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Through the reactions of 1-aminomethyl-2-naphthol and substituted 1-aminobenzyl-2-naphthols with 3,4-dihydroisoquinoline or 6,7-dimethoxy-3,4-dihydroisoquinoline under microwave conditions, naphth[1,2-e][1,3]oxazino[2,3-a]-isoquinoline derivatives were prepared in good yields. The latter reaction was extended by using 2-aminoarylmethyl-1-naphthols, leading to isomeric naphth-[2,1-e][1,3]oxazino[2,3-a] isoquinolines. Beside the detailed NMR spectroscopic and theoretical study of both stereochemistry and dynamic behaviour of these new conformational flexible heterocyclic ring systems an unexpected dynamic process between two diastereomers was observed in solution, studied by variable temperature H-1 NMR spectroscopy and the mechanism proved by theoretical DFT computations.
Trithiaazapentalene derivatives were prepared by the reaction of 2-alkylidene-4-oxothiazolidines with Lawesson's reagent. They are classified as two structurally different trithiaazapentalene compounds that have different contributions of monocyclic 1,2-dithiole and 1,2,4-dithiazole structures and degrees of aromaticity of the bicyclic trithiaazapentalene system. The electron-donating ability of substituents at the C(5) position of the trithiaazapentalene system is recognized as the main cause for changes in pi-Celectron distribution. This is the first complete study of substituent effects on the structure of trithiapentalenes. (C) 2013 Elsevier Ltd. All rights reserved.
A conformational study of N-acetyl glucosamine derivatives utilizing residual dipolar couplings
(2013)
The nature of the major steric substituent constant scales for alkyl substituents, i.e. Omega(S), E-R and E-S' scales, was studied with the aid of the NBO and the natural steric (STERIC) analyses. Cyclohexyl esters R-3-CCOOC6H11 (R = alkyl or H) were used as the model compounds. Special emphasis was laid on the potential contribution of the polar component in these steric substituent parameters. In the light of our model the Omega(S) scale seems to be dominantly a steric substituent constant scale as is seen on the strengths of the good correlation between the Omega(S) constants of the CR3 group and the total steric exchange energy values E-TSEE for the model compounds. However, the Omega(S) values also seem to include a minor electronic component due to the varying electrostatic effect via the C alpha atom. On the other hand, E-R and E-S' parameters largely hinge on the size dependent polar effect of the CR3 alkyl group. By way of our model this repulsive interaction can be quantified by descriptor Delta q(OCO), the natural charge difference q(C)(C=O) - Sigma qO for the O-C(=O) functional group. Delta q(OCO) depends on the E-TSEE values, on qC alpha and on the polarization coefficients of the oxygen hybrid in the NBO of the pi(C=O) bond. The size sensitivity of the kinetic E-S' constants can be connected to variation of the Burgi-Dunitz angle in the transition state for the standard reaction used. A comparison is made for the q(C)(C=O) or Delta q(OCO) values computed on the one hand with the NBO formalism and on the other hand with the Hirshfeld formalism. A practical novel substituent constant q(C)(C=O) for the size of the alkyl groups is introduced.
Laforin or malin deficiency causes Lafora disease, characterized by altered glycogen metabolism and teenage-onset neurodegeneration with intractable and invariably fatal epilepsy. Plant starches possess small amounts of metabolically essential monophosphate esters. Glycogen contains similar phosphate amounts, which are thought to originate from a glycogen synthase error side reaction and therefore lack any specific function. Glycogen is also believed to lack monophosphates at glucosyl carbon C6, an essential phosphorylation site in plant starch metabolism. We now show that glycogen phosphorylation is not due to a glycogen synthase side reaction, that C6 is a major glycogen phosphorylation site, and that C6 monophosphates predominate near centers of glycogen molecules and positively correlate with glycogen chain lengths. Laforin or malin deficiency causes C6 hyperphosphorylation, which results in malformed long-chained glycogen that accumulates in many tissues, causing neurodegeneration in brain. Our work advances the understanding of Lafora disease pathogenesis and suggests that glycogen phosphorylation has important metabolic function.
A series of 5-unsubstituted and 5-substituted 2-alkylidene-4-oxothiazolidine-S-oxides were synthesized by the sulfur-oxidation with m-CPBA. The stereochemistry of 5-substituted sulfoxides was determined by means of NMR spectroscopy and DFT theoretical calculations. It was found that the thermodynamically less stable anti-isomer was initially formed in the course of the oxidation, but it underwent epimerization to the mixture enriched in the more stable syn-isomer, during the work-up process. The higher stability of syn-isomers is ascribed to the stronger hyperconjugative sigma(C-H)->sigma*(S-O) interaction versus the weaker sigma(C-C)->sigma*(S-O) delocalization in their anti-counterparts and to the existence of intramolecular 1,5-CH center dot center dot center dot C hydrogen bonds.
Silacyclohexanes and silaheterocyclohexanes-why are they so different from other heterocyclohexanes?
(2013)
Stereochemical studies on silaheterocyclohexanes is a 'hot topic' as evidenced by the growing number of publications. During last 10 years a substantial number of substituted silacyclohexanes and heterocyclohexanes containing sulfur, oxygen or nitrogen as the second (or third) heteroatom have been synthesized and studied by variable temperature dynamic NMR spectroscopy, gas-phase electron diffraction, variable temperature IR, Raman, microwave spectroscopy with respect to thermodynamic (frozen conformational equilibria) and kinetic (barrier to ring inversion) information. As the stereochemistry of cyclohexane and its N-, O-, P-, S-hetero analogues is one of keystones of modern theoretical and synthetic organic and heterocyclic chemistry, the stereochemistry of silacyclohexane and its hetero analogs is an important element of theoretical and synthetic organosilicon chemistry. The various classes of saturated six-membered rings were critically compared and studied in detail with respect to differences in their stereochemistry and dynamic behavior.
The first Si-H-containing azasilaheterocycle, 1,3-dimethyl-3-silapiperidine 1, was synthesized, and its molecular structure and conformational properties were studied by gas-phase electron diffraction (GED), low temperature NMR, IR and Raman spectroscopy and quantum chemical calculations. The compound exists as a mixture of two conformers possessing the chair conformation with the equatorial NMe group and differing by axial or equatorial position of the SiMe group. In the gas phase, the SiMeax conformer predominates (GED: ax/eq = 65(7):35(7)%,Delta G = 0.36(18) kcal/mol; IR: ax/eq = 62(5):38(5)%,Delta G = 0.16(7) kcal/mol). In solution, at 143 k the SiMeeq conformer predominates' in the frozen equilibrium (NMR: ax/eq = 31.5(1.5):68.5(1.5)%, Delta G = -0.22(2) kcal/mol). Thermodynamic parameters of the ring inversion are determined (Delta G(double dagger) = 8.9-9.0 kcal/mol, Delta H-double dagger = 9.6 kcal/mol, Delta S-double dagger = 2.1 eu). High-level quantum chemical calculations :(MP2, G2, CCSD(T)) nicely reproduce the experimental geometry and the predominance of the axial conformer in the gas phase.
Push-pull allenes-conjugation, (anti)aromaticity and quantification of the push-pull character
(2013)
Structures, H-1/C-13 chemical shifts, and pi electron distribution/conjugation of an experimentally available and theoretically completed set of push-pull allenes Acc(2)C=C=CDon(2) (Acc=F, CHO, CF3, C N; Don=t-Bu, OMe, OEt, SMe, SEt, NCH2R) have been computed at the OFT level of theory. Both orthogonal linear and orthogonal bent structures have been obtained. In the latter case the push-pull character could be quantified by the quotient method. The C-13 chemical shift of the central allene carbon atom C-2 and chemical shift differences Delta delta(C-1, C-2) and Delta delta(C-2, C-3) of allene carbon atoms proved to be a quantitative alternative. TSNMRS of ring-closed push-pull allenes have been computed in addition and were employed to identify polar, carbene-like and carbone-like canonical structures of these molecules.
