Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation
- In vitro thrombogenicity test systems require co-cultivation of endothelial cells and platelets under blood flow-like conditions. Here, a commercially available perfusion system is explored using plasma-treated cyclic olefin copolymer (COC) as a substrate for the endothelial cell layer. COC was characterized prior to endothelialization and co-cultivation with platelets under static or flow conditions. COC exhibits a low roughness and a moderate hydrophilicity. Flow promoted endothelial cell growth and prevented platelet adherence. These findings show the suitability of COC as substrate and the importance of blood flow-like conditions for the assessment of the thrombogenic risk of drugs or cardiovascular implant materials.
| Author details: | Skadi LauGND, Yue LiuORCiDGND, Anna MaierGND, Steffen BrauneORCiDGND, Manfred GossenORCiDGND, Axel T. NeffeORCiDGND, Andreas LendleinORCiDGND |
|---|---|
| DOI: | https://doi.org/10.1557/s43579-021-00072-6 |
| ISSN: | 2159-6867 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/34513262 |
| Title of parent work (English): | MRS communications / a publication of the Materials Research Society |
| Publisher: | Springer |
| Place of publishing: | Berlin |
| Publication type: | Article |
| Language: | English |
| Date of first publication: | 2021/09/07 |
| Publication year: | 2021 |
| Release date: | 2024/06/13 |
| Volume: | 11 |
| Issue: | 5 |
| Number of pages: | 9 |
| First page: | 559 |
| Last Page: | 567 |
| Funding institution: | Projekt DEAL |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
| DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| Peer review: | Referiert |
| Publishing method: | Open Access / Hybrid Open-Access |
| License (German): |  CC-BY - Namensnennung 4.0 International |
