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Characterisation of odorant compounds and their biochemical formation in green tea with a low temperature storage process

  • We produced low temperature (15 degrees C) processed green tea (LTPGT) with higher aroma contents than normal green tea (Sencha). Normal temperature processed green tea (NTPGT), involved storing at 25 degrees C, and Sencha had no storing process. Sensory evaluation showed LTPGT had higher levels of floral and sweet odorants than NTPGT and Sencha. Aroma extract dilution analysis and gas chromatography-mass spectrometry-olfactometry indicated LTPGT had 12 aroma compounds with high factor dilution values (FD). Amongst LTPGT's 12 compounds, indole, jasmine lactone, cis-jasmone, coumarin, and methyl epijasmonate contributed to floral, fruity and sweet characters. In particular, indole increased initially, peaking at 16 h, then gradually decreased; Feeding experiments suggested [N-15]indole and [N-15]oxygenated indoles (OX-indoles) were produced from [N-15]anthranilic acid. We proposed the increase in indole was due to transformation of anthranilic acid during the 16 h storage and the subsequent decline in indole level was due to itsWe produced low temperature (15 degrees C) processed green tea (LTPGT) with higher aroma contents than normal green tea (Sencha). Normal temperature processed green tea (NTPGT), involved storing at 25 degrees C, and Sencha had no storing process. Sensory evaluation showed LTPGT had higher levels of floral and sweet odorants than NTPGT and Sencha. Aroma extract dilution analysis and gas chromatography-mass spectrometry-olfactometry indicated LTPGT had 12 aroma compounds with high factor dilution values (FD). Amongst LTPGT's 12 compounds, indole, jasmine lactone, cis-jasmone, coumarin, and methyl epijasmonate contributed to floral, fruity and sweet characters. In particular, indole increased initially, peaking at 16 h, then gradually decreased; Feeding experiments suggested [N-15]indole and [N-15]oxygenated indoles (OX-indoles) were produced from [N-15]anthranilic acid. We proposed the increase in indole was due to transformation of anthranilic acid during the 16 h storage and the subsequent decline in indole level was due to its conversion to OX-indoles.show moreshow less

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Author details:Tsuyoshi Katsuno, Hisae Kasuga, Yumi Kusano, Yoshihiro Yaguchi, Miho Tomomura, Jilai Cui, Ziyin Yang, Susanne BaldermannORCiDGND, Yoriyuki Nakamura, Toshiyuki Ohnishi, Nobuyuki Mase, Naoharu Watanabe
DOI:https://doi.org/10.1016/j.foodchem.2013.10.069
ISSN:0308-8146
ISSN:1873-7072
Pubmed ID:https://pubmed.ncbi.nlm.nih.gov/24262573
Title of parent work (English):Food chemistry
Publisher:Elsevier
Place of publishing:Oxford
Publication type:Article
Language:English
Year of first publication:2014
Publication year:2014
Release date:2017/03/27
Tag:Camellia sinensis; Indole; Metabolome analysis; Odorant compounds; Tea leaves; [N-15]Anthranilic acid; [N-15]Indole
Volume:148
Number of pages:8
First page:388
Last Page:395
Funding institution:Japan Science and Technology Agency (JST)
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft
Peer review:Referiert
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