The spatial magnetic properties (Through-Space NMR Shieldings-TSNMRS) of already synthesized dehydro[n]annulenes of various ring size (from C-12 to C-20) have been computed, visualized as Isochemical Shielding Surfaces (ICSS) of various size and direction, and were examined subject to present (anti)aromaticity. For this purpose the thus quantified ring current effect of the macro cycles on proximate protons in proton NMR spectra was employed.
Selective ultrafast probing of transient hot chemisorbed and precursor States of CO on Ru(0001)
(2013)
We have studied the femtosecond dynamics following optical laser excitation of CO adsorbed on a Ru surface by monitoring changes in the occupied and unoccupied electronic structure using ultrafast soft x-ray absorption and emission. We recently reported [M. Dell'Angela et al. Science 339, 1302 (2013)] a phonon-mediated transition into a weakly adsorbed precursor state occurring on a time scale of >2 ps prior to desorption. Here we focus on processes within the first picosecond after laser excitation and show that the metal-adsorbate coordination is initially increased due to hot-electron-driven vibrational excitations. This process is faster than, but occurs in parallel with, the transition into the precursor state. With resonant x-ray emission spectroscopy, we probe each of these states selectively and determine the respective transient populations depending on optical laser fluence. Ab initio molecular dynamics simulations of CO adsorbed on Ru(0001) were performed at 1500 and 3000 K providing insight into the desorption process.
Resonant inelastic X-ray scattering and X-ray emission spectroscopy can be used to probe the energy and dispersion of the elementary low-energy excitations that govern functionality in matter: vibronic, charge, spin and orbital excitations(1-7). A key drawback of resonant inelastic X-ray scattering has been the need for high photon densities to compensate for fluorescence yields of less than a per cent for soft X-rays(8). Sample damage from the dominant non-radiative decays thus limits the materials to which such techniques can be applied and the spectral resolution that can be obtained. A means of improving the yield is therefore highly desirable. Here we demonstrate stimulated X-ray emission for crystalline silicon at photon densities that are easily achievable with free-electron lasers(9). The stimulated radiative decay of core excited species at the expense of non-radiative processes reduces sample damage and permits narrow-bandwidth detection in the directed beam of stimulated radiation. We deduce how stimulated X-ray emission can be enhanced by several orders of magnitude to provide, with high yield and reduced sample damage, a superior probe for low-energy excitations and their dispersion in matter. This is the first step to bringing nonlinear X-ray physics in the condensed phase from theory(10-16) to application.
A new matrix system for phosphorescent organic light-emitting diodes (OLEDs) based on an electron transporting component attached to an inert polymer backbone, an electronically neutral co-host, and a phosphorescent dye that serves as both emitter and hole conductor are presented. The inert co-host is used either as small molecules or covalently connected to the same chain as the electron-transporting host. The use of a small molecular inert co-host in the active layer is shown to be highly advantageous in comparison to a purely polymeric matrix bearing the same functionalities. Analysis of the dye phosphorescence decay in pure polymer, small molecular co-host film, and their blend lets to conclude that dye molecules distribute mostly in the small molecular co-host phase, where the co-host prevents agglomeration and self-quenching of the phosphorescence as well as energy transfer to the electron transporting units. In addition, the co-host accumulates at the anode interface where it acts as electron blocking layer and improves hole injection. This favorable phase separation between polymeric and small molecular components results in devices with efficiencies of about 47 cd/A at a luminance of 1000 cd/m(2). Investigation of OLED degradation demonstrates the presence of two time regimes: one fast component that leads to a strong decrease at short times followed by a slower decrease at longer times. Unlike the long time degradation, the efficiency loss that occurs at short times is reversible and can be recovered by annealing of the device at 180 degrees C. We also show that the long-time degradation must be related to a change of the optical and electrical bulk properties.
A diffractometer setup is presented, based on a laser-driven plasma X-ray source for reciprocal-space mapping with femtosecond temporal resolution. In order to map out the reciprocal space, an X-ray optic with a convergent beam is used with an X-ray area detector to detect symmetrically and asymmetrically diffracted X-ray photons simultaneously. The setup is particularly suited for measuring thin films or imperfect bulk samples with broad rocking curves. For quasi-perfect crystalline samples with insignificant in-plane Bragg peak broadening, the measured reciprocal-space maps can be corrected for the known resolution function of the diffractometer in order to achieve high-resolution rocking curves with improved data quality. In this case, the resolution of the diffractometer is not limited by the convergence of the incoming X-ray beam but is solely determined by its energy bandwidth.
Introduction: Gait speed is one of the most commonly and frequently used parameters to evaluate gait development. It is characterized by high variability when comparing different steps in children. The objective of this study was to determine intra-individual gait speed variability in children.
Methods: Gait speed measurements (6-10 trials across a 3 m walkway) were performed and analyzed in 8263 children, aged 1-15 years. The coefficient of variation (CV) served as a measure for intra-individual gait speed variability measured in 6.6 +/- 1.0 trials per child. Multiple linear regression analysis was conducted to evaluate the influence of age and body height on changes in variability. Additionally, a subgroup analysis for height within the group of 6-year-old children was applied.
Results: A successive reduction in gait speed variability (CV) was observed for age groups (age: 1-15 years) and body height groups (height: 0.70-1.90 m). The CV in the oldest subjects was only one third of the CV (CV 6.25 +/- 3.52%) in the youngest subjects (CV 16.58 +/- 10.01%). Up to the age of 8 years (or 1.40 m height) there was a significant reduction in CV over time, compared to a leveling off for the older (taller) children.
Discussion: The straightforward approach measuring gait speed variability in repeated trials might serve as a fundamental indicator for gait development in children. Walking velocity seems to increase to age 8. Enhanced gait speed consistency of repeated trials develops up to age 15.
In dynamic H-reflex measurements, the standardisation of the nerve stimulation to the gait cycle is crucial to avoid misinterpretation due to altered pre-synaptic inhibition. In this pilot study, a plantar pressure sole was used to trigger the stimulation of the tibialis nerve with respect to the gait cycle. Consequently, the intersession reliability of the soleus muscle H-reflex during treadmill walking was investigated.
Seven young participants performed walking trials on a treadmill at 5 km/h. The stimulating electrode was placed on the tibial nerve in the popliteal fossa. An EMG was recorded from the soleus muscle. To synchronize the stimulus to the gait cycle, initial heel strike was detected with a plantar pressure sole. Maximum H-reflex amplitude and M-wave amplitude were obtained and the Hmax/Mmax ratio was calculated.
Data reveals excellent reliability, ICC = 0.89. Test-retest variability was 13.0% (+/- 11.8). The Bland-Altman analysis showed a systematic error of 2.4%.
The plantar pressure sole was capable of triggering the stimulation of the tibialis nerve in a reliable way and offers a simple technique for the evaluation of reflex activity during walking.
How preview space/time translates into preview cost/benefit for fixation durations during reading
(2013)
Eye-movement control during reading depends on foveal and parafoveal information. If the parafoveal preview of the next word is suppressed, reading is less efficient. A linear mixed model (LMM) reanalysis of McDonald (2006) confirmed his observation that preview benefit may be limited to parafoveal words that have been selected as the saccade target. Going beyond the original analyses, in the same LMM, we examined how the preview effect (i.e., the difference in single-fixation duration, SFD, between random-letter and identical preview) depends on the gaze duration on the pretarget word and on the amplitude of the saccade moving the eye onto the target word. There were two key results: (a) The shorter the saccade amplitude (i.e., the larger preview space), the shorter a subsequent SFD with an identical preview; this association was not observed with a random-letter preview. (b) However, the longer the gaze duration on the pretarget word, the longer the subsequent SFD on the target, with the difference between random-letter string and identical previews increasing with preview time. A third patternincreasing cost of a random-letter string in the parafovea associated with shorter saccade amplitudeswas observed for target gaze durations. Thus, LMMs revealed that preview effects, which are typically summarized under preview benefit, are a complex mixture of preview cost and preview benefit and vary with preview space and preview time. The consequence for reading is that parafoveal preview may not only facilitate, but also interfere with lexical access.
