- Objective: A role for microRNAs is implicated in several biological and pathological processes. We investigated the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on molecular markers of diabetic cardiomyopathy in rats.
Methods: Eighteen male Wistar rats (260 ± 10 g; aged 8 weeks) with streptozotocin (STZ)-induced type 1 diabetes mellitus (55 mg/kg, IP) were randomly allocated to three groups: control, MICT, and HIIT. The two different training protocols were performed 5 days each week for 5 weeks. Cardiac performance (end-systolic and end-diastolic dimensions, ejection fraction), the expression of miR-206, HSP60, and markers of apoptosis (cleaved PARP and cytochrome C) were determined at the end of the exercise interventions.
Results: Both exercise interventions (HIIT and MICT) decreased blood glucose levels and improved cardiac performance, with greater changes in the HIIT group (p < 0.001, η2: 0.909). While the expressions of miR-206 and apoptotic markers decreased in bothObjective: A role for microRNAs is implicated in several biological and pathological processes. We investigated the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on molecular markers of diabetic cardiomyopathy in rats.
Methods: Eighteen male Wistar rats (260 ± 10 g; aged 8 weeks) with streptozotocin (STZ)-induced type 1 diabetes mellitus (55 mg/kg, IP) were randomly allocated to three groups: control, MICT, and HIIT. The two different training protocols were performed 5 days each week for 5 weeks. Cardiac performance (end-systolic and end-diastolic dimensions, ejection fraction), the expression of miR-206, HSP60, and markers of apoptosis (cleaved PARP and cytochrome C) were determined at the end of the exercise interventions.
Results: Both exercise interventions (HIIT and MICT) decreased blood glucose levels and improved cardiac performance, with greater changes in the HIIT group (p < 0.001, η2: 0.909). While the expressions of miR-206 and apoptotic markers decreased in both training protocols (p < 0.001, η2: 0.967), HIIT caused greater reductions in apoptotic markers and produced a 20% greater reduction in miR-206 compared with the MICT protocol (p < 0.001). Furthermore, both training protocols enhanced the expression of HSP60 (p < 0.001, η2: 0.976), with a nearly 50% greater increase in the HIIT group compared with MICT.
Conclusions: Our results indicate that both exercise protocols, HIIT and MICT, have the potential to reduce diabetic cardiomyopathy by modifying the expression of miR-206 and its downstream targets of apoptosis. It seems however that HIIT is even more effective than MICT to modulate these molecular markers.…
MetadatenVerfasserangaben: | Maryam DelfanORCiD, Raheleh Amadeh JuybariORCiD, Sattar Gorgani-FiruzjaeeORCiD, Jens Høiriis NielsenORCiDGND, Neda DelfanORCiD, Ismail LaherORCiDGND, Ayoub SaeidiORCiD, Urs GranacherORCiDGND, Hassane ZouhalORCiD |
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DOI: | https://doi.org/10.3389/fcvm.2022.927956 |
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ISSN: | 2297-055X |
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Titel des übergeordneten Werks (Englisch): | Frontiers in Cardiovascular Medicine |
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Verlag: | Frontiers |
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Verlagsort: | Lausanne, Schweiz |
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Sonstige beteiligte Person(en): | Jian Shi, Dongtak Jeong, Shun Yan, Pingzhu Zhou |
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Publikationstyp: | Wissenschaftlicher Artikel |
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Sprache: | Englisch |
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Datum der Erstveröffentlichung: | 29.06.2022 |
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Erscheinungsjahr: | 2022 |
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Datum der Freischaltung: | 17.11.2022 |
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Freies Schlagwort / Tag: | apoptosis; cardiomyopathy; diabetes; exercise; miRNAs |
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Band: | 9 |
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Aufsatznummer: | 927956 |
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Seitenanzahl: | 11 |
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Erste Seite: | 1 |
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Letzte Seite: | 11 |
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Organisationseinheiten: | Extern / Extern |
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| Humanwissenschaftliche Fakultät / Strukturbereich Kognitionswissenschaften |
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DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Peer Review: | Referiert |
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Fördermittelquelle: | Publikationsfonds der Universität Potsdam |
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Publikationsweg: | Open Access / Gold Open-Access |
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Lizenz (Deutsch): | CC-BY - Namensnennung 4.0 International |
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Externe Anmerkung: | Zweitveröffentlichung in der Schriftenreihe Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe ; 802 |
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