as a feedstock for microbial production, hence presenting a novel entry point for CO2 to the biosphere and a storage option for excess electricity. Compared to the gaseous molecule CO2, formate is a highly soluble compound that can be easily handled and stored. It can serve as a carbon and energy source for natural formatotrophs, but these microbes are difficult to cultivate and engineer. In this work, I present the results of several projects that aim to establish efficient formatotrophic growth of E. coli – which cannot naturally grow on formate – via synthetic formate assimilation pathways. In the first study, I establish a workflow for growth-coupled metabolic engineering of E. coli. I demonstrate this approach by presenting an engineering scheme for the PFL-threonine cycle, a synthetic pathway for anaerobic formate assimilation in E. coli. The described methods are intended to create a standardized toolbox for engineers that aim to establish novel metabolic routes in E. coli and related organisms. The second chapter presents a study on the catalytic efficiency of C1-oxidizing enzymes in vivo. As formatotrophic growth requires generation of both energy and biomass from formate, the engineered E. coli strains need to be equipped with a highly efficient formate dehydrogenase, which provides reduction equivalents and ATP for formate assimilation. I engineered a strain that cannot generate reducing power and energy for cellular growth, when fed on acetate. Under this condition, the strain depends on the introduction of an enzymatic system for NADH regeneration, which could further produce ATP via oxidative phosphorylation. I show that the strain presents a valuable testing platform for C1-oxidizing enzymes by testing different NAD-dependent formate and methanol dehydrogenases in the energy auxotroph strain. Using this platform, several candidate enzymes with high in vivo activity, were identified and characterized as potential energy-generating systems for synthetic formatotrophic or methylotrophic growth in E. coli. In the third chapter, I present the establishment of the serine threonine cycle (STC) – a synthetic formate assimilation pathway – in E. coli. In this pathway, formate is assimilated via formate tetrahydrofolate ligase (FtfL) from Methylobacterium extorquens (M. extorquens). The carbon from formate is attached to glycine to produce serine, which is converted into pyruvate entering central metabolism. Via the natural threonine synthesis and cleavage route, glycine is regenerated and acetyl-CoA is produced as the pathway product. I engineered several selection strains that depend on different STC modules for growth and determined key enzymes that enable high flux through threonine synthesis and cleavage. I could show that expression of an auxiliary formate dehydrogenase was required to achieve growth via threonine synthesis and cleavage on pyruvate. By overexpressing most of the pathway enzymes from the genome, and applying adaptive laboratory evolution, growth on glycine and formate was achieved, indicating the activity of the complete cycle. The fourth chapter shows the establishment of the reductive glycine pathway (rGP) – a short, linear formate assimilation route – in E. coli. As in the STC, formate is assimilated via M. extorquens FtfL. The C1 from formate is condensed with CO2 via the reverse reaction of the glycine cleavage system to produce glycine. Another carbon from formate is attached to glycine to form serine, which is assimilated into central metabolism via pyruvate. The engineered E. coli strain, expressing most of the pathway genes from the genome, can grow via the rGP with formate or methanol as a sole carbon and energy source.
Das Ziel der vorliegenden Dissertation war es herauszuarbeiten, ob Nachhaltigkeitsbewusstsein den Konsum von Luxusgütern beeinflusst und ob verschiedene Moderatoren einen Einfluss auf diesen Zusammenhang ausüben. Das Nachhaltigkeitsbewusstsein wurde dabei basierend auf dem von Balderjahn et al. (2013) entwickelten Consciousness-for-sustainable-consumption-Modell durch die ökologische, soziale und die ökonomische Nachhaltigkeit sowie ergänzend durch das Tierschutzbewusstsein und Bewusstsein für lokale Produktion repräsentiert. Als Moderatoren dienten das Streben nach sozialer Anerkennung und Prestige, Materialismus, Hedonismus und Traditionsbewusstsein. Für die Aufdeckung möglicher Zusammenhänge zwischen den verschiedenen Dimensionen der Nachhaltigkeit und Luxuskonsum wurde eine Prädiktorenanalyse durchgeführt. Moderatorenanalysen offenbarten zusätzlich, ob ein Einfluss der verschiedenen Moderatoren auf die einzelnen Zusammenhänge vorlag. Die Untersuchung zeigte, dass jeweils das Umweltbewusstsein, das Bewusstsein für genügsamen Konsum sowie das Bewusstsein für schuldenfreien Konsum als Teil der ökonomischen Nachhaltigkeit und das Tierschutzbewusstsein einen Einfluss auf den Luxuskonsum ausüben. Darüber hinaus konnten insgesamt sieben Einflüsse durch die verschiedenen Moderatorvariablen auf die unterschiedlichen Zusammenhänge zwischen den Nachhaltigkeitsdimensionen und Luxuskonsum aufgedeckt werden.