This study aims to establish, evaluate, and apply a modern pollen-climate transfer function from the transition zone between arctic tundra and light-needled taiga in Arctic Siberia. Lacustrine samples (n = 96) from the northern Siberian lowlands of Yakutia were collected along four north-to-south transects crossing the arctic forest line. Samples span a broad temperature and precipitation gradient (mean July temperature, T-July: 7.5-18.7 degrees C; mean annual precipitation, P-ann: 114-315 mm/yr). Redundancy analyses are used to examine the relationship between the modern pollen signal and corresponding vegetation types and climate. Performance of transfer functions for T-July and P-ann were cross-validated and tested for spatial autocorrelation effects. The root mean square errors of prediction are 1.67 degrees C for T-July and 40 mm/yr for P-ann. A climate reconstruction based on fossil pollen spectra from a Siberian Arctic lake sediment core spanning the Holocene yielded cold conditions for the Late Glacial (1-2 degrees C below present T-July). Warm and moist conditions were reconstructed for the early to mid Holocene (2 degrees C higher T-July than present), and climate conditions similar to modern ones were reconstructed for the last 4000 years. In conclusion, our modern pollen data set fills the gap of existing regional calibration sets with regard to the underrepresented Siberian tundra-taiga transition zone. The Holocene climate reconstruction indicates that the temperature deviation from modern values was only moderate despite the assumed Arctic sensitivity to present climate change.
Microorganisms such as Bacteria and Archaea play important roles in the Arctic food web and biogeochemical cycles. Nevertheless, knowledge of microbial community composition in Greenland waters is scarce, and information on microorganisms associated with Arctic zooplankton species is virtually non-existent. We compared free-living microbial communities with those associated with two key copepod species (Calanus finmarchicus and Metridia longa) along a salinity gradient from the deep waters beyond Fyllas Banke to the inner part of Godthabsfjord, West Greenland, in summer 2008. Using genetic fingerprinting we found that free-living Bacteria (in particular Alphaproteobacteria) and Archaea varied with environmental factors and formed different communities along the fjord. Microbial communities associated with the two copepod species were clearly different from those in the ambient water. Surprisingly, Archaea could not be detected on the copepods. Our results show that zooplankton form "microbial islands" in the Arctic pelagic realm with a distinctive community composition and presumably functionality different from the free-living Bacteria. Changes in intensity and timing of meltwater runoff due to global warming are expected to affect these microbial assemblages differently, with potentially significant ramifications for Arctic food webs and biogeochemistry.
Siberian larch forests and the ion content of thaw lakes form a geochemically functional entity
(2013)
Siberian larch forests growing on shallow permafrost soils have not, until now, been considered to be controlling the abiotic and biotic characteristics of the vast number of thaw-lake ecosystems. Here we show, using four independent data sets (a modern data set from 201 lakes from the tundra to taiga, and three lake-core records), that lake-water geochemistry in Yakutia is highly correlated with vegetation. Alkalinity increases with catchment forest density. We postulate that in this arid area, higher evapotranspiration in larch forests compared with that in the tundra vegetation leads to local salt accumulation in soils. Solutes are transported to nearby thaw lakes during rain events and snow melt, but are not fully transported into rivers, because there is no continuous groundwater flow within permafrost soils. This implies that potentially large shifts in the chemical characteristics of aquatic ecosystems to known warming are absent because of the slow response of catchment forests to climate change.
The spatial and temporal variability of a low-centred polygon on the eastern floodplain area of the lower Anabar River (72.070 degrees N, 113.921 degrees E; northern Yakutia, Siberia) has been investigated using a multi-method approach. The present-day vegetation in each square metre was analysed, revealing a community of Larix, shrubby Betula, and Salix on the polygon rim, a dominance of Carex and Andromeda polifolia in the rim-to-pond transition zone, and a predominantly monospecific Scorpidium scorpioides coverage within the pond. The total organic carbon (TOC) content, TOC/TN (total nitrogen) ratio, grain size, vascular plant macrofossils, moss remains, diatoms, and pollen were analysed for two vertical sections and a sediment core from a transect across the polygon. Radiocarbon dating indicates that the formation of the polygon started at least 1500 yr ago; the general positions of the pond and rim have not changed since that time. Two types of pond vegetation were identified, indicating two contrasting development stages of the polygon. The first was a well-established moss association, dominated by submerged or floating Scorpidium scorpioides and/or Drepanocladus spp. and overgrown by epiphytic diatoms such as Tabellaria flocculosa and Eunotia taxa. This stage coincides temporally with a period in which the polygon was only drained by lateral subsurface water flow, as indicated by mixed grain sizes. A different moss association occurred during times of repeated river flooding (indicated by homogeneous medium-grained sand that probably accumulated during the annual spring snowmelt), characterized by an abundance of Meesia triquetra and a dominance of benthic diatoms (e. g. Navicula vulpina), indicative of a relatively high pH and a high tolerance of disturbance. A comparison of the local polygon vegetation (inferred from moss and macrofossil spectra) with the regional vegetation (inferred from pollen spectra) indicated that the moss association with Scorpidium scorpioides became established during relatively favourable climatic conditions, while the association dominated by Meesia triquetra occurred during periods of harsh climatic conditions. Our study revealed a strong riverine influence (in addition to climatic influences) on polygon development and the type of peat accumulated.
A high resolution multi proxy (pollen, grain size, total organic carbon) record from a small mountain lake (Lake Khuisiin; 46.6 degrees N, 101.8 degrees E; 2270 m a.s.l.) in the south eastern Khangai Mountains of central Mongolia has been used to explore changes in vegetation and climate over the last 1200 years. The pollen data indicates that the vegetation changed from dry steppe dominated by Poaceae and Artemisia (ca AD 760-950), to Larix forest steppe (ca AD 950-1170), Larix Betula forest steppe (ca AD 1170-1380), meadow dominated by Cyperaceae and Poaceae (ca AD 1380-1830), and Larix Betula forest steppe (after similar to AD 1830). The cold-wet period between AD 1380 and 1830 may relate to the Little Ice Age. Environmental changes were generally subtle and climate change seems to have been the major driver of variations in vegetation until at least the early part of the 20th century, suggesting that either the level of human activity was generally low, or the relationship between human activity and vegetation did not alter substantially between AD 760 and 1830. A review of centennial scale moisture records from China and Mongolia revealed that most areas experienced major changes at ca AD 1500 and AD 1900. However, the moisture availability since AD 1500 varied between sites, with no clear regional pattern or relationship to present day conditions. Both the reconstructions and the moisture levels simulation on a millennium scale performed in the MPI Earth System Model indicate that the monsoon-westerlies transition area shows a greater climate variability than those areas influenced by the westerlies, or by the summer monsoon only.
This study provides a detailed analysis of the mid-Holocene to present-day precipitation change in the Asian monsoon region. We compare for the first time results of high resolution climate model simulations with a standardised set of mid-Holocene moisture reconstructions. Changes in the simulated summer monsoon characteristics (onset, withdrawal, length and associated rainfall) and the mechanisms causing the Holocene precipitation changes are investigated. According to the model, most parts of the Indian subcontinent received more precipitation (up to 5 mm/day) at mid-Holocene than at present-day. This is related to a stronger Indian summer monsoon accompanied by an intensified vertically integrated moisture flux convergence. The East Asian monsoon region exhibits local inhomogeneities in the simulated annual precipitation signal. The sign of this signal depends on the balance of decreased pre-monsoon and increased monsoon precipitation at mid-Holocene compared to present-day. Hence, rainfall changes in the East Asian monsoon domain are not solely associated with modifications in the summer monsoon circulation but also depend on changes in the mid-latitudinal westerly wind system that dominates the circulation during the pre-monsoon season. The proxy-based climate reconstructions confirm the regional dissimilarities in the annual precipitation signal and agree well with the model results. Our results highlight the importance of including the pre-monsoon season in climate studies of the Asian monsoon system and point out the complex response of this system to the Holocene insolation forcing. The comparison with a coarse climate model simulation reveals that this complex response can only be resolved in high resolution simulations.
