The Role of the Nucleotides in the Insertion of the bis-Molybdopterin Guanine Dinucleotide Cofactor into apo-Molybdoenzymes
- The role of the GMP nucleotides of the bis-molybdopterin guanine dinucleotide (bis-MGD) cofactor of the DMSO reductase family has long been a subject of discussion. The recent characterization of the bis-molybdopterin (bis-Mo-MPT) cofactor present in the E. coli YdhV protein, which differs from bis-MGD solely by the absence of the nucleotides, now enables studying the role of the nucleotides of bis-MGD and bis-MPT cofactors in Moco insertion and the activity of molybdoenzymes in direct comparison. Using the well-known E. coli TMAO reductase TorA as a model enzyme for cofactor insertion, we were able to show that the GMP nucleotides of bis-MGD are crucial for the insertion of the bis-MGD cofactor into apo-TorA.
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| Author details: | Kim Tiedemann, Chantal Iobbi-NivolORCiD, Silke LeimkühlerORCiDGND |
|---|---|
| URN: | urn:nbn:de:kobv:517-opus4-561728 |
| DOI: | https://doi.org/10.25932/publishup-56172 |
| ISSN: | 1866-8372 |
| Title of parent work (German): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe |
| Publication series (Volume number): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (1268) |
| Publisher: | Universitätsverlag Potsdam |
| Place of publishing: | Potsdam |
| Further contributing person(s): | Roberto Fernandez-Lafuente |
| Publication type: | Postprint |
| Language: | English |
| Date of first publication: | 2022/09/27 |
| Publication year: | 2022 |
| Publishing institution: | Universität Potsdam |
| Release date: | 2022/09/27 |
| Tag: | TMAO reductase; bis-MGD; chaperone; molybdenum cofactor |
| Number of pages: | 15 |
| First page: | 1 |
| Last Page: | 15 |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| Peer review: | Referiert |
| Publishing method: | Open Access / Green Open-Access |
| License (German): |  CC-BY - Namensnennung 4.0 International |
| External remark: | Bibliographieeintrag der Originalveröffentlichung/Quelle |