With populations growing worldwide and climate change threatening food production there is an urgent need to find ways to ensure food security. Increasing carbon fixation rate in plants is a promising approach to boost crop yields. The carbon-fixing enzyme Rubisco catalyzes, beside the carboxylation reaction, also an oxygenation reaction that generates glycolate-2P, which needs to be recycled via a metabolic route termed photorespiration. Photorespiration dissipates energy and most importantly releases previously fixed CO2, thus significantly lowering carbon fixation rate and yield. Engineering plants to omit photorespiratory CO2 release is the goal of the FutureAgriculture consortium and this thesis is part of this collaboration. The consortium aims to establish alternative glycolate-2P recycling routes that do not release CO2. Ultimately, they are expected to increase carbon fixation rates and crop yields. Natural and novel reactions, which require enzyme engineering, were considered in the pathway design process. Here I describe the engineering of two pathways, the arabinose-5P and the erythrulose shunt. They were designed to recycle glycolate-2P via glycolaldehyde into a sugar phosphate and thereby reassimilate glycolate-2P to the Calvin cycle. I used Escherichia coli gene deletion strains to validate and characterize the activity of both synthetic shunts. The strains’ auxotrophies can be alleviated by the activity of the synthetic route, thus providing a direct way to select for pathway activity. I introduced all pathway components to these dedicated selection strains and discovered inhibitions, limitations and metabolic cross talk interfering with pathway activity. After resolving these issues, I was able to show the in vivo activity of all pathway components and combine them into functional modules.. Specifically, I demonstrate the activity of a new-to-nature module of glycolate reduction to glycolaldehyde. Also, I successfully show a new glycolaldehyde assimilation route via arabinose-5P to ribulose-5P. In addition, all necessary enzymes for glycolaldehyde assimilation via L-erythrulose were shown to be active and an L-threitol assimilation route via L-erythrulose was established in E. coli. On their own, these findings demonstrate the power of using an easily engineerable microbe to test novel pathways; combined, they will form the basis for implementing photorespiration bypasses in plants.
A surface modification of ultraflat gold nanotriangles (AuNTs) with different shaped nanoparticles is of special relevance for surface-enhanced Raman scattering (SERS) and the photo-catalytic activity of plasmonic substrates. Therefore, different approaches are used to verify the flat platelet morphology of the AuNTs by oriented overgrowth with metal nanoparticles. The most important part for the morphological transformation of the AuNTs is the coating layer, containing surfactants or polymers. By using well established AuNTs stabilized by a dioctyl sodium sulfosuccinate (AOT) bilayer, different strategies of surface modification with noble metal nanoparticles are possible. On the one hand undulated superstructures were synthesized by in situ growth of hemispherical gold nanoparticles in the polyethyleneimine (PEI)-coated AOT bilayer of the AuNTs. On the other hand spiked AuNTs were obtained by a direct reduction of Au³⁺ ions in the AOT double layer in presence of silver ions and ascorbic acid as reducing agent. Additionally, crumble topping of the smooth AuNTs can be realized after an exchange of the AOT bilayer by hyaluronic acid, followed by a silver-ion mediated reduction with ascorbic acid. Furthermore, a decoration with silver nanoparticles after coating the AOT bilayer with the cationic surfactant benzylhexadecyldimethylammonium chloride (BDAC) can be realized. In that case the ultraviolet (UV)-absorption of the undulated Au@Ag nanoplatelets can be tuned depending on the degree of decoration with silver nanoparticles. Comparing the Raman scattering data for the plasmon driven dimerization of 4-nitrothiophenol (4-NTP) to 4,4′-dimercaptoazobenzene (DMAB) one can conclude that the most important effect of surface modification with a 75 times higher enhancement factor in SERS experiments becomes available by decoration with gold spikes.
Die DDR im Jahr 1989
(2020)
Die Immediatzeitungsberichte der Potsdamer Regierungspräsidenten 1867-1914
Band 4., Registerband
(2020)
Der Klimawandel
(2020)
The game itself?