A total of 271 pollen records were selected from a large collection of both raw and digitized pollen spectra from eastern continental Asia (70 degrees-135 degrees E and 18 degrees-55 degrees N). Following pollen percentage recalculations, taxonomic homogenization, and age-depth model revision, the pollen spectra were interpolated at a 500-year resolution and a taxonomically harmonized and temporally standardized fossil pollen dataset established with 226 pollen taxa, covering the last 22 cal lea. Of the 271 pollen records, 85% were published since 1990, with reliable chronologies and high temporal resolutions; of these, 50% have raw data with complete pollen assemblages, ensuring the quality of this dataset The pollen records available for each 500-year time slice are well distributed over all main vegetation types and climatic zones of the study area, making their pollen spectra suitable for paleovegetation and paleoclimate research. Such a dataset can be used as an example for the development of similar datasets for other regions of the world.
In many hydrological applications, ground-wave velocity measurements are increasingly used to map and monitor shallow soil water content. In this study, we propose an automated spectral velocity analysis method to determine the direct ground-wave (DGW) velocity from common midpoint (CMP) or multi-offset ground-penetrating radar (GPR) data. The method introduced in this paper is a variation of the well-known spectral velocity analysis for seismic and GPR reflection events where velocity spectra are computed using different coherency measures along hyperbolas following the normal moveout model. Here, the unnormalized cross-correlation is computed between waveforms across data gathers that are corrected with a linear moveout equation using a predefined range of velocities. Peaks in the resulting velocity spectra identify linear events in the GPR data gathers like DGW events and allow for estimating the corresponding velocities. In addition to obtaining a DGW velocity measurement, we propose a robust method to estimate the associated velocity uncertainties based on the width of the peak in the calculated velocity spectrum. Our proposed method is tested on synthetic data examples to evaluate the influence of subsurface velocity, surveying geometry and signal frequency on the accuracy of estimated ground-wave velocities. In addition, we investigate the influence of such velocity uncertainties on subsequent soil water content estimates using an established petrophysical relationship. Furthermore, we apply our approach to analyse field data, which were collected across a test site in Canada to monitor a wide range of seasonal soil moisture variations. A comparison between our spectral velocity estimates and results derived from manually picked ground-wave arrivals shows good agreement, which illustrates that our spectral velocity analysis is a feasible tool to analyse DGW arrivals in multi-offset GPR data gathers in an objective and more automated manner.
In recent years, an increasing number of mutations in what would appear to be 'housekeeping genes' have been identified as having unexpectedly specific defects in multicellular organogenesis. This is also the case for organogenesis in seed plants. Although it is not surprising that loss-of-function mutations in 'housekeeping' genes result in lethality or growth retardation, it is surprising when (1) the mutant phenotype results from the loss of function of a 'housekeeping' gene and (2) the mutant phenotype is specific. In this review, by defining housekeeping genes as those encoding proteins that work in basic metabolic and cellular functions, we discuss unexpected links between housekeeping genes and specific developmental processes. In a surprising number of cases housekeeping genes coding for enzymes or proteins with functions in basic cellular processes such as transcription, post-transcriptional modification, and translation affect plant development.
We synthesize sub-THz longitudinal quasimonochromatic acoustic phonons in a SrTiO3 single crystal using a SrRuO3/SrTiO3 superlattice as an optical-acoustic transducer. The generated acoustic phonon spectrum is determined using ultrafast x-ray diffraction. The analysis of the generated phonon spectrum in the time domain reveals a k-vector dependent phonon lifetime. It is observed that even at sub-THz frequencies the phonon lifetime agrees with the 1/omega(2) power law known from Akhiezer's model for hyper sound attenuation. The observed shift of the synthesized spectrum to the higher q is discussed in the framework of nonlinear effects appearing due to the high amplitude of the synthesized phonons.
We measured Chinese dyslexic and control children's eye movements during rapid automatized naming (RAN) with alphanumeric (digits) and symbolic (dice surfaces) stimuli. Both types of stimuli required identical oral responses, controlling for effects associated with speech production. Results showed that naming dice was much slower than naming digits for both groups, but group differences in eye-movement measures and in the eye-voice span (i.e. the distance between the currently fixated item and the voiced item) were generally larger in digit-RAN than in dice-RAN. In addition, dyslexics were less efficient in parafoveal processing in these RAN tasks. Since the two RAN tasks required the same phonological output and on the assumption that naming dice is less practiced than naming digits in general, the results suggest that the translation of alphanumeric visual symbols into phonological codes is less efficient in dyslexic children. The dissociation of the print-to-sound conversion and phonological representation suggests that the degree of automaticity in translation from visual symbols to phonological codes in addition to phonological processing per se is also critical to understanding dyslexia.
It is well established that fixation durations during reading vary with processing difficulty, but there are different views on how oculomotor control, visual perception, shifts of attention, and lexical (and higher cognitive) processing are coordinated. Evidence for a one-to-one translation of input delay into saccadic latency would provide a much needed constraint for current theoretical proposals. Here, we tested predictions of such a direct-control perspective using the stimulus-onset delay (SOD) paradigm. Words in sentences were initially masked and, on fixation, were individually unmasked with a delay (0-, 33-, 66-, 99-ms SODs). In Experiment 1, SODs were constant for all words in a sentence; in Experiment 2, SODs were manipulated on target words, while nontargets were unmasked without delay. In accordance with predictions of direct control, nonzero SODs entailed equivalent increases in fixation durations in both experiments. Yet, a population of short fixations pointed to rapid saccades as a consequence of low-level information at nonoptimal viewing positions rather than of lexical processing. Implications of these results for theoretical accounts of oculomotor control are discussed.
We tested the limits of working-memory capacity (WMC) of young adults, old adults, and children with a memory-updating task. The task consisted of mentally shifting spatial positions within a grid according to arrows, their color signaling either only go (control) or go/no-go conditions. The interference model (IM) of Oberauer and Kliegl (2006) was simultaneously fitted to the data of all groups. In addition to the 3 main model parameters (feature overlap, noise, and processing rate), we estimated the time for switching between go and no-go steps as a new model parameter. In this study, we examined the IM parameters across the life span. The IM parameter estimates show that (a) conditions were not different in interference by feature overlap and interference by confusion; (b) switching costs time; (c) young adults and children were less susceptible than old adults to interference due to feature overlap; (d) noise was highest for children, followed by old and young adults; (e) old adults differed from children and young adults in lower processing rate; and (f) children and old adults had a larger switch cost between go steps and no-go steps. Thus, the results of this study indicated that across age, the IM parameters contribute distinctively for explaining the limits of WMC.
Additive and interactive effects of word frequency, stimulus quality, and semantic priming have been used to test theoretical claims about the cognitive architecture of word-reading processes. Additive effects among these factors have been taken as evidence for discrete-stage models of word reading. We present evidence from linear mixed-model analyses applied to 2 lexical decision experiments indicating that apparent additive effects can be the product of aggregating over- and underadditive interaction effects that are modulated by recent trial history, particularly the lexical status and stimulus quality of the previous trial's target. Even a simple practice effect expressed as improved response speed across trials was powerfully modulated by the nature of the previous target item. These results suggest that additivity and interaction between factors may reflect trial-to-trial variation in stimulus representations and decision processes rather than fundamental differences in processing architecture.
