Filtern
Erscheinungsjahr
Dokumenttyp
- Wissenschaftlicher Artikel (107)
- Postprint (22)
- Konferenzveröffentlichung (3)
- Rezension (2)
- Sonstiges (1)
Gehört zur Bibliographie
- ja (135)
Schlagworte
- zinc (7)
- Arsenolipids (6)
- Caenorhabditis elegans (6)
- manganese (6)
- Arsenic-containing hydrocarbons (5)
- Boron exposure (5)
- C. elegans (5)
- Arsenic-containing fatty acids (4)
- Boric acid (4)
- DNA damage (4)
- DNA damage response (4)
- Manganese (4)
- Methylmercury (4)
- Toxicity (4)
- Zinc (4)
- arsenolipids present (4)
- cells (4)
- cod-liver (4)
- copper (4)
- electrochemistry (4)
- fatty-acids (4)
- homeostasis (4)
- identification (4)
- neurodegenerative diseases (4)
- neurotoxicity (4)
- Arsenic (3)
- Astrocytes (3)
- DNA repair (3)
- Development (3)
- Metabolism (3)
- Modified mycotoxins (3)
- Neurotoxicity (3)
- Pesticides (3)
- methylmercury (3)
- oxidative stress (3)
- selenium (3)
- transformation products (3)
- 2D-LC-MS/MS (2)
- Apoptosis (2)
- Arsenic speciation (2)
- Biomarker (2)
- Caco-2 intestinal barrier model (2)
- Caco-2/HT-29-MTX-model (2)
- Cereals (2)
- Copper (2)
- Cytotoxicity (2)
- Dopamine (2)
- Foxp3 (2)
- GPx activity (2)
- Genotoxicity (2)
- HRMS (2)
- ICP-MS (2)
- In vitro blood-brain barrier model (2)
- LC/HRMS (2)
- Labile zinc (2)
- Mercuric mercury (2)
- Multi-method (2)
- Mycotoxins (2)
- Nrf2 (2)
- Oxidative stress (2)
- Parkinson disease (2)
- RP-HPLC (2)
- S-XRF (2)
- SIMS techniques (2)
- Se (2)
- Selenium (2)
- TEM (2)
- Thiomersal (2)
- Thioredoxin (2)
- Trace elements (2)
- Zinc homeostasis (2)
- Zinypr-1 (2)
- adduct formation (2)
- ageing (2)
- aging (2)
- base excision repair (incision activity) (2)
- binding (2)
- biomarker (2)
- bladder-cancer (2)
- c. elegans (2)
- carcinogen exposure (2)
- cardiovascular disease (2)
- cell-death (2)
- cellular bioimaging (2)
- coenzyme-a (2)
- copper-related disorders (2)
- cytosine methylation (2)
- database (2)
- disease (2)
- energy-metabolism (2)
- excision-repair (2)
- exposure (2)
- fish (2)
- fluorescent probe (2)
- force-field (2)
- free zinc (2)
- gene-expression (2)
- genomic dna methylation (2)
- glutathione (2)
- goblet cells (2)
- hallervorden-spatz-syndrome (2)
- human-cells (2)
- hydrolysis (2)
- in vitro intestinal model (2)
- induced malignant-transformation (2)
- intestinal mucins (2)
- intestinal zinc resorption (2)
- iron (2)
- life-span (2)
- liver (2)
- maintenance of genomic integrity (2)
- marine oils (2)
- mass-spectrometry (2)
- menadione (2)
- metabolites (2)
- methyltransferases dnmt3a (2)
- mobility-mass spectrometry (2)
- model (2)
- monensin (2)
- mucus layer (2)
- n-acetyl-cysteine (2)
- neurodegeneration (2)
- oil (2)
- poly(ADP-ribose) polymerase-1 (2)
- poly(ADP-ribosyl)ation (2)
- protein (2)
- repair (2)
- rp-hplc (2)
- s-glutathionylation (2)
- selenoprotein P (2)
- serum (2)
- sex (2)
- status markers (2)
- thimerosal (2)
- thio-dimethylarsinic acid (2)
- trivalent (2)
- veterinary drugs (2)
- vitro toxicological characterization (2)
- zinc binding (2)
- AMD (1)
- ARPE-19 cells (1)
- Advanced glycation end products (1)
- Age (1)
- Aging (1)
- Akt/PKB (1)
- Antioxidant (1)
- Arsenite (1)
- Arsenolipid (1)
- Arsenosugar (1)
- Automation (1)
- Beef (1)
- Beer (1)
- Biochemistry (1)
- Biological Sciences (1)
- Biological monitoring (1)
- Blood-cerebrospinal fluid barrier (1)
- Blood-liquor barrier (1)
- Books (1)
- C (1)
- Casein (1)
- Cell culture materials (1)
- Cellular bioavailability (1)
- Cellular damage response (1)
- Cognition (1)
- Collagen (1)
- Comet assay (1)
- Contamination (1)
- Corylus avellana L. (1)
