Co-delivery of genes can be confounded by bicistronic vector design
- Maximizing the efficiency of nanocarrier-mediated co-delivery of genes for co-expression in the same cell is critical for many applications. Strategies to maximize co-delivery of nucleic acids (NA) focused largely on carrier systems, with little attention towards payload composition itself. Here, we investigated the effects of different payload designs: co-delivery of two individual "monocistronic" NAs versus a single bicistronic NA comprising two genes separated by a 2A self-cleavage site. Unexpectedly, co-delivery via the monocistronic design resulted in a higher percentage of co-expressing cells, while predictive co-expression via the bicistronic design remained elusive. Our results will aid the application-dependent selection of the optimal methodology for co-delivery of genes.
| Author details: | Hanieh MoradianORCiDGND, Manfred GossenORCiDGND, Andreas LendleinORCiDGND |
|---|---|
| DOI: | https://doi.org/10.1557/s43579-021-00128-7 |
| ISSN: | 2159-6859 |
| ISSN: | 2159-6867 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/35223145 |
| Title of parent work (English): | MRS Communications |
| Publisher: | Springer |
| Place of publishing: | Heidelberg |
| Publication type: | Article |
| Language: | English |
| Date of first publication: | 2022/02/18 |
| Publication year: | 2022 |
| Release date: | 2023/06/08 |
| Tag: | Molecular; Packaging; Protein |
| Volume: | 12 |
| Issue: | 2 |
| Number of pages: | 9 |
| First page: | 145 |
| Last Page: | 153 |
| Funding institution: | Helmholtz Association; Helmholtz Graduate School of Macromolecular; Bioscience (MacroBio) [VH-GS-503]; German Federal Ministry of Education; and Research through the Program Health Research [13GW0098] |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
| DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| Peer review: | Referiert |
| Publishing method: | Open Access / Hybrid Open-Access |
| License (German): |  CC-BY - Namensnennung 4.0 International |