Dyslexic children are known to be slower than normal readers in rapid automatized naming (RAN). This suggests that dyslexics encounter local processing difficulties, which presumably induce a narrower perceptual span. Consequently, dyslexics should suffer less than normal readers from removing parafoveal preview. Here we used a gaze-contingent moving window paradigm in a RAN task to experimentally test this prediction. Results indicate that dyslexics extract less parafoveal information than control children. We propose that more attentional resources are recruited to the foveal processing because of dyslexics' less automatized translation of visual symbols into phonological output, thereby causing a reduction of the perceptual span. This in turn leads to less efficient preactivation of parafoveal information and, hence, more difficulty in processing the next foveal item.
Parafoveal semantic processing has recently been well documented in reading Chinese sentences, presumably because of language-specific features. However, because of a large variation of fixation landing positions on pretarget words, some preview words actually were located in foveal vision when readers' eyes landed close to the end of the pretarget words. None of the previous studies has completely ruled out a possibility that the semantic preview effects might mainly arise from these foveally processed preview words. This case, whether previously observed positive evidence for parafoveal semantic processing can still hold, has been called into question. Using linear mixed models, we demonstrate in this study that semantic preview benefit from word N+1 decreased if fixation on pretarget word N was close to the preview. We argue that parafoveal semantic processing is not a consequence of foveally processed preview words.
We explore the interaction between oculomotor control and language comprehension on the sentence level using two well-tested computational accounts of parsing difficulty. Previous work (Boston, Hale, Vasishth, & Kliegl, 2011) has shown that surprisal (Hale, 2001; Levy, 2008) and cue-based memory retrieval (Lewis & Vasishth, 2005) are significant and complementary predictors of reading time in an eyetracking corpus. It remains an open question how the sentence processor interacts with oculomotor control. Using a simple linking hypothesis proposed in Reichle, Warren, and McConnell (2009), we integrated both measures with the eye movement model EMMA (Salvucci, 2001) inside the cognitive architecture ACT-R (Anderson et al., 2004). We built a reading model that could initiate short Time Out regressions (Mitchell, Shen, Green, & Hodgson, 2008) that compensate for slow postlexical processing. This simple interaction enabled the model to predict the re-reading of words based on parsing difficulty. The model was evaluated in different configurations on the prediction of frequency effects on the Potsdam Sentence Corpus. The extension of EMMA with postlexical processing improved its predictions and reproduced re-reading rates and durations with a reasonable fit to the data. This demonstration, based on simple and independently motivated assumptions, serves as a foundational step toward a precise investigation of the interaction between high-level language processing and eye movement control.
Primary saccades are often followed by small secondary saccades, which are generally thought to reduce the distance between the saccade endpoint and target location. Accumulated evidence demonstrates that secondary saccades are subject to various influences, among which retinal feedback during postsaccadic fixation constitutes only one important signal. Recently, we reported that target eccentricity and an orientation bias influence the generation of secondary saccades. In the present study, we examine secondary saccades in the absence of postsaccadic visual feedback. Although extraretinal signals (e.g., efference copy) have received widespread attention in eye-movement studies, it is still unclear whether an extraretinal error signal contributes to the programming of secondary saccades. We have observed that secondary saccade latency and amplitude depend on primary saccade error despite the absence of postsaccadic visual feedback. Strong evidence for an extraretinal error signal influencing secondary saccade programming is given by the observation that secondary saccades are more likely to be oriented in a direction opposite to the primary saccade as primary saccade error shifts from target undershoot to overshoot. We further show how the functional relationship between primary saccade landing position and secondary saccade characteristics varies as a function of target eccentricity. We propose that initial target eccentricity and an extraretinal error signal codetermine the postsaccadic activity distribution in the saccadic motor map when no visual feedback is available.
Replicability of findings is at the heart of any empirical science. The aim of this article is to move the current replicability debate in psychology towards concrete recommendations for improvement. We focus on research practices but also offer guidelines for reviewers, editors, journal management, teachers, granting institutions, and university promotion committees, highlighting some of the emerging and existing practical solutions that can facilitate implementation of these recommendations. The challenges for improving replicability in psychological science are systemic. Improvement can occur only if changes are made at many levels of practice, evaluation, and reward.
Task demands and individual differences have been linked reliably to word skipping during reading. Such differences in fixation probability may imply a selection effect for multivariate analyses of eye-movement corpora if selection effects correlate with word properties of skipped words. For example, with fewer fixations on short and highly frequent words the power to detect parafoveal-on-foveal effects is reduced. We demonstrate that increasing the fixation probability on function words with a manipulation of the expected difficulty and frequency of questions reduces an age difference in skipping probability (i.e., old adults become comparable to young adults) and helps to uncover significant parafoveal-on-foveal effects in this group of old adults. We discuss implications for the comparison of results of eye-movement research based on multivariate analysis of corpus data with those from display-contingent manipulations of target words.
Haberlea rhodopensis is a resurrection species with extreme resistance to drought stress and desiccation but also with ability to withstand low temperatures and freezing stress. In order to identify biochemical strategies which contribute to Haberlea's remarkable stress tolerance, the metabolic reconfiguration of H. rhodopensis during low temperature (4 degrees C) and subsequent return to optimal temperatures (21 degrees C) was investigated and compared with that of the stress tolerant Thellungiella halophyla and the stress sensitive Arabidopsis thaliana. Metabolic analysis by GC-MS revealed intrinsic differences in the metabolite levels of the three species even at 21 degrees C. H. rhodopensis had significantly more raffinose, melibiose, trehalose, rhamnose, myo-inositol, sorbitol, galactinol, erythronate, threonate, 2-oxoglutarate, citrate, and glycerol than the other two species. A. thaliana had the highest levels of putrescine and fumarate, while T halophila had much higher levels of several amino acids, including alanine, asparagine, beta-alanine, histidine, isoleucine, phenylalanine, serine, threonine, and valine. In addition, the three species responded differently to the low temperature treatment and the subsequent recovery, especially with regard to the sugar metabolism. Chilling induced accumulation of maltose in H. rhodopensis and raffinose in A. thaliana but the raffinose levels in low temperature exposed Arabidopsis were still much lower than these in unstressed Haberlea. While all species accumulated sucrose during chilling, that accumulation was transient in H. rhodopensis and A. thaliana but sustained in T halophila after the return to optimal temperature. Thus, Haberlea's metabolome appeared primed for chilling stress but the low temperature acclimation induced additional stress-protective mechanisms. A diverse array of sugars, organic acids, and polyols constitute Haberlea's main metabolic defence mechanisms against chilling, while accumulation of amino acids and amino acid derivatives contribute to the low temperature acclimation in Arabidopsis and Thellungiella. Collectively, these results show inherent differences in the metabolomes under the ambient temperature and the strategies to respond to low temperature in the three species.
Objective: To test the effect of a complex guideline-based intervention on agitation and psychotropic prescriptions.
Design, Setting, Participants: Cluster randomized controlled trial (VIDEANT) with blinded assessment of outcome in 18 nursing homes in Berlin, Germany, comprising 304 dementia patients.
Intervention: Training, support, and activity therapy intervention, delivered at the level of each nursing home, focusing on the management of agitation in dementia. Control group nursing homes received treatment as usual.
Measurements: Levels of agitated and disruptive behavior (Cohen-Mansfield agitation inventory [CMAI]) as the primary outcome. Number of neuroleptics, antidepressants, and cholinesterase inhibitors (ChEIs) prescribed in defined daily dosages (DDDs).
Results: Of 326 patients screened, 304 (93.3%) were eligible and cluster-randomized to 9 intervention (n = 163) and 9 control (n = 141) nursing homes. Data were collected from 287 (94.4%) patients at 10 months. At 10 months, compared with controls, nursing home residents with dementia in the intervention group exhibited significantly less agitation as measured with the CMAI (adjusted mean difference, 6.24; 95% CI 2.03-14.14; P = .009; Cohen's d = 0.43), received fewer neuroleptics (P < .05), more ChEIs (P < .05), and more antidepressants (P < .05).
Conclusion: Complex guideline-based interventions are effective in reducing agitated and disruptive behavior in nursing home residents with dementia. At the same time, increased prescription of ChEIs and antidepressants together with decreased neuroleptic prescription suggests an effect toward guideline-based pharmacotherapy.
