Binding and degradation of juvenile hormone III by haemolymph proteins of the Colorado potato beetle: a re-examination
- The haemolymph of the adult Colorado potato beetle, Lepinotarsa decemlineata Say, contains a high molecular weight (MW > 200,000) JH-III specific binding protein. The Kd value of the protein for racemic JH-III is 1.3 ± 0.2 × 10−7 M. It has a lower affinity for racemic JH-I and it does not bind JH-III-diol or JH-III-acid. The binding protein does discriminate between the enantiomers of synthetic, racemic JH-III as was determined by stereochemical anaysis of the bound and the free JH-III. Incubation of racemic JH-III with crude haemolymph results in preferential formation of (10S)-JH-III-acid, the unnatural configuration. The JH-esterase present in L. decemlineata haemolymph is not enantioselective. It is concluded that the most important function of the binding protein is that of a specific carrier, protecting the natural hormone against degradation by esterases. The carrier does not protect JH-I as efficiently as the lower homologue.
Verfasserangaben: | C. A. D. de Kort, Martin G. Peter, A. B. Koopmanschap |
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URN: | urn:nbn:de:kobv:517-opus-16777 |
Schriftenreihe (Bandnummer): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (paper 068) |
Publikationstyp: | Postprint |
Sprache: | Englisch |
Erscheinungsjahr: | 1983 |
Veröffentlichende Institution: | Universität Potsdam |
Datum der Freischaltung: | 27.08.2008 |
Freies Schlagwort / Tag: | JH-III-specific carrier protein; Juvenile hormone; Leptinotarsa decemlineata; enantioselectivity |
Quelle: | Insect Biochemistry. - 13 (1983), 5, pp. 481 - 487. ISSN 0020-1790 |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
Extern / Extern | |
DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Lizenz (Deutsch): | Keine öffentliche Lizenz: Unter Urheberrechtsschutz |
Externe Anmerkung: | first published in: Insect Biochemistry. - 13 (1983), 5, pp. 481 - 487 ISSN 0020-1790 DOI 10.1016/0020-1790(83)90005-7 |