- Cu NP-incorporated MI-dPG coating (1)
- Cytokines (1)
- DAF-16 (1)
- DNA integrity (1)
- DNMT1 (1)
- Developmental toxicity (1)
- Dopaminergic neurons (1)
- ER stress (1)
- Elemental blood serum concentration (1)
- Environmental (1)
- Experimental autoimmune encephalomyelitis (EAE) (1)
- FSH (1)
- Food (1)
- Freeze-fracturing (1)
- GADD45A and GADD45G (1)
- GC-MS (1)
- GSH (1)
- Gene expression (1)
- Global DNA methylation (1)
- Glyphosate (1)
- Heavy metals (1)
- Human differentiated neurons (1)
- Human nutritional intervention (1)
- Hyphenated techniques (1)
- ICP-QQQ-MS (1)
- Inductively (1)
- Inductively coupled plasma mass spectrometry (1)
- Inert ingredients (1)
- Inflammation (1)
- Inorganic mercury (1)
- Isotope dilution analysis (1)
- Isotope ratios (1)
- Isotope-dilution analysis (1)
- Jod (1)
- Journals (1)
- LC (1)
- LC–MS/MS (1)
- LC−MS/MS (1)
- LH (1)
- LPS (1)
- Life science (1)
- Lipid (1)
- Liquid chromatography-tandem mass spectrometry (1)
- Liquid-liquid extraction (1)
- Lithiumion battery (LIB) (1)
- Long-term cellular toxicity (1)
- Lupin (1)
- Macrophage-like phenotype (1)
- Male (1)
- Manganese . C. elegans (1)
- Membrane (1)
- Mercury (1)
- Metals (1)
- Methylglyoxal (1)
- Microbiome (1)
- Mitochondria (1)
- Mixed lymphocyte culture (MLC) (1)
- Motor coordination (1)
- Multi-mycotoxin analysis (1)
- Neurodegeneration (1)
- Neurodevelopmental toxicity (1)
- Neurons (1)
- Nif2 (1)
- Occurrence data (1)
- Oocytes (1)
- Organic arsenic (1)
- Organic carbonates (1)
- Organic mercury (1)
- Paternal exposure (1)
- Pericytes (1)
- Pig (1)
- Pregnancy outcomes (1)
- Presystemic metabolism (1)
- QuEChERS (1)
- ROS (1)
- Redox homeostasis (1)
- Regulatory T cells (Treg) (1)
- Reproduction (1)
- Reproductive toxicity (1)
- SGK-1 (1)
- Schilddrüse (1)
- Schilddrüsenautoimmunerkrankungen (1)
- Science and Mathematics (1)
- Se-methylselenoneine (1)
- Seafood (1)
- Selen (1)
- Selenoneine (1)
- Selenoproteine (1)
- Selenosugar 1 (1)
- Semen parameters (1)
- Serotonin (1)
- Sex (1)
- Sex ratio at birth (1)
- Signaling pathways (1)
- Small molecules (1)
- Small selenium species (1)
- Speciation (1)
- T helper 17 cells (1)
- Testosterone (1)
- Th17 (1)
- Thimerosal (1)
- Thio-arsenosugar-glycerol (1)
- Thio-dimethylarsinic acid (1)
- Thioredoxin reductase (1)
- ToF-SIMS (1)
- ToF-SIMS imaging (1)
- Total arsenic (1)
- Toxicokinetics (1)
- TraceAge (1)
- Transmembrane asymmetry (1)
- Tween40 micelles (1)
- Uruguay River (1)
- Validation (1)
- Y:X chromosome ratio (1)
- antibacterial effect (1)
- antioxidant defense systems (1)
- bioavailability (1)
- caenorhabditis elegans (1)
- cancer stem cells (1)
- cereals (1)
- coupled plasma mass spectrometry (1)
- dendritic polyglycerol (1)
- drug-resistant bacteria (1)
- elegans (1)
- gold nanostars (1)
- hazelnut cultivars (1)
- healthy subjects (1)
- high-resolution imaging (1)
- human liver microsomes (1)
- in situ chemical reduction (1)
- in vitro blood-brain barrier model (1)
- inorganic mercury (1)
- ion chromatography (1)
- ion quantification (1)
- ionophore antibiotics (1)
- lipid analysis (1)
- lutein (1)
- membrane analysis (1)
- mercuric mercury (1)
- metabolic response (1)
- method development (1)
- method validation (1)
- minerals (1)
- modified mycotoxins (1)
- moxidectin (1)
- multi-mycotoxin analysis (1)
- nutrient composition (1)
- organic mercury (1)
- photochemistry (1)
- photothermal therapy (1)
- pollution (1)
- postprandial study (1)
- presystemic metabolism (1)
- qPCR-based gene expression screening (1)
- rapeseed protein (1)
- rat (1)
- regulatory T cells (1)