(2020)
In this paper, we reassess the notion and current state of ludohermeneutics in game studies, and propose a more solid foundation for how to conduct hermeneutic game analysis. We argue that there can be no ludo-hermeneutics as such, and that every game interpretation rests in a particular game ontology, whether implicit or explicit. The quality of this ontology, then, determines a vital aspect of the quality of the analysis.
Wind influences the development, architecture and morphology of plant roots and may modify subsequent interactions between plants and soil (plant–soil feedbacks—PSFs). However, information on wind effects on fine root morphology is scarce and the extent to which wind changes plant–soil interactions remains unclear. Therefore, we investigated the effects of two wind intensity levels by manipulating surrounding vegetation height in a grassland PSF field experiment. We grew four common plant species (two grasses and two non-leguminous forbs) with soil biota either previously conditioned by these or other species and tested the effect of wind on root:shoot ratio, fine root morphological traits as well as the outcome for PSFs. Wind intensity did not affect biomass allocation (i.e. root:shoot ratio) in any species. However, fine-root morphology of all species changed under high wind intensity. High wind intensity increased specific root length and surface area and decreased root tissue density, especially in the two grasses. Similarly, the direction of PSFs changed under high wind intensity in all four species, but differences in biomass production on the different soils between high and low wind intensity were marginal and most pronounced when comparing grasses with forbs. Because soils did not differ in plant-available nor total nutrient content, the results suggest that wind-induced changes in root morphology have the potential to influence plant–soil interactions. Linking wind-induced changes in fine-root morphology to effects on PSF improves our understanding of plant–soil interactions under changing environmental conditions.
Die als Digitalisierung bezeichnete umfassende gesellschaftliche bzw. kulturelle Transformation lässt auch das Grundgesetz nicht unberührt. Die Fortentwicklung der Lehre vom Verfassungswandel ermöglicht es, die verfassungsrechtlichen Dynamiken als einen strukturellen Verfassungswandel zu beschreiben, der sich am Leitbild digitaler Souveränität orientiert. Das Leitbild steht für die Verantwortung und die Legitimität des Staates unter den Bedingungen der Digitalisierung. Enrico Peuker entfaltet es exemplarisch in den verfassungsrechtlichen Kontexten der digitalen Infrastrukturen sowie der "digitalen" Grundrechte. Er plädiert für eine erweiterte Lesart der staatlichen Infrastrukturverantwortung als Optimierungsrecht hinsichtlich des Breitbandausbaus und der IT-Sicherheit und verortet die Grundrechtsentwicklungen in einem Drei-Stufen-Modell aus Interpretation, Innovation und Kodifikation.
Die Entwicklung einer Theorie zur schulischen Inklusion ist das zentrale Thema der Dissertation. Die Autorin nutzt empirische Analysen zur Umsetzung inklusiven Lernens sowie Daten zu sonderpädagogischen Förderschwerpunkten an inklusiven Grundschulen für die Erarbeitung von Bedingungen und Formen eines inklusiven Schulsystems. Empirische Daten zur Umsetzung inklusiver Bildung liegen aus vielen Bundesländern vor, es fehlte jedoch eine forschungsleitende Theorie zur Einordnung und Analyse der Daten. Jennifer Lambrecht hat diese Theorie auf Grundlage der Systemtheorie Luhmanns entwickelt. Sie differenziert zwischen Schulsystemen und verortet unterschiedliche Inklusionsverständnisse. Im Ergebnis entwickelt sie fünf Thesen zur schulischen Inklusion im allgemeinen Schulsystem und im Sonderschulsystem. Die Dissertation, die ein hochaktuelles Thema der empirischen Bildungsforschung behandelt, regt zum Mit- und Nachdenken an und generiert neue, interessante Forschungsfragen.