Objectives: To investigate the efficacy of animal-assisted therapy (AAT) on symptoms of agitation/aggression and depression in nursing home residents with dementia in a randomized controlled trial. Previous studies have indicated that AAT has beneficial effects on neuropsychiatric symptoms in various psychiatric disorders but few studies have investigated the efficacy of AAT in patients suffering from dementia. Methods: Of 65 nursing home residents with dementia (mean [standard deviation] age: 81.8 [9.2] years; mean Mini-Mental State Examination score: 7.1 [0.7]), 27 matched pairs (N = 54) were randomly assigned to either treatment as usual or treatment as usual combined with AAT, administered over 10 weekly sessions. Blinded raters assessed cognitive impairment with the Mini-Mental State Examination, presence of agitation/aggression with the Cohen-Mansfield Agitation Inventory, and depression with the Dementia Mood Assessment Scale at baseline and during a period of 4 weeks after AAT intervention. Results: In the control group, symptoms of agitation/aggression and depression significantly increased over 10 weeks; in the intervention group, patients receiving combined treatment displayed constant frequency and severity of symptoms of agitation/aggression (F-1,F-48 = 6.43; p <0.05) and depression (F-1,F-48 = 26.54; p <0.001). Symptom amelioration did not occur in either group. Conclusions: AAT is a promising option for the treatment of agitation/aggression and depression in patients with dementia. Our results suggest that AAT may delay progression of neuropsychiatric symptoms in demented nursing home residents. Further research is needed to determine its long-time effects.
Molecular mechanisms of desiccation tolerance in the resurrection glacial relic Haberlea rhodopensis
(2013)
Haberlea rhodopensis is a resurrection plant with remarkable tolerance to desiccation. Haberlea exposed to drought stress, desiccation, and subsequent rehydration showed no signs of damage or severe oxidative stress compared to untreated control plants. Transcriptome analysis by next-generation sequencing revealed a drought-induced reprogramming, which redirected resources from growth towards cell protection. Repression of photosynthetic and growth-related genes during water deficiency was concomitant with induction of transcription factors (members of the NAC, NF-YA, MADS box, HSF, GRAS, and WRKY families) presumably acting as master switches of the genetic reprogramming, as well as with an upregulation of genes related to sugar metabolism, signaling, and genes encoding early light-inducible (ELIP), late embryogenesis abundant (LEA), and heat shock (HSP) proteins. At the same time, genes encoding other LEA, HSP, and stress protective proteins were constitutively expressed at high levels even in unstressed controls. Genes normally involved in tolerance to salinity, chilling, and pathogens were also highly induced, suggesting a possible cross-tolerance against a number of abiotic and biotic stress factors. A notable percentage of the genes highly regulated in dehydration and subsequent rehydration were novel, with no sequence homology to genes from other plant genomes. Additionally, an extensive antioxidant gene network was identified with several gene families possessing a greater number of antioxidant genes than most other species with sequenced genomes. Two of the transcripts most abundant during all conditions encoded catalases and five more catalases were induced in water-deficient samples. Using the pharmacological inhibitor 3-aminotriazole (AT) to compromise catalase activity resulted in increased sensitivity to desiccation. Metabolome analysis by GC or LC-MS revealed accumulation of sucrose, verbascose, spermidine, and gamma-aminobutyric acid during drought, as well as particular secondary metabolites accumulating during rehydration. This observation, together with the complex antioxidant system and the constitutive expression of stress protective genes suggests that both constitutive and inducible mechanisms contribute to the extreme desiccation tolerance of H. rhodopensis.
Natural genetic diversity provides a powerful tool to study the complex interrelationship between metabolism and growth. Profiling of metabolic traits combined with network-based and statistical analyses allow the comparison of conditions and identification of sets of traits that predict biomass. However, it often remains unclear why a particular set of metabolites is linked with biomass and to what extent the predictive model is applicable beyond a particular growth condition. A panel of 97 genetically diverse Arabidopsis (Arabidopsis thaliana) accessions was grown in near-optimal carbon and nitrogen supply, restricted carbon supply, and restricted nitrogen supply and analyzed for biomass and 54 metabolic traits. Correlation-based metabolic networks were generated from the genotype-dependent variation in each condition to reveal sets of metabolites that show coordinated changes across accessions. The networks were largely specific for a single growth condition. Partial least squares regression from metabolic traits allowed prediction of biomass within and, slightly more weakly, across conditions (cross-validated Pearson correlations in the range of 0.27-0.58 and 0.21-0.51 and P values in the range of <0.001-<0.13 and <0.001-<0.023, respectively). Metabolic traits that correlate with growth or have a high weighting in the partial least squares regression were mainly condition specific and often related to the resource that restricts growth under that condition. Linear mixed-model analysis using the combined metabolic traits from all growth conditions as an input indicated that inclusion of random effects for the conditions improves predictions of biomass. Thus, robust prediction of biomass across a range of conditions requires condition-specific measurement of metabolic traits to take account of environment-dependent changes of the underlying networks.
Early detection of salt stress is vital for plant survival and growth. Still, the molecular processes controlling early salt stress perception and signaling are not fully understood. Here, we identified SALT-RESPONSIVE ERF1 (SERF1), a rice (Oryza sativa) transcription factor (TF) gene that shows a root-specific induction upon salt and hydrogen peroxide (H2O2) treatment. Loss of SERF1 impairs the salt-inducible expression of genes encoding members of a mitogen-activated protein kinase (MAPK) cascade and salt tolerance-mediating TFs. Furthermore, we show that SERF1-dependent genes are H2O2 responsive and demonstrate that SERF1 binds to the promoters of MAPK KINASE KINASE6 (MAP3K6), MAPK5, DEHYDRATION-RESPONSIVE ELEMENT BINDING2A (DREB2A), and ZINC FINGER PROTEIN179 (ZFP179) in vitro and in vivo. SERF1 also directly induces its own gene expression. In addition, SERF1 is a phosphorylation target of MAPK5, resulting in enhanced transcriptional activity of SERF1 toward its direct target genes. In agreement, plants deficient for SERF1 are more sensitive to salt stress compared with the wild type, while constitutive overexpression of SERF1 improves salinity tolerance. We propose that SERF1 amplifies the reactive oxygen species-activated MAPK cascade signal during the initial phase of salt stress and translates the salt-induced signal into an appropriate expressional response resulting in salt tolerance.
Developmental senescence is a coordinated physiological process in plants and is critical for nutrient redistribution from senescing leaves to newly formed sink organs, including young leaves and developing seeds. Progress has been made concerning the genes involved and the regulatory networks controlling senescence. The resulting complex metabolome changes during senescence have not been investigated in detail yet. Therefore, we conducted a comprehensive profiling of metabolites, including pigments, lipids, sugars, amino acids, organic acids, nutrient ions, and secondary metabolites, and determined approximately 260 metabolites at distinct stages in leaves and siliques during senescence in Arabidopsis (Arabidopsis thaliana). This provided an extensive catalog of metabolites and their spatiotemporal cobehavior with progressing senescence. Comparison with silique data provides clues to source-sink relations. Furthermore, we analyzed the metabolite distribution within single leaves along the basipetal sink-source transition trajectory during senescence. Ceramides, lysolipids, aromatic amino acids, branched chain amino acids, and stress-induced amino acids accumulated, and an imbalance of asparagine/aspartate, glutamate/glutamine, and nutrient ions in the tip region of leaves was detected. Furthermore, the spatiotemporal distribution of tricarboxylic acid cycle intermediates was already changed in the presenescent leaves, and glucosinolates, raffinose, and galactinol accumulated in the base region of leaves with preceding senescence. These results are discussed in the context of current models of the metabolic shifts occurring during developmental and environmentally induced senescence. As senescence processes are correlated to crop yield, the metabolome data and the approach provided here can serve as a blueprint for the analysis of traits and conditions linking crop yield and senescence.