- retinoic acid (1)
- salinomycin (1)
- sample preparation (1)
- selenoneine (1)
- selenoproteins (1)
- sodium (1)
- soy protein (1)
- thiomersal (1)
- tocopherols (1)
- transformation product (1)
- universal coating (1)
- validation (1)
- veterinary drug (1)
- yield enhancement (1)
Uruguay River is the most important river in western Rio Grande do Sul, separating Brazil from Argentina and Uruguay. However, its pollution is of great concern due to agricultural activities in the region and the extensive use of pesticides. In a long term, this practice leads to environmental pollution, especially to the aquatic system. The objective of this study was to analyze the physicochemical characteristics, metals and pesticides levels in water samples obtained before and after the planting and pesticides' application season from three sites: Uruguay River and two minor affluents, Mezomo Dam and Salso Stream. For biomonitoring, the free-living nematode Caenorhabditis elegans was used, which were exposed for 24 h. We did not find any significant alteration in physicochemical parameters. In the pre- and post-pesticides' samples we observed a residual presence of three pesticides (tebuconazole, imazethapyr, and clomazone) and metals which levels were above the recommended (As, Hg, Fe, and Mn). Exposure to both pre- and post-pesticides' samples impaired C. elegans reproduction and post-pesticides samples reduced worms' survival rate and lifespan. PCA analysis indicated that the presence of metals and pesticides are important variables that impacted C. elegans biological endpoints. Our data demonstrates that Uruguay River and two affluents are contaminated independent whether before or after pesticides' application season. In addition, it reinforces the usefulness of biological indicators, since simple physicochemical analyses are not sufficient to attest water quality and ecological safety.
The existence of cancer stem cells (CSCs) poses a major obstacle for the success of current cancer therapies, especially the fact that non-CSCs can spontaneously turn into CSCs, which lead to the failure of the treatment and tumor relapse. Therefore, it is very important to develop effective strategies for the eradication of the CSCs. In this work, we have developed a CSCs-specific targeted, retinoic acid (RA)-loaded gold nanostars-dendritic polyglycerol (GNSs-dPG) nanoplatform for the efficient eradication of CSCs. The nanocomposites possess good biocompatibility and exhibit effective CSCs-specific multivalent targeted capability due to hyaluronic acid (HA) decorated on the multiple attachment sites of the bioinert dendritic polyglycerol (dPG). With the help of CSCs differentiation induced by RA, the self-renewal of breast CSCs and tumor growth were suppressed by the high therapeutic efficacy of photothermal therapy (PTT) in a synergistic inhibitory manner. Moreover, the stemness gene expression and CSC-driven tumorsphere formation were significantly diminished. In addition, the in vivo tumor growth and CSCs were also effectively eliminated, which indicated superior anticancer activity, effective CSCs suppression, and prevention of relapse. Taken together, we developed a CSCs-specific targeted, RA-loaded GNSs-dPG nanoplatform for the targeted eradication of CSCs and for preventing the relapse.
The Caenorhabditis elegans (C. elegans) is a model organism that has been increasingly used in health and environmental toxicity assessments. The quantification of such elements in vivo can assist in studies that seek to relate the exposure concentration to possible biological effects.
