- Honey traceability is an important topic, especially for honeydew honeys, due to the increased incidence of adulteration. This study aimed to establish specific markers to quantify proteins in honey. A proteomics strategy to identify marker peptides from bracatinga honeydew honey was therefore developed. The proteomics approach was based on initial untargeted identification of honey proteins and peptides by LC-ESI-Triple-TOF-MS/MS, which identified the major royal jelly proteins (MRJP) presence. Afterwards, the peptides were selected by the in silico digestion. The marker peptides were quantified by the developed targeted LC-QqQ-MS/MS method, which provided good linearity and specificity, besides recoveries between 92 and 100% to quantify peptides from bracatinga honeydew honey. The uniqueness and high response in mass spectrometry were backed by further complementary protein analysis (SDS-PAGE). The selected marker peptides EALPHVPIFDR (MRJP 1), ILGANVK (MRJP 2), TFVTIER (MRJP 3), QNIDVVAR (MRJP 4), FINNDYNFNEVNFR (MRJP 5) andHoney traceability is an important topic, especially for honeydew honeys, due to the increased incidence of adulteration. This study aimed to establish specific markers to quantify proteins in honey. A proteomics strategy to identify marker peptides from bracatinga honeydew honey was therefore developed. The proteomics approach was based on initial untargeted identification of honey proteins and peptides by LC-ESI-Triple-TOF-MS/MS, which identified the major royal jelly proteins (MRJP) presence. Afterwards, the peptides were selected by the in silico digestion. The marker peptides were quantified by the developed targeted LC-QqQ-MS/MS method, which provided good linearity and specificity, besides recoveries between 92 and 100% to quantify peptides from bracatinga honeydew honey. The uniqueness and high response in mass spectrometry were backed by further complementary protein analysis (SDS-PAGE). The selected marker peptides EALPHVPIFDR (MRJP 1), ILGANVK (MRJP 2), TFVTIER (MRJP 3), QNIDVVAR (MRJP 4), FINNDYNFNEVNFR (MRJP 5) and LLQPYPDWSWTK (MRJP 7), quantified by LC-QqQ-MS/MS, highlighted that the content of QNIDVVAR from MRJP 4 could be used to differentiate bracatinga honeydew honey from floral honeys (p < 0.05) as a potential marker for its authentication. Finally, principal components analysis highlighted the QNIDVVAR content as a good descriptor of the analyzed bracatinga honeydew honey samples.…
MetadatenAuthor details: | Bibiana SilvaORCiD, Ana Carolina Oliveira CostaORCiD, Sorel Sagu TchewonpiORCiD, Josephine Bönick, Gerd HuschekGND, Luciano Valdemiro GonzagaORCiD, Roseane FettORCiD, Susanne BaldermannORCiDGND, Harshadrai Manilal RawelORCiDGND |
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DOI: | https://doi.org/10.1016/j.foodres.2020.109991 |
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ISSN: | 0963-9969 |
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ISSN: | 1873-7145 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/33641949 |
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Title of parent work (English): | Food research international |
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Publisher: | Elsevier |
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Place of publishing: | New York, NY [u.a.] |
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Publication type: | Article |
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Language: | English |
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Date of first publication: | 2020/12/16 |
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Publication year: | 2021 |
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Release date: | 2024/05/30 |
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Tag: | High-resolution mass spectrometry; Honeydew honey; Major royal jelly proteins; Marker peptides; Principal component analysis |
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Volume: | 141 |
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Article number: | 109991 |
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Number of pages: | 10 |
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Funding institution: | Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) -BrazilCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES); Deutscher Akademischer Austauschdienst (DAAD) GermanyDeutscher Akademischer Austausch Dienst (DAAD) [23038.005155/2018-48]; Alexander von Humboldt Foundation under the Georg Forster Research FellowshipAlexander von Humboldt Foundation [3.4 - CMR -1164093 - GF-P]; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq, BrazilConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) [153639/2019-9] |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft |
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DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
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| 6 Technik, Medizin, angewandte Wissenschaften / 63 Landwirtschaft / 630 Landwirtschaft und verwandte Bereiche |
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| 6 Technik, Medizin, angewandte Wissenschaften / 64 Hauswirtschaft und Familie / 640 Hauswirtschaft und Familie |
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| 6 Technik, Medizin, angewandte Wissenschaften / 66 Chemische Verfahrenstechnik / 660 Chemische Verfahrenstechnik |
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Peer review: | Referiert |
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