Growth regulation is an important aspect of plant adaptation during environmental perturbations. Here, the role of MULTIPASS (OsMPS), an R2R3-type MYB transcription factor of rice, was explored. OsMPS is induced by salt stress and expressed in vegetative and reproductive tissues. Over-expression of OsMPS reduces growth under non-stress conditions, while knockdown plants display increased biomass. OsMPS expression is induced by abscisic acid and cytokinin, but is repressed by auxin, gibberellin and brassinolide. Growth retardation caused by OsMPS over-expression is partially restored by auxin application. Expression profiling revealed that OsMPS negatively regulates the expression of EXPANSIN (EXP) and cell-wall biosynthesis as well as phytohormone signaling genes. Furthermore, the expression of OsMPS-dependent genes is regulated by auxin, cytokinin and abscisic acid. Moreover, we show that OsMPS is a direct upstream regulator of OsEXPA4, OsEXPA8, OsEXPB2, OsEXPB3, OsEXPB6 and the endoglucanase genes OsGLU5 and OsGLU14. The multiple responses of OsMPS and its target genes to various hormones suggest an integrative function of OsMPS in the cross-talk between phytohormones and the environment to regulate adaptive growth.
Understanding metabolic acclimation of plants to challenging environmental conditions is essential for dissecting the role of metabolic pathways in growth and survival. As stresses involve simultaneous physiological alterations across all levels of cellular organization, a comprehensive characterization of the role of metabolic pathways in acclimation necessitates integration of genome-scale models with high-throughput data. Here, we present an integrative optimization-based approach, which, by coupling a plant metabolic network model and transcriptomics data, can predict the metabolic pathways affected in a single, carefully controlled experiment. Moreover, we propose three optimization-based indices that characterize different aspects of metabolic pathway behavior in the context of the entire metabolic network. We demonstrate that the proposed approach and indices facilitate quantitative comparisons and characterization of the plant metabolic response under eight different light and/or temperature conditions. The predictions of the metabolic functions involved in metabolic acclimation of Arabidopsis thaliana to the changing conditions are in line with experimental evidence and result in a hypothesis about the role of homocysteine-to-Cys interconversion and Asn biosynthesis. The approach can also be used to reveal the role of particular metabolic pathways in other scenarios, while taking into consideration the entirety of characterized plant metabolism.
Context. Globular clusters (GCs) are established emitters of high-energy (HE, 100 MeV < E < 100 GeV) gamma-ray radiation which could originate from the cumulative emission of the numerous millisecond pulsars (msPSRs) in the clusters’ cores or from inverse Compton (IC) scattering of relativistic leptons accelerated in the GC environment. These stellar clusters could also constitute a new class of sources in the very-high-energy (VHE, E > 100 GeV) gamma-ray regime, judging from the recent detection of a signal from the direction of Terzan 5 with the H.E.S.S. telescope array. Aims. To search for VHE gamma-ray sources associated with other GCs, and to put constraints on leptonic emission models, we systematically analyzed the observations towards 15 GCs taken with the H. E. S. S. array of imaging atmospheric Cherenkov telescopes. Methods. We searched for point-like and extended VHE gamma-ray emission from each GC in our sample and also performed a stacking analysis combining the data from all GCs to investigate the hypothesis of a population of faint emitters. Assuming IC emission as the origin of the VHE gamma-ray signal from the direction of Terzan 5, we calculated the expected gamma-ray flux from each of the 15 GCs, based on their number of millisecond pulsars, their optical brightness and the energy density of background photon fields. Results. We did not detect significant VHE gamma-ray emission from any of the 15 GCs in either of the two analyses. Given the uncertainties related to the parameter determinations, the obtained flux upper limits allow to rule out the simple IC/msPSR scaling model for NGC6388 and NGC7078. The upper limits derived from the stacking analyses are factors between 2 and 50 below the flux predicted by the simple leptonic scaling model, depending on the assumed source extent and the dominant target photon fields. Therefore, Terzan 5 still remains exceptional among all GCs, as the VHE gamma-ray emission either arises from extra-ordinarily efficient leptonic processes, or from a recent catastrophic event, or is even unrelated to the GC itself.
The quasar PKS 1510-089 (z = 0.361) was observed with the H.E.S.S. array of imaging atmospheric Cherenkov telescopes during high states in the optical and GeV bands, to search for very high energy (VHE, defined as E >= 0.1 TeV) emission. VHE gamma-rays were detected with a statistical significance of 9.2 standard deviations in 15.8 h of H. E. S. S. data taken during March and April 2009. A VHE integral flux of I(0.15 TeV < E < 1.0TeV) = (1.0 +/- 0.2(stat) +/- 0.2(sys)) x 10(-11) cm(-2) s(-1) is measured. The best-fit power law to the VHE data has a photon index of G = 5.4 +/- 0.7(stat) +/- 0.3(sys). The GeV and optical light curves show pronounced variability during the period of H.E.S.S. observations. However, there is insufficient evidence to claim statistically significant variability in the VHE data. Because of its relatively high redshift, the VHE flux from PKS 1510-089 should suffer considerable attenuation in the intergalactic space due to the extragalactic background light (EBL). Hence, the measured gamma-ray spectrum is used to derive upper limits on the opacity due to EBL, which are found to be comparable with the previously derived limits from relatively-nearby BL Lac objects. Unlike typical VHE-detected blazars where the broadband spectrum is dominated by nonthermal radiation at all wavelengths, the quasar PKS 1510-089 has a bright thermal component in the optical to UV frequency band. Among all VHE detected blazars, PKS 1510-089 has the most luminous broad line region. The detection of VHE emission from this quasar indicates a low level of gamma - gamma absorption on the internal optical to UV photon field.
Axionlike particles (ALPs) are hypothetical light (sub-eV) bosons predicted in some extensions of the Standard Model of particle physics. In astrophysical environments comprising high-energy gamma rays and turbulent magnetic fields, the existence of ALPs can modify the energy spectrum of the gamma rays for a sufficiently large coupling between ALPs and photons. This modification would take the form of an irregular behavior of the energy spectrum in a limited energy range. Data from the H. E. S. S. observations of the distant BL Lac object PKS 2155 - 304 (z = 0.116) are used to derive upper limits at the 95% C. L. on the strength of the ALP coupling to photons, g(gamma a) < 2.1 x 10(-11) GeV-1 for an ALP mass between 15 and 60 neV. The results depend on assumptions on the magnetic field around the source, which are chosen conservatively. The derived constraints apply to both light pseudoscalar and scalar bosons that couple to the electromagnetic field.
Gamma-ray line signatures can be expected in the very-high-energy (E-gamma > 100 GeV) domain due to self-annihilation or decay of dark matter (DM) particles in space. Such a signal would be readily distinguishable from astrophysical gamma-ray sources that in most cases produce continuous spectra that span over several orders of magnitude in energy. Using data collected with the H. E. S. S. gamma-ray instrument, upper limits on linelike emission are obtained in the energy range between similar to 500 GeV and similar to 25 TeV for the central part of the Milky Way halo and for extragalactic observations, complementing recent limits obtained with the Fermi-LAT instrument at lower energies. No statistically significant signal could be found. For monochromatic gamma-ray line emission, flux limits of (2 x 10(-7)-2 x 10(-5)) m(-2)s(-1)sr(-1) and (1 x 10(-8)- 2 x 10(-6)) m(-2)s(-1)sr(-1) are obtained for the central part of the Milky Way halo and extragalactic observations, respectively. For a DM particle mass of 1 TeV, limits on the velocity- averaged DM annihilation cross section <sigma upsilon >(chi chi ->gamma gamma) reach similar to 10(-27)cm(3)s(-1), based on the Einasto parametrization of the Galactic DM halo density profile. DOI: 10.1103/PhysRevLett.110.041301
Very high-energy gamma-ray emission from PKS 0447-439 was detected with the H. E. S. S. Cherenkov telescope array in December 2009. This blazar is one of the brightest extragalactic objects in the Fermi bright source list and has a hard spectrum in the MeV to GeV range. In the TeV range, a photon index of 3.89 +/- 0.37 (stat) +/- 0.22 (sys) and a flux normalisation at 1 TeV, phi(1) (TeV) = (3.5 +/- 1.1(stat) +/- 0.9(sys)) x 10(-13) cm(-2) s(-1) TeV-1 were found. The detection with H. E. S. S. triggered observations in the X-ray band with the Swift and RXTE telescopes. Simultaneous UV and optical data from Swift UVOT and data from the optical telescopes ATOM and ROTSE are also available. The spectrum and light curve measured with H. E. S. S. are presented and compared to the multi-wavelength data at lower energies. A rapid flare is seen in the Swift XRT and RXTE data, together with a flux variation in the UV band, at a time scale of the order of one day. A firm upper limit of z < 0.59 on the redshift of PKS 0447-439 is derived from the combined Fermi-LAT and H. E. S. S. data, given the assumptions that there is no upturn in the intrinsic spectrum above the Fermi-LAT energy range and that absorption on the extragalactic background light (EBL) is not weaker than the lower limit provided by current models. The spectral energy distribution is well described by a simple one-zone synchrotron self-Compton scenario, if the redshift of the source is less than z less than or similar to 0.4.