Therefore, this study is the first to propose a method of quantitative analysis of 21 ions by ion chromatography (IC), which can be applied in different toxicity studies in C. elegans.
The developed method was validated for 12 anionic species (fluoride, acetate, chloride, nitrite, bromide, nitrate, sulfate, oxalate, molybdate, dichromate, phosphate, and perchlorate), and 9 cationic species (lithium, sodium, ammonium, thallium, potassium, magnesium, manganese, calcium, and barium).
The method did not present the presence of interfering species, with R2 varying between 0.9991 and 0.9999, with a linear range from 1 to 100 mu g L-1.
Limits of detection (LOD) and limits of quantification (LOQ) values ranged from 0.2319 mu g L-1 to 1.7160 mu g L-1 and 0.7028 mu g L-1 to 5.1999 mu g L-1, respectively.
The intraday and interday precision tests showed an Relative Standard Deviation (RSD) below 10.0 % and recovery ranging from 71.0 % to 118.0 % with a maximum RSD of 5.5 %.
The method was applied to real samples of C. elegans treated with 200 uM of thallium acetate solution, determining the uptake and bioaccumulated Tl+ content during acute exposure.
Manganese (Mn) and zinc (Zn) are not only essential trace elements, but also potential exogenous risk factors for various diseases. Since the disturbed homeostasis of single metals can result in detrimental health effects, concerns have emerged regarding the consequences of excessive exposures to multiple metals, either via nutritional supplementation or parenteral nutrition. This study focuses on Mn-Zn-interactions in the nematode Caenorhabditis elegans (C. elegans) model, taking into account aspects related to aging and age-dependent neurodegeneration.
Mycotoxins and pesticides regularly co-occur in agricultural products worldwide. Thus, humans can be exposed to both toxic contaminants and pesticides simultaneously, and multi-methods assessing the occurrence of various food contaminants and residues in a single method are necessary. A two-dimensional high performance liquid chromatography tandem mass spectrometry method for the analysis of 40 (modified) mycotoxins, two plant growth regulators, two tropane alkaloids, and 334 pesticides in cereals was developed. After an acetonitrile/water/formic acid (79:20:1, v/v/v) multi-analyte extraction procedure, extracts were injected into the two-dimensional setup, and an online clean-up was performed. The method was validated according to Commission Decision (EC) no. 657/2002 and document N° SANTE/12682/2019. Good linearity (R2 > 0.96), recovery data between 70-120%, repeatability and reproducibility values < 20%, and expanded measurement uncertainties < 50% were obtained for a wide range of analytes, including very polar substances like deoxynivalenol-3-glucoside and methamidophos. However, results for fumonisins, zearalenone-14,16-disulfate, acid-labile pesticides, and carbamates were unsatisfying. Limits of quantification meeting maximum (residue) limits were achieved for most analytes. Matrix effects varied highly (−85 to +1574%) and were mainly observed for analytes eluting in the first dimension and early-eluting analytes in the second dimension. The application of the method demonstrated the co-occurrence of different types of cereals with 28 toxins and pesticides. Overall, 86% of the samples showed positive findings with at least one mycotoxin, plant growth regulator, or pesticide.
Mechanistic studies on the adverse effects of manganese overexposure in differentiated LUHMES cells
(2022)
Manganese (Mn) is an essential trace element, but overexposure is associated with toxicity and neurological dysfunction. Accumulation of Mn can be observed in dopamine-rich regions of the brain in vivo and Mn-induced oxidative stress has been discussed extensively. Nevertheless, Mn-induced DNA damage, adverse effects of DNA repair, and possible resulting consequences for the neurite network are not yet characterized. For this, LUHMES cells were used, as they differentiate into dopaminergic-like neurons and form extensive neurite networks. Experiments were conducted to analyze Mn bioavailability and cytotoxicity of MnCl2, indicating a dose-dependent uptake and substantial cytotoxic effects. DNA damage, analyzed by means of 8-oxo-7,8-dihydro-2'-guanine (8oxodG) and single DNA strand break formation, showed significant dose- and time-dependent increase of DNA damage upon 48 h Mn exposure. Furthermore, the DNA damage response was increased which was assessed by analytical quantification of poly(ADP-ribosyl)ation (PARylation). Gene expression of the respective DNA repair genes was not significantly affected. Degradation of the neuronal network is significantly altered by 48 h Mn exposure. Altogether, this study contributes to the characterization of Mn-induced neurotoxicity, by analyzing the adverse effects of Mn on genome integrity in dopaminergic-like neurons and respective outcomes.