Permafrost is warming in the northern high latitudes, inducing highly dynamic thaw-related permafrost disturbances across the terrestrial Arctic. Monitoring and tracking of permafrost disturbances is important as they impact surrounding landscapes, ecosystems and infrastructure. Remote sensing provides the means to detect, map, and quantify these changes homogeneously across large regions and time scales. Existing Landsat-based algorithms assess different types of disturbances with similar spatiotemporal requirements. However, Landsat-based analyses are restricted in northern high latitudes due to the long repeat interval and frequent clouds, in particular at Arctic coastal sites. We therefore propose to combine Landsat and Sentinel-2 data for enhanced data coverage and present a combined annual mosaic workflow, expanding currently available algorithms, such as LandTrendr, to achieve more reliable time series analysis. We exemplary test the workflow for twelve sites across the northern high latitudes in Siberia. We assessed the number of images and cloud-free pixels, the spatial mosaic coverage and the mosaic quality with spectral comparisons. The number of available images increased steadily from 1999 to 2019 but especially from 2016 onward with the addition of Sentinel-2 images. Consequently, we have an increased number of cloud-free pixels even under challenging environmental conditions, which then serve as the input to the mosaicking process. In a comparison of annual mosaics, the Landsat+Sentinel-2 mosaics always fully covered the study areas (99.9–100 %), while Landsat-only mosaics contained data-gaps in the same years, only reaching coverage percentages of 27.2 %, 58.1 %, and 69.7 % for Sobo Sise, East Taymyr, and Kurungnakh in 2017, respectively. The spectral comparison of Landsat image, Sentinel-2 image, and Landsat+Sentinel-2 mosaic showed high correlation between the input images and mosaic bands (e.g., for Kurungnakh 0.91–0.97 between Landsat and Landsat+Sentinel-2 mosaic and 0.92–0.98 between Sentinel-2 and Landsat+Sentinel-2 mosaic) across all twelve study sites, testifying good quality mosaic results. Our results show that especially the results for northern, coastal areas was substantially improved with the Landsat+Sentinel-2 mosaics. By combining Landsat and Sentinel-2 data we accomplished to create reliably high spatial resolution input mosaics for time series analyses. Our approach allows to apply a high temporal continuous time series analysis to northern high latitude permafrost regions for the first time, overcoming substantial data gaps, and assess permafrost disturbance dynamics on an annual scale across large regions with algorithms such as LandTrendr by deriving the location, timing and progression of permafrost thaw disturbances
Engineering biotechnological microorganisms to use methanol as a feedstock for bioproduction is a major goal for the synthetic metabolism community. Here, we aim to redesign the natural serine cycle for implementation in E. coli. We propose the homoserine cycle, relying on two promiscuous formaldehyde aldolase reactions, as a superior pathway design. The homoserine cycle is expected to outperform the serine cycle and its variants with respect to biomass yield, thermodynamic favorability, and integration with host endogenous metabolism. Even as compared to the RuMP cycle, the most efficient naturally occurring methanol assimilation route, the homoserine cycle is expected to support higher yields of a wide array of products. We test the in vivo feasibility of the homoserine cycle by constructing several E. coli gene deletion strains whose growth is coupled to the activity of different pathway segments. Using this approach, we demonstrate that all required promiscuous enzymes are active enough to enable growth of the auxotrophic strains. Our findings thus identify a novel metabolic solution that opens the way to an optimized methylotrophic platform.
Formaldehyde is a highly reactive compound that participates in multiple spontaneous reactions, but these are mostly deleterious and damage cellular components. In contrast, the spontaneous condensation of formaldehyde with tetrahydrofolate (THF) has been proposed to contribute to the assimilation of this intermediate during growth on C1 carbon sources such as methanol. However, the in vivo rate of this condensation reaction is unknown and its possible contribution to growth remains elusive. Here, we used microbial platforms to assess the rate of this condensation in the cellular environment. We constructed Escherichia coli strains lacking the enzymes that naturally produce 5,10-methylene-THF. These strains were able to grow on minimal medium only when equipped with a sarcosine (N-methyl-glycine) oxidation pathway that sustained a high cellular concentration of formaldehyde, which spontaneously reacts with THF to produce 5,10-methylene-THF. We used flux balance analysis to derive the rate of the spontaneous condensation from the observed growth rate. According to this, we calculated that a microorganism obtaining its entire biomass via the spontaneous condensation of formaldehyde with THF would have a doubling time of more than three weeks. Hence, this spontaneous reaction is unlikely to serve as an effective route for formaldehyde assimilation.
Medientechnisches Wissen
(2020)
Das mehrbändige Lehrbuch vermittelt Studierenden und Medienwissenschaftlern eine Einführung aus den Naturwissenschaften, Ingenieurwissenschaften und Technik-Disziplinen. Dabei steht im Zentrum, die Lesekompetenz für die jeweilige Disziplin zu etablieren und zu verbessern, damit innerhalb medien- und kulturwissenschaftlicher Studienprogramme Fachtexte der einzelnen Disziplinen diskutiert werden können