Discovery of high and very high-energy emission from the BL Lacertae object SHBL J001355.9-185406
(2013)
The detection of the high-frequency peaked BL Lac object (HBL) SHBL J001355.9-185406 (z = 0.095) at high (HE; 100 MeV < E < 300 GeV) and very high-energy (VHE; E > 100 GeV) with the Fermi Large Area Telescope (LAT) and the High Energy Stereoscopic System (H.E.S.S.) is reported. Dedicated observations were performed with the H. E. S. S. telescopes, leading to a detection at the 5.5 sigma significance level. The measured flux above 310 GeV is (8.3 +/- 1.7(stat) +/- 1.7(sys)) x 10(-13) photons cm(-2) s(-1) (about 0.6% of that of the Crab Nebula), and the power-law spectrum has a photon index of Gamma = 3.4 +/- 0.5(stat) +/- 0.2(sys). Using 3.5 years of publicly available Fermi-LAT data, a faint counterpart has been detected in the LAT data at the 5.5 sigma significance level, with an integrated flux above 300 MeV of (9.3 +/- 3.4(stat) +/- 0.8(sys)) x 10(-10) photons cm(-2) s(-1) and a photon index of Gamma = 1.96 +/- 0.20(stat) +/- 0.08(sys). X-ray observations with Swift-XRT allow the synchrotron peak energy in vF(v) representation to be located at similar to 1.0 keV. The broadband spectral energy distribution is modelled with a one-zone synchrotron self-Compton (SSC) model and the optical data by a black-body emission describing the thermal emission of the host galaxy. The derived parameters are typical of HBLs detected at VHE, with a particle-dominated jet.
Discovery of very high energy gamma-ray emission from the BL Lacertae
object PKS0301-243 with HESS
(2013)
The active galactic nucleus PKS 0301-243 (z = 0.266) is a high-synchrotron-peaked BL Lac object that is detected at high energies (HE, 100 MeV < E < 100 GeV) by Fermi/LAT. This paper reports on the discovery of PKS 0301-243 at very high energies (E > 100 GeV) by the High Energy Stereoscopic System (H.E.S.S.) from observations between September 2009 and December 2011 for a total live time of 34.9 h. Gamma rays above 200 GeV are detected at a significance of 9.4 sigma. A hint of variability at the 2.5 sigma level is found. An integral flux I(E > 200GeV) = (3.3 +/- 1.1(stat) +/- 0.7(syst)) x 10(-12) ph cm(-2) s(-1) and a photon index Gamma = 4.6 +/- 0.7(stat) +/- 0.2(syst) are measured. Multi-wavelength light curves in HE, X-ray and optical bands show strong variability, and a minimal variability timescale of eight days is estimated from the optical light curve. A single-zone leptonic synchrotron self-Compton scenario satisfactorily reproduces the multi-wavelength data. In this model, the emitting region is out of equipartition and the jet is particle dominated. Because of its high redshift compared to other sources observed at TeV energies, the very high energy emission from PKS 0301-243 is attenuated by the extragalactic background light (EBL) and the measured spectrum is used to derive an upper limit on the opacity of the EBL.
HESS observations of the binary system PSR B1259-63/LS 2883 around the 2010/2011 periastron passage
(2013)
Aims. We present very high energy (VHE; E > 100 GeV) data from the gamma-ray binary system PSR B1259-63/LS 2883 taken around its periastron passage on 15th of December 2010 with the High Energy Stereoscopic System (H. E. S. S.) of Cherenkov Telescopes. We aim to search for a possible TeV counterpart of the GeV flare detected by the Fermi LAT. In addition, we aim to study the current periastron passage in the context of previous observations taken at similar orbital phases, testing the repetitive behaviour of the source.
Methods. Observations at VHEs were conducted with H.E.S.S. from 9th to 16th of January 2011. The total dataset amounts to similar to 6 h of observing time. The data taken around the 2004 periastron passage were also re-analysed with the current analysis techniques in order to extend the energy spectrum above 3 TeV to fully compare observation results from 2004 and 2011.
Results. The source is detected in the 2011 data at a significance level of 11.5 sigma revealing an averaged integral flux above 1 TeV of (1.01 +/- 0.18(stat) +/- 0.20(sys)) x 10(-12) cm(-2) s(-1). The differential energy spectrum follows a power-law shape with a spectral index Gamma = 2.92 +/- 0.30(stat) +/- 0.20(sys) and a flux normalisation at 1 TeV of N-0 = (1.95 +/- 0.32(stat) +/- 0.39(sys)) x 10(-12) TeV-1 cm(-2) s(-1). The measured light curve does not show any evidence for variability of the source on the daily scale. The re-analysis of the 2004 data yields results compatible with the published ones. The differential energy spectrum measured up to similar to 10 TeV is consistent with a power law with a spectral index Gamma = 2.81 +/- 0.10(stat) +/- 0.20(sys) and a flux normalisation at 1 TeV of N-0 = (1.29 +/- 0.08(stat) +/- 0.26(sys)) x 10(-12) TeV-1 cm(-2) s(-1).
Conclusions. The measured integral flux and the spectral shape of the 2011 data are compatible with the results obtained around previous periastron passages. The absence of variability in the H.E.S.S. data indicates that the GeV flare observed by Fermi LAT in the time period covered also by H.E.S.S. observations originates in a different physical scenario than the TeV emission. Moreover, the comparison of the new results to the results from the 2004 observations made at a similar orbital phase provides a stronger evidence of the repetitive behaviour of the source.
A deep observation campaign carried out by the High Energy Stereoscopic System (HESS) on Centaurus A enabled the discovery of gamma-rays from the blazar 1ES 1312-423, 2 degrees away from the radio galaxy. With a differential flux at 1 TeV of phi(1 TeV) = (1.9 +/- 0.6(stat) +/- 0.4(sys)) x 10(-13) cm(-2) s(-1) TeV-1 corresponding to 0.5 per cent of the Crab nebula differential flux and a spectral index Gamma = 2.9 +/- 0.5(stat) +/- 0.2(sys), 1ES 1312-423 is one of the faintest sources ever detected in the very high energy (E > 100 GeV) extragalactic sky. A careful analysis using three and a half years of Fermi Large Area Telescope (Fermi-LAT) data allows the discovery at high energies (E > 100 MeV) of a hard spectrum (Gamma = 1.4 +/- 0.4(stat) +/- 0.2(sys)) source coincident with 1ES 1312-423. Radio, optical, UV and X-ray observations complete the spectral energy distribution of this blazar, now covering 16 decades in energy. The emission is successfully fitted with a synchrotron self-Compton model for the non-thermal component, combined with a blackbody spectrum for the optical emission from the host galaxy.