Plant proteins have become increasingly important for ecological reasons. Rapeseed is a novel source of plant proteins with high biological value, but its metabolic impact in humans is largely unknown. A randomized, controlled intervention study including 20 healthy subjects was conducted in a crossover design. All participants received a test meal without additional protein or with 28 g of rapeseed protein isolate or soy protein isolate (control). Venous blood samples were collected over a 360-min period to analyze metabolites; satiety was assessed using a visual analog scale. Postprandial levels of lipids, urea, and amino acids increased following the intake of both protein isolates. The postprandial insulin response was lower after consumption of the rapeseed protein than after intake of the soy protein (p< 0.05), whereas the postmeal responses of glucose, lipids, interleukin-6, minerals, and urea were comparable between the two protein isolates. Interestingly, the rapeseed protein exerted stronger effects on postprandial satiety than the soy protein (p< 0.05). The postmeal metabolism following rapeseed protein intake is comparable with that of soy protein. The favorable effect of rapeseed protein on postprandial insulin and satiety makes it a valuable plant protein for human nutrition.
The drug salinomycin (SAL) is a polyether antibiotic and used in veterinary medicine as coccidiostat and growth promoter. Recently, SAL was suggested as a potential anticancer drug. However, transformation products (TPs) resulting from metabolic and environmental degradation of SAL are incompletely known and structural information is missing. In this study, we therefore systematically investigated the formation and identification of SAL derived TPs using electrochemistry (EC) in an electrochemical reactor and rat and human liver microsome incubation (RLM and HLM) as TP generating methods. Liquid chromatography (LC) coupled to high-resolution mass spectrometry (HRMS) was applied to determine accurate masses in a suspected target analysis to identify TPs and to deduce occurring modification reactions of derived TPs. A total of 14 new, structurally different TPs were found (two EC-TPs, five RLM-TPs, and 11 HLM-TPs). The main modification reactions are decarbonylation for EC-TPs and oxidation (hydroxylation) for RLM/HLM-TPs. Of particular interest are potassium-based TPs identified after liver microsome incubation because these might have been overlooked or declared as oxidated sodium adducts in previous, non-HRMS-based studies due to the small mass difference between K and O + Na of 21 mDa. The MS fragmentation pattern of TPs was used to predict the position of identified modifications in the SAL molecule. The obtained knowledge regarding transformation reactions and novel TPs of SAL will contribute to elucidate SAL-metabolites with regards to structural prediction.
Background: Being an essential trace element, copper is involved in diverse physiological processes. However, excess levels might lead to adverse effects. Disrupted copper homeostasis, particularly in the brain, has been associated with human diseases including the neurodegenerative disorders Wilson and Alzheimer?s disease. In this context, astrocytes play an important role in the regulation of the copper homeostasis in the brain and likely in the prevention against neuronal toxicity, consequently pointing them out as a potential target for the neurotoxicity of copper. Major toxic mechanisms are discussed to be directed against mitochondria probably via oxidative stress. However, the toxic potential and mode of action of copper in astrocytes is poorly understood, so far. Methods: In this study, excess copper levels affecting human astrocytic cell model and their involvement in the neurotoxic mode of action of copper, as well as, effects on the homeostasis of other trace elements (Mn, Fe, Ca and Mg) were investigated. Results: Copper induced substantial cytotoxic effects in the human astrocytic cell line following 48 h incubation (EC30: 250 ?M) and affected mitochondrial function, as observed via reduction of mitochondrial membrane potential and increased ROS production, likely originating from mitochondria. Moreover, cellular GSH metabolism was altered as well. Interestingly, not only cellular copper levels were affected, but also the homeostasis of other elements (Ca, Fe and Mn) were disrupted. Conclusion: One potential toxic mode of action of copper seems to be effects on the mitochondria along with induction of oxidative stress in the human astrocytic cell model. Moreover, excess copper levels seem to interact with the homeostasis of other essential elements such as Ca, Fe and Mn. Disrupted element homeostasis might also contribute to the induction of oxidative stress, likely involved in the onset and progression of neurodegenerative disorders. These insights in the toxic mechanisms will help to develop ideas and approaches for therapeutic strategies against copper-mediated diseases.