Improvement of a fluorescence immunoassay with a compact diode-pumped solid state laser at 315 nm
- We demonstrate the improvement of fluorescence immunoassay (FIA) diagnostics in deploying a newly developed compact diode-pumped solid state (DPSS) laser with emission at 315 nm. The laser is based on the quasi-three-level transition in Nd:YAG at 946 nm. The pulsed operation is either realized by an active Q-switch using an electro-optical device or by introduction of a Cr<SUP>4+</SUP>:YAG saturable absorber as passive Q-switch element. By extra-cavity second harmonic generation in different nonlinear crystal media we obtained blue light at 473 nm. Subsequent mixing of the fundamental and the second harmonic in a β-barium-borate crystal provided pulsed emission at 315 nm with up to 20 μJ maximum pulse energy and 17 ns pulse duration. Substitution of a nitrogen laser in a FIA diagnostics system by the DPSS laser succeeded in considerable improvement of the detection limit. Despite significantly lower pulse energies (7 μJ DPSS laser versus 150 μJ nitrogen laser), in preliminary investigations the limit of detection was reduced by aWe demonstrate the improvement of fluorescence immunoassay (FIA) diagnostics in deploying a newly developed compact diode-pumped solid state (DPSS) laser with emission at 315 nm. The laser is based on the quasi-three-level transition in Nd:YAG at 946 nm. The pulsed operation is either realized by an active Q-switch using an electro-optical device or by introduction of a Cr<SUP>4+</SUP>:YAG saturable absorber as passive Q-switch element. By extra-cavity second harmonic generation in different nonlinear crystal media we obtained blue light at 473 nm. Subsequent mixing of the fundamental and the second harmonic in a β-barium-borate crystal provided pulsed emission at 315 nm with up to 20 μJ maximum pulse energy and 17 ns pulse duration. Substitution of a nitrogen laser in a FIA diagnostics system by the DPSS laser succeeded in considerable improvement of the detection limit. Despite significantly lower pulse energies (7 μJ DPSS laser versus 150 μJ nitrogen laser), in preliminary investigations the limit of detection was reduced by a factor of three for a typical FIA.…
| Verfasserangaben: | Matthias Niederkrüger, Christian Salb, Michael Beck, Niko HildebrandtGND, Hans-Gerd LöhmannsröbenORCiDGND, Gerd Marowsky |
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| URN: | urn:nbn:de:kobv:517-opus-10150 |
| Schriftenreihe (Bandnummer): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (paper 016) |
| Publikationstyp: | Postprint |
| Sprache: | Englisch |
| Erscheinungsjahr: | 2006 |
| Veröffentlichende Institution: | Universität Potsdam |
| Datum der Freischaltung: | 01.11.2006 |
| Freies Schlagwort / Tag: | 315 nm; 473 nm; 946 nm; gepulster DPSS Laser; sättigbarer Absorber 315 nm; 473 nm; 946 nm; fluorescence immunoassay; pulsed DPSS laser |
| GND-Schlagwort: | Immunoassay; Fluoreszenz-Resonanz-Energie-Transfer; Neodym-YAG-Laser |
| Quelle: | Proc. SPIE, Vol. 6380 (2006) 63800M |
| RVK - Regensburger Verbundklassifikation: | VE 9908 |
| Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
| Extern / Extern | |
| DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| Externe Anmerkung: | © 2006 Society of Photo-Optical Instrumentation Engineers This paper was published in: Smart Medical and Biomedical Sensor Technology IV / Brian M. Cullum, J. Chance Carter (eds.), Proceedings of SPIE, Vol. 6380 (2006) ISBN: 0-8194-6478-3 It is made available as an electronic reprint with permission of SPIE. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purpose, or modification of the content of the paper are prohibited. |
