Refine
Language
- English (13)
Is part of the Bibliography
- yes (13)
Keywords
- mesenchymal stem cells (4)
- HDAC1 (2)
- RUNX2 (2)
- calcium influx (2)
- reversible shape-memory actuator (2)
- Actuation (1)
- Adipocyte (1)
- Antiviral (1)
- Biomaterial (1)
- Biomaterials (1)
- COVID-19 (1)
- Langmuir-Schafer films (1)
- Poly(n-butyl acrylate) (1)
- Polyether ether ketone (1)
- Polymeric substrate (1)
- Shape memory (1)
- VEGF (1)
- amorphous polymers (1)
- basement membrane (1)
- biocompatibility (1)
- bioinstructive materials (1)
- cardiac regeneration (1)
- cell adhesion (1)
- cell culture device (1)
- cell-material interaction (1)
- cold (1)
- collagen-IV (1)
- dedifferentiation (1)
- dendritic cells (1)
- ellipsometry (1)
- function by structure; (1)
- induced pluripotent stem cells (1)
- lipid (1)
- mechanical property (1)
- modulation of in vivo regeneration (1)
- multifunctional biomaterials (1)
- osteogenic differentiation (1)
- protein (1)
- stem cell adhesion (1)
- surface coating (1)
- vascular graft (1)
Institute
- Institut für Chemie (13) (remove)
Rapid migration of mesenchymal stem cells (MSCs) on device surfaces could support in vivo tissue integration and might facilitate in vitro organoid formation. Here, polydopamine (PDA) is explored as a biofunctional coating to effectively promote MSC motility. It is hypothesized that PDA stimulates fibronectin deposition and in this way enhances integrin-mediated migration capability. The random and directional cell migration was investigated by time-lapse microscopy and gap closure assay respectively, and analysed with softwares as computational tools. A higher amount of deposited fibronectin was observed on PDA substrate, compared to the non-coated substrate. The integrin beta 1 activation and focal adhesion kinase (FAK) phosphorylation at Y397 were enhanced on PDA substrate, but the F-actin cytoskeleton was not altered, suggesting MSC migration on PDA was regulated by integrin initiated FAK signalling. This study strengthens the biofunctionality of PDA coating for regulating stem cells and offering a way of facilitating tissue integration of devices.
Toll-like receptor (TLR) can trigger an immune response against virus including SARS-CoV-2. TLR expression/distribution is varying in mesenchymal stromal cells (MSCs) depending on their culture environments. Here, to explore the effect of periodic thermomechanical cues on TLRs, thermally controlled shape-memory polymer sheets with programmable actuation capacity were created. The proportion of MSCs expressing SARS-CoV-2-associated TLRs was increased upon stimulation. The TLR4/7 colocalization was promoted and retained in the endoplasmic reticula. The TLR redistribution was driven by myosin-mediated F-actin assembly. These results highlight the potential of boosting the immunity for combating COVID-19 via thermomechanical preconditioning of MSCs.
Guidance of postinfarct myocardial remodeling processes by an epicardial patch system may alleviate the consequences of ischemic heart disease. As macrophages are highly relevant in balancing immune response and regenerative processes their suitable instruction would ensure therapeutic success. A polymeric mesh capable of attracting and instructing monocytes by purely physical cues and accelerating implant degradation at the cell/implant interface is designed. In a murine model for myocardial infarction the meshes are compared to those either coated with extracellular matrix or loaded with induced cardiomyocyte progenitor cells. All implants promote macrophage infiltration and polarization in the epicardium, which is verified by in vitro experiments. 6 weeks post-MI, especially the implantation of the mesh attenuates left ventricular adverse remodeling processes as shown by reduced infarct size (14.7% vs 28-32%) and increased wall thickness (854 mu m vs 400-600 mu m), enhanced angiogenesis/arteriogenesis (more than 50% increase compared to controls and other groups), and improved heart function (ejection fraction = 36.8% compared to 12.7-31.3%). Upscaling as well as process controls is comprehensively considered in the presented mesh fabrication scheme to warrant further progression from bench to bedside.