The hit-and-return system enables efficient time-resolved serial synchrotron crystallography

We present a ‘hit-and-return’ (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.

Metadaten
Author details:	Eike C. Schulz, Pedram Mehrabi ORCiD, Henrike Müller-Werkmeister ORCiD GND, Friedjof Tellkamp ORCiD, Ajay Jha ORCiD, William Stuart, Elke Persch GND, Raoul De Gasparo GND, François Diederich GND, Emil F. Pai ORCiD, R. J. Dwayne Miller ORCiD GND
DOI:	https://doi.org/10.1038/s41592-018-0180-2
ISSN:	1548-7091
ISSN:	1548-7105
Pubmed ID:	https://pubmed.ncbi.nlm.nih.gov/30377366
Title of parent work (English):	Nature methods : techniques for life scientists and chemists
Publisher:	Nature Publishing Group (London)
Place of publishing:	London
Publication type:	Article
Language:	English
Date of first publication:	2018/10/30
Publication year:	2018
Release date:	2021/07/19
Tag:	Biophysical chemistry; Enzymes; Molecular biophysics; X-ray crystallography
Volume:	15
Issue:	11
Number of pages:	6
First page:	901
Last Page:	904
Organizational units:	Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
DDC classification:	5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Peer review:	Referiert
Publishing method:	Open Access / Green Open-Access